We narrowed to 14,208 results for: CHL

1. pBSFI-myr-akt-T308D/S473D

   Plasmid

   #49192

   Purpose

   cDNA of Akt1 with myristyolation signal and point mutation from T to D at position 308 and S to D at position 473 in cloning vector

   Depositor

   Peter Vogt

   Article

   Aoki et al Proc Natl Acad Sci U S A. 1998 Dec 8;95(

   Insert

   Akt1 (Akt1 Mouse)
   

   Use

   Adaptor plasmid

   Tags

   HA tag and myristolation signal

   Expression

   Mutation

   T308D; S473D

   Promoter

   T7

   Available since

   Dec. 13, 2013

   Availability

   Academic Institutions and Nonprofits only

   

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2. HA-EglN3-pcDNA3

   Plasmid

   #18960

   Depositor

   William Kaelin

   Article

   Lee et al Cancer Cell. 2005 Aug . 8(2):155-67.

   Insert

   Egg laying Nine - 3 (EGLN3 Human)
   

   Use

   Tags

   HA-tag

   Expression

   Mammalian

   Mutation

   wt cDNA

   Promoter

   Available since

   Aug. 20, 2008

   Availability

   Academic Institutions and Nonprofits only

   

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3. pET30a-His6-LOXCAT

   Plasmid

   #140084

   Purpose

   Fusion of A. viridans lactate oxidase (LOX) and E. coli catalase (CAT) that converts lactate into pyruvate without producing H2O2.

   Depositor

   Vamsi Mootha

   Article

   Patgiri et al Nat Biotechnol. 2020 Jan 13. pii: 10.103

   Insert

   A fusion of lactate oxidase from A. viridans and catalase from E. coli
   

   Use

   Tags

   His6

   Expression

   Bacterial

   Mutation

   Promoter

   T7

   Available since

   Sept. 16, 2020

   Availability

   Academic Institutions and Nonprofits only

   

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4. HA-EglN2-pcDNA3

   Plasmid

   #18961

   Depositor

   William Kaelin

   Article

   Lee et al Cancer Cell. 2005 Aug . 8(2):155-67.

   Insert

   Egg laying Nine - 2 (EGLN2 Human)
   

   Use

   Tags

   HA-tag

   Expression

   Mammalian

   Mutation

   wt cDNA

   Promoter

   Available since

   June 19, 2009

   Availability

   Academic Institutions and Nonprofits only

   

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5. pLKO-CreERT2 sgRipk4 v3

   Plasmid

   #158048

   Purpose

   lenti-viral construct with tamoxifen inducible Cre recombinase and U6 driven sgRNA against mouse Ripk4. NO Cas9

   Depositor

   Daniel Schramek

   Article

   Loganathan et al Science. 2020 Mar 13;367(6483):1264-1269

   Insert

   Ripk4 sgRNA
   

   Use

   Tags

   Expression

   Mammalian

   Mutation

   Promoter

   U6

   Available since

   Oct. 5, 2020

   Availability

   Industry, Academic Institutions, and Nonprofits

   

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6. pLKO-CreERT2 sgAdam10 v3

   Plasmid

   #158047

   Purpose

   lenti-viral construct with tamoxifen inducible Cre recombinase and U6 driven sgRNA against mouse Adam10. NO Cas9

   Depositor

   Daniel Schramek

   Article

   Loganathan et al Science. 2020 Mar 13;367(6483):1264-1269

   Insert

   Adam10 sgRNA
   

   Use

   Tags

   Expression

   Mammalian

   Mutation

   Promoter

   U6

   Available since

   Oct. 5, 2020

   Availability

   Industry, Academic Institutions, and Nonprofits

   

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7. pAAV-Ef1a-DIO hChR2(E123T/T159C)-mCherry

   Plasmid

   #35510

   Purpose

   AAV expression of Ef1a-driven, cre-dependent, hChR2 variant for ultrafast optogenetic control.

   Depositor

   Karl Deisseroth

   Article

   Mattis et al Nat Methods. 2011 Dec 18;9(2):159-72. do

   Insert

   hChR2
   

   Use

   AAV

   Tags

   mCherry

   Expression

   Mammalian

   Mutation

   E123T and T159C

   Promoter

   Ef1a

   Available since

   April 18, 2012

   Availability

   Academic Institutions and Nonprofits only

   

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8. tetO-MEF2C

   Plasmid

   #46031

   Depositor

   John Gearhart

   Article

   Addis et al J Mol Cell Cardiol. 2013 Apr 13;60C:97-1

   Insert

   MEF2C (MEF2C Human)
   

   Use

   Lentiviral

   Tags

   His and V5

   Expression

   Mammalian

   Mutation

   Promoter

   tetO

   Available since

   July 9, 2013

   Availability

   Academic Institutions and Nonprofits only

   

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9. pCAG-ChrimsonR-tdT

   Plasmid

   #59169

   Purpose

   Expression vector for ChrimsonR-tdTomato

   Depositor

   Edward Boyden

   Article

   Klapoetke et al Nat Methods. 2014 Mar;11(3):338-46. doi:

   Insert

   ChrimsonR-tdTomato
   

   Use

   Tags

   tdTomato

   Expression

   Mammalian

   Mutation

   K176R

   Promoter

   CAG

   Available since

   Aug. 29, 2014

   Availability

   Academic Institutions and Nonprofits only

   

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10. pIRES2-hPAC-FLAG

    Plasmid

    #129494

    Purpose

    Expresses human TMEM206 tagged at the C-terminus with FLAG

    Depositor

    Zhaozhu Qiu

    Article

    Yang et al Science. 2019 Apr 26;364(6438):395-399.

    Insert

    human PAC tagged with FLAG tag at the C-terminal end (PACC1 Human)
    

    Use

    Tags

    FLAG

    Expression

    Mammalian

    Mutation

    Promoter

    CMV

    Available since

    Sept. 13, 2019

    Availability

    Academic Institutions and Nonprofits only

    

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11. tetO-GATA4

    Plasmid

    #46030

    Depositor

    John Gearhart

    Article

    Addis et al J Mol Cell Cardiol. 2013 Apr 13;60C:97-1

    Insert

    GATA4 (GATA4 Human)
    

    Use

    Lentiviral

    Tags

    His and V5

    Expression

    Mammalian

    Mutation

    R43Q, G50S and N197S

    Promoter

    tetO

    Available since

    July 24, 2013

    Availability

    Academic Institutions and Nonprofits only

    

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12. pWPXL-c-Myc

    Plasmid

    #36980

    Depositor

    Bob Weinberg

    Article

    Guo et al Cell. 2012 Mar 2;148(5):1015-28.

    Insert

    c-Myc (Myc Mouse)
    

    Use

    Lentiviral

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    EF1a

    Available since

    July 30, 2012

    Availability

    Academic Institutions and Nonprofits only

13. pSicoR-Ef1a-mCh

    Plasmid

    #31847

    Purpose

    Cre-regulated lentiviral shRNA vector. Cre addition causes both mCherry and shRNA to be recombined out of the construct, turning OFF shRNA expression.

    Depositor

    Bruce Conklin

    Article

    Salomonis et al Proc Natl Acad Sci U S A. 2010 Jun 8;107

    Type

    Empty backbone

    Use

    Cre/Lox, Lentiviral, and RNAi

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    U6 for shRNA; Ef1alpha for mCherry

    Available since

    Jan. 3, 2012

    Availability

    Academic Institutions and Nonprofits only

    

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14. pNatA (pACYCduet-naa10-naa15)

    Plasmid

    #72928

    Purpose

    Allows expression of a modified version of the fission yeast NatA complex - chloramphenicol marker

    Depositor

    Dan Mulvihill

    Article

    Eastwood et al FEBS Lett. 2017 Feb 18. doi: 10.1002/187

    Inserts

    naa10 (naa10 Fission Yeast, Synthetic)
    naa15 (naa15 Fission Yeast)
    

    Use

    Tags

    Expression

    Bacterial

    Mutation

    Codon optomised for E.coli expression

    Promoter

    T7

    Available since

    Feb. 8, 2016

    Availability

    Academic Institutions and Nonprofits only

    

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15. pET30a-His6-LOXCATmut

    Plasmid

    #140085

    Purpose

    A control for His6-LOXCAT where LOX is catalytically dead (mutations H265A and R268A)

    Depositor

    Vamsi Mootha

    Article

    Patgiri et al Nat Biotechnol. 2020 Jan 13. pii: 10.103

    Insert

    A fusion of lactate oxidase from A. viridans and catalase from E. coli
    

    Use

    Tags

    His6

    Expression

    Bacterial

    Mutation

    Mutation in H265A and R268A in LOX (used original…

    Promoter

    T7

    Available since

    Sept. 16, 2020

    Availability

    Academic Institutions and Nonprofits only

    

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16. pB PUb-integrase

    Plasmid

    #183966

    Purpose

    piggyBac plasmid for insect transformation, expressing PhiC31 integrase under control of the Aedes aegypti PUb promoter, GFP fluorescent marker

    Depositor

    Eric Marois

    Article

    Lutrat et al Commun Biol. 2023 Jun 16;6(1):646. doi:

    Insert

    PhiC31 integrase
    

    Use

    Piggybac insect transgenesis destination vector

    Tags

    Expression

    Mutation

    Promoter

    Aedes aegypti Polyubiquitin

    Available since

    Aug. 9, 2022

    Availability

    Academic Institutions and Nonprofits only

    

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17. pMpGWB337

    Plasmid

    #68061

    Purpose

    Gateway-based vector for Marchantia polymorpha to express a floxed gene, with an expression cassette for Cre-glucocorticoid receptor fusion protein under the control of heat-inducible promoter

    Depositor

    Takayuki Kohchi

    Article

    Nishihama et al Plant Cell Physiol. 2015 Jul 6. pii: pcv

    Type

    Empty backbone

    Use

    Cre/Lox

    Tags

    Expression

    Plant

    Mutation

    Promoter

    MpEF1alpha promoter

    Available since

    Sept. 18, 2015

    Availability

    Academic Institutions and Nonprofits only

    

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18. pAAV-CaMKIIa-hChR2(E123T/T159C)-mCherry

    Plasmid

    #35512

    Purpose

    AAV expression of CaMKIIa-driven hChR2 variant for ultrafast optogenetic control.

    Depositor

    Karl Deisseroth

    Article

    Mattis et al Nat Methods. 2011 Dec 18;9(2):159-72. do

    Insert

    hChR2
    

    Use

    AAV

    Tags

    mCherry

    Expression

    Mammalian

    Mutation

    E123T and T159C

    Promoter

    CaMKIIa

    Available since

    April 18, 2012

    Availability

    Academic Institutions and Nonprofits only

    

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19. pAAV-CaMKIIa-hChR2(C128S/D156A)-EYFP

    Plasmid

    #35501

    Purpose

    AAV expression of CaMKII-driven stabilized step function opsin (SSFO) for optogenetic activation.

    Depositor

    Karl Deisseroth

    Article

    Yizhar et al Nature. 2011 Jul 27;477(7363):171-8. doi

    Insert

    hChR2
    

    Use

    AAV

    Tags

    EYFP

    Expression

    Mammalian

    Mutation

    C128S and D156A

    Promoter

    CaMKIIa

    Available since

    April 18, 2012

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

20. pAAV-CaMKIIa-hChR2(E123T/T159C)-EYFP

    Plasmid

    #35511

    Purpose

    AAV expression of CaMKIIa-driven hChR2 variant for ultrafast optogenetic control.

    Depositor

    Karl Deisseroth

    Article

    Mattis et al Nat Methods. 2011 Dec 18;9(2):159-72. do

    Insert

    hChR2
    

    Use

    AAV

    Tags

    EYFP

    Expression

    Mammalian

    Mutation

    E123T and T159C

    Promoter

    CaMKIIa

    Available since

    April 18, 2012

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge