We narrowed to 10,398 results for: 158

1. pSSRB274_pAC8-eGFP-hsCRBN

   Plasmid

   #218791

   Purpose

   Insect cell expression vector for FLAG-TEV-eGFP-Prescission-hsCRBN

   Depositor

   Eric Fischer

   Article

   Mercer et al Science. 2024 Mar 15;383(6688):eadk4422.

   Insert

   Protein cereblon (CRBN Human)
   

   Use

   Tags

   FLAG-TEV-eGFP-Prescission

   Expression

   Insect

   Mutation

   eGFP fused to N-terminus

   Promoter

   polyhedrin

   Available since

   May 6, 2024

   Availability

   Academic Institutions and Nonprofits only

   

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2. Target-AID (pRZ762)

   Plasmid

   #131300

   Purpose

   CAG promoter expression plasmid for NLS-hSpCas9n(D10A)-NLS-SH3-3xFLAG-pmCDA1(R187W with reference to NCBI ABO15149.1)-NLS-UGI-P2A-EGFP.

   Depositor

   Keith Joung

   Article

   Grunewald et al Nat Biotechnol. 2019 Sep 2. pii: 10.1038

   Insert

   NLS-hSpCas9n(D10A)-NLS-SH3-3xFLAG-pmCDA1(R187W with reference to NCBI ABO15149.1)-NLS-UGI-P2A-EGFP
   

   Use

   Tags

   Expression

   Mammalian

   Mutation

   Promoter

   CAG

   Available since

   Oct. 24, 2019

   Availability

   Academic Institutions and Nonprofits only

   

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3. pPV-TetO-SpCas9-GR-iC-ERiP (CRONUS-Puro)

   Plasmid

   #100596

   Purpose

   piggyBac vector expressing Dual-regulated Cas9 (Puro resistance)

   Depositor

   Akitsu Hotta

   Article

   Ishida et al Sci Rep. 2018 Jan 10;8(1):310. doi: 10.1

   Inserts

   CRISPR Cas9 fused with human Glucocorticoid Receptor
   mCherry
   rtTA-M2
   

   Use

   Piggybac vector

   Tags

   Expression

   Mammalian

   Mutation

   Codon-optimized for human codon usage

   Promoter

   Dox-inducible TetO promoter and Human EEF1A1 prom…

   Available since

   April 2, 2018

   Availability

   Academic Institutions and Nonprofits only

   

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4. pcDNA5/FRT/TO-GAlphaGustducin-RLuc8

   Plasmid

   #140979

   Purpose

   Encodes a G alpha subunit (GNAT3) containing RLuc8 as an optimal component of a BRET2 biosensor for studying heterotrimeric G protein dissociation

   Depositor

   Bryan Roth

   Article

   Olsen et al Nat Chem Biol. 2020 May 4. pii: 10.1038/

   Insert

   GAlphaGustducin-RLuc8 (GNAT3 Human)
   

   Use

   Luciferase

   Tags

   Expression

   Mammalian

   Mutation

   RLuc8 and flanking SGGGGS linkers have been inser…

   Promoter

   CMV

   Available since

   June 26, 2020

   Availability

   Academic Institutions and Nonprofits only

   

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5. pCAG-Archon2-KGC-GFP-ER2-WPRE

   Plasmid

   #108424

   Purpose

   Archon2 fluorescent voltage reporter in mammalian expression plasmid

   Depositor

   Edward Boyden

   Article

   Piatkevich et al Nat Chem Biol. 2018 Feb 26. pii: 10.1038

   Insert

   Archon2-KGC-EGFP-ER2
   

   Use

   Tags

   Expression

   Mammalian

   Mutation

   Promoter

   CAG

   Available since

   April 10, 2018

   Availability

   Academic Institutions and Nonprofits only

   

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6. pmiRFP-N1

   Plasmid

   #108409

   Purpose

   Monomeric near-infrared fluorescent protein miRFP in mammalian expression plasmid

   Depositor

   Edward Boyden

   Article

   Piatkevich et al Nat Chem Biol. 2018 Feb 26. pii: 10.1038

   Insert

   miRFP
   

   Use

   Tags

   Expression

   Mammalian

   Mutation

   Promoter

   CMV

   Available since

   April 10, 2018

   Availability

   Academic Institutions and Nonprofits only

   

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7. pIG-703pUC19-tNGFR-P2A-BACH2

   Plasmid

   #186112

   Purpose

   Knockin of truncated NGFR to target gene

   Depositor

   Alexander Marson

   Article

   Shy et al Nat Biotechnol. 2022 Aug 25:10.1038/s415

   Insert

   BACH2 (BACH2 Human)
   

   Use

   CRISPR

   Tags

   Expression

   Mutation

   WT with tNGFR sequence

   Promoter

   Available since

   July 28, 2022

   Availability

   Academic Institutions and Nonprofits only

   

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8. pTol2-UAS-zmiRFP

   Plasmid

   #108415

   Purpose

   Codon-optimized monomeric near-infrared fluorescent protein miRFP in zebrafish expression plasmid

   Depositor

   Edward Boyden

   Article

   Piatkevich et al Nat Chem Biol. 2018 Feb 26. pii: 10.1038

   Insert

   zmiRFP
   

   Use

   Zebrafish

   Tags

   Expression

   Mutation

   Promoter

   UAS

   Available since

   April 10, 2018

   Availability

   Academic Institutions and Nonprofits only

   

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9. pTU1-A-pdh_RiboJ_GFP+_MGApt_Bba_B0015

   Plasmid

   #107576

   Purpose

   B. megaterium DSM319 pdh promoter, GFP+ with malachite green mRNA aptamer in Bacillus cell-free transcription-translation. Bacillus shuttle vector backbone, colE1/AmpR (E. coli), RebB/TetA (Bacillus)

   Depositor

   Paul Freemont

   Article

   Moore et al Proc Natl Acad Sci U S A. 2018 Apr 17. p

   Insert

   GFP+
   

   Use

   Synthetic Biology

   Tags

   B. megaterium DSM319 xylA leader sequence (MTSSKI…

   Expression

   Bacterial

   Mutation

   F64L/ S65T/ Q80R/ F99S/ M153T/ V163A

   Promoter

   pdh promoter Bacillus megaterium DSM319

   Available since

   July 3, 2018

   Availability

   Academic Institutions and Nonprofits only

   

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10. pHR-EF1a-Archon2-KGC-EGFP-ER2

    Plasmid

    #108426

    Purpose

    Lentivirus production plasmid encoding for Archon2 fluorescent voltage reporter

    Depositor

    Edward Boyden

    Article

    Piatkevich et al Nat Chem Biol. 2018 Feb 26. pii: 10.1038

    Insert

    Archon2-KGC-EGFP-ER2
    

    Use

    Lentiviral

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    EF1a

    Available since

    April 10, 2018

    Availability

    Academic Institutions and Nonprofits only

    

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11. pBAD-HisD-miRFP

    Plasmid

    #108410

    Purpose

    Monomeric near-infrared fluorescent protein miRFP in bacterial expression plasmid

    Depositor

    Edward Boyden

    Article

    Piatkevich et al Nat Chem Biol. 2018 Feb 26. pii: 10.1038

    Insert

    miRFP
    

    Use

    Tags

    Expression

    Bacterial

    Mutation

    Promoter

    araB

    Available since

    April 10, 2018

    Availability

    Academic Institutions and Nonprofits only

    

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12. pTR-177 pUC19-CLTA-N-mCherry

    Plasmid

    #186128

    Purpose

    knockin of mCherry to CLTA target

    Depositor

    Alexander Marson

    Article

    Shy et al Nat Biotechnol. 2022 Aug 25:10.1038/s415

    Insert

    CLTA (CLTA Human)
    

    Use

    CRISPR

    Tags

    Expression

    Mutation

    WT with mCherry sequence

    Promoter

    Available since

    July 28, 2022

    Availability

    Academic Institutions and Nonprofits only

    

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13. pSpCas9(BB)-2A-Neo_h53BP1_gRNA_D

    Plasmid

    #110300

    Purpose

    Expresssion of Cas9-T2A-neomycin resistant gene and a gRNA targeting exon 10 of human 53BP1

    Depositor

    Chris Kok-Lung Chan

    Article

    Addis Jones et al Nat Commun. 2019 Jun 28;10(1):2861. doi:

    Insert

    p53-binding protein 1 (TP53BP1 Human)
    

    Use

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    Available since

    Feb. 22, 2021

    Availability

    Academic Institutions and Nonprofits only

    

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14. pTR-469 JUNB-tNGFR

    Plasmid

    #186060

    Purpose

    Knockin of truncated NGFR to target gene

    Depositor

    Alexander Marson

    Article

    Shy et al Nat Biotechnol. 2022 Aug 25:10.1038/s415

    Insert

    JUNB (JUNB Human)
    

    Use

    CRISPR

    Tags

    Expression

    Mutation

    WT with tNGFR sequence

    Promoter

    Available since

    July 28, 2022

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

15. pTR-472 FOXO1-tNGFR

    Plasmid

    #186062

    Purpose

    Knockin of truncated NGFR to target gene

    Depositor

    Alexander Marson

    Article

    Shy et al Nat Biotechnol. 2022 Aug 25:10.1038/s415

    Insert

    FOXO1 (FOXO1 Human)
    

    Use

    CRISPR

    Tags

    Expression

    Mutation

    WT with tNGFR sequence

    Promoter

    Available since

    July 28, 2022

    Availability

    Academic Institutions and Nonprofits only

    

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16. TFORF0780

    Plasmid

    #141697

    Purpose

    Lentiviral vector for overexpressing transcription factor ORFs with unique 24-bp barcodes. Barcodes facilitate identification of transcription factors in pooled screens.

    Depositor

    Feng Zhang

    Article

    Joung et al Cell. 2023 Jan 5;186(1):209-229.e26. doi

    Insert

    GTF2H1 (GTF2H1 Human)
    

    Use

    Lentiviral

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    EF1a

    Available since

    Jan. 5, 2023

    Availability

    Academic Institutions and Nonprofits only

    

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17. pTR-338 pUC19-tNGFR-P2A-LAG3 N

    Plasmid

    #186055

    Purpose

    Knockin of truncated NGFR to target gene

    Depositor

    Alexander Marson

    Article

    Shy et al Nat Biotechnol. 2022 Aug 25:10.1038/s415

    Insert

    LAG3 (LAG3 Human)
    

    Use

    CRISPR

    Tags

    Expression

    Mutation

    WT with tNGFR sequence

    Promoter

    Available since

    July 28, 2022

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

18. pInducer-TAZ-S89A/S311A/S51A

    Plasmid

    #213590

    Purpose

    Doxycycline inducible expression of TAZ-S89A/S311A/S51A cDNA in mammalian cells

    Depositor

    Channing Der

    Article

    Edwards et al Cancer Res. 2023 Nov 7. doi: 10.1158/000

    Insert

    TAZ (WWTR1 Human)
    

    Use

    Lentiviral

    Tags

    2xHA

    Expression

    Mammalian

    Mutation

    S89A, S311A, S51A

    Promoter

    Available since

    March 11, 2024

    Availability

    Academic Institutions and Nonprofits only

    

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19. Lenti-sgRNA-Cre-GpNLuc-sgmcGAS

    Plasmid

    #208385

    Purpose

    Lentiviral vector expressing Cas9, GpNLuc, and sgRNA targeting murine cGAS gene.

    Depositor

    Gaorav Gupta

    Article

    Cho et al Nature. 2024 Jan 10. doi: 10.1038/s41586

    Insert

    sgcGAS (Cgas Mouse)
    

    Use

    Lentiviral and Luciferase

    Tags

    Expression

    Mammalian

    Mutation

    WT

    Promoter

    Available since

    Jan. 11, 2024

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

20. MSCV-BRD9_BRD7BD-PGK-Puro-IRES-GFP

    Plasmid

    #75118

    Purpose

    Retroviral expression plasmid encoding a human BRD9 bromodomain-swap mutant containing the BRD7 bromodomain

    Depositor

    Christopher Vakoc

    Article

    Hohmann et al Nat Chem Biol. 2016 Jul 4. doi: 10.1038/

    Insert

    BRD9 (BRD9 Human)
    

    Use

    Retroviral

    Tags

    Expression

    Mutation

    Bromodomain replaced with the bromodomain of BRD7

    Promoter

    Available since

    May 18, 2016

    Availability

    Academic Institutions and Nonprofits only

21. pmiRFP-beta-Actin-C-19

    Plasmid

    #108412

    Purpose

    Beta-actin fused to monomeric near-infrared fluorescent protein miRFP

    Depositor

    Edward Boyden

    Article

    Piatkevich et al Nat Chem Biol. 2018 Feb 26. pii: 10.1038

    Insert

    beta-Actin
    

    Use

    Tags

    miRFP

    Expression

    Mammalian

    Mutation

    Promoter

    CMV

    Available since

    April 10, 2018

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

22. pSN25

    Plasmid

    #100110

    Purpose

    expresses a chimeric SOX2-repressor fusion

    Depositor

    Mark Ptashne

    Article

    Narayan et al Cell Rep. 2017 Aug 15;20(7):1585-1596. d

    Insert

    mCitrine-P2A-SOX2 fused to full length HP1 alpha (SOX2, CBX5 Human)
    

    Use

    Lentiviral

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    Available since

    Sept. 12, 2017

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

23. pTR-374 tNGFR-P2A-B2M

    Plasmid

    #186068

    Purpose

    Knockin of truncated NGFR to target gene

    Depositor

    Alexander Marson

    Article

    Shy et al Nat Biotechnol. 2022 Aug 25:10.1038/s415

    Insert

    B2M (B2M Human)
    

    Use

    CRISPR

    Tags

    Expression

    Mutation

    WT with tNGFR sequence

    Promoter

    Available since

    July 28, 2022

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

24. pSF143-wArchon2-KGC-EGFP-ER2

    Plasmid

    #108430

    Purpose

    Codon-optimized Archon2 fluorescent voltage reporter in C.elegans expression plasmid

    Depositor

    Edward Boyden

    Article

    Piatkevich et al Nat Chem Biol. 2018 Feb 26. pii: 10.1038

    Insert

    wArchon2-KGC-EGFP-ER2
    

    Use

    Tags

    Expression

    Worm

    Mutation

    Promoter

    flp-18

    Available since

    April 10, 2018

    Availability

    Academic Institutions and Nonprofits only

    

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25. MSCV-BRD9_BET-PGK-Puro-IRES-GFP

    Plasmid

    #75120

    Purpose

    Retroviral expression plasmid encoding a human BRD9 bromodomain-swap mutant containing the first bromodomain of BRD4

    Depositor

    Christopher Vakoc

    Article

    Hohmann et al Nat Chem Biol. 2016 Jul 4. doi: 10.1038/

    Insert

    BRD9 (BRD9 Human)
    

    Use

    Retroviral

    Tags

    Expression

    Mutation

    Bromodomain replaced with the first bromodomain o…

    Promoter

    Available since

    May 18, 2016

    Availability

    Academic Institutions and Nonprofits only

26. MSCV-BRD9_N216A-PGK-Puro-IRES-GFP

    Plasmid

    #75116

    Purpose

    Retroviral expression plasmid encoding a human BRD9 mutant carrying the N216A single amino acid mutation

    Depositor

    Christopher Vakoc

    Article

    Hohmann et al Nat Chem Biol. 2016 Jul 4. doi: 10.1038/

    Insert

    BRD9 (BRD9 Human)
    

    Use

    Retroviral

    Tags

    Expression

    Mutation

    single amino acid mutation N216A

    Promoter

    Available since

    May 18, 2016

    Availability

    Academic Institutions and Nonprofits only

27. pQCXIP GFP-MOSPD2 W201E

    Plasmid

    #186471

    Purpose

    Expression of human MOSPD2 (mutant W201E, unable to bind lipid droplets) fused to EGFP in mammalian cells

    Depositor

    Catherine Tomasetto

    Article

    Zouiouich et al J Cell Biol. 2022 Jun 6;221(6). pii: 213

    Insert

    MOSPD2 motile sperm domain containing 2 (MOSPD2 Human)
    

    Use

    Retroviral

    Tags

    EGFP

    Expression

    Mammalian

    Mutation

    W201E (no more binding to lipid droplets)

    Promoter

    Available since

    July 13, 2022

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

28. pv6_MECP2_MBD-R106W

    Plasmid

    #179408

    Purpose

    Cell line generation via recombination-mediated cassette exchange (RMCE) and stable expression of MECP2_MBD(R106W)

    Depositor

    Tuncay Baubec

    Article

    Villasenor et al Nat Biotechnol. 2020 Mar 2. pii: 10.1038

    Insert

    MECP2_MBD(R106W) (Mecp2 Mouse)
    

    Use

    Tags

    AviTag and EGFP

    Expression

    Mammalian

    Mutation

    MBD R106W

    Promoter

    CAGGS

    Available since

    Aug. 29, 2022

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

29. pIG-746pUC19-tNGFR-P2A-CD5

    Plasmid

    #186121

    Purpose

    Knockin of truncated NGFR to target gene

    Depositor

    Alexander Marson

    Article

    Shy et al Nat Biotechnol. 2022 Aug 25:10.1038/s415

    Insert

    CD5 (CD5 Human)
    

    Use

    CRISPR

    Tags

    Expression

    Mutation

    WT with tNGFR sequence

    Promoter

    Available since

    July 28, 2022

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

30. pRetroX GFP Mps1 DK

    Plasmid

    #63703

    Purpose

    Inducible expression of GFP-Mps1 DK fusion in mammalian cells

    Depositor

    Floris Foijer

    Article

    Foijer et al Proc Natl Acad Sci U S A. 2014 Sep 16;11

    Insert

    Mps1 (TTK Human)
    

    Use

    Retroviral

    Tags

    EGFP

    Expression

    Mammalian

    Mutation

    deletion of aa 47-156, kinetochore binding domain

    Promoter

    tight TRE promoter

    Available since

    April 22, 2015

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge