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We narrowed to 19,528 results for: MUT

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Showing: 3141 - 3160 of 19528 results

  3. pcDNA3.1-V5H6-mPCSK3 D153A

    Plasmid
    #122681
    Purpose
    Expresses mutated mouse PCSK3 in mammalian cells (aspartic acid at position 153 mutated to alanine via site directed mutagenesis)
    Depositor
    Liming Pei
    Article
    Li et al Mol Cell Biol. 2018 Oct 15;38(21). pii:
    Insert
    Mus musculus furin (paired basic amino acid cleaving enzyme) (Furin), transcript variant 1 (Furin Mouse)
    Expression
    Mammalian
    Mutation
    changed Aspartic Acid 153 to Alanine
    Available Since
    May 16, 2019
    Availability
    Academic Institutions and Nonprofits only
    122681-plasmid-map-sequence-id-244746

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  5. pCMV6-AN-DDK_UBM1*2* POLI

    Plasmid
    #131232
    Purpose
    Mammalian expression of an N-terminally FLAG-tagged DNA polymerase iota mutant, containing point mutations in both UBM domains
    Depositor
    Roger Woodgate
    Article
    Ashton et al J Mol Biol. 2021 Jan 22;433(2):166733. d
    Insert
    DNA polymerase iota (POLI Human)
    Tags
    FLAG
    Expression
    Mammalian
    Mutation
    Contains inactivating mutations in both ubiquitin…
    Promoter
    CMV enhancer + CMV promoter
    Available Since
    Dec. 17, 2020
    Availability
    Academic Institutions and Nonprofits only
    131232-plasmid-map-sequence-id-267278

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  6. MLM3739

    Plasmid
    #49959
    Purpose
    Expresses a mutant TALE-TET1CD (inactive catalytic domain of TET1) fusion protein engineered to bind a site in the human beta-globin (HBB) gene for use as a control
    Depositor
    Keith Joung
    Article
    Maeder et al Nat Biotechnol. 2013 Oct 9. doi: 10.1038
    Insert
    3x Flag Tet1CDmut HB-4 (HBB Human)
    Use
    TALEN
    Tags
    3x FLAG
    Mutation
    catalytic domain of TET1 inactive (H1671Y and D16…
    Promoter
    EF1a
    Available Since
    Dec. 31, 2013
    Availability
    Academic Institutions and Nonprofits only

  10. pcDNA3-mCherry-TSC2(AA,3Q)-NLS

    Plasmid
    #192445
    Purpose
    Encodes mCherry-tagged TSC2 isoform 5 with S939A, T1439A, K1614Q, K1615Q, R1616Q mutations to disrupt Akt phosphorylation and GAP activity; targeted to the nucleus.
    Depositor
    Jin Zhang
    Article
    Zhong et al Cell Chem Biol. 2022 Jun 16;29(6):1037-1
    Insert
    mCherry-TSC2(S939A,T1439A,K1614Q,K1615Q,R1616Q )-NLS (TSC2 Human)
    Tags
    6xHIS - T7 tag (gene 10 leader) - Xpress (TM) tag…
    Expression
    Mammalian
    Mutation
    TSC2 Ser 939 and Thr 1439 (equivalent to 1462 in …
    Promoter
    CMV
    Available Since
    Dec. 7, 2022
    Availability
    Academic Institutions and Nonprofits only
    192445-plasmid-map-sequence-id-379137

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  11. pICE-HA-CtIP-siR-N289A-H290A

    Plasmid
    #82032
    Purpose
    Plasmid for constitutive or doxy-inducible expression of N289A+H290A mutant of human CtIP resistant to a siRNA. Confers resistance to puromycin. Use T-REx cells for doxycycline-inducible expression.
    Depositor
    Sébastien Britton, Patrick Calsou
    Article
    Chanut et al Nat Commun. 2016 Sep 19;7:12889. doi: 10
    Insert
    CtIP (RBBP8 Human)
    Tags
    HA
    Expression
    Mammalian
    Mutation
    Endonuclease-dead mutant: N289A+H290A CtIP; Mutat…
    Promoter
    CMV-tet
    Available Since
    Sept. 20, 2016
    Availability
    Academic Institutions and Nonprofits only
    82032-plasmid-map-sequence-id-155382

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  13. MLM3747

    Plasmid
    #49965
    Purpose
    Expresses a mutant TALE-TET1CD (inactive catalytic domain of TET1) fusion protein engineered to bind a site in the human beta-globin (HBB) gene for use as a control
    Depositor
    Keith Joung
    Article
    Maeder et al Nat Biotechnol. 2013 Oct 9. doi: 10.1038
    Insert
    3x Flag Tet1CDmut HB-6 (HBB Human)
    Use
    TALEN
    Tags
    3x FLAG
    Mutation
    catalytic domain of TET1 inactive (H1671Y and D16…
    Promoter
    EF1a
    Available Since
    Dec. 31, 2013
    Availability
    Academic Institutions and Nonprofits only

  14. MLM3743

    Plasmid
    #49962
    Purpose
    Expresses a mutant TALE-TET1CD (inactive catalytic domain of TET1) fusion protein engineered to bind a site in the human beta-globin (HBB) gene for use as a control
    Depositor
    Keith Joung
    Article
    Maeder et al Nat Biotechnol. 2013 Oct 9. doi: 10.1038
    Insert
    3x Flag Tet1CDmut HB-5 (HBB Human)
    Use
    TALEN
    Tags
    3x FLAG
    Mutation
    catalytic domain of TET1 inactive (H1671Y and D16…
    Promoter
    EF1a
    Available Since
    Dec. 31, 2013
    Availability
    Academic Institutions and Nonprofits only

  16. sgSf3b1(T1)

    Plasmid
    #90424
    Purpose
    induces dsDNA break within mouse Sf3b1 gene for subsequent homology-directed recombination and coding sequence mutagenesis at codon 700
    Depositor
    Alex Minella
    Article
    Gupta et al Nucleic Acids Res. 2018 Nov 20. pii: 519
    Insert
    mus Sf3b1 gRNA (Sf3b1 Mouse)
    Use
    CRISPR; Guiderna encoding for gene editing
    Expression
    Mammalian
    Promoter
    U6
    Available Since
    Nov. 26, 2018
    Availability
    Academic Institutions and Nonprofits only
    90424-plasmid-map-sequence-id-190772

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  17. pTE3328

    Plasmid
    #107539
    Purpose
    Expresses As crRNA and human codon optimized AsCpf1(RR mutant) in mammalian cells.
    Depositor
    Ervin Welker
    Article
    Toth et al Nucleic Acids Res. 2018 Sep 20. pii: 510
    Inserts
    As crRNA
    hAsCpf1(RR mutant)
    Tags
    3xHA, NLS, and SV40 NLS
    Expression
    Mammalian
    Mutation
    S542R, K607R
    Promoter
    CMV and human U6
    Available Since
    March 29, 2018
    Availability
    Academic Institutions and Nonprofits only
    107539-plasmid-map-sequence-id-207236

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  18. pTE3329

    Plasmid
    #107538
    Purpose
    Expresses Lb crRNA and human codon optimized LbCpf1(RR mutant) in mammalian cells.
    Depositor
    Ervin Welker
    Article
    Toth et al Nucleic Acids Res. 2018 Sep 20. pii: 510
    Inserts
    Lb crRNA
    hLbCpf1(RR mutant)
    Tags
    3xHA, NLS, and SV40 NLS
    Expression
    Mammalian
    Mutation
    G532R, K595R
    Promoter
    CMV and human U6
    Available Since
    March 29, 2018
    Availability
    Academic Institutions and Nonprofits only
    107538-plasmid-map-sequence-id-207235

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Showing: 3141 - 3160 of 19528 results