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Plasmid#130661PurposepCMV-dCas13b6-mapkNES-GS-dADAR2(E488Q/V351G/S486A/T375S/S370C/P462A/N597I/L332I/I398V/K350I/M383L/D619G/S582T/V440I/S495N/K418E/DepositorInsertdRanCas13b-ADAR2dd (RESCUE)
UseTagsExpressionMammalianMutationPromoterAvailable sinceOct. 21, 2019AvailabilityAcademic Institutions and Nonprofits only -
pcDNA3.1-hSTING (V155M)
Plasmid#214161PurposeTransient expression of gain-of-function STING mutantDepositorInsertSTING (V155M) (STING1 Human)
UseTagsExpressionMammalianMutationV155MPromoterCMVAvailable sinceMarch 5, 2024AvailabilityAcademic Institutions and Nonprofits only -
pMSP1E3D1
Plasmid#20066PurposeBacterial expression of an extended membrane scaffold protein (MSP1E3D1) for Nanodisc formationDepositorInsertMSP1E3D1
UseTags7-HisExpressionBacterialMutation"extended" MSP1D1; contains repeats of …PromoterAvailable sinceJan. 12, 2009AvailabilityAcademic Institutions and Nonprofits only -
pLifeAct-HyPer7
Plasmid#136464PurposeMammalian expression of F-actin targeted ultrasensitive hydrogen peroxide indicator HyPer7 fused with LifeAct peptide for optical imagingDepositorInsertHyPer7
UseTagsLifeActExpressionMammalianMutationPromoterCMVAvailable sinceFeb. 11, 2020AvailabilityAcademic Institutions and Nonprofits only -
pGEX4T1-human beta-catenin full length
Plasmid#132609Purposeexpresses human beta-catenin full length in E. coliDepositorInsertbeta-catenin
UseTagsExpressionBacterialMutationPromoterAvailable sinceOct. 17, 2019AvailabilityAcademic Institutions and Nonprofits only -
pSpCas9_T2A_mCherry sg1-sg2
Plasmid#192479PurposeAll-in-one plasmid containing both guides for cutting BFP region in DSB Spectrum V1DepositorInsertSpCas9
UseTagsT2A mCherryExpressionMammalianMutationPromoterAvailable sinceDec. 7, 2022AvailabilityAcademic Institutions and Nonprofits only -
pKSJ340
Plasmid#27125DepositorInsertColicin Ia + Ia immunity protein
UseTagsExpressionBacterialMutationPromoterAvailable sinceJan. 20, 2011AvailabilityAcademic Institutions and Nonprofits only -
TFORF0547
Plasmid#142645PurposeLentiviral vector for overexpressing transcription factor ORFs with unique 24-bp barcodes. Barcodes facilitate identification of transcription factors in pooled screens.DepositorInsertPAX9 (PAX9 Human)
UseLentiviralTagsExpressionMammalianMutationPromoterEF1aAvailable sinceApril 27, 2023AvailabilityAcademic Institutions and Nonprofits only -
MG1655 ΔendA ΔrecA (DE3)
Bacterial Strain#37854DepositorBacterial ResistanceNoneAvailable sinceNov. 6, 2012AvailabilityAcademic Institutions and Nonprofits only -
FUGW-PercevalHR
Plasmid#49083PurposeLentiviral vector for PercevalHR, a fluorescent sensor of ATP-to-ADP ratio.DepositorInsertPercevalHR
UseLentiviralTagsExpressionMammalianMutationPromoterAvailable sinceNov. 7, 2013AvailabilityAcademic Institutions and Nonprofits only -
pCMV-FLAG-CAMK2D
Plasmid#221687PurposeMammalian expression of FLAG-tagged human CAMK2DDepositorInsertCAMK2D (CAMK2D Human)
UseTagsExpressionMammalianMutationPromoterAvailable sinceJuly 29, 2024AvailabilityAcademic Institutions and Nonprofits only -
pSpCas9_T2A_iRFP670 sgBFP
Plasmid#192478PurposeAll-in-one plasmid for cutting BFP region in DSB Spectrum V2 or V3DepositorInsertSpCas9
UseTagsT2A iRFP670ExpressionMammalianMutationPromoterAvailable sinceDec. 7, 2022AvailabilityAcademic Institutions and Nonprofits only -
AAV pCAG-FLEX-EGFP-WPRE (AAV2)
Viral Prep#51502-AAV2PurposeReady-to-use AAV2 particles produced from AAV pCAG-FLEX-EGFP-WPRE (#51502). In addition to the viral particles, you will also receive purified AAV pCAG-FLEX-EGFP-WPRE plasmid DNA. CAG-driven, Cre dependent EGFP expression control. These AAV preparations are suitable purity for injection into animals.DepositorPromoterCAGTagsEGFP (Cre-dependent)Available sinceMarch 7, 2018AvailabilityAcademic Institutions and Nonprofits only -
pETM6-At4CL-PhCHS-CmCHI (C5 mutant, pFlavo-opt)
Plasmid#73404PurposeExpresses three enzymes to convert phenylpropanoic acids to flavanones in vivoDepositorInsertsAt4CL
PhCHS
CmCHI
UseTagsExpressionBacterialMutationPromoterT7Available sinceMarch 1, 2016AvailabilityAcademic Institutions and Nonprofits only -
rAAV2/MG1.2
Plasmid#184541PurposeAAV packaging plasmid, expressing Rep2 and AAV-MG1.2 capsidDepositorInsertAAV-MG1.2 capsid
UseAAV; Helper vector for packagingTagsExpressionMutationchanges on AAV9 capsid: K449R, Q588T; insertion o…PromoterAvailable sinceJuly 22, 2022AvailabilityAcademic Institutions and Nonprofits only -
pAAV-CaMKIIa-HA-hM4D(Gi)-IRES-mCitrine (AAV2)
Viral Prep#50467-AAV2PurposeReady-to-use AAV2 particles produced from pAAV-CaMKIIa-HA-hM4D(Gi)-IRES-mCitrine (#50467). In addition to the viral particles, you will also receive purified pAAV-CaMKIIa-HA-hM4D(Gi)-IRES-mCitrine plasmid DNA. CaMKIIa-driven HA-tagged hM4D(Gi) and bicistronic mCitrine expression, for chemogenetic inhibition. These AAV preparations are suitable purity for injection into animals.DepositorPromoterCaMKIITagsmCitrineAvailable sinceMay 10, 2019AvailabilityAcademic Institutions and Nonprofits only -
pemiRFP670-N1
Plasmid#136556PurposeA cytoplasmic expression of a monomeric phytochrome-based near-infrared fluorescent protein emiRFP670 (enhanced miRFP670)DepositorInsertemiRFP670
UseTagsExpressionMammalianMutationPromoterCMVAvailable sinceFeb. 11, 2020AvailabilityAcademic Institutions and Nonprofits only -
AAV-pgk-Cre (AAV Retrograde)
Viral Prep#24593-AAVrgPurposeReady-to-use AAV Retrograde particles produced from AAV-pgk-Cre (#24593). In addition to the viral particles, you will also receive purified AAV-pgk-Cre plasmid DNA. Cre expression under the PGK promoter. These AAV were produced with a retrograde serotype, which permits retrograde access to projection neurons.DepositorPromoterpgkTagsNoneAvailable sinceJuly 24, 2017AvailabilityAcademic Institutions and Nonprofits only -
pMSCV-dCasRx-ALKBH5-PURO
Plasmid#175582PurposeExpresses dCasRx-ALKBH5 in mammalian cellDepositorInsertdCasRx-ALKBH5
UseCRISPR and LentiviralTagsFLAG-HAExpressionMammalianMutationRfxCas13d R239A/H244A/R858A/H863APromoterpMSCVAvailable sinceNov. 5, 2021AvailabilityAcademic Institutions and Nonprofits only -
pCMV-Neo-Bam p53 R273H
Plasmid#16439DepositorInsertp53 R273H (TP53 Human)
UseTagsExpressionMammalianMutationR273HPromoterAvailable sinceMarch 12, 2008AvailabilityAcademic Institutions and Nonprofits only -
pENN.AAV.CamKII.GCaMP6f.WPRE.SV40 (AAV5)
Viral Prep#100834-AAV5PurposeReady-to-use AAV5 particles produced from pENN.AAV.CamKII.GCaMP6f.WPRE.SV40 (#100834). In addition to the viral particles, you will also receive purified pENN.AAV.CamKII.GCaMP6f.WPRE.SV40 plasmid DNA. CamKII-driven GCaMP6f calcium sensor. These AAV preparations are suitable purity for injection into animals.DepositorPromoterCaMKIITagsNoneAvailable sinceApril 25, 2018AvailabilityAcademic Institutions and Nonprofits only -
PIEZO1-HaloTag
Plasmid#207834PurposePIEZO1 fusion for biochemistry and live imagingDepositorInsertPIEZO1 (PIEZO1 Human)
UseTagsHaloTagExpressionMammalianMutationPromoterCMVAvailable sinceNov. 15, 2023AvailabilityAcademic Institutions and Nonprofits only -
pGWB-cLUC
Plasmid#174051PurposeGateway compatible plasmid containing C-terminal luciferase for split-luciferase complementation assayDepositorTypeEmpty backboneUseLuciferaseTagsExpressionPlantMutationPromoterAvailable sinceSept. 22, 2021AvailabilityAcademic Institutions and Nonprofits only -
pCE-hOCT3/4
Plasmid#41813PurposeNon-integrating (episomal) expression of human OCT3/4DepositorInsertPOU5F1 (POU5F1 Human)
UseTagsExpressionMammalianMutationPromoterCAGAvailable sinceApril 9, 2013AvailabilityAcademic Institutions and Nonprofits only -
pET15b-hnRNP-H
Plasmid#23020DepositorInserthnRNP-H (HNRNPH1 Human)
UseTagsHisExpressionBacterialMutationPromoterAvailable sinceJune 4, 2010AvailabilityAcademic Institutions and Nonprofits only -
plRL117
Plasmid#163635PurposeOptimized M. smegmatis CRISPRi plasmid; L5 integrating.DepositorTypeEmpty backboneUseCRISPRTagsExpressionBacterialMutationPromoterTet-OnAvailable sinceAug. 11, 2021AvailabilityAcademic Institutions and Nonprofits only -
pST-2K
Plasmid#44564Purposefor ectopic constitutive expression of two mycobacterial proteins in one vectorDepositorTypeEmpty backboneUseE.coli-mycobacteria shuttle vectorTagsFlag tag and His tagExpressionBacterialMutationPromoterAvailable sinceFeb. 18, 2014AvailabilityAcademic Institutions and Nonprofits only -
pEGFP-C1-G3BP1-DeltaRBP
Plasmid#135999PurposeG3BP1 with RNA binding domains deleted inserted with GFP tagged on the N-terminusDepositorInsertG3BP1 (G3BP1 Human)
UseTagsEGFPExpressionMammalianMutationC-term of G3BP1 Deleted for Delta RBPPromoterAvailable sinceFeb. 4, 2020AvailabilityAcademic Institutions and Nonprofits only -
pGL3-NFAT luciferase
Plasmid#17870Purpose3x NFAT binding sequence upstream of a luciferase reporter; can be used to assay signal transduction through Ca2+, calcineurin and NFAT in any tissueDepositorInsert3x NFAT binding sequence (IL2 )
UseLuciferaseTagsIL2 promoter and LuciferaseExpressionMammalianMutationPromoterIL-2 promoterAvailable sinceApril 29, 2008AvailabilityAcademic Institutions and Nonprofits only -
pCas9-HE-Geminin
Plasmid#109402PurposeExpresses Cas9 fusion to HE (HDR-enhancer) domain of human CtIP protein and Geminin degronDepositorInsertHE-Geminin
UseTagsExpressionMutationPromoterAvailable sinceJune 15, 2018AvailabilityAcademic Institutions and Nonprofits only