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We narrowed to 14,561 results for: cas9 genes

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Showing: 3121 - 3140 of 14561 results

  1. N-Terminal Split Cas9 D10A Nickase with GyrA intein

    Plasmid
    #58695
    Purpose
    Expresses N-terminus of D10A SpCas9 nickase domain fused to a GyrA intein, flanked by ITRs for AAV packaging. Combine with C-Terminal Split Cas9 Gyra Intein for full length SpCas9 nickase production
    Depositor
    Gang Bao
    Article
    Fine et al Sci Rep. 2015 Jul 1;5:10777. doi: 10.103
    Insert
    D10A Nickase humanized S. pyogenes Cas9 with Gyra Nsplit Intein
    Use
    AAV and CRISPR
    Tags
    GyrA Nsplit Intein, HA Tag, and NLS
    Expression
    Mammalian
    Mutation
    D10A nickase converting mutation to SpCas9
    Promoter
    CBh
    Available Since
    Sept. 25, 2014
    Availability
    Academic Institutions and Nonprofits only
    58695-plasmid-map-sequence-id-90694

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  5. T1-araC-Pbad-D10A/H840A cas9 ABCD-pACYC-BB

    Plasmid
    #110547
    Purpose
    CRISPR interference; works in conjunction with an sgRNA expression vector to silence genes in the E. coli chromosome.
    Depositor
    Ichiro Matsumura
    Insert
    dCas9
    Expression
    Bacterial
    Mutation
    D10A, H840A
    Promoter
    pBAD
    Available Since
    July 24, 2018
    Availability
    Academic Institutions and Nonprofits only
    110547-plasmid-map-sequence-id-210556

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  6. pSLPB2-SpdCas9-tagRFPt-P2A-tagBFP-PGK-Blasticidin

    Plasmid
    #187945
    Purpose
    dCas9 fused with tagRFPt, P2A site and tagBFP, expressed under EF1-alpha promoter in a piggyBac plasmid. Expresses Blasticidin resistance under PGK promoter.
    Depositor
    Edda Schulz
    Article
    Noviello et al Nat Commun. 2023 Jun 3;14(1):3225. doi:
    Insert
    SpdCas9-tagRFPt-P2A-tagBFP
    Use
    Synthetic Biology; Piggybac
    Tags
    NLS, NLS + HA-tag, P2A, tagBFP, and tagRFPt
    Expression
    Mammalian
    Available Since
    Oct. 14, 2022
    Availability
    Academic Institutions and Nonprofits only
    187945-plasmid-map-sequence-id-373705

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  7. pLV-hU6-ZNF865-hUbC-dCas9-KRAB-eGFP

    Plasmid
    #241308
    Purpose
    3rd gen lenti vector encoding dCas9 (s. Pyogenes) fused with the KRAB and a 2A eGFP fluorescence marker. Includes guide for suppression of the ZNF865 gene
    Depositor
    Robby Bowles
    Insert
    ZNF865 sgRNA (ZNF865 Human)
    Use
    Lentiviral
    Available Since
    Dec. 8, 2025
    Availability
    Academic Institutions and Nonprofits only
    241308-plasmid-map-sequence-id-483070

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  8. pLenti KO Luc Firefly 2xgRNA - spCas9 iRFP670 puro

    Plasmid
    #166134
    Purpose
    This plasmid allows efficient KO of the firefly luciferase gene, while expressing the far red fluorescent protein iRFP670 and puromycin resistance
    Depositor
    Raphael Gaudin
    Article
    Deffieu et al Hepatology. 2022 Apr 7. doi: 10.1002/hep
    Insert
    hU6-gRNA and hH1-gRNA targeting firefly luciferase gene
    Use
    CRISPR and Lentiviral
    Tags
    iRFP670
    Expression
    Mammalian
    Available Since
    April 11, 2022
    Availability
    Academic Institutions and Nonprofits only
    166134-plasmid-map-sequence-id-333283

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  14. pX458-Dual-Guide-Donor-Cas9-H2B-mCherry

    Plasmid
    #175570
    Purpose
    Donor shuttle plasmid to enable ligation of a second U6-GuideRNA casssette into any pX458 family backbone via digestion of both plasmids with XbaI and KpnI.
    Depositor
    Joseph Brzezinski
    Type
    Empty backbone
    Use
    CRISPR
    Mutation
    XbaI site added upstream of U6 promotor. G to C …
    Available Since
    Oct. 7, 2021
    Availability
    Academic Institutions and Nonprofits only
    175570-plasmid-map-sequence-id-344992

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Showing: 3121 - 3140 of 14561 results