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Enter a sequence to perform a BLAST-based search of Addgene plasmid sequence databases.

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We narrowed to 11,938 results for: emb

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Showing: 3181 - 3200 of 11938 results

  2. pelB-MBP-BB0238-ss

    Plasmid
    #137046
    Purpose
    E. coli expression clone (T7lac promoter) for mature BB0238 (aa 21-256) from B. burgdorferi with N-terminal PelB signal peptide + His10 + mature maltose binding protein + TEV protease cleavage
    Depositor
    Debra Hansen
    Publication
    Robertson et al Sci Rep. 2019 Nov 26;9(1):17606. doi: 10
    Insert
    AAC66635.2 (BB_0238 Borrelia burgdorferi B31)
    Tags
    PelB signal peptide + His10 + maltose binding pro…
    Expression
    Bacterial
    Mutation
    mature BB0238: lacks the BB0238 signal peptide
    Promoter
    T7lac
    Available since
    April 22, 2020
    Availability
    Academic Institutions and Nonprofits only
    137046-plasmid-map-sequence-id-292071

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  3. pEcRNAP6

    Plasmid
    #128940
    Purpose
    pET21a derivative encoding E. coli core RNA polymerase subunits: RpoA, B, C-ppx(His)10, Z
    Depositor
    Seth Darst
    Publication
    Twist et al Protein Sci. 2011 Jun;20(6):986-95. doi:
    Inserts
    RpoA (rpoA E. coli)
    RpoB
    RpoC-ppx(His)10
    RpoZ
    Tags
    His
    Expression
    Bacterial
    Mutation
    PreScission protease site (GE Healthcare; LEVLFQG…
    Promoter
    T7
    Available since
    Aug. 29, 2019
    Availability
    Academic Institutions and Nonprofits only
    Cited by
    128940-plasmid-map-sequence-id-254597

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  4. mCherry-FKBP-SAC1-HLx8

    Plasmid
    #108136
    Purpose
    Recruitable SAC1 with extended linker
    Depositor
    Gerry Hammond
    Publication
    Zewe et al Elife. 2018 Feb 20;7. pii: 35588. doi: 1
    Has service
    50 µg Transfection-Ready DNA and 100 µg Transfection-Ready DNA
    Insert
    mCherry:FKBP1A(3-108):[GGSA]4GG:SACM1L(1-520):[EAAAR]8:SACM1L(521-587) (SACM1L Human)
    Expression
    Mammalian
    Mutation
    SACM1L(1-520):[EAAAR]8:SACM1L(521-587)
    Promoter
    CMV
    Available since
    April 6, 2018
    Availability
    Academic Institutions and Nonprofits only
    108136-plasmid-map-sequence-id-207723

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  6. pGL-p21UTRm1

    Plasmid
    #20878
    Depositor
    Robert Blelloch
    Publication
    Wang et al Nat Genet. 2008 Dec . 40(12):1478-83.
    Has service
    50 µg Transfection-Ready DNA and 100 µg Transfection-Ready DNA
    Insert
    p21 3'UTR site 1 mutation (Cdkn1a Mouse)
    Use
    Luciferase
    Expression
    Mammalian
    Mutation
    Predicted miRNA binding site 1 (Position ~420) GC…
    Available since
    Aug. 5, 2009
    Availability
    Academic Institutions and Nonprofits only

  8. pPB-tetO(hCMV1)-HA-Tet1mHxD(201R2)-IV

    Plasmid
    #102422
    Purpose
    Inducible expression of siRNA resistant mouse Tet1-201 (ENSMUST00000050826.13) with mutated catalytic domain (H1620Y & D1622A), HA-tag and IRES-Venus in piggyBac (PB) transposon vector
    Depositor
    Kian Peng Koh
    Publication
    Khoueiry et al Nat Genet. 2017 Jul;49(7):1061-1072. doi
    Insert
    Tet1 catalytic domain mutant (Tet1 Mouse)
    Tags
    HA
    Expression
    Mammalian
    Mutation
    H1620Y and D1622A mutations in Tet1 catalytic dom…
    Promoter
    TetO-CMV
    Available since
    Nov. 20, 2017
    Availability
    Academic Institutions and Nonprofits only
    102422-plasmid-map-sequence-id-198789

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  9. mCherry-CRY2-P14P5K

    Plasmid
    #79570
    Purpose
    expression of mCherry-tagged CRY2PHR fused to iSH2 domain of mouse Pip5k1c
    Depositor
    Olof Idevall-Hagren
    Publication
    Xie et al Cell Chem Biol. 2016 Jul 21;23(7):816-26
    Has service
    50 µg Transfection-Ready DNA and 100 µg Transfection-Ready DNA
    Insert
    Pip5k1c (Pip5k1c Mouse)
    Tags
    CRY2 (photolyase homology region domain of Arabid…
    Expression
    Mammalian
    Mutation
    R445E and R446E; truncation of amino acids 636-66…
    Promoter
    CMV
    Available since
    Aug. 9, 2016
    Availability
    Academic Institutions and Nonprofits only

  14. InterCatch-3xL

    Plasmid
    #186909
    Purpose
    Sleeping Beauty transposon system with constitutive bi-directional promoter, one side expressing SpyCatcher003-sfCherry1-10 fused to TfR through (GGGGS)3 linker and the other side expressing EGFP and puromycin resistance gene
    Depositor
    Taekjip Ha, Allen Liu
    Publication
    Moghimianavval et al Small. 2022 May 26:e2202104. doi: 10.100
    Insert
    TfR-(GGGGS)3-SpyCatcher003-sfCherry1-10
    Expression
    Mammalian
    Available since
    Dec. 6, 2022
    Availability
    Academic Institutions and Nonprofits only
    186909-plasmid-map-sequence-id-372903

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  19. NST1_pOpOn2.1

    Plasmid
    #228065
    Purpose
    Expresses NST1 in different cell types in Arabidopsis thaliana upon induction with dexamethasone.
    Depositor
    Charles Anderson
    Publication
    Tamadaddi et al Ann Bot. 2024 Aug 30:mcae153. doi: 10.10
    Insert
    NAC SECONDARY WALL THICKENING PROMOTING FACTOR1, NST1 (NST1 Mustard Weed)
    Use
    Dexamethasone inducible vector
    Expression
    Plant
    Promoter
    CaMV35S for LhGR and pOp6-minimal CaMV35S for NST1
    Available since
    Jan. 2, 2025
    Availability
    Academic Institutions and Nonprofits only
    228065-plasmid-map-sequence-id-456794

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Showing: 3181 - 3200 of 11938 results