166,818 results

1. OpenCRISPR-1 sgRNA-12nt stem

   Plasmid

   #221567

   Purpose

   Express OpenCRISPR-1 sgRNA (12nt stem loop) in human cells using a U6 promoter. Plasmid also encodes a CMV-driven GFP reporter.

   Depositor

   Ali Madani

   Article

   Ruffolo et al Nature. 2025 Jul 30. doi: 10.1038/s41586

   Insert

   OpenCRISPR-1 sgRNA 12nt stem loop
   

   Use

   Tags

   Expression

   Mammalian

   Mutation

   Promoter

   U6

   Available Since

   Aug. 28, 2024

   Availability

   Industry, Academic Institutions, and Nonprofits

   

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2. pGP-AAV-syn-jGCaMP8s-WPRE (AAV Retrograde)

   Viral Prep

   #162374-AAVrg

   Purpose

   Ready-to-use AAV Retrograde particles produced from pGP-AAV-syn-jGCaMP8s-WPRE (#162374). In addition to the viral particles, you will also receive purified pGP-AAV-syn-jGCaMP8s-WPRE plasmid DNA. Syn-driven expression of calcium sensor GCaMP8s (more sensitive). These AAV were produced with a retrograde serotype, which permits retrograde access to projection neurons. These AAV preparations are suitable purity for injection into animals.

   Depositor

   GENIE Project

   Article

   Zhang et al Janelia Research Campus, Online Resource

   Promoter

   Syn

   Tags

   None

   Available Since

   June 22, 2021

   Availability

   Academic Institutions and Nonprofits only

3. pHAGE TRE dCas9-KRAB

   Plasmid

   #50917

   Purpose

   Tet-regulatable dCas9-KRAB 2nd generation lentiviral expression vector

   Depositor

   Rene Maehr, Scot Wolfe

   Article

   Kearns et al Development. 2014 Jan;141(1):219-23. doi

   Insert

   dCas9
   

   Use

   CRISPR and Lentiviral

   Tags

   3xHA and KRAB

   Expression

   Mammalian

   Mutation

   D10A, H840A (catalytically inactive)

   Promoter

   TRE

   Available Since

   Feb. 11, 2014

   Availability

   Academic Institutions and Nonprofits only

   

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4. GFP-C1-PLCdelta-PH

   Plasmid

   #21179

   Purpose

   encodes a PI(4,5)P2 lipid selective PH domain that can be used as a fluorescent translocation biosensor to monitor changes or local differences in the concentration of plasma membrane PI(4,5)P2 lipids

   Depositor

   Tobias Meyer

   Article

   Stauffer et al Curr Biol. 1998 Mar 12. 8(6):343-6.

   Insert

   PLCdelta (PLCD1 Human)
   

   Use

   Tags

   GFP

   Expression

   Mammalian

   Mutation

   PH domain of human PLC delta, amino acids Met1–Il…

   Promoter

   Available Since

   July 10, 2009

   Availability

   Academic Institutions and Nonprofits only

   

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5. SPLICS Mt-ER Long P2A

   Plasmid

   #164107

   Purpose

   Detect the long Mitochondria-Endoplasmic reticulum contact

   Depositor

   Marisa Brini, Tito Calì

   Article

   Vallese et al Nat Commun. 2020 Nov 27;11(1):6069. doi:

   Insert

   Split GFP Mt-ER Long P2A
   

   Use

   Tags

   Expression

   Mammalian

   Mutation

   Promoter

   Available Since

   Jan. 25, 2021

   Availability

   Academic Institutions and Nonprofits only

   

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6. Cytoplasmic PancACe

   Plasmid

   #215708

   Purpose

   Expresses acetyl-CoA biosensor in the cytoplasm

   Depositor

   Katharine Diehl

   Article

   Smith et al Cell Chem Biol. 2025 Jan 21:S2451-9456(2

   Insert

   PancACe
   

   Use

   Lentiviral

   Tags

   FLAG, HA, and cytoplasmic localization sequence

   Expression

   Mammalian

   Mutation

   Promoter

   CMV

   Available Since

   March 12, 2024

   Availability

   Academic Institutions and Nonprofits only

   

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7. pCS2+MLS-HyPer7

   Plasmid

   #136470

   Purpose

   Mammalian expression of mitochondrial matrix targeted ultrasensitive hydrogen peroxide indicator HyPer7 for optical imaging

   Depositor

   Vsevolod Belousov

   Article

   Pak et al Cell Metab. 2020 Mar 3;31(3):642-653.e6.

   Insert

   HyPer7
   

   Use

   Tags

   Tandem mitochondrial targeting signal of cytochro…

   Expression

   Mammalian

   Mutation

   Promoter

   CMV, SP6

   Available Since

   Feb. 11, 2020

   Availability

   Academic Institutions and Nonprofits only

   

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8. pCAG-TetON-3G

   Plasmid

   #96963

   Purpose

   Mammalian expression of Clontech rtTA driven by human beta-actin promoter

   Depositor

   Elena Cattaneo

   Article

   Faedo et al Proc Natl Acad Sci U S A. 2017 Feb 14;11

   Type

   Empty backbone

   Use

   Tags

   Expression

   Mammalian

   Mutation

   Promoter

   Available Since

   Aug. 1, 2017

   Availability

   Academic Institutions and Nonprofits only

   

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9. pcDNA3 HA-Sumo2 WT

   Plasmid

   #48967

   Purpose

   Expresses HA SUMO2 Wild Type in mammalian cells

   Depositor

   Guy Salvesen

   Article

   Bekes et al J Biol Chem. 2011 Mar 25;286(12):10238-4

   Insert

   SUMO2 (SUMO2 Human)
   

   Use

   Tags

   HA

   Expression

   Mammalian

   Mutation

   Promoter

   T7

   Available Since

   Oct. 23, 2013

   Availability

   Academic Institutions and Nonprofits only

   

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10. pLentiCRISPR-EGFP

    Plasmid

    #75159

    Purpose

    Derived from LentiCRISPR v1 but expresses EGFP instead of puromycin resistance

    Depositor

    Beat Bornhauser

    Article

    McComb et al Sci Transl Med. 2016 May 18;8(339):339ra

    Type

    Empty backbone

    Use

    CRISPR and Lentiviral

    Tags

    EGFP

    Expression

    Mammalian

    Mutation

    Promoter

    Available Since

    May 17, 2016

    Availability

    Academic Institutions and Nonprofits only

    

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11. pAAV-EWB-DIO-myrTagRFP-T-P2A-post-eGRASP

    Plasmid

    #111581

    Purpose

    An AAV vector that expresses double floxed myristoylated TagRFP-T and post-eGRASP linked by self-cleaving P2A peptide under the Ef1a promoter.

    Depositor

    Bong-Kiun Kaang

    Article

    Choi et al Science. 2018 Apr 27;360(6387):430-435.

    Insert

    myrTagRFP-T-P2A-post-eGRASP
    

    Use

    AAV

    Tags

    Expression

    Mutation

    Promoter

    Ef1a

    Available Since

    June 12, 2018

    Availability

    Academic Institutions and Nonprofits only

    

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12. pLVX-UbC-rtTA-Ngn2:2A:EGFP

    Plasmid

    #127288

    Purpose

    all-in-one inducible LV expression construct for Ngn2:2A:EGFP (aka. UNG; for direct neuronal conversion)

    Depositor

    Fred Gage

    Article

    Schafer et al Nat Neurosci. 2019 Feb;22(2):243-255. do

    Insert

    Ngn2:2A:Ascl1 (NEUROG2 Human)
    

    Use

    Lentiviral

    Tags

    Ngn2 and EGFP linked via 2A

    Expression

    Mammalian

    Mutation

    Promoter

    Available Since

    June 6, 2019

    Availability

    Academic Institutions and Nonprofits only

    

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13. Go1-CASE

    Plasmid

    #168123

    Purpose

    Encodes a BRET-based activity sensor for the heterotrimeric G protein Go1. Composed of the subunits G alpha o1 (GNAO1) tagged with NanoLuciferase, G beta 3 (GNB3) and Venus-tagged G gamma 9 (GNG9).

    Depositor

    Gunnar Schulte

    Article

    Schihada et al Sci Signal. 2021 Sep 7;14(699):eabf1653.

    Insert

    GNAO1, GNB3, GNG9 (GNAO1, GNB3, GNGT2 Human)
    

    Use

    Luciferase

    Tags

    cpVenus on GNG9

    Expression

    Mammalian

    Mutation

    NLuc is inserted at L91/G92 within GNAO1

    Promoter

    Available Since

    July 29, 2021

    Availability

    Academic Institutions and Nonprofits only

    

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14. pT7-PEmax for IVT

    Plasmid

    #178113

    Purpose

    Template for in vitro transcription of PEmax

    Depositor

    David Liu

    Article

    Chen et al Cell. 2021 Oct 28;184(22):5635-5652.e29.

    Insert

    PEmax
    

    Use

    Template for in vitro transcription

    Tags

    SV40 bpNLS and c-Myc NLS

    Expression

    Mutation

    Detailed in manuscript

    Promoter

    T7 (inactivated)

    Available Since

    Nov. 30, 2021

    Availability

    Academic Institutions and Nonprofits only

    

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15. pR26 CAG/GFP Asc

    Plasmid

    #74285

    Purpose

    Gene targeting vector for the mouse Rosa26 locus, including a CAG promoter, loxP flanked stop cassette and GFP, for cloning into AscI

    Depositor

    Ralf Kuehn

    Article

    Chu et al BMC Biotechnol. 2016 Jan 16;16(1):4. doi

    Insert

    Rosa26 5-homology region
    

    Use

    Mouse Targeting

    Tags

    Expression

    Mutation

    Promoter

    Available Since

    April 15, 2016

    Availability

    Academic Institutions and Nonprofits only

    

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16. pTn Donor

    Plasmid

    #236211

    Purpose

    Arabinose-inducible Tn5 InducTn-seq plasmid

    Depositor

    Matthew Waldor

    Article

    Basta et al Nat Microbiol. 2025 Mar 27. doi: 10.1038

    Insert

    None
    

    Use

    Tags

    Expression

    Bacterial

    Mutation

    Promoter

    Available Since

    April 15, 2025

    Availability

    Academic Institutions and Nonprofits only

    

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17. pcDNA3.1 iCasper T2A HO1

    Plasmid

    #64278

    Purpose

    Expresses infrared fluorescent executioner caspase reporter (iCasper) plus human heme oxygenase 1

    Depositor

    Xiaokun Shu

    Article

    To et al Proc Natl Acad Sci U S A. 2015 Mar 17;11

    Insert

    Infrared fluorescent executioner caspase reporter (iCasper) T2A heme oxygenase-1: (HMOX1 Synthetic)
    

    Use

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    CMV

    Available Since

    April 24, 2015

    Availability

    Academic Institutions and Nonprofits only

    

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18. M43: pHR-SFFVp-iLID::EGFP::FTH1

    Plasmid

    #122149

    Purpose

    Self-assebled 24-mer tagged with iLID, and EGFP. Upon blue-light activation can bind up to 24 SspB-modified proteins.

    Depositor

    Clifford Brangwynne

    Article

    Bracha et al Cell. 2018 Nov 29;175(6):1467-1480.e13.

    Insert

    FTH1 (FTH1 Human)
    

    Use

    Lentiviral

    Tags

    NLS-iLID-GFP

    Expression

    Mammalian

    Mutation

    Promoter

    SFFV

    Available Since

    March 22, 2019

    Availability

    Academic Institutions and Nonprofits only

    

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19. pLenti-CMV-Puro-AAVR-FLAG

    Plasmid

    #166716

    Purpose

    Vector expressing AAVR (KIAA0319L) with a C-terminal Flag tag

    Depositor

    Jan Carette

    Article

    Pillay et al Nature. 2016 Feb 4;530(7588):108-12. doi

    Insert

    AAVR-FLAG (KIAA0319L Human)
    

    Use

    Lentiviral

    Tags

    FLAG

    Expression

    Mammalian

    Mutation

    Promoter

    CMV

    Available Since

    June 15, 2021

    Availability

    Academic Institutions and Nonprofits only

    

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20. AAV-CAG-flex-GFP-4x6T

    Plasmid

    #196418

    Purpose

    Cre-dependent AAV expression of GFP preferentially in astrocytes; astrocyte selectivity generated with 4x6T miRNA targeting cassette

    Depositor

    Stanley Thomas Carmichael

    Article

    Gleichman et al Nat Commun. 2023 Nov 16;14(1):7426. doi:

    Insert

    GFP
    

    Use

    AAV and Cre/Lox; Astrocyte-selective

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    CAG

    Available Since

    Feb. 23, 2023

    Availability

    Academic Institutions and Nonprofits only

    

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