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Plasmid#169739PurposeUnregulated "open-loop" CMV promoter plasmid. This plasmid encodes eGFP to report circuit output levels.DepositorInserteGFP
UseSynthetic BiologyTagsExpressionMammalianMutationPromoterCMVAvailable sinceJune 7, 2021AvailabilityAcademic Institutions and Nonprofits only -
pET30_Halotag_K73T_v2
Plasmid#183692PurposeBacterial expresssion of ER-targeted metastable Halotag/folding sensor HaloTag (K73T)DepositorInsertER Halo(K73T)
UseTagsExpressionBacterialMutationK73T in HalotagPromoterAvailable sinceApril 14, 2023AvailabilityAcademic Institutions and Nonprofits only -
PCDNA3-N3FLAG-MYB-FL
Plasmid#105609Purposetransient express full length MYB with 3*FLAG tag at N terminal in HEK 293T cellsDepositorInsertFull length MYB (mouse) (Myb Mouse)
UseTags3*FLAGExpressionMammalianMutationPromoterCMVAvailable sinceFeb. 21, 2018AvailabilityAcademic Institutions and Nonprofits only -
SP_H6_Halo_K73T_KDEL_pBABEpu
Plasmid#183689PurposeRetroviral expression of ER-targeted metastable Halotag/folding sensor (HaloK73T)DepositorInsertER Halo(K73T)
UseRetroviralTagsExpressionMutationK73T in HalotagPromoterAvailable sinceApril 14, 2023AvailabilityAcademic Institutions and Nonprofits only -
Pr-deGFP-MGapt
Plasmid#67734PurposeOR2-OR1-Pr promoter expressing deGFP-MG aptamer fusionDepositorInsertUTR1-deGFP-MGapt-T500
UseSynthetic BiologyTagsExpressionBacterialMutationPromoterOR2-OR1-PrAvailable sinceAug. 6, 2015AvailabilityAcademic Institutions and Nonprofits only -
pIRES.Puro.mBeta
Plasmid#24175DepositorInsertpIRES.Puro.mBeta (Polb Mouse)
UseTagsExpressionMammalianMutationxPromoterAvailable sinceApril 9, 2010AvailabilityAcademic Institutions and Nonprofits only -
pTC-CMV-Tet
Plasmid#85577PurposeSleeping beauty transposon for hydrodynamic tail vein injection for CreER expression and tetracyclin-dependent transgene/shRNA expression (CMV promoter) in mouse liverDepositorInsertCreER
UseCre/LoxTagsExpressionMammalianMutationPromoterApoE.HCR.hAATAvailable sinceJan. 9, 2017AvailabilityAcademic Institutions and Nonprofits only -
pBacPak-HA-E(y)2
Plasmid#52288PurposeExpresses Drosophila E(y)2DepositorInsertE(y)2 (e(y)2 Fly)
UseTagsHAExpressionInsectMutationPromoterAvailable sinceApril 24, 2014AvailabilityAcademic Institutions and Nonprofits only -
pET28a-TAF4(827-909)
Plasmid#105620Purposebacterially express TAF4 HFDDepositorInsertTAF4-HFD(827-909) (Taf4 Mouse)
UseTagsExpressionBacterialMutationPromoterAvailable sinceFeb. 20, 2018AvailabilityAcademic Institutions and Nonprofits only -
pGEX-4T1-TAF12(50-130)
Plasmid#105618Purposebacterially express GST tagged TAF12 HFDDepositorInsertTAF12-HFD amino acid 50-130 (Taf12 Mouse)
UseTagsGSTExpressionBacterialMutationPromoterAvailable sinceFeb. 20, 2018AvailabilityAcademic Institutions and Nonprofits only -
Pr-deCFP-MGapt
Plasmid#67740PurposeOR2-OR1-Pr promoter expressing deCFP-MG aptamer fusionDepositorInsertUTR1-deCFP-MGapt-T500
UseSynthetic BiologyTagsExpressionBacterialMutationPromoterOR2-OR1-PrAvailable sinceAug. 7, 2015AvailabilityAcademic Institutions and Nonprofits only -
Pr1-T7RNAP
Plasmid#67738PurposeOR2-OR1-Pr1 promoter expressing T7 RNAPDepositorInsertUTR1-T7RNAP-T500
UseSynthetic BiologyTagsExpressionBacterialMutationPromoterOR2-OR1-Pr1Available sinceAug. 7, 2015AvailabilityAcademic Institutions and Nonprofits only -
Pr2-T7RNAP
Plasmid#67739PurposeOR2-OR1-P2 promoter expressing T7 RNAPDepositorInsertUTR1-T7RNAP-T500
UseSynthetic BiologyTagsExpressionBacterialMutationPromoterOR2-OR1-Pr2Available sinceAug. 7, 2015AvailabilityAcademic Institutions and Nonprofits only -
pGCaMP5
Plasmid#78705PurposeGCaMP5 under the Toxoplasma SAG1 5' UTR. No Toxoplasma selectable markerDepositorInsertGCaMP5
UseTagsExpressionMutationPromoterSAG1 5' UTRAvailable sinceMay 27, 2016AvailabilityAcademic Institutions and Nonprofits only -
pLLOXmPOLBeta
Plasmid#24174DepositorInsertpLLOXmPOLbeta (Polb Mouse)
UseLentiviralTagsExpressionMammalianMutationxPromoterAvailable sinceMay 27, 2010AvailabilityAcademic Institutions and Nonprofits only -
pHL 1454
Plasmid#53019PurposeAmpR + PLlacO-1::RBS (st7) fimB::Asp terminator + ColE1DepositorInsertfimB
UseSynthetic BiologyTagsRBS(st7)ExpressionBacterialMutationPromoterpLlac0-1Available sinceMay 14, 2014AvailabilityAcademic Institutions and Nonprofits only -
Pr-delRBS-deGFP
Plasmid#67744PurposePr promoter expressing a deGFP that is missing a ribosomal binding siteDepositorInsertdeGFP-T500
UseSynthetic BiologyTagsExpressionBacterialMutationPromoterOR2-OR1-PrAvailable sinceAug. 7, 2015AvailabilityAcademic Institutions and Nonprofits only -
pHL 1916
Plasmid#53023PurposeAmpR + PLtetO-1::RBS (st7) fimB::Asp terminator + ColE1DepositorInsertfimB
UseSynthetic BiologyTagsAsp terminator and RBS(st7)ExpressionBacterialMutationPromoterpLtetO-1Available sinceMay 14, 2014AvailabilityAcademic Institutions and Nonprofits only -
U6-miR.FF4
Plasmid#235256PurposeNon-intronic (U6-driven) expression of miR-FF4 (FF4 hairpin)DepositorInsertFF4 hairpin
UseSynthetic BiologyTagsExpressionMammalianMutationPromoterU6Available sinceMay 30, 2025AvailabilityAcademic Institutions and Nonprofits only -
pUB_SM-FLuc0-GFPOPT
Plasmid#210594PurposeExpresses SM(FLAG)-nonoptimal Firefly luciferase-optimal GFPDepositorInsertSpaghetti monster (FLAG)-nonoptimal FLuc-Optimal GFP
UseLuciferaseTagsSpaghetti monster (FLAG)ExpressionMammalianMutationPromoterUBC humanAvailable sinceOct. 23, 2024AvailabilityAcademic Institutions and Nonprofits only -
pUB_SM-Fluc100-GFPOPT
Plasmid#210596PurposeExpresses SM(FLAG)-optimal Firefly luciferase-optimal GFPDepositorInsertSpaghetti monster (FLAG)-optimal FLuc-Optimal GFP
UseLuciferaseTagsSpaghetti monster (FLAG)ExpressionMammalianMutationPromoterUBC humanAvailable sinceOct. 23, 2024AvailabilityAcademic Institutions and Nonprofits only -
pUB_SMFLuc0---MS2
Plasmid#210568PurposeExpresses SM(FLAG)-nonoptimal Firefly with 3'UTR MS2 hairpinsDepositorInsertSpaghetti monster (FLAG)-nonoptimal FLuc
UseLentiviral and LuciferaseTagsSpaghetti monster (FLAG)ExpressionMammalianMutationPromoterUBC humanAvailable sinceOct. 23, 2024AvailabilityAcademic Institutions and Nonprofits only -
pUB_SMFLuc100---MS2
Plasmid#210570PurposeExpresses SM(FLAG)-optimal Firefly with 3'UTR MS2 hairpinsDepositorInsertSpaghetti monster (FLAG)-optimal FLuc
UseLentiviral and LuciferaseTagsSpaghetti monster (FLAG)ExpressionMammalianMutationPromoterUBC humanAvailable sinceOct. 23, 2024AvailabilityAcademic Institutions and Nonprofits only -
pUB_SM-RLuc0---MS2
Plasmid#210572PurposeExpresses SM(FLAG)-nonoptimal Renilla with 3'UTR MS2 hairpinsDepositorInsertSpaghetti monster (FLAG)-nonoptimal Renilla luciferase
UseLentiviral and LuciferaseTagsSpaghetti monster (FLAG)ExpressionMammalianMutationPromoterUBC humanAvailable sinceOct. 23, 2024AvailabilityAcademic Institutions and Nonprofits only -
pUB_SM-RLuc100---MS2
Plasmid#210573PurposeExpresses SM(FLAG)-optimal Renilla with 3'UTR MS2 hairpinsDepositorInsertSpaghetti monster (FLAG)-optimal Renilla luciferase
UseLentiviral and LuciferaseTagsSpaghetti monster (FLAG)ExpressionMammalianMutationPromoterUBC humanAvailable sinceOct. 23, 2024AvailabilityAcademic Institutions and Nonprofits only -
pcDNA3.1_SM-FLuc0-malat1
Plasmid#210580PurposeExpresses SM(FLAG)-nonoptimal Firefly - 3'UTR malat1 triple helixDepositorInsertSpaghetti monster (FLAG)-nonoptimal FLuc - 3'UTR malat1 triple helix
UseLuciferaseTagsSpaghetti monster (FLAG)ExpressionMammalianMutationPromoterCMVAvailable sinceOct. 23, 2024AvailabilityAcademic Institutions and Nonprofits only -
pcDNA3.1_SM-FLuc100-malat1
Plasmid#210581PurposeExpresses SM(FLAG)-optimal Firefly luciferase - 3'UTR malat1 triple helixDepositorInsertSpaghetti monster (FLAG)-optimal FLuc - 3'UTR malat1 triple helix
UseLuciferaseTagsSpaghetti monster (FLAG)ExpressionMammalianMutationPromoterCMVAvailable sinceOct. 23, 2024AvailabilityAcademic Institutions and Nonprofits only -
pLX-SFFV_NKX2-1_Short_V5-GFP
Plasmid#221500PurposeNKX2-1-GFP fusion protein lentiviral overexpression using an SFFV promoterDepositorInsertNKX2-1 (NKX2-1 Human)
UseLentiviralTagsV5-linker-GFPExpressionMammalianMutationPromoterSFFVAvailable sinceJuly 2, 2024AvailabilityAcademic Institutions and Nonprofits only -
pLX-EF1a_NKX2-1_CdTag-HA
Plasmid#221493PurposeNKX2-1 short isoform lentiviral overexpression of C-terminal dTag fusionDepositorInsertNKX2-1 (NKX2-1 Human)
UseLentiviralTagsFKBP F36VExpressionMammalianMutationPromoterEF1aAvailable sinceJuly 2, 2024AvailabilityAcademic Institutions and Nonprofits only -
pDONR223_NKX2-1_DelCTerm_1-223AA
Plasmid#221476PurposeGateway cloning of NKX2-1 C-terminal truncation mutation overexpressionDepositorInsertNKX2-1 (NKX2-1 Human)
UseGateway donor vectorTagsExpressionMutationDeletion of 224-371AA C-terminal domainPromoterAvailable sinceJuly 2, 2024AvailabilityAcademic Institutions and Nonprofits only -
pDONR223_NKX2-1_DelNTerm_160-371AA
Plasmid#221475PurposeGateway cloning of NKX2-1 N-terminal truncation mutation overexpressionDepositorInsertNKX2-1 (NKX2-1 Human)
UseGateway donor vectorTagsExpressionMutationDeletion of 1-159AA N-terminal domainPromoterAvailable sinceJuly 2, 2024AvailabilityAcademic Institutions and Nonprofits only -
pDONR223_NKX2-1_HD_160-223AA
Plasmid#221477PurposeGateway cloning of NKX2-1 N-terminal and C-terminal truncation (homeodomain only) mutation overexpressionDepositorInsertNKX2-1 (NKX2-1 Human)
UseGateway donor vectorTagsExpressionMutationDeletion of 1-159AA N-terminal and 224-371AA C-te…PromoterAvailable sinceJuly 2, 2024AvailabilityAcademic Institutions and Nonprofits only -
pLX-EF1a_NKX2-1_DelNTerm_160-371AA
Plasmid#221487PurposeNKX2-1 N-terminal truncation mutation lentiviral overexpressionDepositorInsertNKX2-1 (NKX2-1 Human)
UseLentiviralTagsV5ExpressionMammalianMutationDeletion of 1-159AA N-terminal domainPromoterEF1aAvailable sinceJuly 1, 2024AvailabilityAcademic Institutions and Nonprofits only -
pLX-hPGK_NKX2-1_Short_V5-GFP
Plasmid#221498PurposeNKX2-1-GFP fusion protein lentiviral overexpression using an hPGK promoterDepositorInsertNKX2-1 (NKX2-1 Human)
UseLentiviralTagsV5-linker-GFPExpressionMammalianMutationPromoterhPGKAvailable sinceJuly 1, 2024AvailabilityAcademic Institutions and Nonprofits only -
pLX-EF1a_NKX2-1_DelCTerm_1-223AA
Plasmid#221488PurposeNKX2-1 C-terminal truncation mutation lentiviral overexpressionDepositorInsertNKX2-1 (NKX2-1 Human)
UseLentiviralTagsV5ExpressionMammalianMutationDeletion of 224-371AA C-terminal domainPromoterEF1aAvailable sinceJuly 1, 2024AvailabilityAcademic Institutions and Nonprofits only -
G1088E_pLenti-CMV-mNeonGreen-2A-HygroR
Plasmid#216279PurposeGreen and hygromycin resistant lentiviral vector plasmid for multiplexed receptor pseudovirus experimentsDepositorInsertmNeonGreen-2A-Hpt
UseLentiviralTagsExpressionMutationPromoterCMVAvailable sinceMay 23, 2024AvailabilityAcademic Institutions and Nonprofits only -
SP_H6_Halo_L172Q_KDEL_pBABEpu
Plasmid#183690PurposeRetroviral expression of ER-targeted metastable Halotag/folding sensor (ER HaloL172Q)DepositorInsertER Halo(L172Q)
UseRetroviralTagsExpressionMutationL72Q in HalotagPromoterAvailable sinceApril 14, 2023AvailabilityAcademic Institutions and Nonprofits only -
SP_H6_Halo_M21K_F86L_KDEL_pBABEpu
Plasmid#183691PurposeRetroviral expression of ER-targeted metastable Halotag/folding sensor (ER HaloM21K_F86L)DepositorInsertER Halo(M21K_F86L)
UseRetroviralTagsExpressionMutationM21K_F86L in HalotagPromoterAvailable sinceApril 14, 2023AvailabilityAcademic Institutions and Nonprofits only -
pET30_Halotag_K73T_Jdomain
Plasmid#183693PurposeBacterial expression of ER-targeted metastable Halotag/folding sensor (HaloK73T) fused with J domainDepositorInsertER Halo(K73T)-J domain
UseTagsExpressionBacterialMutationK73T in HalotagPromoterAvailable sinceApril 14, 2023AvailabilityAcademic Institutions and Nonprofits only -
Negative feedback (NF) plasmid
Plasmid#169747PurposeStandalone NF circuit plasmid that has the same implementation of the NF subcircuit in Equalizers.DepositorInserttetR-P2A-eGFP
UseSynthetic BiologyTagsExpressionMammalianMutationPromoterCMV-tetO2Available sinceJune 9, 2021AvailabilityAcademic Institutions and Nonprofits only