We narrowed to 59,321 results for: cloning

1. pJT216 Lenti pEF-dCas9-3XFLAG-LibCloneSite-T2A-tagBFP-P2A-Blast

   Plasmid

   #187320

   Purpose

   dCas9 expression vector with cloning site for C terminal fusions

   Depositor

   Lacra Bintu, Michael Bassik

   Article

   Tycko et al Nat Biotechnol. 2024 Nov 1. doi: 10.1038

   Type

   Empty backbone

   Use

   CRISPR and Lentiviral

   Tags

   3XFLAG

   Expression

   Mammalian

   Available Since

   May 6, 2024

   Availability

   Academic Institutions and Nonprofits only

   

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2. pET C-terminal TEV His6 cloning vector with BioBrick polycistronic restriction sites (9Bc)

   Plasmid

   #48285

   Depositor

   Scott Gradia

   Type

   Empty backbone

   Tags

   His6

   Expression

   Bacterial

   Available Since

   Nov. 5, 2013

   Availability

   Academic Institutions and Nonprofits only

   

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3. pFastBac His6 MBP TEV cloning vector with BioBrick polycistronic restriction sites (11C)

   Plasmid

   #48296

   Depositor

   Scott Gradia

   Article

   Gradia et al Methods Enzymol. 2017;592:1-26. doi: 10.

   Type

   Empty backbone

   Tags

   His6 and MBP

   Expression

   Insect

   Available Since

   Sept. 23, 2013

   Availability

   Academic Institutions and Nonprofits only

   

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4. pET His6 msfGFP TEV cloning vector with BioBrick polycistronic restriction sites (9GFP)

   Plasmid

   #48287

   Depositor

   Scott Gradia

   Type

   Empty backbone

   Tags

   His6 and msfGFP

   Expression

   Bacterial

   Available Since

   Nov. 6, 2013

   Availability

   Academic Institutions and Nonprofits only

   

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5. CAG-lox-CAT-lox-unique Bam cloning site-hGH polyA (Clone 17)

   Plasmid

   #53959

   Purpose

   allows Cre-mediated expression of transgene

   Depositor

   Jeffrey Robbins

   Article

   Nakamura et al Proc Natl Acad Sci U S A. 2009 Sep 8;106

   Type

   Empty backbone

   Use

   Cre/Lox

   Tags

   Lox-CAT-Lox

   Expression

   Mammalian

   Promoter

   CMV enhancer, chicken actin promoter

   Available Since

   June 30, 2014

   Availability

   Academic Institutions and Nonprofits only

6. pJT126 lenti pEF-rTetR(SE-G72P)-3XFLAG-LibCloneSite-T2A-mCherry-BSD-WPRE

   Plasmid

   #161926

   Purpose

   Lentiviral backbone for GoldenGate cloning effectors as a fusion with rTetR(SE-G72P)-3XFLAG

   Depositor

   Lacra Bintu, Michael Bassik

   Article

   Tycko et al Cell. 2020 Dec 9. pii: S0092-8674(20)315

   Insert

   rTetR(SE-G72P)
   

   Use

   Lentiviral and Synthetic Biology

   Tags

   3XFLAG

   Expression

   Mammalian

   Promoter

   pEF

   Available Since

   Feb. 10, 2021

   Availability

   Academic Institutions and Nonprofits only

   

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7. rat brain inositol 1,4,5-trisphosphate 3-kinase A (Clone C5, partial sequence from V66)

   Plasmid

   #122450

   Purpose

   Expression of inositol 1,4,5-trisphosphate 3-kinase A

   Depositor

   Christophe Erneux

   Article

   Takazawa et al Biochem J. 1990 Nov 15;272(1):107-12.

   Insert

   inositol 1,4,5-trisphosphate 3-kinase A (Itpka Rat)
   

   Tags

   beta-galactosidase

   Expression

   Bacterial

   Mutation

   coding sequence from V66 till stop codon

   Available Since

   March 12, 2019

   Availability

   Academic Institutions and Nonprofits only

   

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8. pFastBac StrepII msfGFP TEV cloning vector with BioBrick polycistronic restriction sites(11R-GFP)

   Plasmid

   #48297

   Depositor

   Scott Gradia

   Article

   Gradia et al Methods Enzymol. 2017;592:1-26. doi: 10.

   Type

   Empty backbone

   Tags

   StrepII and msfGFP

   Expression

   Insect

   Available Since

   Sept. 23, 2013

   Availability

   Academic Institutions and Nonprofits only

   

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9. pFastBac TEV SNAPf Prescission TwinStrepII cloning vector with BioBrick polycistronic restriction sites (11-SNAP-V3)

   Plasmid

   #55217

   Depositor

   Scott Gradia

   Article

   Gradia et al Methods Enzymol. 2017;592:1-26. doi: 10.

   Type

   Empty backbone

   Tags

   SNAPf and TwinStrepII

   Expression

   Insect

   Available Since

   July 9, 2014

   Availability

   Academic Institutions and Nonprofits only

   

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10. pFastBac TwinStrepII Prescission SNAPf TEV cloning vector with BioBrick polycistronic restriction sites (11-SNAP-V1)

    Plasmid

    #55216

    Depositor

    Scott Gradia

    Article

    Gradia et al Methods Enzymol. 2017;592:1-26. doi: 10.

    Type

    Empty backbone

    Tags

    SNAPf and TwinStrepII

    Expression

    Insect

    Available Since

    June 16, 2014

    Availability

    Academic Institutions and Nonprofits only

    

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11. CJW4617

    Bacterial Strain

    #177118

    Purpose

    E. coli strain carrying IPTG-inducible gfp-muNS gene for monitoring the mobility of cytoplasmic particles.

    Depositor

    Christine Jacobs-Wagner

    Article

    Parry et al Cell. 2014 Jan 16;156(1-2):183-94. doi:

    Bacterial Resistance

    Kanamycin

    Available Since

    Jan. 6, 2022

    Availability

    Academic Institutions and Nonprofits only

12. pCL040 lenti TRE-3xFLAG-LibCloneSite pEF-rTetR(SE-G72P)-VP48-T2A-mCherry-BSD-WPRE

    Plasmid

    #198054

    Purpose

    All-in-one lentiviral backbone for doxycycline-inducible expression of a 3xFLAG-tagged protein of interest, which can be inserted with Golden Gate cloning

    Depositor

    Lacra Bintu

    Article

    Ludwig et al Cell Syst. 2023 Jun 21;14(6):482-500.e8.

    Inserts

    rTetR(SE-G72P)-VP48-T2A-mCherry-BSD
    3xFLAG-insert
    

    Use

    Lentiviral and Synthetic Biology

    Tags

    3xFLAG

    Expression

    Mammalian

    Promoter

    TRE3G and pEF

    Available Since

    June 27, 2023

    Availability

    Academic Institutions and Nonprofits only

    

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13. UQ4884

    Bacterial Strain

    #37161

    Depositor

    Gary Roberts

    Article

    Kerby et al J Bacteriol. 2008 May;190(9):3336-43. Ep

    Bacterial Resistance

    Ampicillin

    Species

    Burkholderia xenovorans

    Available Since

    June 21, 2012

    Availability

    Academic Institutions and Nonprofits only

14. UQ4802

    Bacterial Strain

    #37160

    Depositor

    Gary Roberts

    Article

    Kerby et al J Bacteriol. 2008 May;190(9):3336-43. Ep

    Bacterial Resistance

    Ampicillin

    Species

    Burkholderia xenovorans

    Available Since

    June 21, 2012

    Availability

    Academic Institutions and Nonprofits only

15. TB204

    Bacterial Strain

    #230033

    Purpose

    Fluorescently labelled (monomeric sfGFP) E. coli

    Depositor

    Calin Guet, Tobias Bergmiller

    Article

    Tomasek et al J Biotechnol. 2018 Feb 20;268:40-52. doi

    Bacterial Resistance

    None

    Available Since

    Feb. 3, 2025

    Availability

    Academic Institutions and Nonprofits only

16. TB201

    Bacterial Strain

    #230031

    Purpose

    Fluorescently labelled (YFP) E. coli

    Depositor

    Calin Guet, Tobias Bergmiller

    Article

    Tomasek et al J Biotechnol. 2018 Feb 20;268:40-52. doi

    Bacterial Resistance

    None

    Available Since

    Feb. 3, 2025

    Availability

    Academic Institutions and Nonprofits only

17. TUNEYALI-TF Library

    Pooled Library

    #217744

    Purpose

    Regulating transcription factors in Yarrowia lipolytica

    Depositor

    Irina Borodina

    Expression

    Yeast

    Available Since

    May 20, 2025

    Availability

    Academic Institutions and Nonprofits only

18. SB39

    Bacterial Strain

    #235121

    Purpose

    E. coli strain suitable for cloning, expression, library construction (good electroporation yield) and quantitative bacterial two-hybrid (qB2H).

    Depositor

    Oscar Ramos

    Bacterial Resistance

    None

    Available Since

    Oct. 27, 2025

    Availability

    Academic Institutions and Nonprofits only

19. TB205

    Bacterial Strain

    #230034

    Purpose

    Fluorescently labelled (mCherry) E. coli

    Depositor

    Calin Guet, Tobias Bergmiller

    Article

    Bergmiller et al Science. 2017 Apr 21;356(6335):311-315.

    Bacterial Resistance

    None

    Available Since

    Feb. 3, 2025

    Availability

    Academic Institutions and Nonprofits only

20. sgRNA pool for Streptococcus pneumoniae

    Pooled Library

    #170432

    Purpose

    CRISPR library targeting S. pneumoniae D39V

    Depositor

    Jan-Willem Veening

    Article

    Liu et al Cell Host Microbe. 2021 Jan 13;29(1):107

    Expression

    Bacterial

    Use

    CRISPR

    Available Since

    Aug. 31, 2021

    Availability

    Academic Institutions and Nonprofits only