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CLYBL-TO-hNGN2-BSD-mApple

Plasmid

#124229

Purpose

Targets the human CLYBL safe harbor locus for integration of dox-inducible NGN2 for differentiation of iPSCs into cortical neurons. Contains a floxed BSD-NLS-mApple selection cassette.

Depositor

Michael Ward

Article

Tian et al Neuron. 2019 Aug 5. pii: S0896-6273(19)3

Insert

NGN2 (NEUROG2 Human)

Expression

Mammalian

Available Since

April 12, 2019

Availability

Academic Institutions and Nonprofits only

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pAAV-EF1a-mCherry-IRES-Flpo

Plasmid

#55634

Purpose

Expresses Flpo in Mammalian Cells

Depositor

Karl Deisseroth

Article

Fenno et al Nat Methods. 2014 Jul;11(7):763-72. doi:

Has Service

AAV Retrograde and AAV1

Inserts

mCherry
Flpo

Use

AAV

Expression

Mammalian

Promoter

Ef1a and Ef1a/IRES

Available Since

July 22, 2014

Availability

Academic Institutions and Nonprofits only

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pWZL Blast mouse E-cadherin

Plasmid

#18804

Depositor

Bob Weinberg

Article

Onder et al Cancer Res. 2008 May 15. 68(10):3645-54.

Insert

E-cadherin (Cdh1 Mouse)

Use

Retroviral

Expression

Mammalian

Available Since

July 7, 2008

Availability

Academic Institutions and Nonprofits only

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1136- Desmoplakin-GFP

Plasmid

#32227

Depositor

Kathleen Green

Article

Godsel et al J Cell Biol. 2005 Dec 19;171(6):1045-59.

Insert

Desmoplakin (DSP Human)

Tags

EGFP

Expression

Mammalian

Promoter

CMV

Available Since

Nov. 8, 2011

Availability

Academic Institutions and Nonprofits only

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pLV hUbC-VP64 dCas9 VP64-T2A-GFP

Plasmid

#59791

Purpose

Co-expresses human optimized S. pyogenes dCas9 fused to two copies of VP64 and GFP

Depositor

Charles Gersbach

Article

Kabadi et al Nucleic Acids Res. 2014 Aug 13. pii: gku

Insert

humanized VP64 dead Cas9 VP64 T2A GFP

Use

CRISPR and Lentiviral

Tags

Flag

Expression

Mammalian

Mutation

D10A and H840A

Available Since

Oct. 23, 2014

Availability

Academic Institutions and Nonprofits only

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pFA6a-link-yomRuby2-Kan

Plasmid

#44953

Purpose

Yeast tagging vector to create gene fusion with yeast optimized mRuby2 with Kan selection

Depositor

Wendell Lim, Kurt Thorn

Article

Lee et al PLoS One. 2013 Jul 2;8(7):e67902. doi: 1

Insert

yomRuby2

Expression

Yeast

Available Since

May 9, 2013

Availability

Academic Institutions and Nonprofits only

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pMA3790

Plasmid

#70110

Purpose

mtDNA elimination/production of rho-0 cells

Depositor

Mikhail Alexeyev

Article

Spadafora et al PLoS One. 2016 May 2;11(5):e0154684. doi

Inserts

EGFP
hUNG1 Y147A
Neo

Tags

None, human OTC mitochondrial targeting sequence,…

Expression

Mammalian

Mutation

Y147A, aa's 1-77 removed

Promoter

CMV, EF1a, and SV40 and native

Available Since

May 3, 2016

Availability

Academic Institutions and Nonprofits only

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pLV GG hUbC-dsRED

Plasmid

#84034

Purpose

Contains Golden Gate cassette to express up to four gRNAs and dsRED

Depositor

Charles Gersbach

Article

Kabadi et al Nucleic Acids Res. 2014 Aug 13. pii: gku

Type

Empty backbone

Use

CRISPR and Lentiviral

Expression

Mammalian

Available Since

Nov. 7, 2016

Availability

Academic Institutions and Nonprofits only

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pSECC

Plasmid

#60820

Purpose

3rd generation vector. Expresses a sgRNA of interest, Cas9 and Cre

Depositor

Tyler Jacks

Article

Sanchez-Rivera et al Nature. 2014 Oct 22. doi: 10.1038/nature

Inserts

Cas9
Cre

Use

CRISPR, Cre/Lox, Lentiviral, and Mouse Targeting

Tags

Flag

Expression

Mammalian

Mutation

BsmBI site eliminated by C->A; E308G

Promoter

EFS and EFS (after Cas9-2A)

Available Since

Nov. 19, 2014

Availability

Academic Institutions and Nonprofits only

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pLVX-EF1alpha-2xGFP:NES-IRES-2xRFP:NLS

Plasmid

#71396

Purpose

2Gi2R assay

Depositor

Fred Gage

Article

Mertens et al Cell Stem Cell. 2015 Oct 6. pii: S1934-5

Inserts

2xGFP:NES
2xRFP:NLS

Use

Lentiviral

Tags

IRES

Expression

Mammalian

Promoter

EF1alpha and IRES

Available Since

Dec. 14, 2015

Availability

Academic Institutions and Nonprofits only

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lenti U6-sgRNA/EF1a-mCherry

Plasmid

#114199

Purpose

Lentivirus compatible SpCas9/dCas9 sgRNA scaffold driven by the U6 promoter and mCherry driven by the EF1a promoter

Depositor

Jeremy Day

Article

Savell et al eNeuro. 2019 Mar 7;6(1). pii: eN-MNT-049

Insert

sgRNA scaffold

Use

Lentiviral

Tags

mCherry

Expression

Mammalian

Promoter

U6

Available Since

Sept. 7, 2018

Availability

Academic Institutions and Nonprofits only

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pSMPP-mCherry-hTRIM21

Plasmid

#104972

Purpose

Lentiviral Transfer Plasmid with mCherry-tagged human TRIM21

Depositor

Leo James

Article

Clift et al Cell. 2017 Nov 13. pii: S0092-8674(17)31

Insert

Tripartite Motif containing 21 (TRIM21 Human)

Use

Lentiviral

Tags

mCherry

Promoter

SFFV

Available Since

Feb. 6, 2018

Availability

Academic Institutions and Nonprofits only

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PBCAG-eGFP

Plasmid

#40973

Depositor

Joseph Loturco

Article

Chen et al J Neurosci Methods. 2012 Jun 15;207(2):1

Inserts

PiggyBac transposon 3' terminal repeats
PiggyBac 5' terminal repeats

Expression

Mammalian

Available Since

May 1, 2017

Availability

Academic Institutions and Nonprofits only

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pY094

Plasmid

#84743

Purpose

Expresses huAsCpf1-T2A-GFP and crRNA guide

Depositor

Feng Zhang

Article

Zetsche et al Nat Biotechnol. 2017 Jan;35(1):31-34. do

Inserts

huAsCpf1
GFP

Tags

3xHA, NLS, and T2A

Expression

Mammalian

Promoter

CMV

Available Since

Jan. 25, 2017

Availability

Academic Institutions and Nonprofits only

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Thy1 promoter construct

Plasmid

#20736

Depositor

Joshua Sanes

Article

Feng et al Neuron. 2000 Oct . 28(1):41-51.

Insert

Thy1 promoter (Thy1 Mouse)

Expression

Mammalian

Available Since

April 14, 2009

Availability

Academic Institutions and Nonprofits only

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pH3U3-mcs

Plasmid

#12609

Depositor

Scot Wolfe

Article

Meng et al Nat Biotechnol. 2005 Aug . 23(8):988-94.

Insert

Multiple cloning site in His3 Ura3 reporter plasmid

Expression

Bacterial

Available Since

Sept. 26, 2006

Availability

Academic Institutions and Nonprofits only

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LeGO-iG2

Plasmid

#27341

Depositor

Boris Fehse

Article

Weber et al Mol Ther. 2008 Apr . 16(4):698-706.

Insert

SFFV promoter, IRES-eGFP

Use

Lentiviral

Expression

Mammalian

Available Since

April 24, 2012

Availability

Academic Institutions and Nonprofits only

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pcDNA3.1-CMV-CFP;UBC-Cre25nt

Plasmid

#65727

Purpose

Expresses Cre recombinase with a 25 nucleotide extracellular vesicle targeting sequence under the UBC promotor and CFP under the CMV promotor.

Depositor

Jacco van Rheenen

Article

Zomer et al Cell. 2015 May 21;161(5):1046-57. doi: 1

Inserts

CMV promoter driving CFP expression
UBC promoter driving Cre-25nt expression

Use

Cre/Lox

Expression

Mammalian

Promoter

CMV and UBC

Available Since

July 6, 2015

Availability

Academic Institutions and Nonprofits only

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LentiV_Cas9_puro

Plasmid

#108100

Purpose

Lentiviral expression plasmid of spCas9 with puromycin resistance gene

Depositor

Christopher Vakoc

Article

Tarumoto et al Mol Cell. 2018 Feb 28. pii: S1097-2765(1

Insert

Cas9

Use

CRISPR and Lentiviral

Tags

FLAG

Expression

Mammalian

Promoter

EFS promoter

Available Since

July 5, 2018

Availability

Academic Institutions and Nonprofits only

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pBabe-c-myc-zeo

Plasmid

#17758

Depositor

Susan Lindquist

Article

Dai et al Cell. 2007 Sep 21. 130(6):1005-18.

Insert

c-myc (MYC Human)

Use

Retroviral

Expression

Mammalian

Available Since

April 14, 2008

Availability

Academic Institutions and Nonprofits only

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