We narrowed to 32,170 results for: uros

1. pLV hUbC-dSaCas9-KRAB-T2A-PuroR

   Plasmid

   #106249

   Purpose

   Lentiviral vector with puro selection for expression of S. aureus dCas9-KRAB

   Depositor

   Charles Gersbach

   Article

   Thakore et al Nat Commun. 2018 Apr 26;9(1):1674. doi:

   Insert

   dead S. aureus Cas9 KRAB T2A PuroR
   

   Use

   CRISPR and Lentiviral

   Tags

   HA-NLS and NLS-KRAB

   Expression

   Mammalian

   Mutation

   D10A and N580A

   Promoter

   hUbC

   Available Since

   May 11, 2018

   Availability

   Academic Institutions and Nonprofits only

   

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2. FLAG-LoxP-PURO-LoxP-HaloTERT WT HR Donor

   Plasmid

   #86843

   Purpose

   HR Donor for N-terminal FLAG-HaloTag-TERT fusion in human cells. Includes Puro selection marker flanked by LoxP sites.

   Depositor

   Thomas Cech

   Article

   Schmidt et al Cell. 2016 Aug 25;166(5):1188-1197.e9. d

   Insert

   FLAG-HaloTag-TERT Exon 1 and part of Exon 2 (TERT Human)
   

   Tags

   FLAG-HaloTag

   Expression

   Mammalian

   Promoter

   Endogenous TERT promoter

   Available Since

   March 24, 2017

   Availability

   Academic Institutions and Nonprofits only

   

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3. loxP-Puro-loxP-3xV5-SNAP-Rab11 HR

   Plasmid

   #229677

   Purpose

   Homology repair plasmid for endogenous tagging of Rab11 at the N-terminus with SNAP tag and a 3x V5 epitope tag. Contains a puromycin resistance cassette for selection of edited cells.

   Depositor

   Francesca Bottanelli

   Article

   Stockhammer et al Nat Cell Biol. 2024 Oct 4. doi: 10.1038/

   Insert

   genomic sequence Rab11 locus (RAB11A Human)
   

   Use

   CRISPR

   Tags

   SNAP, 3xV5

   Available Since

   Jan. 3, 2025

   Availability

   Academic Institutions and Nonprofits only

   

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4. pcDNA4TO-K560-E236A-24xGCN4_v1-IRES-Puro

   Plasmid

   #60909

   Purpose

   A minimal K560 kinesin rigor motor tagged with the SunTag for single molecule imaging

   Depositor

   Ron Vale

   Article

   Tanenbaum et al Cell. 2014 Oct 8. pii: S0092-8674(14)012

   Insert

   K560-E236A
   

   Expression

   Mammalian

   Available Since

   Nov. 18, 2014

   Availability

   Academic Institutions and Nonprofits only

   

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5. pLV_hU6-sgRNA_hUbC-dSaCas9-KRAB-T2A-PuroR

   Plasmid

   #162334

   Purpose

   Lentiviral expression of dSaCas9-KRAB and a sgRNA with puromycin resistance

   Depositor

   Charles Gersbach

   Article

   Black et al Cell Rep. 2020 Dec 1;33(9):108460. doi:

   Insert

   dSaCas9-KRAB
   

   Use

   Lentiviral

   Tags

   HA

   Expression

   Mammalian

   Promoter

   hUbC

   Available Since

   Feb. 11, 2021

   Availability

   Academic Institutions and Nonprofits only

   

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6. pLenti-C-Myc-DDK-IRES-Puro HNRNPA1

   Plasmid

   #200727

   Purpose

   Lentiviral expression of Flag-Tagged human HNRNPA1

   Depositor

   Claudio Alarcón

   Insert

   HNRNPA1 (HNRNPA1 Human)
   

   Use

   Lentiviral

   Tags

   Myc-DDK from backbone plasmid

   Expression

   Mammalian

   Promoter

   CMV

   Available Since

   July 25, 2023

   Availability

   Academic Institutions and Nonprofits only

   

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7. pSUPER-retro-puro-tetO-CTR1-shRNA

   Plasmid

   #53180

   Purpose

   Expresses shRNA against human CTR1 from puromycin resistance retroviral vector

   Depositor

   Christopher Counter

   Article

   Brady et al Nature. 2014 Apr 9. doi: 10.1038/nature1

   Insert

   CTR1 (SLC31A1 Human)
   

   Use

   RNAi and Retroviral

   Expression

   Mammalian

   Available Since

   Sept. 19, 2014

   Availability

   Academic Institutions and Nonprofits only

8. pcDNA-puro-TagBFP-β-ACTIN-3′UTR-8xMS2

   Plasmid

   #205160

   Purpose

   express β-ACTIN-3′UTR-8xMS2

   Depositor

   Weirui Ma

   Article

   Hu et al Elife. 2023 Mar 3;12:e82178. doi: 10.755

   Insert

   β-ACTIN-3′UTR-24xMS2
   

   Tags

   tagBFP

   Expression

   Bacterial and Mammalian

   Promoter

   CMV

   Available Since

   Oct. 18, 2023

   Availability

   Academic Institutions and Nonprofits only

   

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9. pLVX-EF1a-EGFP-RAB3C-IRES-Puromycin

   Plasmid

   #134857

   Purpose

   Expresses EGFP-tagged synaptic vesicle marker

   Depositor

   David Andrews

   Article

   Schormann et al J Cell Biol. 2020 Mar 2;219(3). pii: 133

   Insert

   Ras-related protein 3C
   

   Use

   Lentiviral

   Tags

   EGFP

   Expression

   Mammalian

   Promoter

   EF1a

   Available Since

   Jan. 26, 2021

   Availability

   Academic Institutions and Nonprofits only

   

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10. pLVX-EF1alpha-nCoV2019-N-meGFP-IRES-Puro

    Plasmid

    #185451

    Purpose

    Expresses SARS-CoV-2 nucleocapsid protein with a c-terminal monomeric EGFP in mammalian cells

    Depositor

    Ahmet Yildiz

    Article

    Jack et al PLoS Biol. 2021 Oct 11;19(10):e3001425.

    Insert

    Nucleocapsid (N SARS-CoV-2)
    

    Use

    Lentiviral

    Tags

    mEGFP

    Expression

    Mammalian

    Promoter

    EF1alpha

    Available Since

    June 22, 2022

    Availability

    Academic Institutions and Nonprofits only

    

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11. CAG-DDdCas9VP192-T2A-EGFP-ires-puro

    Plasmid

    #69534

    Purpose

    DHFR destabilised domain (DD) fused to dCas9VP192 (S.pyogenes) on CAG expression vector. DDdCas9VP192 protein is stabilised by Trimethoprim.

    Depositor

    Timo Otonkoski

    Article

    Balboa et al Stem Cell Reports. 2015 Sep 8;5(3):448-5

    Insert

    DD-dCas9VP192-T2A-EGFP
    

    Tags

    C-term T2A-EGFP and DHFR Destabilised Domain

    Expression

    Mammalian

    Promoter

    CAG

    Available Since

    Nov. 20, 2015

    Availability

    Academic Institutions and Nonprofits only

    

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12. SOX9-T2A-NLS-tdTomato-PGK-Puro

    Plasmid

    #89993

    Purpose

    Donor template for generation of SOX9-ntdTomato reporter cell lines

    Depositor

    Timo Otonkoski

    Insert

    SOX9-T2A-NLS-TdTomato-PGK-Puro (SOX9 Human)
    

    Use

    CRISPR

    Promoter

    none

    Available Since

    April 2, 2020

    Availability

    Academic Institutions and Nonprofits only

    

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13. delta PE OCT4-GFP-2A-PURO transgenic reporter

    Plasmid

    #52380

    Purpose

    BAC recombineering generated construct, used as a reporter for OCT4 expression

    Depositor

    Jacob Hanna

    Article

    Gafni et al Nature. 2013 Dec 12;504(7479):282-6. doi

    Inserts

    GFP-2A-puro
    OCT4 (POU5F1 Human)
    

    Expression

    Mammalian

    Mutation

    deleted -1620 - -1011 upstream from OCT4 ATG

    Available Since

    June 6, 2014

    Availability

    Academic Institutions and Nonprofits only

    

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14. pLVX-EF1a-EGFP-TA MAO-IRES-Puromycin

    Plasmid

    #133025

    Purpose

    Expresses EGFP-tagged tail anchor sequence of monoamine oxidase A

    Depositor

    David Andrews

    Article

    Schormann et al J Cell Biol. 2020 Mar 2;219(3). pii: 133

    Insert

    Monoamine oxidase type A (MAOA Human)
    

    Use

    Lentiviral

    Tags

    EGFP

    Expression

    Mammalian

    Mutation

    deleted amino acids 1-488

    Promoter

    Human elongation factor 1 alpha

    Available Since

    Feb. 7, 2020

    Availability

    Academic Institutions and Nonprofits only

    

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15. pEpicVector3mC_Puro-T2A-mCherry-StrepTagII-ProtC-AU1

    Plasmid

    #162111

    Purpose

    mCherry linked to 3 tags on the C-terminus as barcodes. Puromycin resistant.

    Depositor

    Julien Sage

    Article

    Rovira-Clave et al Cancer Cell. 2022 Nov 14;40(11):1423-143

    Insert

    Puromycin resistant; mCherry linked to StrepTagII-ProtC-AU1
    

    Use

    Lentiviral

    Tags

    StrepTagII-ProtC-AU1

    Mutation

    WT

    Promoter

    EF1A

    Available Since

    Sept. 30, 2022

    Availability

    Academic Institutions and Nonprofits only

    

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16. pEpicVector3G_Puro-T2A-EGFP-StrepTagII-ProtC-FLAG

    Plasmid

    #162090

    Purpose

    EGFP linked to 3 tags on the C-terminus as barcodes. Puromycin resistant.

    Depositor

    Julien Sage

    Article

    Rovira-Clave et al Cancer Cell. 2022 Nov 14;40(11):1423-143

    Insert

    Puromycin resistant; EGFP linked to StrepTagII-ProtC-FLAG
    

    Use

    Lentiviral

    Tags

    StrepTagII-ProtC-FLAG

    Mutation

    WT

    Promoter

    EF1A

    Available Since

    Sept. 30, 2022

    Availability

    Academic Institutions and Nonprofits only

    

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17. pEpicVector3G_Puro-T2A-EGFP-ProtC-FLAG-AU1

    Plasmid

    #162097

    Purpose

    EGFP linked to 3 tags on the C-terminus as barcodes. Puromycin resistant.

    Depositor

    Julien Sage

    Article

    Rovira-Clave et al Cancer Cell. 2022 Nov 14;40(11):1423-143

    Insert

    Puromycin resistant; EGFP linked to ProtC-FLAG-AU1
    

    Use

    Lentiviral

    Tags

    ProtC-FLAG-AU1

    Mutation

    WT

    Promoter

    EF1A

    Available Since

    Sept. 30, 2022

    Availability

    Academic Institutions and Nonprofits only

    

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18. pEpicVector3G_Puro-T2A-EGFP-VSVg-StrepTagII-AU1

    Plasmid

    #162082

    Purpose

    EGFP linked to 3 tags on the C-terminus as barcodes. Puromycin resistant.

    Depositor

    Julien Sage

    Article

    Rovira-Clave et al Cancer Cell. 2022 Nov 14;40(11):1423-143

    Insert

    Puromycin resistant; EGFP linked to VSVg-StrepTagII-AU1
    

    Use

    Lentiviral

    Tags

    VSVg-StrepTagII-AU1

    Mutation

    WT

    Promoter

    EF1A

    Available Since

    Sept. 30, 2022

    Availability

    Academic Institutions and Nonprofits only

    

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19. untargeted Aurora B FRET sensor Kif2 substrate

    Plasmid

    #45215

    Depositor

    Michael Lampson

    Article

    Fuller et al Nature. 2008 Jun 19;453(7198):1132-6. do

    Insert

    untargeted Aurora B FRET sensor
    

    Tags

    CyPET, YPET

    Expression

    Mammalian

    Promoter

    t7

    Available Since

    May 30, 2013

    Availability

    Academic Institutions and Nonprofits only

    

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20. pLV-EF1a-mTagBFP2-2A-Puro_hU6-RfxDR36-BsmBI

    Plasmid

    #226011

    Purpose

    CasRx guide RNA cloning backbone (lenti)

    Depositor

    Silvana Konermann

    Article

    Wei et al Cell Syst. 2023 Dec 20;14(12):1087-1102.

    Type

    Empty backbone

    Use

    Lentiviral

    Available Since

    Oct. 18, 2024

    Availability

    Academic Institutions and Nonprofits only

    

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