We narrowed to 19,235 results for: Tat

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pHAGE-GNAS

Plasmid

#116748

Purpose

Lentiviral expression of GNAS

Depositor

Gordon Mills, Kenneth Scott

Article

Ng et al Cancer Cell. 2018 Mar 12;33(3):450-462.e

Insert

GNAS (GNAS Human)

Use

Lentiviral

Mutation

WT

Available Since

March 14, 2019

Availability

Academic Institutions and Nonprofits only

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pSH-CAG-AtAFB2.F74A-IRES-puro

Plasmid

#216245

Purpose

Express AtAFB2.F74A mutant without tag for AID2

Depositor

Elina Ikonen

Article

Li et al Genome Biol. 2024 Feb 26;25(1):58. doi:

Insert

AtAFB2.F74A

Use

CRISPR and TALEN; Human safe harbor locus (a avs1…

Expression

Mammalian

Mutation

F74A mutation

Promoter

CAG

Available Since

March 27, 2024

Availability

Academic Institutions and Nonprofits only

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LifeAct-14 (LA-14)

Plasmid

#158750

Purpose

Lentiviral plasmid for expression of shorter LifeAct variant (up till 14 amino acids) fused with carboxy terminus eGFP in mammalian cells.

Depositor

Minhajuddin Sirajuddin

Article

Kumari et al EMBO J. 2020 Jun 22:e104006. doi: 10.152

Insert

LifeAct-14

Use

Lentiviral

Tags

eGFP

Expression

Mammalian

Mutation

Deletion mutation for last three amino acids from…

Promoter

CMV immediate early

Available Since

Sept. 9, 2020

Availability

Academic Institutions and Nonprofits only

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pAAV-hSyn-DIO {hCAR}off-{DTR-GFP}on-W3SL

Plasmid

#111396

Purpose

AAV vector with hSynapsin promoter, Cre-OFF hCAR (for efficient CAV-2 infection), Cre-ON DTR-EGFP (diphtheria toxin receptor for cell ablation), and W3SL cassette (for maximize cloning capacity)

Depositor

Adam Kepecs

Article

Li et al Neuron. 2018 Jun 6;98(5):905-917.e5. doi

Inserts

hCAR (CXADR Human)
DTR-EGFP

Use

AAV

Tags

EGFP and Myc

Expression

Mammalian

Promoter

hSynapsin

Available Since

June 7, 2018

Availability

Academic Institutions and Nonprofits only

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pFA6a-kanMX6-PGAL1-3HA

Plasmid

#41608

Depositor

John Pringle

Article

Longtine et al Yeast. 1998 Jul;14(10):953-61.

Insert

GAL1 promoter (GAL1 Budding Yeast)

Use

Yeast genomic targeting

Tags

3xHA, A. gossypii translation elongation factor 1…

Available Since

Dec. 10, 2012

Availability

Academic Institutions and Nonprofits only

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pCDH-mApple

Plasmid

#179383

Purpose

Lentiviral vector for expression of mApple

Depositor

Angus Johnston

Article

Rennick et al Nat Commun. 2022 Oct 12;13(1):6023. doi:

Insert

mApple

Use

Lentiviral

Expression

Mammalian

Promoter

EF1

Available Since

Oct. 11, 2022

Availability

Academic Institutions and Nonprofits only

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pLG031 (upx)

Plasmid

#157909

Purpose

Expresses the upx defense system

Depositor

Feng Zhang

Article

Gao et al Science. 2020 Aug 28;369(6507):1077-1084

Insert

upx (putative PD-(D/E)xK family phosphodiesterase)

Expression

Bacterial

Available Since

Dec. 7, 2020

Availability

Academic Institutions and Nonprofits only

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pRSET-QNA

Plasmid

#129305

Purpose

Negative control for ATP measurement

Depositor

Hiroyuki Noji

Article

Yaginuma et al Sci Rep. 2014 Oct 6;4:6522. doi: 10.1038

Insert

QUEEN-NA

Expression

Bacterial

Available Since

Feb. 27, 2020

Availability

Academic Institutions and Nonprofits only

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pBAD-K-GECO1

Plasmid

#105864

Purpose

Bacterial expression plasmid for red fluorescent Ca2+ indicator K-GECO1

Depositor

Robert Campbell

Article

Shen et al BMC Biol. 2018 Jan 16;16(1):9. doi: 10.1

Insert

K-GECO1

Tags

6-His Tag

Expression

Bacterial

Available Since

April 27, 2018

Availability

Academic Institutions and Nonprofits only

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pCAGGS SARS-CoV-2 BQ.1.1 Spike

Plasmid

#193710

Purpose

Expresses codon-optimized full length SARS-CoV-2 BQ.1.1 Spike

Depositor

Marceline Côté

Article

Tauzin et al Cell Rep. 2023 Jan 31;42(1):111998. doi:

Insert

SARS-CoV-2 BQ.1.1 Spike (S )

Expression

Mammalian

Mutation

Contains the following mutations: T19I, LPPA24S, …

Promoter

chicken β-actin promoter, CMV enhancer

Available Since

Nov. 21, 2022

Availability

Industry, Academic Institutions, and Nonprofits

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pAAV9-null

Plasmid

#200182

Purpose

Expresses AAV2 Rep and AAV9 Cap with nonsense mutation at VR8 site

Depositor

Aravind Asokan

Article

Gonzalez et al Nat Protoc. 2023 Sep 21. doi: 10.1038/s4

Insert

AAV9 Cap w/ nonsense mutation at VR8

Use

AAV

Expression

Mammalian

Mutation

Replaced amino acids 586-592 in Cap with nonsense…

Available Since

Oct. 31, 2023

Availability

Academic Institutions and Nonprofits only

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pet-MBP

Plasmid

#209184

Purpose

Expression of recombinant proteins with N-terminal MBP-tag and C-terminal Hisx6-tag in E. coli

Depositor

Attila Reményi

Article

Poti et al Int J Mol Sci. 2023 Oct 3;24(19):14854.

Type

Empty backbone

Tags

HisX6

Expression

Bacterial

Promoter

T7

Available Since

April 2, 2024

Availability

Academic Institutions and Nonprofits only

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AAV-Ef1a-InteinC-CreC

Plasmid

#187615

Purpose

AAV vector encoding C-terminal portion of split-Intein-mediated split-Cre

Depositor

Il Hwan Kim

Article

Kim et al Cell Rep. 2022 Jun 7;39(10):110906. doi:

Insert

InteinC-CreC

Use

AAV

Expression

Mammalian

Available Since

July 21, 2022

Availability

Academic Institutions and Nonprofits only

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plentiCRISPRv2-sgEGFP

Plasmid

#86153

Purpose

Lentivirus carrying Cas9/CRISPR for cut in GFP (used as control)

Depositor

Roland Friedel

Article

Huang et al Commun Biol. 2021 Jan 29;4(1):145. doi:

Insert

EGFP

Use

CRISPR and Lentiviral

Available Since

Jan. 13, 2021

Availability

Academic Institutions and Nonprofits only

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pCLASP_mScarlet_CLASP_RGS2membrane

Plasmid

#133086

Purpose

Plasmid contains mScarlet with CLASP. This consists of the plasma membrane anchor (pm-LOVTRAP) and the cargo (Zdk-mScarlet-yeLANS). CLASP modulates nuclear localization of mScarlet in response to bl

Depositor

Hana El-Samad

Article

Chen et al Cell Syst. 2020 Sep 4. pii: S2405-4712(2

Insert

mScarlet-CLASP

Expression

Bacterial and Yeast

Available Since

Sept. 16, 2020

Availability

Academic Institutions and Nonprofits only

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pXPR003-sgTP53-3

Plasmid

#118021

Purpose

Constitutive expression of sgRNA targeting human TP53

Depositor

William Hahn, David Root

Article

Giacomelli et al Nat Genet. 2018 Oct;50(10):1381-1387. do

Insert

sgTP53-3 (TP53 Human)

Use

Lentiviral

Expression

Mammalian

Promoter

hU6

Available Since

Oct. 29, 2018

Availability

Academic Institutions and Nonprofits only

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pCORE-Hp53

Plasmid

#72242

Purpose

Contains the CORE cassette with hyg and human p53 gene markers

Depositor

Michael Resnick, Francesca Storici

Article

Storici et al Genet Eng (N Y). 2003;25:189-207.

Inserts

p53 (TP53 Human)
hyg

Expression

Bacterial

Mutation

V122A mutation

Available Since

Jan. 29, 2016

Availability

Academic Institutions and Nonprofits only

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pSicoR-Ef1a-mCh-GFPi

Plasmid

#31849

Depositor

Bruce Conklin

Article

Salomonis et al Proc Natl Acad Sci U S A. 2010 Jun 8;107

Insert

GFP RNAi

Use

Cre/Lox, Lentiviral, and RNAi

Expression

Mammalian

Promoter

U6 for shRNA; Ef1alpha for mCherry

Available Since

Jan. 3, 2012

Availability

Academic Institutions and Nonprofits only

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pCAD64_pCMV6_Entry_hIrg1

Plasmid

#124879

Purpose

Expresses hCAD His103Ala mutant in mammalian cells

Depositor

Konrad Buessow

Article

Chen et al Proc Natl Acad Sci U S A. 2019 Sep 23. p

Insert

cis-aconitate decarboxylase (ACOD1 Human)

Tags

myc and FLAG tags

Expression

Mammalian

Mutation

mutated His103 to Ala

Promoter

CMV

Available Since

Sept. 16, 2019

Availability

Academic Institutions and Nonprofits only

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pGL3-Gateway-DSCP

Plasmid

#71506

Purpose

flySTARR-seq validation vector with Gateway cassette upstream of the firefly luciferase gene and the DSCP core promoter

Depositor

Alexander Stark

Article

Arnold et al Science. 2013 Mar 1;339(6123):1074-7. do

Type

Empty backbone

Use

Flystarr-seq validation vector

Tags

luciferase

Expression

Insect

Available Since

Dec. 29, 2015

Availability

Academic Institutions and Nonprofits only

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