We narrowed to 2,474 results for: MBP

1. p2CT-His-MBP-Lse_C2c2_WT

   Plasmid

   #83486

   Purpose

   Bacterial expression of WT L. seeligeri C2c2 CRISPR effector

   Depositor

   Jennifer Doudna

   Article

   East-Seletsky et al Nature. 2016 Sep 26. doi: 10.1038/nature

   Insert

   Lse_C2c2_WT
   

   Use

   CRISPR

   Tags

   His6-MBP

   Expression

   Bacterial

   Mutation

   Promoter

   Available since

   Nov. 1, 2016

   Availability

   Academic Institutions and Nonprofits only

   

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2. pET41-MBP-t-A3A-t-His

   Plasmid

   #109231

   Purpose

   Bacterial expression of A3A with N-terminal MBP an C-terminal His tag, both cleavable with TEV protease

   Depositor

   Rahul Kohli

   Article

   Schutsky et al Nat Biotechnol. 2018 Oct 8. pii: nbt.420

   Insert

   human APOBEC3A (APOBEC3A Human, Optimized for E. coli expression)
   

   Use

   Tags

   His and MBP

   Expression

   Bacterial

   Mutation

   Promoter

   Available since

   Oct. 16, 2018

   Availability

   Academic Institutions and Nonprofits only

   

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3. p2CT-His-MBP-Lsh_C2c2_WT

   Plasmid

   #83487

   Purpose

   Bacterial expression of WT L. shahii C2c2 CRISPR effector

   Depositor

   Jennifer Doudna

   Article

   East-Seletsky et al Nature. 2016 Sep 26. doi: 10.1038/nature

   Insert

   Lsh_C2c2_WT
   

   Use

   CRISPR

   Tags

   His6-MBP

   Expression

   Bacterial

   Mutation

   Promoter

   Available since

   Oct. 31, 2016

   Availability

   Academic Institutions and Nonprofits only

   

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4. pCm C-term MBP NikJC199U

   Plasmid

   #174365

   Purpose

   Expression plasmid with a TEV removable C-term 8xHis tagged MBP tag, and Chloramphenicol resistance. NikJ C199U cloned in the cloning site.

   Depositor

   Brandon Greene

   Article

   Caceres et al Methods Enzymol. 2022;662:119-141. doi:

   Insert

   nikJ, nikkomycin biosynthesis protein P1 [Streptomyces tendae]
   

   Use

   Tags

   8xHis, MBP, and TEV

   Expression

   Bacterial

   Mutation

   C199U

   Promoter

   T7

   Available since

   Dec. 7, 2021

   Availability

   Academic Institutions and Nonprofits only

   

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5. pRG/III-hs-MBP-CB2-HF

   Plasmid

   #121682

   Purpose

   Bacterial expression of cannabinoid receptor CB2 fusion protein

   Depositor

   Richard Henderson, Reinhard Grisshammer

   Article

   Calandra et al Biotechnology Letters

   Insert

   peripheral cannabinoid receptor (CNR2 Human)
   

   Use

   Tags

   His6 Flag tag

   Expression

   Bacterial

   Mutation

   Promoter

   lac

   Available since

   March 5, 2019

   Availability

   Academic Institutions and Nonprofits only

   

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6. p2CT-His-MBP-Lbu_C2c2_R472A_H477A_R1048A_H1053A

   Plasmid

   #83485

   Purpose

   Bacterial expression of L. buccalis C2c2 CRISPR effector (double HEPN nuclease 1 and 2 inactive mutant).

   Depositor

   Jennifer Doudna

   Article

   East-Seletsky et al Nature. 2016 Sep 26. doi: 10.1038/nature

   Insert

   Lbu_C2c2_R472A_H477A_R1048A_H1053A
   

   Use

   CRISPR

   Tags

   His6-MBP

   Expression

   Bacterial

   Mutation

   R472A, H477A, R1048A, H1053A

   Promoter

   Available since

   Oct. 31, 2016

   Availability

   Academic Institutions and Nonprofits only

   

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7. pET-MBP-NLS-Geo_st

   Plasmid

   #87703

   Purpose

   Expression plasmid for Cas9 from Geobacillus stearothermophilus with an N-Term MBP and SV40 NLS

   Depositor

   Jennifer Doudna

   Insert

   GeoCas9
   

   Use

   Tags

   10xHis-MBP-TEV

   Expression

   Bacterial

   Mutation

   Promoter

   Available since

   June 5, 2017

   Availability

   Academic Institutions and Nonprofits only

   

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8. p2CT-His-MBP-Lba_Cas13a_WT

   Plasmid

   #91867

   Purpose

   Bacterial expression for Cas13a

   Depositor

   Jennifer Doudna

   Article

   East-Seletsky et al Mol Cell. 2017 May 4;66(3):373-383.e3. d

   Insert

   LbaCas13a (LbaC2c2) WT
   

   Use

   Tags

   His6-MBP-TEV site

   Expression

   Bacterial

   Mutation

   Promoter

   Available since

   June 23, 2017

   Availability

   Academic Institutions and Nonprofits only

   

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9. pET28a-MBP-CBX1-eReader

   Plasmid

   #135089

   Purpose

   Bacterial expression of engineered CBX1 with enhanced binding to H3K9me

   Depositor

   Marcey Waters

   Article

   Albanese et al ACS Chem Biol. 2020 Jan 17;15(1):103-111

   Insert

   CBX1 chromobox protein 1, engineered
   

   Use

   Tags

   6xhis-tag and MBP

   Expression

   Bacterial

   Mutation

   lysine 43 mutated to alanine; aspartic acid 59 mu…

   Promoter

   T7

   Available since

   March 15, 2022

   Availability

   Academic Institutions and Nonprofits only

   

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10. FLAG-MBP1-E322TAG-W340A.pcDNA3-k

    Plasmid

    #127404

    Purpose

    Expresses MBP in mammalian cells. Amber codon at AA# 322 as a noncanonical amino acid incorporation site.

    Depositor

    Sharona Gordon

    Article

    Gordon et al Elife. 2018 Jun 20;7. pii: 37248. doi: 1

    Insert

    Maltose Binding Protein
    

    Use

    Tags

    FLAG

    Expression

    Mammalian

    Mutation

    Amino acid mutations: E322 to Amber codon and W34…

    Promoter

    CMV

    Available since

    June 18, 2019

    Availability

    Academic Institutions and Nonprofits only

    

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11. pET20b-NLuc-MBP-6xHis

    Plasmid

    #135931

    Purpose

    Expresses NanoLuciferase in bacterial cells.

    Depositor

    Antonio Amelio

    Article

    Parag-Sharma et al ACS Synth Biol. 2019 Dec 13. doi: 10.102

    Insert

    NanoLuciferase
    

    Use

    Tags

    MBP-6xHis

    Expression

    Bacterial

    Mutation

    Promoter

    T7

    Available since

    Feb. 11, 2020

    Availability

    Academic Institutions and Nonprofits only

    

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12. MBP-TSF-TEVcs-ULK1

    Plasmid

    #171416

    Purpose

    For the purifcation of human ULK1 complex

    Depositor

    James Hurley

    Article

    Chang et al Sci Adv. 2021 Apr 23;7(17). pii: 7/17/ea

    Insert

    ULK1 (ULK1 Synthetic)
    

    Use

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    Available since

    July 13, 2021

    Availability

    Academic Institutions and Nonprofits only

    

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13. pET-21a(+)/MBP-His

    Plasmid

    #38006

    Depositor

    Michael J Fox Foundation MJFF

    Insert

    MBP (Maltose binding protein)
    

    Use

    Tags

    6xHIS

    Expression

    Bacterial

    Mutation

    Promoter

    Available since

    Oct. 1, 2012

    Availability

    Academic Institutions and Nonprofits only

14. 6-His-MBP-TEV-FnCpf1

    Plasmid

    #90094

    Purpose

    Bacterial expression plasmid for protein purification

    Depositor

    Feng Zhang

    Article

    Zetsche et al Cell. 2015 Sep 23. pii: S0092-8674(15)01

    Insert

    FnCpf1 (humanized)
    

    Use

    Tags

    3xHA, 6xHis, MBP, Nucleoplasmin NLS, and TEV site…

    Expression

    Bacterial

    Mutation

    Promoter

    T7

    Available since

    June 19, 2017

    Availability

    Academic Institutions and Nonprofits only

    

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15. pBAD-MBP-His-ACPs

    Plasmid

    #141140

    Purpose

    Expression of holo-[acyl-carrier-protein] synthase

    Depositor

    New England Biolabs

    Insert

    acpS
    

    Use

    Tags

    MBP

    Expression

    Bacterial

    Mutation

    Promoter

    Available since

    May 28, 2020

    Availability

    Industry, Academic Institutions, and Nonprofits

    

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16. pTrc99S-ssDsbA-MBP-4xDQNAT

    Plasmid

    #128398

    Purpose

    Periplasmic expression of E. coli MBP with C terminal 4xDQNAT glycosylation sites in E. coli

    Depositor

    Michael Jewett

    Article

    Stark et al Sci Adv. 2021 Feb 3;7(6). pii: 7/6/eabe9

    Insert

    Maltose binding protein
    

    Use

    Tags

    4xDQNAT glycosylation tag, 6xHis tag, and DsbA si…

    Expression

    Bacterial

    Mutation

    Y342H- please see depositor comments

    Promoter

    trc

    Available since

    April 13, 2021

    Availability

    Academic Institutions and Nonprofits only

    

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17. pSL0375 (His-MBP-TniQ_Cas8_Cas7_Cas6)

    Plasmid

    #130640

    Purpose

    Expresses V. cholerae CAST TniQ, Cas8, Cas7, and Cas6 from a T7 promoter, with N-terminal His10-MBP-TEVsite tag on TniQ for purification.

    Depositor

    Samuel H. Sternberg

    Article

    Klompe et al Nature. 2019 Jul;571(7764):219-225. doi:

    Insert

    VchTniQ, VchCas8, VchCas7, VchCas6
    

    Use

    CRISPR; Transposon

    Tags

    His10-MBP-TEVsite on TniQ

    Expression

    Bacterial

    Mutation

    Promoter

    T7

    Available since

    Sept. 4, 2019

    Availability

    Academic Institutions and Nonprofits only

    

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18. pDB-His-MBP-mGSDMD

    Plasmid

    #123365

    Purpose

    Expression of full-length mouse GSDMD in E. coli

    Depositor

    Hao Wu

    Article

    Orning et al Science. 2018 Nov 30;362(6418):1064-1069

    Insert

    GSDMD (Gsdmd Mouse)
    

    Use

    Tags

    His6-MBP

    Expression

    Bacterial

    Mutation

    Promoter

    T7

    Available since

    May 3, 2019

    Availability

    Academic Institutions and Nonprofits only

    

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19. pBAD-MBP-His-SFP

    Plasmid

    #141141

    Purpose

    Expression of 4'-phosphopantetheinyl transferase gene

    Depositor

    New England Biolabs

    Insert

    4'-phosphopantetheinyl transferase
    

    Use

    Tags

    MBP

    Expression

    Bacterial

    Mutation

    Promoter

    Available since

    May 28, 2020

    Availability

    Industry, Academic Institutions, and Nonprofits

    

    Enlarge

20. MBP-CHC(1-1074)-His6

    Plasmid

    #100748

    Purpose

    Bacterial expression of MBP-tagged clathrin heavy chain large fragment including N-terminal domain, his tagged at C-terminus for purification.

    Depositor

    Stephen Royle

    Article

    Wood et al J Cell Biol. 2017 Sep 27. pii: jcb.20170

    Insert

    CLTC (CLTC Human)
    

    Use

    Tags

    His and MBP

    Expression

    Bacterial

    Mutation

    Residues 1-1074

    Promoter

    Available since

    Sept. 12, 2017

    Availability

    Academic Institutions and Nonprofits only

    

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