We narrowed to 166,040 results for: addgene

1. pSADdeltaG-GFP-ER(T2)CreER(T2)

   Plasmid

   #32649

   Depositor

   Edward Callaway

   Article

   Osakada et al Neuron. 2011 Aug 25;71(4):617-31.

   Inserts

   GFP
   ER(T2)CreER(T2)
   

   Use

   Rabies virus

   Expression

   Mammalian

   Promoter

   CMV

   Available Since

   Jan. 13, 2012

   Availability

   Academic Institutions and Nonprofits only

2. TET-pLKO.1 PURO shGFP

   Plasmid

   #83085

   Purpose

   Lentiviral shRNA vector for inducible knockdown of GFP (control hairpin)

   Depositor

   Kevin Janes

   Article

   Bajikar et al Dev Cell. 2017 Nov 20;43(4):418-435.e13.

   Insert

   shGFP
   

   Use

   Lentiviral

   Expression

   Mammalian

   Available Since

   Feb. 28, 2019

   Availability

   Academic Institutions and Nonprofits only

3. VV246: CoChR(C108S)-GCaMP6f-ER in fck

   Plasmid

   #58521

   Purpose

   Expresses CoChR(C108S) fused to GCaMP6f in mammalian cells

   Depositor

   Adam Cohen

   Article

   Venkatachalam et al Biophys J. 2014 Oct 7;107(7):1554-63. do

   Insert

   CoChR(C108S)-GCaMP6f-ER
   

   Use

   Lentiviral

   Tags

   GCaMP6f

   Expression

   Mammalian

   Mutation

   changed Cysteine 108 to Serine

   Promoter

   a-CamKII

   Available Since

   Nov. 5, 2014

   Availability

   Academic Institutions and Nonprofits only

   

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4. TET-pLKO.1 PURO shLacZ

   Plasmid

   #98632

   Purpose

   Lentiviral shRNA vector for inducible knockdown of LacZ (control hairpin)

   Depositor

   Kevin Janes

   Article

   Bajikar et al Dev Cell. 2017 Nov 20;43(4):418-435.e13.

   Insert

   shLacZ
   

   Use

   Lentiviral

   Expression

   Mammalian

   Available Since

   July 21, 2017

   Availability

   Academic Institutions and Nonprofits only

   

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5. pICH-chl-roGFP2-ORP1

   Plasmid

   #191699

   Purpose

   Ti plasmid pICH86988 harbouring chl-roGFP2-ORP1 for peroxide detection in the plastids of plant cells.

   Depositor

   Philip Mullineaux

   Article

   Exposito-Rodriguez et al bioRxiv 2024.01.18.576236

   Insert

   chl-roGFP2-ORP1
   

   Use

   Binary agrobacterium ti plasmid

   Expression

   Plant

   Promoter

   CaMV 35S promoter

   Available Since

   March 10, 2023

   Availability

   Academic Institutions and Nonprofits only

   

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6. pBigTB-FlpERT2

   Plasmid

   #149435

   Purpose

   tamoxifen-inducible Flp recombinase

   Depositor

   Primo Schaer

   Article

   Schwarz et al F1000Res. 2020 Sep 9;9:1112. doi: 10.126

   Insert

   Flp-ERT2 fusion protein
   

   Use

   Cre/Lox and Mouse Targeting

   Tags

   ERT2

   Expression

   Mammalian

   Promoter

   CAG promoter

   Available Since

   Sept. 10, 2020

   Availability

   Academic Institutions and Nonprofits only

   

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7. U6::sgBFP-U6::sgKrasG12D-pX333-red

   Plasmid

   #149448

   Purpose

   pX333 backbone expressing 2 sgRNA targeting BFP and KRAS G12D to edit mouse KRAS G12D allele.

   Depositor

   Jonathan Chernoff

   Article

   Budagyan et al Methods Mol Biol. 2021;2262:323-334. doi

   Insert

   sgRNA BFP and mouse KRAS G12D
   

   Use

   CRISPR

   Expression

   Mammalian

   Available Since

   Aug. 9, 2022

   Availability

   Academic Institutions and Nonprofits only

   

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8. pENTR1A_no_ccDB_mUty

   Plasmid

   #158570

   Purpose

   V5-tagged sequence of mUty cloned into entry vector for recombination

   Depositor

   Christian Deschepper

   Article

   Deschepper et al Sci Rep. 2020 Sep 10;10(1):14900. doi: 1

   Insert

   Mus musculus ubiquitously transcribed tetratricopeptide repeat containing, Y-linked (Uty), transcript variant 2 (Uty Mouse)
   

   Use

   Lentiviral

   Tags

   V5

   Available Since

   Sept. 21, 2020

   Availability

   Academic Institutions and Nonprofits only

   

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9. pKLV2-EF1a-NucGFP

   Plasmid

   #184696

   Purpose

   Lentiviral transfer plasmid for expression of NucGFP

   Depositor

   Brian Van Tine

   Article

   Rogers et al Cancer Metab. 2021 Jan 21;9(1):4. doi: 1

   Insert

   NucGFP
   

   Use

   Lentiviral

   Expression

   Mammalian

   Promoter

   EF1a

   Available Since

   Oct. 26, 2022

   Availability

   Academic Institutions and Nonprofits only

   

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10. pCAGEN-SBP-DICER1

    Plasmid

    #50558

    Purpose

    purification of Dicer1 from mammalian cells

    Depositor

    Yukihide Tomari

    Article

    Betancur et al RNA. 2012 Jan;18(1):24-30. doi: 10.1261/

    Insert

    Dicer1 (Dicer1 Mouse)
    

    Tags

    SBP

    Expression

    Mammalian

    Promoter

    CAG

    Available Since

    Jan. 3, 2014

    Availability

    Academic Institutions and Nonprofits only

11. pSLIK 3xFLAG-MTUS1 zeo

    Plasmid

    #192323

    Purpose

    Lentiviral expression vector for an inducible 3xFLAG-MTUS1

    Depositor

    Kevin Janes

    Article

    Wang et al Nat Commun. 2023 Apr 13;14(1):2110. doi:

    Insert

    MTUS1 (MTUS1 Human)
    

    Use

    Lentiviral

    Tags

    3xFLAG

    Available Since

    April 28, 2023

    Availability

    Academic Institutions and Nonprofits only

    

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12. pBabe-puro-miR-10b sponge

    Plasmid

    #25043

    Depositor

    Bob Weinberg

    Article

    Ma et al Nat Biotechnol. 2010 Apr . 28(4):341-7.

    Insert

    miR-10b sponge
    

    Use

    Retroviral

    Expression

    Mammalian

    Available Since

    June 11, 2010

    Availability

    Academic Institutions and Nonprofits only

13. TAL2385

    Plasmid

    #36794

    Depositor

    Keith Joung

    Article

    Reyon et al Nat Biotechnol. 2012 Apr 8. doi: 10.1038

    Insert

    BRCA1-TALEN_Right (Brca1 Human)
    

    Use

    Mammalian, t7 for in vitro mrna synthesis

    Tags

    3X Flag and WT FOKI

    Promoter

    CMV

    Available Since

    June 26, 2012

    Availability

    Academic Institutions and Nonprofits only

14. pIS1 Cyclin D2 long UTR

    Plasmid

    #21645

    Depositor

    David Bartel

    Article

    Mayr et al Cell. 2009 Aug 21. 138(4):673-84.

    Insert

    Cyclin D2 long UTR (CCND2 Human)
    

    Use

    Luciferase

    Tags

    Luciferase

    Expression

    Mammalian

    Available Since

    July 22, 2009

    Availability

    Academic Institutions and Nonprofits only

    

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15. pU6-sgRNA EF1Alpha-puro-T2A-BFP.ERH1

    Plasmid

    #164800

    Purpose

    For CRISPRi knockdown of ERH by lentiviral delivery of ERH gRNA1

    Depositor

    David Bartel

    Article

    Fang et al Mol Cell. 2020 Apr 16;78(2):289-302.e6.

    Inserts

    ERH CRISPRi gRNA1
    puro-T2A-BFP
    

    Use

    CRISPR and Lentiviral

    Expression

    Mammalian

    Promoter

    EF1Alpha and U6

    Available Since

    March 15, 2021

    Availability

    Academic Institutions and Nonprofits only

    

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16. pU6-sgRNA EF1Alpha-puro-T2A-BFP.ERH2

    Plasmid

    #164801

    Purpose

    For CRISPRi knockdown of ERH by lentiviral delivery of ERH gRNA2

    Depositor

    David Bartel

    Article

    Fang et al Mol Cell. 2020 Apr 16;78(2):289-302.e6.

    Inserts

    ERH CRISPRi gRNA2
    puro-T2A-BFP
    

    Use

    CRISPR and Lentiviral

    Expression

    Mammalian

    Promoter

    EF1Alpha and U6

    Available Since

    March 15, 2021

    Availability

    Academic Institutions and Nonprofits only

    

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17. Dopey2_pCSdest

    Plasmid

    #53850

    Depositor

    Roger Reeves

    Article

    Edie et al G3 (Bethesda). 2018 Jul 2;8(7):2215-2223

    Insert

    Dopey2
    

    Expression

    Mammalian

    Promoter

    SP6 and CMV

    Available Since

    Sept. 8, 2014

    Availability

    Industry, Academic Institutions, and Nonprofits

18. LoriT

    Plasmid

    #163691

    Purpose

    Modified L4440 containing now CamR and oriT for conjugation

    Depositor

    Baris Tursun

    Article

    Kazmierczak et al Nucleic Acids Res. 2021 Feb 26;49(4):e22

    Insert

    oriT
    

    Expression

    Bacterial

    Available Since

    Oct. 4, 2022

    Availability

    Academic Institutions and Nonprofits only

    

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19. pLentiCRISPRv1.mir451_KO

    Plasmid

    #164689

    Purpose

    For CRISPR knockout of miR-451 by lentiviral delivery of Cas9 and miR-451 gRNA

    Depositor

    David Bartel

    Article

    Fang et al Mol Cell. 2020 Apr 16;78(2):289-302.e6.

    Insert

    miR-451 CRISPR KO gRNA (MIR451A )
    

    Use

    CRISPR and Lentiviral

    Expression

    Mammalian

    Promoter

    U6

    Available Since

    March 15, 2021

    Availability

    Academic Institutions and Nonprofits only

    

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20. pCRUSH

    Plasmid

    #54480

    Purpose

    Mouse genomic ROSA26 targeting vector, expresses EGFP, cre/lox conditional U6 promoter pgk-puro

    Depositor

    Laurie Jackson-Grusby

    Article

    Brown et al Genesis. 2014 Jan;52(1):39-48. doi: 10.1

    Type

    Empty backbone

    Use

    Cre/Lox, Mouse Targeting, and RNAi

    Tags

    SA-EGFP-polyA, U6 promoter, loxP, and pgk-puro

    Expression

    Mammalian

    Promoter

    U6

    Available Since

    Aug. 18, 2014

    Availability

    Academic Institutions and Nonprofits only