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We narrowed to 61,187 results for: cloning

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Showing: 281 - 300 of 61187 results

  1. pCL040 lenti TRE-3xFLAG-LibCloneSite pEF-rTetR(SE-G72P)-VP48-T2A-mCherry-BSD-WPRE

    Plasmid
    #198054
    Purpose
    All-in-one lentiviral backbone for doxycycline-inducible expression of a 3xFLAG-tagged protein of interest, which can be inserted with Golden Gate cloning
    Depositor
    Lacra Bintu
    Article
    Ludwig et al Cell Syst. 2023 Jun 21;14(6):482-500.e8.
    Inserts
    rTetR(SE-G72P)-VP48-T2A-mCherry-BSD
    3xFLAG-insert
    Use
    Lentiviral and Synthetic Biology
    Tags
    3xFLAG
    Expression
    Mammalian
    Promoter
    TRE3G and pEF
    Available Since
    June 27, 2023
    Availability
    Academic Institutions and Nonprofits only
    198054-plasmid-map-sequence-id-391373

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  4. TUNEYALI-TF Library

    Pooled Library
    #217744
    Purpose
    Targets 56 transcription factors in Yarrowia lipolytica, enabling the knockout or modulation of expression levels for these factors.
    Depositor
    Irina Borodina
    Expression
    Yeast
    Available Since
    May 20, 2025
    Availability
    Academic Institutions and Nonprofits only

  8. SB39

    Bacterial Strain
    #235121
    Purpose
    E. coli strain suitable for cloning, expression, library construction (good electroporation yield) and quantitative bacterial two-hybrid (qB2H).
    Depositor
    Oscar Ramos
    Bacterial Resistance
    None
    Available Since
    Oct. 27, 2025
    Availability
    Academic Institutions and Nonprofits only

  10. FD1

    Bacterial Strain
    #125258
    Purpose
    Derivative of MG1655 used for the screening of clones able to survive the bad seed toxicity of dCas9, while still efficiently silencing the expression of mcherry
    Depositor
    David Bikard
    Article
    Depardieu et al Methods. 2019 Aug 1. pii: S1046-2023(18)
    Bacterial Resistance
    None
    Available Since
    Aug. 23, 2019
    Availability
    Academic Institutions and Nonprofits only

  11. FX cloning Kit

    Plasmid Kit
    #1000000039
    Purpose
    Use fragment exchange (FX) cloning to generate constructs for screening protein expression in several organisms with a variety of N- and C-terminal tags.
    Depositor
    Eric Geertsma
    Application
    Cloning and Synthetic Biology, Gene Expression and Labeling
    Vector Type
    Bacterial Expression, Insect Expression, Mammalian Expression, Xenopus Expression, Yeast Expression
    Cloning Type
    FX Cloning
    Available Since
    April 2, 2014
    Availability
    Academic Institutions and Nonprofits only

  12. zCas9i cloning kit: MoClo-compatible CRISPR/Cas9 cloning kit with intronized Cas9

    Plasmid Kit
    #1000000171
    Purpose
    MoClo-compatible vectors for assembly of CRISPR/Cas9 constructs using a high efficiency intron-optimized SpCas9-coding gene in plants.
    Depositor
    Sylvestre Marillonnet, Johannes Stuttmann
    Application
    Cloning and Synthetic Biology, Genome Editing
    Vector Type
    Plant Expression
    Editing Type
    CRISPR
    Cloning Type
    Golden Gate (MoClo)
    Available Since
    March 25, 2021
    Availability
    Academic Institutions and Nonprofits only

  14. RAS mutant clone collection

    Plasmid Kit
    #1000000089
    Purpose
    Gateway entry clones containing expression vectors for RAS protein mutants for in vitro and in vivo research.
    Depositor
    Dominic Esposito
    Application
    Gene Expression and Labeling
    Vector Type
    Mammalian Expression
    Available Since
    Dec. 1, 2016
    Availability
    Academic Institutions and Nonprofits only

  15. pMVP and pMAGIC Cloning Systems

    Plasmid Kit
    #1000000155
    Purpose
    For rapid modular cloning of adenoviral, lentiviral, PiggyBac and Sleeping Beauty transposon vectors for transgene or RNAi delivery (pMVP) and dCas9-based epigenetic engineering (pMAGIC).
    Depositor
    Christopher Newgard
    Application
    Cloning and Synthetic Biology, Genome Editing
    Vector Type
    Adenoviral, Lentiviral, Mammalian Expression
    Editing Type
    CRISPR
    Cloning Type
    MultiSite Gateway
    Available Since
    Sept. 6, 2019
    Availability
    Academic Institutions and Nonprofits only

  16. Golden GATEway Cloning Kit

    Plasmid Kit
    #1000000054
    Purpose
    A collection that combines Golden Gate with Multisite Gateway cloning for assembly of complex fusion proteins or transgenesis constructs.
    Depositor
    Joachim Wittbrodt
    Application
    Cloning and Synthetic Biology
    Vector Type
    Bacterial Expression, Eukaryotic Expression, Mammalian Expression, Zebrafish Expression
    Cloning Type
    Golden Gate
    Available Since
    Sept. 10, 2014
    Availability
    Academic Institutions and Nonprofits only

  17. 4G-Cloning Plasmid Kit

    Plasmid Kit
    #1000000252
    Purpose
    Use 4G (Golden-Gate-guided Gibson) cloning for rapid assembly of single- or multi-gene expression vectors for multi-subunit protein complexes in various expression hosts.
    Depositor
    Stephan Gruber
    Application
    Cloning and Synthetic Biology
    Vector Type
    Bacterial Expression
    Cloning Type
    Golden Gate
    Available Since
    Jan. 21, 2025
    Availability
    Academic Institutions and Nonprofits only

  18. FNL Combinatorial Cloning Platform

    Plasmid Kit
    #1000000211
    Purpose
    A Frederick National Laboratory (FNL) combinatorial cloning set to generate many tagged expression clones in parallel for protein production, in vivo gene expression, and shRNA and CRISPR delivery.
    Depositor
    Dominic Esposito
    Application
    Cloning and Synthetic Biology, Gene Expression and Labeling
    Vector Type
    Bacterial Expression, Insect Expression, Mammalian Expression
    Cloning Type
    MultiSite Gateway
    Available Since
    Dec. 22, 2022
    Availability
    Academic Institutions and Nonprofits only

  19. CAG-MCS-WPRE-SV40 - GCH1

    Plasmid
    #230814
    Purpose
    AAV cloning vector for expression under the CAG promoter.
    Depositor
    Kimberly Ritola
    Type
    Empty backbone
    Use
    AAV; Cloning vector
    Available Since
    March 26, 2025
    Availability
    Academic Institutions and Nonprofits only
    230814-plasmid-map-sequence-id-466629

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  20. EF1a-MCS-WPRE-SV40 - GCH13

    Plasmid
    #230820
    Purpose
    AAV cloning vector for expression under the EF1a promoter.
    Depositor
    Kimberly Ritola
    Type
    Empty backbone
    Use
    AAV; Cloning vector
    Available Since
    Jan. 6, 2025
    Availability
    Academic Institutions and Nonprofits only
    230820-plasmid-map-sequence-id-457137

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Showing: 281 - 300 of 61187 results