We narrowed to 12,226 results for: sgRNA

1. AP54-5

   Plasmid

   #65599

   Purpose

   co-expression of Cas9 and a sgRNA targeting 3'end of C.elegans fbf-2 gene

   Depositor

   Geraldine Seydoux

   Article

   Paix et al Genetics. 2014 Dec;198(4):1347-56. doi:

   Insert

   sgRNA for APs12
   

   Use

   CRISPR

   Expression

   Worm

   Promoter

   U6

   Available Since

   June 19, 2015

   Availability

   Academic Institutions and Nonprofits only

2. AP56-6

   Plasmid

   #65593

   Purpose

   co-expression of Cas9 and a sgRNA targeting 3'end of C.elegans fbf-2 gene

   Depositor

   Geraldine Seydoux

   Article

   Paix et al Genetics. 2014 Dec;198(4):1347-56. doi:

   Insert

   sgRNA for APa13
   

   Use

   CRISPR

   Expression

   Worm

   Promoter

   U6

   Available Since

   June 9, 2015

   Availability

   Academic Institutions and Nonprofits only

3. AP56-5

   Plasmid

   #65591

   Purpose

   co-expression of Cas9 and a sgRNA targeting 3'end of C.elegans fbf-2 gene

   Depositor

   Geraldine Seydoux

   Article

   Paix et al Genetics. 2014 Dec;198(4):1347-56. doi:

   Insert

   sgRNA for APa13
   

   Use

   CRISPR

   Expression

   Worm

   Promoter

   U6

   Available Since

   June 9, 2015

   Availability

   Academic Institutions and Nonprofits only

4. AP56-8

   Plasmid

   #65596

   Purpose

   co-expression of Cas9 and a sgRNA targeting 3'end of C.elegans fbf-2 gene

   Depositor

   Geraldine Seydoux

   Article

   Paix et al Genetics. 2014 Dec;198(4):1347-56. doi:

   Insert

   sgRNA for APa13
   

   Use

   CRISPR

   Expression

   Worm

   Promoter

   U6

   Available Since

   June 9, 2015

   Availability

   Academic Institutions and Nonprofits only

5. AP56-7

   Plasmid

   #65594

   Purpose

   co-expression of Cas9 and a sgRNA targeting 3'end of C.elegans fbf-2 gene

   Depositor

   Geraldine Seydoux

   Article

   Paix et al Genetics. 2014 Dec;198(4):1347-56. doi:

   Insert

   sgRNA for APa13
   

   Use

   CRISPR

   Expression

   Worm

   Promoter

   U6

   Available Since

   June 9, 2015

   Availability

   Academic Institutions and Nonprofits only

6. AP54-3

   Plasmid

   #65597

   Purpose

   co-expression of Cas9 and a sgRNA targeting 3'end of C.elegans fbf-2 gene

   Depositor

   Geraldine Seydoux

   Article

   Paix et al Genetics. 2014 Dec;198(4):1347-56. doi:

   Insert

   sgRNA for APs12
   

   Use

   CRISPR

   Expression

   Worm

   Promoter

   U6

   Available Since

   June 9, 2015

   Availability

   Academic Institutions and Nonprofits only

7. sgOpti

   Plasmid

   #85681

   Purpose

   Lentiviral vector to express sgRNAs. The cloning site is BsmBI.

   Depositor

   Eric Lander, David Sabatini

   Article

   Fulco et al Science. 2016 Sep 29. pii: aag2445.

   Type

   Empty backbone

   Use

   CRISPR and Lentiviral

   Expression

   Mammalian

   Promoter

   hU6

   Available Since

   Feb. 1, 2017

   Availability

   Academic Institutions and Nonprofits only

   

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8. BPK2660

   Plasmid

   #70709

   Purpose

   Human expression plasmid for SaCas9 sgRNA(84nt in length) (need to clone in spacer into BsmBI sites): U6-BsmBIcassette-Sa-sgRNA(84)

   Depositor

   Keith Joung

   Article

   Kleinstiver et al Nat Biotechnol. 2015 Nov 2. doi: 10.1038

   Type

   Empty backbone

   Use

   CRISPR

   Expression

   Mammalian

   Promoter

   U6

   Available Since

   Nov. 6, 2015

   Availability

   Academic Institutions and Nonprofits only

   

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9. pRH2521

   Plasmid

   #84380

   Purpose

   Expression of sgRNA from a imyc promoter (Pimyc)

   Depositor

   Robert Husson

   Article

   Singh et al Nucleic Acids Res. 2016 Jul 12. pii: gkw

   Insert

   sgRNA cloning site
   

   Use

   CRISPR

   Expression

   Bacterial

   Available Since

   Jan. 27, 2017

   Availability

   Academic Institutions and Nonprofits only

   

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10. gRNA Cloning Vector Bbs I ver. 2

    Plasmid

    #85586

    Purpose

    An empty sgRNA expression vector. sgRNA sequence can be cloned into its Bbs I site. Its gRNA scaffold has efficient ver. 2 structure.

    Depositor

    Hodaka Fujii

    Article

    Fujita et al Sci Rep. 2016 Jul 28;6:30485. doi: 10.10

    Type

    Empty backbone

    Use

    CRISPR

    Expression

    Mammalian

    Promoter

    U6

    Available Since

    Dec. 5, 2016

    Availability

    Academic Institutions and Nonprofits only

    

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11. pME-Cas9

    Plasmid

    #64237

    Purpose

    expresses codon optimized Cas9 in zebrafish

    Depositor

    Wenbiao Chen

    Article

    Yin et al Genetics. 2015 Apr 8. pii: genetics.115.

    Insert

    codon optimized Cas9
    

    Use

    CRISPR

    Available Since

    May 4, 2015

    Availability

    Academic Institutions and Nonprofits only

    

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12. pChimera

    Plasmid

    #61476

    Purpose

    Vector for conventional cloning that contains AtU6-26 promoter and sgRNA backbone

    Depositor

    Holger Puchta

    Article

    Fauser et al Plant J. 2014 Jul;79(2):348-59. doi: 10.

    Insert

    U6-26:sgRNA
    

    Use

    CRISPR

    Expression

    Plant

    Promoter

    U6-26

    Available Since

    Sept. 24, 2018

    Availability

    Academic Institutions and Nonprofits only

    

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13. pCCM935

    Plasmid

    #58202

    Purpose

    Expresses unc-22 sgRNA in C.elegans. Can be used in Co-CRISPR experiments.

    Depositor

    Craig Mello

    Article

    Kim et al Genetics. 2014 May 30. pii: genetics.114

    Insert

    unc-22 sgRNA
    

    Expression

    Worm

    Promoter

    U6

    Available Since

    July 30, 2014

    Availability

    Academic Institutions and Nonprofits only

14. pX330.LoxP

    Plasmid

    #66586

    Purpose

    sgRNA for LSL

    Depositor

    Wen Xue

    Article

    Li et al Genome Biol. 2015 May 28;16(1):111.

    Insert

    LoxP sgRNA
    

    Expression

    Mammalian

    Available Since

    Aug. 11, 2015

    Availability

    Academic Institutions and Nonprofits only

15. pCCM937

    Plasmid

    #58982

    Purpose

    Expresses avr-15 sgRNA in C.elegans.

    Depositor

    Craig Mello

    Article

    Kim et al Genetics. 2014 May 30. pii: genetics.114

    Insert

    avr-15 sgRNA
    

    Use

    CRISPR

    Expression

    Worm

    Promoter

    U6

    Available Since

    July 30, 2014

    Availability

    Academic Institutions and Nonprofits only

16. pCCM936

    Plasmid

    #58981

    Purpose

    Expresses avr-14 sgRNA in C.elegans.

    Depositor

    Craig Mello

    Article

    Kim et al Genetics. 2014 May 30. pii: genetics.114

    Insert

    avr-14 sgRNA
    

    Use

    CRISPR

    Expression

    Worm

    Promoter

    U6

    Available Since

    July 30, 2014

    Availability

    Academic Institutions and Nonprofits only

17. hU6-Sa_tracr

    Plasmid

    #135973

    Purpose

    Contains tracrRNA-hU6 sequence for double sgRNA cloning

    Depositor

    Yongming Wang

    Type

    Empty backbone

    Expression

    Mammalian

    Available Since

    Nov. 10, 2020

    Availability

    Academic Institutions and Nonprofits only

    

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18. pRPCN

    Plasmid

    #193726

    Purpose

    Expresses Cas9 from RK2 origin with RFP-marked sgRNA insertion site and kanR/sacB selection/counterselection

    Depositor

    Martin Buck

    Type

    Empty backbone

    Use

    CRISPR and Synthetic Biology

    Expression

    Bacterial

    Available Since

    Feb. 16, 2023

    Availability

    Academic Institutions and Nonprofits only

    

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19. pLenti-U6-tdTomato-P2A-BlasR (LRT2B)

    Plasmid

    #110854

    Purpose

    Lentiviral vector for constitutive expression of sgRNAs; includes tdTomato and BlasticidinS resistance markers

    Depositor

    Lukas Dow

    Article

    Zafra et al Nat Biotechnol. 2018 Jul 3. pii: nbt.419

    Insert

    sgRNA scaffold with spacer
    

    Use

    Lentiviral

    Mutation

    WT

    Promoter

    U6

    Available Since

    June 27, 2018

    Availability

    Academic Institutions and Nonprofits only

    

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20. pU6-(BbsI)_CBh-Cas9-T2A-mCherry

    Plasmid

    #64324

    Purpose

    Expression vector for sgRNAs cloned into the BbsI sites and for expression of Cas9 linked to mCherry via a T2A peptide

    Depositor

    Ralf Kuehn

    Article

    Chu et al Nat Biotechnol. 2015 Mar 24. doi: 10.103

    Inserts

    Cas9
    sgRNA cassette
    

    Use

    CRISPR

    Tags

    3xFLAG, NLS, and T2A-mCherry

    Expression

    Mammalian

    Promoter

    CBh and U6

    Available Since

    Sept. 2, 2015

    Availability

    Academic Institutions and Nonprofits only

    

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