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pWT062a

Plasmid

#107909

Purpose

W2m.2-2 plasmid. Contains UBC-TetR-P2A-LacI and is ppart of the CAMERA2m system for cellular recording in mammalian cells

Depositor

David Liu

Article

Tang et al Science. 2018 Feb 15. pii: science.aap89

Insert

UBC-TetR-P2A-LacI

Expression

Mammalian

Available Since

March 29, 2018

Availability

Academic Institutions and Nonprofits only

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pVG329

Plasmid

#227771

Purpose

For genomic replacement of YOR1 gene in budding yeast

Depositor

Sahand Jamal Rahi

Article

Gligorovski et al bioRxiv 2024.02.28.582517

Insert

Genomic sequences flanking YOR1 in budding yeast (YOR1 Budding Yeast)

Expression

Yeast

Mutation

N/A

Available Since

Jan. 15, 2025

Availability

Academic Institutions and Nonprofits only

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pFA6 - iFAST

Plasmid

#209414

Purpose

iFAST containing plasmid for endogenous fusion constructs

Depositor

Patrick Van Dijck

Article

Devos et al Microbiology (Reading). 2024 Mar;170(3).

Insert

iFAST

Expression

Yeast

Promoter

none

Available Since

April 3, 2024

Availability

Academic Institutions and Nonprofits only

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pWT007a

Plasmid

#107889

Purpose

W1.0.1 plasmid. Contains PTetO-SD8-Cas9, PLac-sgRNA1 and is part of CAMERA 1.0.1

Depositor

David Liu

Article

Tang et al Science. 2018 Feb 15. pii: science.aap89

Insert

PTetO-SD8-Cas9, PLac-sgRNA1

Expression

Bacterial

Available Since

Aug. 10, 2020

Availability

Academic Institutions and Nonprofits only

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pTK583

Plasmid

#114695

Purpose

SNAP-tagging at NuMA's C-terminal locus

Depositor

Tomomi Kiyomitsu

Article

Okumura et al Elife. 2018 May 31;7. pii: 36559. doi: 1

Insert

SNAP

Available Since

Oct. 16, 2018

Availability

Academic Institutions and Nonprofits only

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pWT047c

Plasmid

#107903

Purpose

W2.7-1 plasmid. Contains PR-sd5-BE2, PLac-sgRNA1 and is part of CAMERA 2.7

Depositor

David Liu

Article

Tang et al Science. 2018 Feb 15. pii: science.aap89

Insert

PR-sd5-BE2, PLac-sgRNA1

Expression

Bacterial

Available Since

April 17, 2018

Availability

Academic Institutions and Nonprofits only

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pWT045c

Plasmid

#107902

Purpose

W2.6 plasmid. Contains PTetO-SD8-BE2, PPSP-sgRNA1 and is part of CAMERA 2.6

Depositor

David Liu

Article

Tang et al Science. 2018 Feb 15. pii: science.aap89

Insert

PTetO-SD8-BE2, PPSP-sgRNA1

Expression

Bacterial

Available Since

April 17, 2018

Availability

Academic Institutions and Nonprofits only

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pWT007g

Plasmid

#107890

Purpose

W1.0.2 plasmid. Contains PTetO-sd2-Cas9, PLac-sgRNA1 and is part of CAMERA 1.0.2

Depositor

David Liu

Article

Tang et al Science. 2018 Feb 15. pii: science.aap89

Insert

PTetO-sd2-Cas9, PLac-sgRNA1

Expression

Bacterial

Available Since

April 17, 2018

Availability

Academic Institutions and Nonprofits only

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pT7RNAP

Plasmid

#170101

Purpose

expresses T7 RNAP

Depositor

Chase Beisel

Article

Wimmer et al Methods Mol Biol. 2022;2433:391-411. doi

Insert

T7-RNA polymerase

Use

Synthetic Biology

Expression

Bacterial

Promoter

p70a

Available Since

Jan. 27, 2022

Availability

Academic Institutions and Nonprofits only

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pJPC12-ΔM13-PRM-B0034-geneVI

Plasmid

#80858

Purpose

Accessory plasmid (encodes M13 gene VI)

Depositor

Mark Isalan

Article

Brodel et al Nat Commun. 2016 Dec 16;7:13858. doi: 10

Insert

M13 gene VI

Expression

Bacterial

Promoter

Lambda Prm

Available Since

Dec. 19, 2016

Availability

Academic Institutions and Nonprofits only

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pTK511

Plasmid

#114686

Purpose

Express Mem-BFP-iLID

Depositor

Tomomi Kiyomitsu

Article

Okumura et al Elife. 2018 May 31;7. pii: 36559. doi: 1

Insert

Mem-BFP-iLID

Available Since

April 1, 2019

Availability

Academic Institutions and Nonprofits only

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pJPC12-ΔM13-mCherry-PR/PRM-GFP

Plasmid

#80859

Purpose

Reporter plasmid (mCherry and GFP)

Depositor

Mark Isalan

Article

Brodel et al Nat Commun. 2016 Dec 16;7:13858. doi: 10

Inserts

mCherry
GFP

Expression

Bacterial

Promoter

Lambda Pr and Lambda Prm

Available Since

Dec. 19, 2016

Availability

Academic Institutions and Nonprofits only

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pTK630

Plasmid

#114697

Purpose

Express RFP-Nano from Rosa 26 locus

Depositor

Tomomi Kiyomitsu

Article

Okumura et al Elife. 2018 May 31;7. pii: 36559. doi: 1

Insert

RFP-Nano

Available Since

Oct. 16, 2018

Availability

Academic Institutions and Nonprofits only

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pJethlpAmScarletCm

Plasmid

#206818

Purpose

Template for hlpA-mScarlet-I amplification associated with chloramphenicol resistance gene

Depositor

Virginie Libante

Article

Mignolet et al bioRxiv 2025.05.10.653270

Insert

hlpA

Use

Synthetic Biology

Tags

mScarlet

Available Since

May 12, 2025

Availability

Academic Institutions and Nonprofits only

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pJethlpAYFPCm

Plasmid

#206826

Purpose

Template for hlpA-YFP amplification associated with chloramphenicol resistance gene

Depositor

Virginie Libante

Article

Mignolet et al bioRxiv 2025.05.10.653270

Insert

hlpA

Use

Synthetic Biology

Tags

YFP

Available Since

May 12, 2025

Availability

Academic Institutions and Nonprofits only

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pJethlpAmNeonGreenCm

Plasmid

#206814

Purpose

Template for hlpA-mneongreen amplification associated with chloramphenicol resistance gene

Depositor

Virginie Libante

Article

Mignolet et al bioRxiv 2025.05.10.653270

Insert

hlpA

Use

Synthetic Biology

Tags

mNeonGreen

Mutation

3insG

Available Since

May 12, 2025

Availability

Academic Institutions and Nonprofits only

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pJethlpAmNeonGreenKm

Plasmid

#206816

Purpose

Template for hlpA-mneongreen amplification associated with kanamycin resistance gene

Depositor

Virginie Libante

Article

Mignolet et al bioRxiv 2025.05.10.653270

Insert

hlpA

Use

Synthetic Biology

Tags

mNeonGreen

Available Since

May 12, 2025

Availability

Academic Institutions and Nonprofits only

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pTK750

Plasmid

#114718

Purpose

Express mCherry-NuMA(5A-3) from Rosa 26 locus

Depositor

Tomomi Kiyomitsu

Article

Okumura et al Elife. 2018 May 31;7. pii: 36559. doi: 1

Insert

NuMA 5A-3

Available Since

April 11, 2019

Availability

Academic Institutions and Nonprofits only

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pTK586

Plasmid

#114716

Purpose

SNAP-tagging at LGN's N-terminal locus (BSD)

Depositor

Tomomi Kiyomitsu

Article

Okumura et al Elife. 2018 May 31;7. pii: 36559. doi: 1

Insert

SNAP

Available Since

Oct. 18, 2018

Availability

Academic Institutions and Nonprofits only

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pTK473

Plasmid

#114715

Purpose

sgRNA for LGN's N-terminal locus

Depositor

Tomomi Kiyomitsu

Article

Okumura et al Elife. 2018 May 31;7. pii: 36559. doi: 1

Insert

LGN sgRNA

Available Since

Oct. 17, 2018

Availability

Academic Institutions and Nonprofits only

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