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ins-cre_cryaa-Venus

Plasmid

#24333

Depositor

Didier Stainier

Article

Hesselson et al Proc Natl Acad Sci U S A. 2009 Sep 1. 10

Insert

Cre (cre )

Use

Cre/Lox

Tags

Venus

Expression

Worm

Available Since

April 27, 2010

Availability

Academic Institutions and Nonprofits only

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FGFR1-optoDroplet

Plasmid

#111509

Purpose

For optogenetic clustering of FGFR1

Depositor

Jared Toettcher

Article

Dine et al Cell Syst. 2018 May 24. pii: S2405-4712(

Insert

FGFR1 (FGFR1 Human)

Use

Lentiviral

Tags

Cry2, FUS, Fusion Red, and Myristoylation sequenc…

Promoter

SFFV

Available Since

June 14, 2018

Availability

Academic Institutions and Nonprofits only

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pMSSM110: pCMV-HA-NLS-SpuFz1-v2

Plasmid

#205277

Purpose

Human expression vector for human codon optimized N-terminal NLS-tagged SpuFz1-v2

Depositor

Feng Zhang

Article

Saito et al Nature. 2023 Jun 28. doi: 10.1038/s41586

Insert

SpuFz1

Tags

HA, NLS(Makkerh)

Expression

Mammalian

Mutation

C310R/D487K/T513K

Available Since

Aug. 8, 2023

Availability

Academic Institutions and Nonprofits only

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pRK5-mFzd6

Plasmid

#42258

Depositor

Chris Garcia, Jeremy Nathans

Article

Yu et al Development. 2012 Dec 1;139(23):4383-94.

Insert

Frizzled 6 (Fzd6 Mouse)

Expression

Mammalian

Available Since

March 29, 2013

Availability

Academic Institutions and Nonprofits only
pRK5-mFzd9

Plasmid

#42261

Depositor

Chris Garcia, Jeremy Nathans

Article

Yu et al Development. 2012 Dec 1;139(23):4383-94.

Insert

Frizzled 9 (Fzd9 Mouse)

Expression

Mammalian

Available Since

March 28, 2013

Availability

Academic Institutions and Nonprofits only
pFUSE-rIgG-Fc2-ICAM1

Plasmid

#156462

Purpose

Expresses a rabbit Fc-ICAM1 fusion protein in mammalian cells

Depositor

Dirk Mielenz

Article

Reimer et al Cell Rep. 2020 Aug 11;32(6):108030. doi:

Insert

Intercellular Adhesion Molecule 1 (Icam1 Mouse)

Tags

ICAM1-rFc

Expression

Bacterial and Mammalian

Mutation

endogenous signal peptide replaced with IL2 signa…

Promoter

hEF1-HTLC

Available Since

Oct. 13, 2020

Availability

Academic Institutions and Nonprofits only

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pcDNA3.1_ADAR2DD_E488Q

Plasmid

#205598

Purpose

expresses human ADAR2 deaminase domain (E488Q hyperactive mutant) in mammalian cells

Depositor

Marc Friedlander

Article

Sekar et al Nat Biotechnol. 2023 Sep 21. doi: 10.103

Insert

ADAR2 deaminase domain (ADARB1 Human)

Expression

Mammalian

Mutation

E488Q hyperactive mutation

Available Since

Oct. 2, 2023

Availability

Academic Institutions and Nonprofits only

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pAC103-PBNeo-U6-sgRNA-Expression

Plasmid

#48228

Purpose

sgRNA expression on PiggyBac vector with Neo-selectable marker

Depositor

Rudolf Jaenisch

Article

Cheng et al Cell Res. 2013 Aug 27. doi: 10.1038/cr.2

Type

Empty backbone

Use

CRISPR

Expression

Mammalian

Promoter

U6

Available Since

April 5, 2016

Availability

Academic Institutions and Nonprofits only

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pRK5-mWnt7a

Plasmid

#42282

Depositor

Chris Garcia, Jeremy Nathans

Article

Yu et al Development. 2012 Dec 1;139(23):4383-94.

Insert

Wnt7a (Wnt7a Mouse)

Expression

Mammalian

Available Since

March 28, 2013

Availability

Academic Institutions and Nonprofits only
pDsRed-miR9 Sensor

Plasmid

#22742

Depositor

Lynn Hudson

Article

Lau et al J Neurosci. 2008 Nov 5. 28(45):11720-30.

Insert

microRNA 9 binding sites

Expression

Mammalian

Available Since

Jan. 22, 2010

Availability

Academic Institutions and Nonprofits only

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pdestMB14_sur5p-dCas9-VP64

Plasmid

#177788

Purpose

Overexpression of dCas9-VP64 in C. elegans

Depositor

Michael Ristow

Article

Fischer et al J Biol Chem. 2022 May 27:102085. doi: 10

Insert

dCas9-VP64

Expression

Worm

Available Since

June 1, 2022

Availability

Academic Institutions and Nonprofits only

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Burden Monitor lambda version

Plasmid

#66073

Purpose

The burden monitor was cloned inside plasmid pAH63 [Haldimann2001]; the genome integration of the system allows the measurement of the burden imposed by synthetic constructs to Escherichia coli cells

Depositor

Tom Ellis

Article

Ceroni et al Nat Methods. 2015 May;12(5):415-8. doi:

Insert

GFP

Available Since

Dec. 21, 2015

Availability

Academic Institutions and Nonprofits only
pRK5-mFzd7-1D4

Plasmid

#42269

Depositor

Chris Garcia, Jeremy Nathans

Article

Yu et al Development. 2012 Dec 1;139(23):4383-94.

Insert

Frizzled 7 (Fzd7 Mouse)

Tags

1D4

Expression

Mammalian

Available Since

March 28, 2013

Availability

Academic Institutions and Nonprofits only
RCAS(A) mTcf4B (CT#1126)

Plasmid

#14019

Depositor

Cliff Tabin

Article

Kardon et al Dev Cell. 2003 Dec . 5(6):937-44.

Insert

Tcf4B (Tcf7l2 Mouse)

Use

Retroviral; Avian expression

Available Since

Feb. 16, 2007

Availability

Academic Institutions and Nonprofits only
pCMV-eNme2-T.2-ABE8e

Plasmid

#185669

Purpose

Expresses eNme2-T.2-ABE8e in mammalian cells

Depositor

David Liu

Article

Huang et al Nat Biotechnol. 2022 Sep 8. pii: 10.1038

Insert

ecTadA(8e)-neNme2-T.2

Use

CRISPR

Expression

Mammalian

Mutation

Nme2Cas9 D16A/E47K/R63K/V68M/A116T/T123A/D152N/E1…

Available Since

Sept. 28, 2022

Availability

Academic Institutions and Nonprofits only

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pCEP4-myc-PDGFRbeta

Plasmid

#136455

Purpose

Mammalian expression plasmid for c-myc-tagged PDGFRB

Depositor

Erik Procko

Article

Park et al PLoS Pathog. 2020 Jun 19;16(6):e1008647.

Insert

PDGFRbeta (PDGFRB Human)

Tags

Extracellular c-myc epitope tag and Signal peptid…

Expression

Mammalian

Promoter

CMV

Available Since

July 9, 2020

Availability

Academic Institutions and Nonprofits only

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mCherry-CRY2-INPP5E

Plasmid

#79561

Purpose

expression of mCherry-tagged CRY2PHR fused to inositol 5-phosphatase domain of human INPP5E with mutated C-terminal CaaX-motif

Depositor

Pietro De Camilli, Olof Idevall-Hagren

Article

Idevall-Hagren et al Proc Natl Acad Sci U S A. 2012 Aug 28;10

Insert

INPP5E (INPP5E Human)

Tags

CRY2 (photolyase homology region domain of Arabid…

Expression

Mammalian

Mutation

C-terminal region, aa 214_644, C641A mutation to …

Promoter

CMV

Available Since

July 11, 2016

Availability

Academic Institutions and Nonprofits only
Kras (G12V)-pcw107

Plasmid

#64602

Purpose

when used to produce lentivirus, express physiological levels of insert

Depositor

David Sabatini, Kris Wood

Article

Martz et al Sci Signal. 2014 Dec 23;7(357):ra121. do

Insert

KRAS (KRAS Human)

Use

Lentiviral

Mutation

G12V

Promoter

PGK

Available Since

May 12, 2015

Availability

Academic Institutions and Nonprofits only

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pLX307-CRBN-3F-BirA

Plasmid

#220146

Purpose

Expresses CRBN-BirA for CRBN substrate identification by E-STUB

Depositor

William Sellers

Article

Huang et al Nat Chem Biol. 2024 Mar 21. doi: 10.1038

Insert

Cereblon (CRBN Human)

Use

Lentiviral

Tags

3F-BirA

Expression

Mammalian

Promoter

EF-1a

Available Since

May 29, 2024

Availability

Academic Institutions and Nonprofits only

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pAS_sfGPF 150TAG

Plasmid

#154765

Purpose

plasmid with amber suppression reporter sfGFP 150 TAG stop, for transient transfection or stable, piggybac-mediated, integration

Depositor

Simon Elsaesser

Article

Meineke et al ACS Chem Biol. 2018 Sep 27. doi: 10.1021

Insert

sfGFP

Expression

Mammalian

Mutation

150TAG

Promoter

EF1

Available Since

Aug. 26, 2020

Availability

Academic Institutions and Nonprofits only

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