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Enter a sequence to perform a BLAST-based search of Addgene plasmid sequence databases.

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Showing: 301 - 320 of 19522 results

  1. pBS-Hsp70-Cas9

    Plasmid
    #46294
    Purpose
    A codon-optimized Cas9 nuclease under the control of the Drosophila hsp70 promoter.
    Depositor
    Melissa Harrison, Kate O'Connor-Giles, Jill Wildonger
    Publication
    Gratz et al Genetics. 2014 Apr;196(4):961-71. doi: 1
    Insert
    codon optimized Cas9
    Use
    CRISPR
    Tags
    3x Flag
    Expression
    Insect
    Mutation
    Silent change from C to A at bp 834 of insert
    Promoter
    Hsp70
    Available since
    July 8, 2013
    Availability
    Academic Institutions and Nonprofits only
    Cited by
    36 Articles
    46294-plasmid-map-sequence-id-332831

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  9. CMV-mito-R-GECO1

    Plasmid
    #46021
    Purpose
    Red intensiometric genetically encoded Ca2+-indicators for optical imaging
    Depositor
    Robert Campbell
    Publication
    Wu et al ACS Chem Neurosci. 2013 Mar 19.
    Insert
    R-GECO1
    Tags
    a duplex of the mitochondrial targeting signal of…
    Expression
    Mammalian
    Mutation
    Substitutions relative to the mApple-derived anal…
    Promoter
    CMV
    Available since
    July 23, 2013
    Availability
    Academic Institutions and Nonprofits only
    Cited by
    22 Articles
    46021-plasmid-map-sequence-id-350822

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  18. pCAS

    Plasmid
    #60847
    Purpose
    Expresses S. pyogenes Cas9 plus an HDV ribozyme-sgRNA for genome editing in yeast
    Depositor
    Jamie Cate
    Publication
    Ryan et al Elife. 2014 Aug 19;3. doi: 10.7554/eLife
    Inserts
    S. pyogenese Cas9
    RNA pol III promoter (tRNA-Tyr)
    hepatitis delta virus ribozyme, genomic
    sgRNA
    Use
    CRISPR and Synthetic Biology
    Tags
    NLS/His8 and TTT 3' extension prior to sgRNA
    Expression
    Bacterial and Yeast
    Mutation
    L4 is UUCG tetraloop and guide targets LYP1 (CATA…
    Available since
    Jan. 13, 2015
    Availability
    Academic Institutions and Nonprofits only
    Cited by
    31 Articles
    60847-plasmid-map-sequence-id-197511

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  20. pAAV.Syn.Flex.NES-jRCaMP1b.WPRE.SV40 (AAV1)

    Viral prep
    #100850-AAV1
    Purpose
    Ready-to-use AAV1 particles produced from pAAV.Syn.Flex.NES-jRCaMP1b.WPRE.SV40 (#100850). In addition to the viral particles, you will also receive purified pAAV.Syn.Flex.NES-jRCaMP1b.WPRE.SV40 plasmid DNA. Syn-driven, Cre-dependent NES-jRCaMP1b calcium sensor. These AAV preparations are suitable purity for injection into animals.
    Depositor
    Douglas Kim, GENIE Project
    Publication
    Dana et al eLife. 2016 Mar 24;5. pii: e12727. doi:
    Promoter
    Syn
    Available since
    May 11, 2018
    Availability
    Academic Institutions and Nonprofits only
Showing: 301 - 320 of 19522 results