We narrowed to 9,990 results for: SUB;

1. pCHC005 (∆RE)

   Plasmid

   #226199

   Purpose

   Conjugation knockout plasmid (pK18mobsacB) to delete H16_0006 (Type I restriction endonuclease subunit).

   Depositor

   Christopher Johnson

   Article

   Calvey et al Metab Eng. 2023 Jan;75:78-90. doi: 10.10

   Insert

   Homology arms for type I restriction-modification system restriction subunit H16_A0006
   

   Use

   Synthetic Biology

   Available Since

   Nov. 18, 2024

   Availability

   Industry, Academic Institutions, and Nonprofits

   

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2. pFA6a-GFP (F64L, S65T, V163A)-TRP1

   Plasmid

   #53184

   Purpose

   chromosomal tagging with stable GFP

   Depositor

   John Pringle

   Article

   Ko et al Mol Biol Cell. 2007 Dec;18(12):5139-53.

   Insert

   TRP1 (TRP1 Budding Yeast)
   

   Use

   Yeast genomic targeting

   Tags

   GFP (F64L, S65T, V163A)

   Available Since

   May 27, 2014

   Availability

   Academic Institutions and Nonprofits only

3. pQE60 R124K wgMDH

   Plasmid

   #204258

   Purpose

   Bacterial expression of R124K wgMDH with IPTG induction - Active site mutant

   Depositor

   Malate dehydrogenase CUREs Community (MCC)

   Article

   Cox et al FEBS J. 2005 Feb;272(3):643-54. doi: 10.

   Insert

   MDHG_CITLA
   

   Tags

   6X His

   Expression

   Bacterial

   Mutation

   R124K

   Available Since

   Oct. 23, 2023

   Availability

   Academic Institutions and Nonprofits only

   

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4. pQE60 R124Q wgMDH

   Plasmid

   #204259

   Purpose

   Bacterial expression of R124Q wgMDH with IPTG induction - Active site mutant

   Depositor

   Malate dehydrogenase CUREs Community (MCC)

   Article

   Cox et al FEBS J. 2005 Feb;272(3):643-54. doi: 10.

   Insert

   MDHG_CITLA
   

   Tags

   6X His

   Expression

   Bacterial

   Mutation

   R124Q

   Available Since

   Oct. 23, 2023

   Availability

   Academic Institutions and Nonprofits only

   

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5. pQE60 R130E wgMDH

   Plasmid

   #204267

   Purpose

   Bacterial expression of R130E wgMDH with IPTG induction - Active site mutant

   Depositor

   Malate dehydrogenase CUREs Community (MCC)

   Article

   Cox et al FEBS J. 2005 Feb;272(3):643-54. doi: 10.

   Insert

   MDHG_CITLA
   

   Tags

   6X His

   Expression

   Bacterial

   Mutation

   R130E

   Available Since

   Oct. 23, 2023

   Availability

   Academic Institutions and Nonprofits only

   

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6. pCX-Lyn-SpiCee

   Plasmid

   #140837

   Purpose

   mammalian expression of Lyn-SpiCee, a lipid raft-targeted variant of the calcium buffer SpiCee that prevents downstream signaling

   Depositor

   Xavier Nicol

   Article

   Ros et al Cell Rep. 2020 Jul 21;32(3):107934. doi:

   Insert

   Lyn-SpiCee
   

   Tags

   mRFP

   Expression

   Mammalian

   Promoter

   CAG

   Available Since

   July 24, 2020

   Availability

   Academic Institutions and Nonprofits only

   

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7. pCX-SpiCee-Kras

   Plasmid

   #140838

   Purpose

   mammalian expression of SpiCee-Kras, a plasma membrane-targeted but lipid raft-excluded variant of the calcium buffer SpiCee that prevents downstream signaling

   Depositor

   Xavier Nicol

   Article

   Ros et al Cell Rep. 2020 Jul 21;32(3):107934. doi:

   Insert

   SpiCee-Kras
   

   Tags

   mRFP

   Expression

   Mammalian

   Promoter

   CAG

   Available Since

   July 24, 2020

   Availability

   Academic Institutions and Nonprofits only

   

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8. SAC1delta452-587-FKBP-mCherry

   Plasmid

   #108131

   Purpose

   Recruitable inactive SAC1 (linker deletion)

   Depositor

   Gerry Hammond

   Article

   Zewe et al Elife. 2018 Feb 20;7. pii: 35588. doi: 1

   Insert

   SACM1L(1-451):FKBP1A(3-108):mCherry (SACM1L Human)
   

   Expression

   Mammalian

   Mutation

   SACM1L(1-451)

   Promoter

   CMV

   Available Since

   April 6, 2018

   Availability

   Academic Institutions and Nonprofits only

   

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9. pHis-hMTMR1

   Plasmid

   #100723

   Purpose

   Expression of human MTMR1 PH-PTP domain

   Depositor

   Byung Il Lee

   Article

   Bong et al PLoS One. 2016 Mar 28;11(3):e0152611. do

   Insert

   MTMR1 (MTMR1 Human)
   

   Tags

   His

   Expression

   Bacterial

   Mutation

   Contains amino acids 95-607

   Promoter

   T7

   Available Since

   Nov. 9, 2017

   Availability

   Academic Institutions and Nonprofits only

   

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10. pEYFP-N1-eKR2

    Plasmid

    #115337

    Purpose

    Expresses enhanced sodium pump for light-driven extrusion of sodium; activation with green light; codon-optimized for human/mouse

    Depositor

    Peter Hegemann

    Article

    Grimm et al Sci Rep. 2018 Jun 18;8(1):9316. doi: 10.

    Insert

    eKR2
    

    Tags

    EYFP

    Expression

    Mammalian

    Mutation

    none

    Promoter

    CMV

    Available Since

    Sept. 13, 2018

    Availability

    Academic Institutions and Nonprofits only

    

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11. pHAGE DN Cullin1

    Plasmid

    #41911

    Depositor

    Stephen Elledge

    Article

    Emanuele et al Cell. 2011 Oct 14;147(2):459-74. doi: 10

    Insert

    CUL1 (CUL1 Human)
    

    Use

    Lentiviral

    Promoter

    CMV

    Available Since

    April 17, 2013

    Availability

    Academic Institutions and Nonprofits only

    

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12. pEGFP-ATXN1-30Q

    Plasmid

    #33238

    Depositor

    Huda Zoghbi

    Article

    Cummings et al Nat Genet. 1998 Jun;19(2):148-54.

    Insert

    Ataxin-1 (ATXN1 Human)
    

    Tags

    GFP

    Expression

    Mammalian

    Mutation

    insert contains a stretch of 32 amino acids (30 Q…

    Available Since

    July 18, 2014

    Availability

    Academic Institutions and Nonprofits only

    

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13. pyc111f

    Plasmid

    #158780

    Purpose

    ribosome-tagged, Cre-dependent GCaMP for soma localization

    Depositor

    Jennifer Garrison, Zachary Knight

    Article

    Chen et al Neuron. 2020 Jun 5. pii: S0896-6273(20)3

    Insert

    GCaMP6f-RPL10a
    

    Use

    AAV

    Available Since

    Sept. 28, 2020

    Availability

    Academic Institutions and Nonprofits only

    

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14. pLENTI_RPS3

    Plasmid

    #127128

    Depositor

    Thomas Tuschl

    Article

    Meyer et al Mol Cell. 2020 Mar 19;77(6):1193-1205.e5

    Insert

    RPS3 (RPS3 Human)
    

    Use

    Lentiviral

    Available Since

    July 26, 2019

    Availability

    Academic Institutions and Nonprofits only

    

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15. pAAV-EF1a-DIO-H2B.APEX-P2A-EGFP

    Plasmid

    #182825

    Purpose

    Cre-dependent expression of H2B-APEX fusion

    Depositor

    Yevgenia Kozorovitskiy

    Article

    Dumrongprechachan et al Nat Commun. 2021 Aug 11;12(1):4855. doi:

    Insert

    H2B.APEX-P2A-EGFP
    

    Use

    AAV

    Tags

    EGFP reporter (P2A)

    Promoter

    EF1a

    Available Since

    May 16, 2022

    Availability

    Academic Institutions and Nonprofits only

    

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16. pet28a-His6-Halo-Tev-Keap1

    Plasmid

    #62456

    Purpose

    His6-halo tagged human Keap1 protein for E.coli expression

    Depositor

    Yimon Aye

    Article

    Parvez et al J Am Chem Soc. 2014 Dec 31.

    Insert

    Keap1 (KEAP1 Human)
    

    Tags

    His-Halo-Tev

    Expression

    Bacterial

    Available Since

    March 22, 2017

    Availability

    Academic Institutions and Nonprofits only

    

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17. pBJ1 neo-hu FceRI gamma

    Plasmid

    #16540

    Depositor

    Jean Kinet

    Article

    Kuster et al J Biol Chem 1990 Apr 15;265(11):6448-52.

    Insert

    FCER1G (FCER1G Human)
    

    Expression

    Mammalian

    Available Since

    Jan. 10, 2008

    Availability

    Academic Institutions and Nonprofits only

    

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18. pSRalpha-MSV-mouse Cdk4wt-Tk-neo

    Plasmid

    #115525

    Purpose

    Mammalian expression construct

    Depositor

    Martine Roussel

    Article

    Matsushime et al Cell. 1992 Oct 16;71(2):323-34.

    Insert

    Mouse Cdk4 (Cdk4 Mouse)
    

    Use

    Retroviral

    Available Since

    Oct. 3, 2018

    Availability

    Academic Institutions and Nonprofits only

19. AAV-GFAP(long)-FLEX-GCaMP7f

    Plasmid

    #189612

    Purpose

    Cre-dependent GCaMP7f under the GFAP promoter

    Depositor

    Martin Riccomagno

    Article

    Agnew-Svoboda et al Cell Rep Methods. 2022 Aug 22;2(8):10027

    Insert

    GCaMP7f
    

    Use

    AAV and Cre/Lox

    Tags

    GCaMP7f

    Expression

    Mammalian

    Available Since

    Sept. 16, 2022

    Availability

    Academic Institutions and Nonprofits only

    

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20. KG#901

    Plasmid

    #110936

    Purpose

    Expresses Emerald-RAB-3 in the cholinergic motor neuron DA9 for imaging synaptic vesicles in a single neuron in a living animal

    Depositor

    Kenneth Miller

    Article

    Edwards et al Genetics. 2018 Nov;210(3):947-968. doi:

    Inserts

    mig-13 promoter
    Emerald-RAB-3
    unc-54 3' control region with 1 artificial intron just upstream and 1 artificial intron in control region
    

    Expression

    Bacterial

    Available Since

    March 11, 2021

    Availability

    Academic Institutions and Nonprofits only

    

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