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Enter a sequence to perform a BLAST-based search of Addgene plasmid sequence databases.

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We narrowed to 9,229 results for: sgRNA

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Showing: 3061 - 3080 of 9229 results

  1. pFH73

    Plasmid
    #128222
    Purpose
    classic sgRNA backbone for tRNA-gRNA array, Position 1, combine with OsU6-2 promoter module (pFH36)
    Depositor
    Vladimir Nekrasov
    Publication
    Hahn et al BMC Plant Biol. 2020 Apr 23;20(1):179. d
    Insert
    classic sgRNA backbone for tRNA-gRNA array, Position 1, combine with OsU6-2 promoter module (pFH36)
    Use
    CRISPR and Synthetic Biology
    Expression
    Plant
    Available since
    Aug. 19, 2019
    Availability
    Academic Institutions and Nonprofits only
    128222-plasmid-map-sequence-id-252874

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  2. eSpCas9-hGeminin

    Plasmid
    #199344
    Purpose
    modified version of the eSpCas9(1.1)_No_FLAG_ATP1A1_G3_Dual_sgRNA plasmid (addgene #86613) where the C-terminus of the eSpCas9 enzyme was fused to amino acids 1 – 110 of human Geminin
    Depositor
    Iain Hagan
    Publication
    Morrison et al Open Biol. 2023 Apr;13(4):230021. doi: 1
    Has service
    50 µg Transfection-Ready DNA and 100 µg Transfection-Ready DNA
    Insert
    ATP1A1 G3 sgRNA+user-specified sgRNA+enhanced specificity Cas9 (1.1) (addgene 86613) with cas9 fused to hgem fragment 1-110) (GMNN S. pyogenes)
    Tags
    cas9 c-term fused to hgemenin 1-110 fragment
    Expression
    Mammalian
    Mutation
    cas9 c-term fused to hgemenin 1-110 fragment
    Promoter
    cbh
    Available since
    Dec. 13, 2024
    Availability
    Academic Institutions and Nonprofits only
    Cited by
    199344-plasmid-map-sequence-id-402934

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  5. pcDNA3.1-CRISPR-Puro-sgRPL3-62

    Plasmid
    #208647
    Purpose
    pcDNA3.1 based plasmid for transient transfection of sgRNA-cas9. Containing sgRNA targeting human RPL3 (GRCh38.p14_Chr22:39312968-39312987), 62 is a number given by www.e-crisp.org.
    Depositor
    Quanfu Ma
    Insert
    sgRNA targeting human RPL3 (ENST00000216146) C-terminal, No.62 (RPL3 Human, Synthetic)
    Use
    CRISPR
    Expression
    Mammalian
    Promoter
    CMV and U6 in tandem
    Available since
    Jan. 9, 2024
    Availability
    Academic Institutions and Nonprofits only
    208647-plasmid-map-sequence-id-418374

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  6. pEPQD0KN0751

    Plasmid
    #185628
    Purpose
    Modified tobacco rattle virus RNA2 acceptor vector that could be used for expression of sgRNAs, contains restriction enzyme sites for golden gate assembly using BsaI
    Depositor
    Nicola Patron
    Publication
    Dudley et al Commun Biol. 2022 Sep 10;5(1):949. doi:
    Has service
    50 µg Transfection-Ready DNA and 100 µg Transfection-Ready DNA
    Insert
    lacZ
    Use
    CRISPR and Synthetic Biology
    Available since
    Aug. 4, 2022
    Availability
    Academic Institutions and Nonprofits only
    185628-plasmid-map-sequence-id-368556

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  14. pH-STEME-NG-esgRNA

    Plasmid
    #138136
    Purpose
    Targeted simultaneous C-to-T and A-to-G in rice
    Depositor
    Caixia Gao
    Insert
    APOBEC3A-wtTadA-TadA7.10-nCas9-NG-UGI-NLS
    Expression
    Plant
    Available since
    Sept. 23, 2020
    Availability
    Academic Institutions and Nonprofits only
    138136-plasmid-map-sequence-id-311723

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  20. pDIV313

    Plasmid
    #204956
    Purpose
    AMA1 plasmid with Aspergillus optimized Mad7, hph (hygromycin) resistance marker and sgRNA targeting albA locus in A. niger
    Depositor
    Uffe Mortensen
    Publication
    Vanegas et al J Fungi (Basel). 2022 Dec 22;9(1):16. do
    Inserts
    Mad7
    hph (hygromycin resistance marker)
    sgRNA (CAGCAATGCTTCCATGCAATT) targeting albA locus in A. niger flanked by a tRNA-Gly repeat
    Use
    CRISPR; Fungal expression
    Tags
    SV40 NLS
    Expression
    Bacterial
    Promoter
    Aspergillus fumigatus U3 promoter, Aspergillus ni…
    Available since
    Nov. 21, 2023
    Availability
    Academic Institutions and Nonprofits only
    Cited by
    204956-plasmid-map-sequence-id-415598

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Showing: 3061 - 3080 of 9229 results