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pAAV-CaMKIIa-GCaMP 6m-p2A-ChRmine-Kv2.1-WPRE

Plasmid

#131003

Purpose

bicistronic AAV vector to express GCaMP6m and soma-targeted ChRmine under the control of CamKIIa promoter

Depositor

Karl Deisseroth

Article

Marshel et al Science. 2019 Aug 9;365(6453). pii: scie

Insert

ChRmine

Use

AAV

Tags

Kv2.1-HA

Promoter

CaMKIIa

Available Since

Sept. 9, 2019

Availability

Academic Institutions and Nonprofits only

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pAAV-CaMKIIa-ChRmine-mScarlet-Kv2.1-WPRE

Plasmid

#130991

Purpose

AAV vector to drive the expression of soma-targeted ChRmine-mScarlet under the control of CamKIIa promoter

Depositor

Karl Deisseroth

Article

Marshel et al Science. 2019 Aug 9;365(6453). pii: scie

Has Service

AAV8

Insert

ChRmine

Use

AAV

Tags

mScarlet-Kv2.1

Promoter

CaMKIIa

Available Since

Sept. 9, 2019

Availability

Academic Institutions and Nonprofits only

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pcDNA4/TO-HRASG12V

Plasmid

#216509

Purpose

Expresses HRAS with a G12V point mutation in human cells under a tetracycline/doxycycline-inducible promoter

Depositor

John Diffley

Article

Zeng et al Cell. 2023 Feb 2;186(3):528-542.e14. doi

Insert

HRAS (HRAS Human)

Tags

HA

Expression

Mammalian

Mutation

G12V

Available Since

April 12, 2024

Availability

Academic Institutions and Nonprofits only

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pCdOpt-BMX

Plasmid

#203929

Purpose

This plasmid contains a codon optimized BleMX drug resistance cassette for generating gene deletions in C. glabrata, C. auris, C. albicans or S. cerevisiae

Depositor

Scott Briggs

Article

Gregor et al mSphere. 2023 Nov 6:e0031123. doi: 10.11

Insert

BleMX

Expression

Yeast

Mutation

BleMX was codon optimized for Candida albicans or…

Available Since

Dec. 18, 2023

Availability

Academic Institutions and Nonprofits only

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CMV-10-3xFLAG-LGP2

Plasmid

#58681

Purpose

Expresses 3x Flag-tagged LGP2 in mammalian cells

Depositor

Curt Horvath

Article

Bamming et al J Biol Chem. 2009 Apr 10;284(15):9700-12

Insert

laboratory of genetics and physiology 2 (DHX58 Human)

Tags

3x FLAG

Expression

Mammalian

Promoter

CMV

Available Since

July 31, 2014

Availability

Academic Institutions and Nonprofits only

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pcDNA3.3-3XFLAG-AGO2

Plasmid

#136687

Purpose

Expresses human AGO2 with an N-terminal 3XFLAG tag in mammalian cells (using a CMV promoter)

Depositor

David Bartel

Article

McGeary et al Science. 2019 Dec 20;366(6472). pii: sci

Insert

argonaute RISC Catalytic Component 2 (AGO2 Human)

Tags

3XFLAG

Expression

Mammalian

Available Since

March 4, 2020

Availability

Academic Institutions and Nonprofits only

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pAAV-CAG-ArchT-GFP

Plasmid

#29777

Purpose

AAV-mediated expression of ArchT-GFP for neuronal inhibition

Depositor

Edward Boyden

Article

Han et al Front Syst Neurosci. 2011;5:18. Epub 201

Insert

ArchT-GFP

Use

AAV; Adeno-associated virus

Tags

GFP

Expression

Mammalian

Mutation

N/A

Promoter

CAG

Available Since

July 7, 2011

Availability

Academic Institutions and Nonprofits only

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pEF-Bos-FLAG RIG-I MIII

Plasmid

#52877

Purpose

Expresses RIG-I with a mutation to motif III in mammalian cells

Depositor

Curt Horvath

Article

Bamming et al J Biol Chem. 2009 Apr 10;284(15):9700-12

Insert

DDX58 (RIGI Human)

Tags

FLAG

Expression

Mammalian

Mutation

T409A, S411A

Available Since

May 9, 2014

Availability

Academic Institutions and Nonprofits only

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pAAV-UBC-ERT2-iCre-ERT2

Plasmid

#178060

Purpose

Conditionally active form of Cre recombinase (activated in response to tamoxifen) under UBC promoter.

Depositor

Edward Boyden

Article

Linghu et al Nat Biotechnol. 2023 Jan 2. doi: 10.1038

Insert

ERT2-iCre-ERT2

Use

AAV

Expression

Mammalian

Promoter

Human ubiquitin C (UBC) promoter

Available Since

Oct. 9, 2022

Availability

Academic Institutions and Nonprofits only

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pAAV-CaMKIIa-ChRmine-mScarlet-WPRE

Plasmid

#130990

Purpose

AAV vector to drive the expression of ChRmine-mScarlet under the control of CamKIIa promoter

Depositor

Karl Deisseroth

Article

Marshel et al Science. 2019 Aug 9;365(6453). pii: scie

Insert

ChRmine

Use

AAV

Tags

mScarlet

Promoter

CaMKIIa

Available Since

Sept. 9, 2019

Availability

Academic Institutions and Nonprofits only

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pF152 pcDNA3.1(+)SOD1A4V

Plasmid

#26398

Depositor

Elizabeth Fisher

Article

Stevens et al PLoS One. 2010 . 5(3):e9541.

Insert

SOD1 (SOD1 Human)

Expression

Mammalian

Mutation

A4V

Available Since

Nov. 23, 2010

Availability

Academic Institutions and Nonprofits only
pLKO.1-puro_CMV_NES-Caprola_on-mEGFP

Plasmid

#194694

Purpose

CMV driven expression of the consitutively active calcium recorder positive control Caprola_on fused to mEGFP for neuronal expression through lentivirus transduction

Depositor

Kai Johnsson

Article

Huppertz et al Science. 2024 Feb 23;383(6685):890-897.

Insert

Caprola_on-mEGFP

Use

Lentiviral

Tags

mEGFP

Promoter

CMV

Available Since

Feb. 23, 2024

Availability

Academic Institutions and Nonprofits only

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pCDFDuet-nsp7-nsp8

Plasmid

#159092

Purpose

Coexpression construct of Nsp7 with an N-terminal His-tag and Nsp8

Depositor

Seth Darst

Article

Chen et al Cell. 2020 Sep 17;182(6):1560-1573.e13.

Inserts

NSP7
NSP8

Tags

His

Expression

Bacterial

Promoter

T7

Available Since

Sept. 28, 2020

Availability

Academic Institutions and Nonprofits only

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pAAV-CaMKIIa-ChRmine-eYFP-WPRE

Plasmid

#130988

Purpose

AAV vector to drive the expression of ChRmine-eYFP under the control of CamKIIa promoter

Depositor

Karl Deisseroth

Article

Marshel et al Science. 2019 Aug 9;365(6453). pii: scie

Insert

ChRmine

Use

AAV

Tags

eYFP

Promoter

CaMKIIa

Available Since

Sept. 9, 2019

Availability

Academic Institutions and Nonprofits only

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pEF-Bos-FLAG MDA5 MI

Plasmid

#52875

Purpose

Expresses MDA5 with a mutation to motif I in mammalian cells

Depositor

Curt Horvath

Article

Bamming et al J Biol Chem. 2009 Apr 10;284(15):9700-12

Insert

IFIH1 (IFIH1 Human)

Tags

FLAG

Expression

Mammalian

Mutation

K335A, E658K

Available Since

May 9, 2014

Availability

Academic Institutions and Nonprofits only

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MSCV N-term HA-FLAG puro BRD7

Plasmid

#41934

Depositor

Stephen Elledge

Article

Burrows et al Proc Natl Acad Sci U S A. 2010 Aug 10;10

Insert

BRD7 (BRD7 Human)

Use

Retroviral

Tags

HA-Flag

Expression

Mammalian

Available Since

April 26, 2013

Availability

Academic Institutions and Nonprofits only
PX459-TP53-exon4

Plasmid

#217455

Purpose

For TP53 knockout targeting exon 4 of TP53

Depositor

John Diffley

Article

Zeng et al Cell. 2023 Feb 2;186(3):528-542.e14. doi

Insert

CRISPR Cas9 guide for TP53 (TP53 Human)

Use

CRISPR

Expression

Mammalian

Available Since

April 12, 2024

Availability

Academic Institutions and Nonprofits only

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pAAV-FLEX-ArchT-GFP

Plasmid

#28307

Purpose

Cre-dependent viral expression of ArchT-GFP for neuronal inhibition

Depositor

Edward Boyden

Article

Han et al Front Syst Neurosci. 2011;5:18. Epub 201

Insert

ArchT-GFP

Use

AAV and Cre/Lox; Adeno-associated virus

Tags

GFP

Expression

Mammalian

Mutation

N/A

Promoter

CAG

Available Since

July 7, 2011

Availability

Academic Institutions and Nonprofits only

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PX459-TP53-exon5

Plasmid

#217456

Purpose

For TP53 knockout targeting exon 5 of TP53

Depositor

John Diffley

Article

Zeng et al Cell. 2023 Feb 2;186(3):528-542.e14. doi

Insert

CRISPR Cas9 guide for TP53 (TP53 Human)

Use

CRISPR

Expression

Mammalian

Available Since

April 12, 2024

Availability

Academic Institutions and Nonprofits only

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pMis1_1B

Plasmid

#192079

Purpose

A pBBR-1 based vector with IncP group plasmid compatibility for expression in Methylorubrum extorquens using rhamnose dependent induction

Depositor

Markus Buchhaupt

Article

Poschel et al Microbiologyopen. 2022 Oct;11(5):e1325.

Inserts

KanR
rhaR
rhaS
pBBR1 rep

Expression

Bacterial

Available Since

Dec. 15, 2022

Availability

Academic Institutions and Nonprofits only

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