We narrowed to 12,735 results for: icos

1. pSCBE3-NG-HF1-Hypa

   Plasmid

   #218162

   Purpose

   This plasmid harbors the base editor SCBE3-NG-HF1-Hypa along with an sgRNA cloning cassette, facilitating high-fidelity cytosine base editing at targets bearing NG PAM in Streptomyces.

   Depositor

   Yuhui Sun

   Article

   Wang et al Nat Commun. 2024 Jul 7;15(1):5687. doi:

   Inserts

   scbe3-NG-HF1-Hypa
   a programmable sgRNA cloning cassette
   

   Use

   CRISPR; Sgrna transcription

   Tags

   Expression

   Bacterial

   Mutation

   Mutations in the portion of SpCas9 : D10A / N497A…

   Promoter

   rpsL promoter

   Available since

   May 13, 2024

   Availability

   Academic Institutions and Nonprofits only

   

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2. pCMV_msfGFP-SEPT7 Gmut2

   Plasmid

   #180425

   Purpose

   mammalian expression of human SEPT7 Gmut2 fused to monomeric superfolder GFP

   Depositor

   Manos Mavrakis

   Article

   Martins et al J Cell Biol. 2023 Mar 6;222(3):e20220301

   Insert

   SEPTIN7 (SEPTIN7 Human)
   

   Use

   Tags

   msfGFP

   Expression

   Mammalian

   Mutation

   SEPT7 H279D

   Promoter

   CMV

   Available since

   Dec. 1, 2022

   Availability

   Academic Institutions and Nonprofits only

   

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3. pCMV_SEPT9_i3 Gmut-msfGFP

   Plasmid

   #180326

   Purpose

   mammalian expression of human SEPT9_i3 Gmut fused to monomeric superfolder GFP

   Depositor

   Manos Mavrakis

   Article

   Martins et al J Cell Biol. 2023 Mar 6;222(3):e20220301

   Insert

   SEPTIN9_v3 (SEPTIN9 Human)
   

   Use

   Tags

   msfGFP

   Expression

   Mammalian

   Mutation

   SEPT9_i3 W502A, H512D

   Promoter

   CMV

   Available since

   Dec. 1, 2022

   Availability

   Academic Institutions and Nonprofits only

   

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4. pnEA-vH_His-TEV-SEPT2 NCmut-msfGFP

   Plasmid

   #180311

   Purpose

   bacterial expression of human SEPT2 NCmut fused to monomeric superfolder GFP

   Depositor

   Manos Mavrakis

   Article

   Martins et al J Cell Biol. 2023 Mar 6;222(3):e20220301

   Insert

   SEPTIN2 (SEPTIN2 Human)
   

   Use

   Tags

   His6-TEV and msfGFP

   Expression

   Bacterial

   Mutation

   SEPT2 F20D, V27D

   Promoter

   Available since

   Dec. 1, 2022

   Availability

   Academic Institutions and Nonprofits only

   

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5. pCMV_msfGFP-SEPT7 Gmut1

   Plasmid

   #180316

   Purpose

   mammalian expression of human SEPT7 Gmut1 fused to monomeric superfolder GFP

   Depositor

   Manos Mavrakis

   Article

   Martins et al J Cell Biol. 2023 Mar 6;222(3):e20220301

   Insert

   SEPTIN7 (SEPTIN7 Human)
   

   Use

   Tags

   msfGFP

   Expression

   Mammalian

   Mutation

   SEPT7 W269A, H279D

   Promoter

   CMV

   Available since

   Dec. 1, 2022

   Availability

   Academic Institutions and Nonprofits only

   

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6. pCMV_SEPT9_i3-mApple-CAAX

   Plasmid

   #180324

   Purpose

   mammalian expression of human SEPT9_i3 fused to monomeric Apple and CAAX (HRas)

   Depositor

   Manos Mavrakis

   Article

   Martins et al J Cell Biol. 2023 Mar 6;222(3):e20220301

   Insert

   SEPTIN9_v3 (SEPTIN9 Human)
   

   Use

   Tags

   mApple

   Expression

   Mammalian

   Mutation

   Promoter

   CMV

   Available since

   Dec. 1, 2022

   Availability

   Academic Institutions and Nonprofits only

   

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7. pCMV_SEPT9_i3 NCmut-msfGFP

   Plasmid

   #180325

   Purpose

   mammalian expression of human SEPT9_i3 NCmut fused to monomeric superfolder GFP

   Depositor

   Manos Mavrakis

   Article

   Martins et al J Cell Biol. 2023 Mar 6;222(3):e20220301

   Insert

   SEPTIN9_v3 (SEPTIN9 Human)
   

   Use

   Tags

   msfGFP

   Expression

   Mammalian

   Mutation

   SEPT9_i3 I263D, M270D

   Promoter

   CMV

   Available since

   Dec. 1, 2022

   Availability

   Academic Institutions and Nonprofits only

   

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8. pAB(EXPR-DS-HDAC5_HDAC3)

   Plasmid

   #114399

   Purpose

   For PYL-HDAC5 catalytic domain swap to HDAC3 catalytic domain expression

   Depositor

   Vincent Dion

   Article

   Yang et al Hum Mol Genet. 2021 Sep 7. pii: 6365982.

   Insert

   PYL-HDAC5_HDAC3 catalytic domain swap (HDAC3, HDAC5 Human)
   

   Use

   Tags

   3xFlag-NLS (internal)

   Expression

   Mammalian

   Mutation

   Promoter

   CMV

   Available since

   Oct. 13, 2021

   Availability

   Academic Institutions and Nonprofits only

   

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9. pNB1-LOX

   Plasmid

   #154058

   Purpose

   Level 1, Position 3 Golden Gate vector. ZmUbi-5'UTR:loxP-GUS-nosT-loxP-NAM-B1-nosT

   Depositor

   Cristobal Uauy

   Article

   Harrington et al Plant Methods. 2020 Oct 14;16:137. doi:

   Insert

   LoxP-flanked GUS and TtNAM-B1
   

   Use

   Synthetic Biology

   Tags

   Expression

   Bacterial

   Mutation

   The TtNAM-B1 gene sequence was domesticated to re…

   Promoter

   ZmUbi

   Available since

   Sept. 29, 2020

   Availability

   Academic Institutions and Nonprofits only

   

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10. RALA V25L

    Plasmid

    #122927

    Purpose

    Bacterial expression of RALA V25L

    Depositor

    Greg Cooper

    Article

    Hiatt et al PLoS Genet. 2018 Nov 30;14(11):e1007671.

    Insert

    RALA (RALA Human)
    

    Use

    Tags

    His

    Expression

    Bacterial

    Mutation

    V25L

    Promoter

    T7

    Available since

    April 4, 2019

    Availability

    Academic Institutions and Nonprofits only

    

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11. RALA V25M

    Plasmid

    #122928

    Purpose

    Bacterial expression of RALA V25M

    Depositor

    Greg Cooper

    Article

    Hiatt et al PLoS Genet. 2018 Nov 30;14(11):e1007671.

    Insert

    RALA (RALA Human)
    

    Use

    Tags

    His

    Expression

    Bacterial

    Mutation

    V25M

    Promoter

    T7

    Available since

    April 4, 2019

    Availability

    Academic Institutions and Nonprofits only

    

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12. RALA D130G

    Plasmid

    #122929

    Purpose

    Bacterial expression of RALA D130G

    Depositor

    Greg Cooper

    Article

    Hiatt et al PLoS Genet. 2018 Nov 30;14(11):e1007671.

    Insert

    RALA (RALA Human)
    

    Use

    Tags

    His

    Expression

    Bacterial

    Mutation

    D130G

    Promoter

    T7

    Available since

    April 4, 2019

    Availability

    Academic Institutions and Nonprofits only

    

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13. RALA S157A

    Plasmid

    #122930

    Purpose

    Bacterial expression of RALA S157A

    Depositor

    Greg Cooper

    Article

    Hiatt et al PLoS Genet. 2018 Nov 30;14(11):e1007671.

    Insert

    RALA (RALA Human)
    

    Use

    Tags

    His

    Expression

    Bacterial

    Mutation

    S157A

    Promoter

    T7

    Available since

    April 4, 2019

    Availability

    Academic Institutions and Nonprofits only

    

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14. RALA R176X

    Plasmid

    #122931

    Purpose

    Bacterial expression of RALA R176X

    Depositor

    Greg Cooper

    Article

    Hiatt et al PLoS Genet. 2018 Nov 30;14(11):e1007671.

    Insert

    RALA (RALA Human)
    

    Use

    Tags

    His

    Expression

    Bacterial

    Mutation

    R176X

    Promoter

    T7

    Available since

    April 4, 2019

    Availability

    Academic Institutions and Nonprofits only

    

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15. RALA WT

    Plasmid

    #122925

    Purpose

    Bacterial expression of RALA WT

    Depositor

    Greg Cooper

    Article

    Hiatt et al PLoS Genet. 2018 Nov 30;14(11):e1007671.

    Insert

    RALA (RALA Human)
    

    Use

    Tags

    His

    Expression

    Bacterial

    Mutation

    Promoter

    T7

    Available since

    April 4, 2019

    Availability

    Academic Institutions and Nonprofits only

    

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16. RALA G23D

    Plasmid

    #122926

    Purpose

    Bacterial expression of RALA G23D

    Depositor

    Greg Cooper

    Article

    Hiatt et al PLoS Genet. 2018 Nov 30;14(11):e1007671.

    Insert

    RALA (RALA Human)
    

    Use

    Tags

    His

    Expression

    Bacterial

    Mutation

    G23D

    Promoter

    T7

    Available since

    April 4, 2019

    Availability

    Academic Institutions and Nonprofits only

    

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17. pcDNA3.1-nAChr-Alpha6-CFP

    Plasmid

    #50482

    Purpose

    this report is the first to directly measure nAChr subunit stoichiometry using FRET and plasma membrane localization of Alpha6 and Beta3 containing receptors using TIRF

    Depositor

    Henry Lester

    Article

    Drenan et al Mol Pharmacol. 2008 Jan;73(1):27-41. Epu

    Insert

    nAChr-alpha6 (Chrna6 Mouse)
    

    Use

    Tags

    CFP fusion in M3-M4 loop (after residue A405)

    Expression

    Mammalian

    Mutation

    Gly-Ala-Gly flexible linker flanking the CFP open…

    Promoter

    CMV

    Available since

    Jan. 13, 2014

    Availability

    Academic Institutions and Nonprofits only

18. pSIN-TRE-H2BeGFP-rtTA2

    Plasmid

    #165494

    Purpose

    Doxycycline-inducible all-in-one lentivirus vector to express H2BeGFP fusion protein. System for labelling slow-cycling cells in vivo.

    Depositor

    Hector G. Palmer

    Article

    Puig et al J Clin Invest. 2018 Aug 31;128(9):3887-3

    Insert

    Human histone H2B (H2BC21 Human)
    

    Use

    Lentiviral

    Tags

    EGFP

    Expression

    Mutation

    -

    Promoter

    TetO-CMV

    Available since

    March 10, 2021

    Availability

    Academic Institutions and Nonprofits only

    

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19. MSCV-N E1B 19k

    Plasmid

    #38047

    Depositor

    Karl Munger

    Article

    Rozenblatt-Rosen et al Nature. 2012 Jul 26;487(7408):491-5.

    Insert

    E1B 19K
    

    Use

    Retroviral

    Tags

    Flag and HA

    Expression

    Mammalian

    Mutation

    Promoter

    PKG

    Available since

    Aug. 3, 2012

    Availability

    Academic Institutions and Nonprofits only

20. Ai162 (TIT2L-GC6s-ICL-tTA2) targeting vector

    Plasmid

    #114433

    Purpose

    Target a Cre-dependent GCaMP6s cassette and a tTA2 cassette into the mouse TIGRE locus

    Depositor

    Hongkui Zeng

    Article

    Daigle et al Cell. 2018 Jul 12;174(2):465-480.e22. do

    Insert

    GCaMP6s, tTA2
    

    Use

    Cre/Lox and Mouse Targeting

    Tags

    Expression

    Mutation

    Promoter

    TRE2, CAG

    Available since

    Jan. 7, 2019

    Availability

    Academic Institutions and Nonprofits only

    

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