We narrowed to 5,340 results for: 290

1. pL-CRISPR.EFS.GFP

   Plasmid

   #57818

   Purpose

   Lentiviral CRISPR-Cas9 delivery for SpCas9 and sgRNA. Coexpresses eGFP via P2A cleavage site. EFS Promoter driven

   Depositor

   Benjamin Ebert

   Article

   Heckl et al Nat Biotechnol. 2014 Jun 22. doi: 10.103

   Inserts

   SpCas9
   Sp sgRNA scaffold
   EFS (EEF1A1 Human)
   P2A-eGFP
   

   Use

   CRISPR and Lentiviral

   Tags

   FLAG

   Promoter

   EFS and hU6

   Available Since

   Aug. 20, 2014

   Availability

   Academic Institutions and Nonprofits only

   

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2. pT7NT*-Bri2 113-231 R221E

   Plasmid

   #138134

   Purpose

   Expresseds human Bri2 BRICHOS R221E mutant in E. coli

   Depositor

   Jan Johansson

   Article

   Chen et al Commun Biol. 2020 Jan 20;3(1):32. doi: 1

   Insert

   human Bri2 BRICHOS R221E (ITM2B Human)
   

   Tags

   NT* tag derived from spider silk proteins

   Expression

   Bacterial

   Promoter

   T7

   Available Since

   May 28, 2020

   Availability

   Academic Institutions and Nonprofits only

   

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3. pF1-Hs Cdk1/cyclin B1

   Plasmid

   #177330

   Purpose

   Co-expression of the human active Cdk1/cyclin B1 kinase complex

   Depositor

   Andreas Boland

   Article

   Yu et al Nature. 2021 Aug;596(7870):138-142. doi:

   Inserts

   Cyclin-dependent kinase 1 (CDK1 Human)
   G2/mitotic-specific cyclin-B1 (CCNB1 Human)
   

   Tags

   2 x Strep-tag and 8 x His

   Expression

   Insect

   Available Since

   Nov. 19, 2021

   Availability

   Academic Institutions and Nonprofits only

   

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4. pX458-Ef1a-Cas9-H2B-mCherry (dual gRNA: Neurog2 x2)

   Plasmid

   #171100

   Purpose

   CRISPR/Cas9 expressing plasmid containing two gRNAs targeting mouse Neurog2.

   Depositor

   Joseph Brzezinski

   Article

   Kaufman et al Development. 2021 Jun 15;148(12). pii: 2

   Insert

   hSpCas9 (Neurog2 S. pyogenes, Mouse)
   

   Use

   CRISPR

   Promoter

   Ef1a

   Available Since

   June 28, 2021

   Availability

   Academic Institutions and Nonprofits only

   

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5. Lenti-sgPten#2/Cre

   Plasmid

   #173646

   Purpose

   Expresses a Pten-targeting gRNA and Cre-recombinase

   Depositor

   Charles Swanton

   Article

   Cai et al Cancer Discov. 2021 Feb 19. pii: 2159-82

   Insert

   gRNA targeting Pten (Pten Mouse)
   

   Use

   CRISPR, Cre/Lox, and Lentiviral

   Available Since

   June 7, 2022

   Availability

   Academic Institutions and Nonprofits only

   

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6. Lenti-sgTp53#2/Cre

   Plasmid

   #173621

   Purpose

   Expresses a Tp53-targeting gRNA and Cre-recombinase

   Depositor

   Charles Swanton

   Article

   Cai et al Cancer Discov. 2021 Feb 19. pii: 2159-82

   Insert

   gRNA targeting Tp53 (Trp53 Mouse)
   

   Use

   CRISPR, Cre/Lox, and Lentiviral

   Available Since

   June 7, 2022

   Availability

   Academic Institutions and Nonprofits only

   

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7. Lenti-sgTp53#3/Cre

   Plasmid

   #173622

   Purpose

   Expresses a Tp53-targeting gRNA and Cre-recombinase

   Depositor

   Charles Swanton

   Article

   Cai et al Cancer Discov. 2021 Feb 19. pii: 2159-82

   Insert

   gRNA targeting Tp53 (Trp53 Mouse)
   

   Use

   CRISPR, Cre/Lox, and Lentiviral

   Available Since

   June 7, 2022

   Availability

   Academic Institutions and Nonprofits only

   

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8. Lenti-sgPten#1/Cre

   Plasmid

   #173645

   Purpose

   Expresses a Pten-targeting gRNA and Cre-recombinase

   Depositor

   Charles Swanton

   Article

   Cai et al Cancer Discov. 2021 Feb 19. pii: 2159-82

   Insert

   gRNA targeting Pten (Pten Mouse)
   

   Use

   CRISPR, Cre/Lox, and Lentiviral

   Available Since

   May 18, 2022

   Availability

   Academic Institutions and Nonprofits only

   

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9. PB-tetO-NB

   Plasmid

   #197092

   Purpose

   PiggyBac plasmid with tet-inducible expression of transcription factors for sensory neuron differentiation

   Depositor

   Brian Wainger

   Article

   Held et al Cell Rep. 2023 Aug 30;42(9):113046. doi:

   Insert

   NGN2-T2A-BRN3A (NEUROG2, POU4F1 Human)
   

   Expression

   Mammalian

   Promoter

   TRE3G

   Available Since

   Nov. 27, 2023

   Availability

   Academic Institutions and Nonprofits only

   

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10. pmiRA1-MYB

    Plasmid

    #141120

    Purpose

    Lentiviral overexpression of MYB in mammalian cells.

    Depositor

    Jianjun Chen

    Article

    Weng et al Cell Stem Cell. 2018 Feb 1;22(2):191-205

    Insert

    MYB (MYB Human)
    

    Use

    Lentiviral

    Expression

    Mammalian

    Available Since

    Dec. 14, 2020

    Availability

    Academic Institutions and Nonprofits only

    

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11. pDONR221_MFSD14A

    Plasmid

    #131869

    Purpose

    Gateway entry clone with codon-optimized ORF sequence for subcloning into custom expression vectors

    Depositor

    Giulio Superti-Furga, RESOLUTE Consortium

    Article

    Superti-Furga et al Nat Rev Drug Discov. 2020 Jul;19(7):429-

    Insert

    MFSD14A (HIAT1 Human)
    

    Expression

    Mammalian

    Available Since

    Oct. 9, 2019

    Availability

    Industry, Academic Institutions, and Nonprofits

    

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12. pDONR221-MFSD14A_STOP

    Plasmid

    #161035

    Purpose

    Gateway entry clone with codon-optimized ORF sequence for subcloning into custom expression vectors. Contains a STOP codon at the end of the codon-optimized ORF sequence.

    Depositor

    Giulio Superti-Furga, RESOLUTE Consortium

    Article

    Superti-Furga et al Nat Rev Drug Discov. 2020 Jul;19(7):429-

    Insert

    MFSD14A (HIAT1 Human)
    

    Expression

    Mammalian

    Available Since

    June 21, 2021

    Availability

    Industry, Academic Institutions, and Nonprofits

    

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13. pC0054-CMV-dPspCas13b-longlinker-ADAR2DD(E488Q/T375G)

    Plasmid

    #103870

    Purpose

    High specificity version of dPspCas13b-ADAR2DD(E488Q) fusions. T375G mutation in the ADAR deaminase domain confers increased specificity with slightly reduced activity.

    Depositor

    Feng Zhang

    Article

    Cox et al Science. 2017 Oct 25. pii: eaaq0180. doi

    Inserts

    PspCas13b
    huADAR2DD (ADARB1 Human)
    

    Use

    CRISPR

    Tags

    GSGGGGS and HIV NES

    Expression

    Mammalian

    Mutation

    Changed histidne 133 to alanine and histidine 105…

    Available Since

    Dec. 13, 2017

    Availability

    Academic Institutions and Nonprofits only

    

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14. pFastBac Dual wt p110α/p85α

    Plasmid

    #199302

    Purpose

    co-expression of His-tagged p110α wt and Avi-tagged p85α wt proteins

    Depositor

    Peter Vogt

    Article

    Favalli et al Nat Chem. 2021 Jul;13(7):714. doi: 10.10

    Insert

    His-tagged p110α wt and Avi-tagged p85α wt (PIK3CA, PIK3R1 Human)
    

    Tags

    Avi and His

    Expression

    Insect

    Available Since

    June 1, 2023

    Availability

    Academic Institutions and Nonprofits only

    

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15. pT3-EF1aH-PIK3CA H1047R

    Plasmid

    #180031

    Purpose

    This plasmid is in the pT3-EF1a vector without loxP sites flanking the inverted repeats of SB (sleeping beauty) sequence. Therefore this plasmid can be used in LoxP mice.

    Depositor

    Xin Chen

    Article

    Wang et al Liver Int. 2015 Dec 30. doi: 10.1111/liv

    Insert

    PIK3CA H1047R (PIK3CA Human)
    

    Tags

    HA tag

    Expression

    Mammalian

    Mutation

    H1047R

    Promoter

    EF1a

    Available Since

    March 28, 2022

    Availability

    Academic Institutions and Nonprofits only

    

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16. PB-TO-hNIL BSD-mApple

    Plasmid

    #182313

    Purpose

    Piggybac Tet-ON plasmid for differentiating hiPSCs into lower motor neurons via NGN2, ISL1, and LHX3 expression, bsd selection, mApple

    Depositor

    iPSC Neurodegenerative Disease Initiative (iNDI), Michael Ward

    Inserts

    hNGN2 (NEUROG2 Human)
    hISL1 (ISL1 Human)
    hLHX3 (LHX3 Human)
    bsd-T2A-mycNLS-mApple
    

    Tags

    T2A-mycNLS-mApple

    Expression

    Mammalian

    Available Since

    April 14, 2022

    Availability

    Academic Institutions and Nonprofits only

    

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17. pLEX-EF1a ANXA11-mEmerald

    Plasmid

    #164210

    Purpose

    pLEX lentivirus backbone expresses mEmerald tagged ANXA11 under EF1a promoter

    Depositor

    Jennifer Lippincott-Schwartz

    Article

    Liao et al Cell. 2019 Sep 19;179(1):147-164.e20. do

    Insert

    ANXA11 (ANXA11 Human)
    

    Use

    Lentiviral

    Tags

    mEmerald

    Promoter

    EF1a

    Available Since

    Jan. 25, 2021

    Availability

    Academic Institutions and Nonprofits only

    

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18. pLL3.7 FLAG-YAP1-TEAD-P-H2B-mCherry

    Plasmid

    #128327

    Purpose

    Reporter to evaluate YAP1/TEAD-mediated gene transcription

    Depositor

    Yutaka Hata

    Article

    Maruyama et al Mol Cancer Res. 2018 Feb;16(2):197-211.

    Inserts

    H2B mCherry
    YAP1 (YAP1 Human)
    

    Use

    Lentiviral

    Tags

    FLAG

    Expression

    Mammalian

    Available Since

    March 18, 2020

    Availability

    Academic Institutions and Nonprofits only

    

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19. P932_CLYBL-CBX3-Cbh-Zeo-TetO-NEUROG2

    Plasmid

    #202762

    Purpose

    Encodes one forebrain neuron specific transcription factor under the control of a TetOn promoter at the CLYBL safe harbor

    Depositor

    Karl Wahlin

    Article

    Agarwal et al NPJ Regen Med. 2023 Sep 29;8(1):55. doi:

    Insert

    NEUROG2 (NEUROG2 Human)
    

    Expression

    Mammalian

    Promoter

    TetO

    Available Since

    Feb. 26, 2024

    Availability

    Academic Institutions and Nonprofits only

    

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20. pRSF-MCM3/5

    Plasmid

    #116950

    Purpose

    Expresses human MCM3 and human MCM5

    Depositor

    James Chong

    Article

    Hesketh et al J Biol Chem. 2015 Mar 20;290(12):7973-9.

    Inserts

    Human MCM5 (MCM5 Human)
    human MCM3 (MCM3 Human)
    

    Tags

    His6

    Expression

    Bacterial

    Available Since

    Nov. 12, 2018

    Availability

    Academic Institutions and Nonprofits only

    

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