167,601 results

1. pQE-60 roGFP2-Orp1-His

   Plasmid

   #64976

   Purpose

   Bacterial expression of His-tagged roGFP2-Orp1

   Depositor

   Tobias Dick

   Article

   Gutscher et al J Biol Chem. 2009 Nov 13;284(46):31532-4

   Insert

   Orp1 (HYR1 Budding Yeast)
   

   Tags

   His and roGFP2

   Expression

   Bacterial

   Available Since

   June 16, 2015

   Availability

   Academic Institutions and Nonprofits only

   

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2. ND4.2-Left TALE-G1397-N-DddA11-mCherry

   Plasmid

   #179682

   Purpose

   Expression of base editor in mammalian cells and for cell sorting

   Depositor

   David Liu

   Article

   Mok et al Nat Biotechnol. 2022 Sep;40(9):1378-1387

   Insert

   COX8a MTS_3xFLAG_ND4.2 Left TALE_2aa_DddA G1397-N (S1330I A1341V N1342S E1370K T1380I)_1xUGI_P2A_mCherry_ATP5B 3'UTR
   

   Expression

   Mammalian

   Mutation

   S1330I A1341V N1342S E1370K T1380I

   Available Since

   April 4, 2022

   Availability

   Academic Institutions and Nonprofits only

   

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3. pAAV-CAG-SomaGCaMP6f2 (AAV9)

   Viral Prep

   #158757-AAV9

   Purpose

   Ready-to-use AAV9 particles produced from pAAV-CAG-SomaGCaMP6f2 (#158757). In addition to the viral particles, you will also receive purified pAAV-CAG-SomaGCaMP6f2 plasmid DNA. CAG-driven expression of soma-targeting GCaMP6f2. These AAV preparations are suitable purity for injection into animals.

   Depositor

   Edward Boyden

   Article

   Shemesh et al Neuron. 2020 Jun 26. pii: S0896-6273(20)

   Promoter

   CAG promoter

   Available Since

   Jan. 6, 2022

   Availability

   Academic Institutions and Nonprofits only

4. VPX-mRNA

   Plasmid

   #227679

   Purpose

   plasmid for VPX mRNA IVT

   Depositor

   Scot Wolfe

   Article

   Liu et al Nat Biotechnol. 2024 Sep 25. doi: 10.103

   Insert

   VPX (HIV2gp3 )
   

   Expression

   Mammalian

   Available Since

   Oct. 24, 2024

   Availability

   Academic Institutions and Nonprofits only

   

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5. STICR_IndexE

   Pooled Library

   #180483

   Purpose

   High Complexity Lentiviral Barcode Library

   Depositor

   Tomasz Nowakowski

   Article

   Delgado et al Nature. 2021 Dec 15. pii: 10.1038/s41586

   Expression

   Mammalian

   Species

   Synthetic

   Use

   Lentiviral

   Available Since

   Sept. 12, 2022

   Availability

   Academic Institutions and Nonprofits only

6. pLenti_PEmax-2A-MLH1dn-2A-BSD

   Plasmid

   #234072

   Purpose

   For lentiviral expression of PEmax with MLH1dn and blasticidin resistance

   Depositor

   Greg Findlay

   Article

   Herger et al Cell Genom. 2025 Mar 18:100814. doi: 10.

   Insert

   PEmax-2A-MLH1dn
   

   Use

   CRISPR and Lentiviral

   Tags

   SV40 bpNLS and c-Myc NLS

   Expression

   Mammalian

   Promoter

   EF-1α core promoter

   Available Since

   March 26, 2025

   Availability

   Academic Institutions and Nonprofits only

   

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7. E. coli BL21 (DE3) ΔEntD

   Bacterial Strain

   #192874

   Purpose

   Deletion of native E. coli EntD, a 4'-PPTase required for native enterobactin synthesis. Allows expression of NRPS carrier proteins that do not harbor a phosphopantetheinyl group.

   Depositor

   Christian Chalut, Christophe Guilhot

   Article

   Chalut et al Proc Natl Acad Sci U S A. 2006 May 30;10

   Bacterial Resistance

   None

   Species

   Escherichia coli

   Available Since

   Sept. 7, 2023

   Availability

   Academic Institutions and Nonprofits only

8. pT3-EF1a-c-Met

   Plasmid

   #31784

   Depositor

   Xin Chen

   Article

   Tward et al Proc Natl Acad Sci U S A. 2007 Sep 11;10

   Insert

   Met (MET Human)
   

   Expression

   Mammalian

   Promoter

   EF1alpha

   Available Since

   Oct. 21, 2011

   Availability

   Academic Institutions and Nonprofits only

   

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9. tdTomato-N1

   Plasmid

   #54642

   Purpose

   Localization: N1 Cloning Vector, Excitation: 554, Emission: 581

   Depositor

   Roger Tsien, Nathan Shaner, Michael Davidson

   Article

   Shaner et al Nat Biotechnol. 2004 Dec;22(12):1567-72.

   Has Service

   Cloning Grade DNA

   Type

   Empty backbone

   Tags

   tdTomato

   Expression

   Mammalian

   Promoter

   CMV

   Available Since

   June 20, 2014

   Availability

   Academic Institutions and Nonprofits only

   

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10. pRL443

    Plasmid

    #70261

    Purpose

    Shuttle vector suitable for mobilizing plasmids to Anabaena (Nostoc) sp. strain PCC 7120 and other cyanobacterial strains.

    Depositor

    Peter Wolk

    Article

    Elhai et al J Bacteriol. 1997 Mar;179(6):1998-2005.

    Type

    Empty backbone

    Available Since

    Feb. 1, 2016

    Availability

    Academic Institutions and Nonprofits only

    

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11. cytoplasmic cpVenus control

    Plasmid

    #186788

    Purpose

    Identical to cytoplasmic NAD+ biosensor, sans bipartite NAD+-binding domain modeled from bacterial DNA ligase

    Depositor

    Michael Cohen

    Article

    Cambronne et al Science. 2016 Jun 17;352(6292):1474-7. d

    Insert

    cytoplasmic cpVenus control
    

    Use

    Lentiviral

    Expression

    Mammalian

    Available Since

    July 28, 2022

    Availability

    Academic Institutions and Nonprofits only

    

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12. pEGFP-mKOR

    Plasmid

    #179285

    Purpose

    Express mouse kappa opioid receptor (GFP tagged) in mammalian cells

    Depositor

    Christian Gruber

    Article

    Muratspahic et al J Med Chem. 2021 Jul 8;64(13):9042-9055.

    Insert

    kappa opioid receptor (Oprk1 Mouse)
    

    Tags

    EGFP

    Expression

    Mammalian

    Promoter

    CMV

    Available Since

    Feb. 7, 2022

    Availability

    Academic Institutions and Nonprofits only

    

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13. pJMP1356

    Plasmid

    #119276

    Purpose

    for cloning new sgRNAs

    Depositor

    Jason Peters, Carol Gross, Oren Rosenberg

    Article

    Peters et al Nat Microbiol. 2019 Jan 7. pii: 10.1038/

    Insert

    dcas9
    

    Expression

    Bacterial

    Mutation

    D10A and H840A

    Available Since

    March 5, 2019

    Availability

    Academic Institutions and Nonprofits only

    

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14. pMK344 (Hygro-P2A-mAID)

    Plasmid

    #121178

    Purpose

    N-terminal tagging

    Depositor

    Masato Kanemaki

    Article

    Yesbolatova et al Methods. 2019 Apr 23. pii: S1046-2023(18

    Insert

    Hygro-P2A-mAID
    

    Use

    CRISPR

    Available Since

    Feb. 13, 2019

    Availability

    Academic Institutions and Nonprofits only

    

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15. hLRP1-pEZT-BM

    Plasmid

    #177985

    Purpose

    Expression the full-length ectodomain of human LRP1 [residue 1-4414] in mammalian cells

    Depositor

    Lora Hooper

    Article

    Bang et al Science. 2021 Sep 17;373(6561):eabf9232.

    Insert

    LDL receptor related protein 1 (LRP1 Human)
    

    Tags

    No

    Expression

    Mammalian

    Promoter

    CMV

    Available Since

    Dec. 15, 2021

    Availability

    Academic Institutions and Nonprofits only

    

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16. pBK2044-AAV-2xSp1-2xNFkB-EFSNC-dSaCas9-KRAB-MECP2

    Plasmid

    #223164

    Purpose

    2nd gen. vector for expression of KRAB and truncated MECP2 with dSaCas9 and empty gRNA scaffold

    Depositor

    Boris Kantor

    Article

    Kantor et al bioRxiv [Preprint]. 2024 May 19:2023.04.

    Insert

    KRAB-MECP2 (MECP2 Human)
    

    Use

    AAV and CRISPR

    Tags

    3xFLAG

    Expression

    Mammalian

    Mutation

    Encodes aa 204-310 (MECP2)

    Promoter

    EF1a

    Available Since

    Aug. 21, 2024

    Availability

    Academic Institutions and Nonprofits only

    

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17. pDEST-CMV-3xFLAG-gateway-EGFP

    Plasmid

    #122845

    Purpose

    N-terminal 3xFLAG tag and C-terminal EGFP tag. Gateway destination vector for mammalian expression.

    Depositor

    Robin Ketteler

    Article

    Agrotis et al Autophagy. 2019 Jan 21. doi: 10.1080/155

    Type

    Empty backbone

    Use

    Gateway destination

    Tags

    3xFLAG and EGFP

    Expression

    Mammalian

    Promoter

    CMV

    Available Since

    May 13, 2019

    Availability

    Academic Institutions and Nonprofits only

    

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18. pSNAPf-N1

    Plasmid

    #58187

    Depositor

    Michael Davidson

    Type

    Empty backbone

    Tags

    SNAP tag

    Expression

    Mammalian

    Available Since

    Jan. 13, 2015

    Availability

    Industry, Academic Institutions, and Nonprofits

    

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19. pET30-2-GPD1

    Plasmid

    #83911

    Purpose

    stable overexpression

    Depositor

    David Sabatini

    Article

    Pacold et al Nat Chem Biol. 2016 Jun;12(6):452-8. doi

    Insert

    glycerol-3-phosphate dehydrogenase 1 (GPD1 Human)
    

    Tags

    6x His tags

    Expression

    Bacterial

    Promoter

    T7

    Available Since

    Oct. 21, 2016

    Availability

    Academic Institutions and Nonprofits only

    

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20. pLEX307-APN-blast

    Plasmid

    #158455

    Purpose

    Constitutive lentiviral expression of human APN.

    Depositor

    Sho Iketani, Alejandro Chavez

    Insert

    APN (ANPEP Human)
    

    Use

    Lentiviral

    Expression

    Mammalian

    Available Since

    Aug. 10, 2020

    Availability

    Academic Institutions and Nonprofits only

    

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21. MBP-FUS_FL_WT

    Plasmid

    #98651

    Purpose

    Expresses MBP-tagged full length FUS

    Depositor

    Nicolas Fawzi

    Article

    Burke et al Mol Cell. 2015 Oct 15;60(2):231-41. doi:

    Insert

    FUS (FUS Human)
    

    Tags

    6xHis-MBP

    Expression

    Bacterial

    Available Since

    July 27, 2017

    Availability

    Academic Institutions and Nonprofits only

    

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22. pSBbi 41BBL-CD86 mIL-15

    Plasmid

    #183245

    Purpose

    Expression of human 41BBL, CD86, and membrane bound human IL-15 (IL-15-IL-15Ra) in a Sleeping Beauty transposon vector for use in artificial antigen presenting cells

    Depositor

    Alexander McLellan

    Article

    Dobson et al Immunol Cell Biol. 2023 Aug 16. doi: 10.

    Insert

    41BBL, CD86, mbIL-15 (IL-15-IL-15Ra)
    

    Expression

    Mammalian

    Available Since

    Aug. 18, 2022

    Availability

    Academic Institutions and Nonprofits only

    

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23. pNO286-3

    Plasmid

    #107746

    Purpose

    Key genes: "mini-CcaS #10", ho1, pcyA, spectinomycin resistance, and medium copy backbone (p15a)

    Depositor

    Jeffrey Tabor

    Article

    Ong et al Chembiochem. 2018 Feb 8. doi: 10.1002/cb

    Inserts

    CcaS
    Ho1
    PcyA
    

    Use

    Synthetic Biology

    Expression

    Bacterial

    Mutation

    Deleted AA 223 and 512

    Promoter

    J23106 and J23108

    Available Since

    April 26, 2018

    Availability

    Academic Institutions and Nonprofits only

    

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24. pT7-EGFP-C1-HsPatL1_I

    Plasmid

    #146547

    Purpose

    Mammalian Expression of HsPatL1

    Depositor

    Elisa Izaurralde

    Article

    Braun et al EMBO J. 2010 Jul 21. 29(14):2368-80.

    Insert

    HsPatL1 (PATL1 Human)
    

    Expression

    Mammalian

    Available Since

    Aug. 9, 2021

    Availability

    Academic Institutions and Nonprofits only

    

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25. pCVD442

    Plasmid

    #11074

    Depositor

    James Kaper, Michael Donnenberg

    Article

    Donnenberg et al Infect Immun. 1991 Dec . 59(12):4310-7.

    Insert

    sacB (sacB Bacillus subtilis)
    

    Expression

    Bacterial

    Available Since

    Jan. 19, 2006

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

26. pCFJ104 - Pmyo-3::mCherry::unc-54

    Plasmid

    #19328

    Depositor

    Erik Jorgensen

    Article

    Christian et al

    Insert

    Pmyo-3::mCherry
    

    Tags

    mCherry

    Expression

    Worm

    Available Since

    Nov. 21, 2008

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

27. 6xHis-SNAP-PI(4,5)P2 Probe

    Plasmid

    #211513

    Purpose

    For expression of recombinant biosensor for detection of Phosphatidylinositol-4,5-Bisphosphate (PI(4,5)P2) from bacteria

    Depositor

    Hannes Maib, David Murray

    Article

    Maib et al J Cell Biol. 2024 Jun 3;223(6):e20231009

    Insert

    PLCdelta1-PH
    

    Tags

    6xhis-SNAP

    Expression

    Bacterial

    Available Since

    Jan. 19, 2024

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

28. beta-catenin (S33A, S37A, T41A, S45A)-pcw107-V5

    Plasmid

    #64613

    Purpose

    when used to produce lentivirus, express physiological levels of insert

    Depositor

    Kris Wood, David Sabatini

    Article

    Martz et al Sci Signal. 2014 Dec 23;7(357):ra121. do

    Insert

    Ctnnb1 (Ctnnb1 Mouse)
    

    Use

    Lentiviral

    Tags

    V5

    Mutation

    S33A, S37A, T41A, S45A

    Promoter

    PGK

    Available Since

    May 12, 2015

    Availability

    Academic Institutions and Nonprofits only

    

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29. CMV-LgBit

    Plasmid

    #234738

    Purpose

    Mammalian expression of large NanoBit fragment (LgBit)

    Depositor

    Nevin Lambert

    Article

    Jang et al Elife. 2024 Nov 8;13:RP97033. doi: 10.75

    Insert

    LgBit
    

    Expression

    Mammalian

    Promoter

    CMV

    Available Since

    March 20, 2025

    Availability

    Academic Institutions and Nonprofits only

    

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30. pMX229

    Plasmid

    #23002

    Depositor

    Linda Wordeman

    Article

    Stumpff et al Dev Cell. 2008 Feb . 14(2):252-62.

    Insert

    Kif18A (KIF18A Human)
    

    Tags

    6His and EGFP

    Expression

    Mammalian

    Available Since

    Feb. 8, 2010

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

31. GFP-PH-FAPP1

    Plasmid

    #161986

    Purpose

    Expresses GFP-tagged PH-FAPP1. PI(4)P biosensor

    Depositor

    Tamas Balla, David Bryant

    Article

    Balla et al Mol Biol Cell. 2005 Mar;16(3):1282-95. d

    Insert

    PH-FAPP1 (PLEKHA3 Human)
    

    Tags

    GFP

    Expression

    Mammalian

    Available Since

    Dec. 3, 2020

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

32. pET23a-mUbc9

    Plasmid

    #53137

    Purpose

    E.coli expression of full length untagged mouse Ubc9 (protein sequence identical to human)

    Depositor

    Frauke Melchior

    Article

    Pichler et al Cell. 2002 Jan 11;108(1):109-20.

    Insert

    Ubc9 (Ube2i Mouse)
    

    Tags

    untagged

    Expression

    Bacterial

    Mutation

    protein sequence is identical to human

    Promoter

    T7

    Available Since

    Sept. 17, 2014

    Availability

    Academic Institutions and Nonprofits only

    

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33. pcDNA-Wnt10A

    Plasmid

    #35920

    Depositor

    Marian Waterman

    Article

    Najdi et al Differentiation. 2012 Sep;84(2):203-13.

    Insert

    Wnt10A (WNT10A Human)
    

    Expression

    Mammalian

    Mutation

    None

    Promoter

    CMV

    Available Since

    May 7, 2013

    Availability

    Academic Institutions and Nonprofits only

    

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34. pBS-L1PA1-CH-mneo

    Plasmid

    #51288

    Purpose

    contains the fully codon optimized ORF1 and ORF2 of the human L1RP

    Depositor

    Astrid Roy-Engel

    Article

    Wagstaff et al PLoS One. 2011 May 10;6(5):e19672. doi:

    Insert

    L1 (L1RE1 Human)
    

    Tags

    mneoI indicator cassette containing an inverted n…

    Expression

    Mammalian

    Promoter

    CMV

    Available Since

    March 21, 2014

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

35. pBV-Luc

    Plasmid

    #16539

    Purpose

    luciferase reporter plasmid with very low basal activity

    Depositor

    Bert Vogelstein

    Article

    He et al Cell. 1999 Oct 29. 99(3):335-45.

    Has Service

    Cloning Grade DNA

    Type

    Empty backbone

    Use

    Luciferase

    Tags

    Luciferase

    Expression

    Mammalian

    Available Since

    July 3, 2008

    Availability

    Academic Institutions and Nonprofits only

    

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36. pCMV-dCas13-inactive M3nls

    Plasmid

    #157854

    Purpose

    Targeted m6A RNA methylation in mammalian cells, methyltransferase-inactive mutant

    Depositor

    David Liu

    Article

    Wilson et al Nat Biotechnol. 2020 Jun 29. pii: 10.103

    Insert

    dCas13-inactive dCas13-inactive M3nls (METTL3 Cas13b is from Prevotella sp. P5-125; METTL3 is from H. sapiens)
    

    Expression

    Mammalian

    Mutation

    PspCas13b Δ984-1090 H133A; METTL3 D395A

    Available Since

    Aug. 4, 2020

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

37. pET_RTX_(exo-)

    Plasmid

    #102786

    Purpose

    Expresses the Reverse Transcriptase gene (3'-5' exonuclease deficient mutant)

    Depositor

    Andrew Ellington

    Article

    Ellefson et al Science. 2016 Jun 24;352(6293):1590-3. d

    Insert

    reverse transcription xenopolymerase with 3'-5' deficient exonuclease
    

    Expression

    Bacterial

    Available Since

    July 3, 2018

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

38. pBT375

    Plasmid

    #125616

    Purpose

    Expresses evoAPOBEC1-BE4max-NG in mammalian cells

    Depositor

    David Liu

    Article

    Thuronyi et al Nat Biotechnol. 2019 Jul 22. pii: 10.103

    Insert

    evoAPOBEC1-BE4max-NG
    

    Expression

    Mammalian

    Mutation

    E4K H109N H122L D124N R154H A165S P201S F205S

    Available Since

    July 25, 2019

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

39. pAAV-DIO-CAG-hM4Di-P2A-dTomato

    Plasmid

    #202037

    Purpose

    AAV-mediated, cre-dependent expression of the pharmacogenetic actuator hM4Di-2A-dTomato

    Depositor

    Peter Jonas, Yoav Ben-Simon

    Insert

    hM4Di
    

    Use

    AAV

    Tags

    dTomato

    Promoter

    CAG

    Available Since

    Aug. 31, 2023

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

40. pLV hUbC-dCas9-MQ1(Q147L)-EGFP

    Plasmid

    #89793

    Purpose

    Targeted CpG methylation

    Depositor

    Margaret Goodell

    Article

    Lei et al Nat Commun. 2017 Jul 11;8:16026. doi: 10

    Insert

    site-specific DNA-methyltransferase SssI
    

    Use

    CRISPR and Lentiviral

    Tags

    3*FLAG and T2A-EGFP

    Expression

    Mammalian

    Mutation

    Q147L, CAA-->CTA

    Promoter

    hUbC

    Available Since

    July 12, 2017

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge