We narrowed to 6,993 results for: Cad

1. pcDNA4-ATR-C-KD

   Plasmid

   #53770

   Purpose

   mammalian expression of flag-tagged ATR C-terminal fragment kinase dead mutant

   Depositor

   Aziz Sancar

   Article

   Unsal-Kacmaz et al Proc Natl Acad Sci U S A. 2002 May 14;99

   Insert

   ATR (ATR Human)
   

   Use

   Tags

   Flag

   Expression

   Mammalian

   Mutation

   C-terminal domain, aa 1398-2644, and kinase dead …

   Promoter

   CMV

   Available since

   July 8, 2014

   Availability

   Academic Institutions and Nonprofits only

2. pINDUCER13 (miR-LUP)

   Plasmid

   #46936

   Purpose

   Inducible lentiviral gene silencing vector. Insert PheSGly294, (XhoI to EcoRI; 1.4kb), can be replaced w mir30 based hairpin of interest

   Depositor

   Thomas Westbrook

   Article

   Meerbrey et al Proc Natl Acad Sci U S A. 2011 Mar 1;108

   Insert

   PheS Gly294
   

   Use

   Lentiviral

   Tags

   Expression

   Mutation

   Promoter

   Available since

   Nov. 4, 2014

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

3. pMax_mPlum_C207G/T208C

   Plasmid

   #177831

   Purpose

   Site-specific mutagenesis of mPlum for the purposes of creating a site-specific O6-MeG lesion for the purposes of measuring MGMT activity via Host Cell Reactivation (HCR).

   Depositor

   Leona Samson, Zachary Nagel

   Article

   Nagel et al Proc Natl Acad Sci U S A. 2014 May 6;111

   Insert

   mPlum_C207G/T208C
   

   Use

   Tags

   Expression

   Mammalian

   Mutation

   C207G + T208C

   Promoter

   CMV

   Available since

   Jan. 3, 2023

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

4. EE-LRRK2

   Plasmid

   #193583

   Purpose

   Expresses full-length LRRK2 with FYVE-CC2 domains from HRS and 3xFLAG on the N-terminus

   Depositor

   Mark Cookson

   Article

   Kluss et al Proc Natl Acad Sci U S A. 2022 Oct 25;11

   Insert

   Leucine-rich repeat kinase 2 (LRRK2 Human)
   

   Use

   Tags

   FYVE-CC2 and 3xFLAG

   Expression

   Mammalian

   Mutation

   Promoter

   CMV

   Available since

   Jan. 17, 2023

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

5. J23104-mRFP1-331Bb

   Plasmid

   #78274

   Purpose

   mRFP1 behind constitutive promoter J23104 in pSEVA331Bb backbone

   Depositor

   Tom Ellis

   Article

   Florea et al Proc Natl Acad Sci U S A. 2016 Jun 14;11

   Insert

   J23104-mRFP1
   

   Use

   Tags

   Expression

   Mutation

   Promoter

   Available since

   Nov. 10, 2016

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

6. pQ-NFY-A-GB-HC

   Plasmid

   #171081

   Purpose

   Co-expresses of NFY complex in bacteria. The resulting plasmid was used to generate a single expression construct encoding the three subunit NF-Y, with untagged NFYA, GST-NFYB, and 6-His-NFYC

   Depositor

   Joan Conaway

   Article

   He et al Proc Natl Acad Sci U S A. 2021 Sep 28;11

   Inserts

   NF-YA (NFYA Human)
   NF-YB (NFYB Human)
   NF-YC (NFYC Human)
   

   Use

   Tags

   GST and His

   Expression

   Bacterial

   Mutation

   Promoter

   T5 promoter

   Available since

   July 19, 2024

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

7. pTwist-SFFV-NFE2L18ND-Puro

   Plasmid

   #181918

   Purpose

   Lentiviral plasmid that directs the expression of mutant NFE2L1/Nrf1 where 8 putative N-glycosites are mutated from asparagine to aspartic acid in mammalian cells.

   Depositor

   Scott Dixon

   Article

   Forcina et al Proc Natl Acad Sci U S A. 2022 Mar 15;11

   Insert

   NFE2L1 (NFE2L1 Human)
   

   Use

   Lentiviral

   Tags

   FLAG and HA

   Expression

   Mammalian

   Mutation

   8 putative N-glycoyslation sites converted from a…

   Promoter

   SFFV

   Available since

   April 6, 2022

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

8. MAP4K3-GFP

   Plasmid

   #21731

   Depositor

   L. Miguel Martins

   Article

   Lam et al Proc Natl Acad Sci U S A. 2009 Jul 21;10

   Insert

   MAP4K3 (MAP4K3 Human)
   

   Use

   Tags

   GFP

   Expression

   Mammalian

   Mutation

   Promoter

   Available since

   Aug. 11, 2009

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

9. pcDNA3-GFP-LpIA(wild-type)

   Plasmid

   #61821

   Purpose

   Encodes a wild-type sequence of LplA and serves as the negative control for resorufin labeling

   Depositor

   Alice Ting

   Article

   Liu et al Proc Natl Acad Sci U S A. 2014 Oct 28;11

   Insert

   E. coli lipoic acid ligase
   

   Use

   Tags

   6XHis, EGFP, and Flag

   Expression

   Mammalian

   Mutation

   Promoter

   CMV

   Available since

   Aug. 25, 2015

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

10. pXPR_023d-sgCiCh2-2

    Plasmid

    #202444

    Purpose

    Expression of a CRISPRi control sgRNA that cuts an intergenic region on chromosome 2

    Depositor

    William Hahn

    Article

    Cervia et al Cancer Discov. 2023 Mar 1;13(3):766-795.

    Insert

    Chromosome 2 gRNA
    

    Use

    CRISPR and Lentiviral

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    hU6

    Available since

    June 30, 2023

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

11. pLM-mCitrine-Sox2

    Plasmid

    #23242

    Depositor

    Michel Sadelain

    Article

    Papapetrou et al Proc Natl Acad Sci U S A. 2009 Aug 4. 10

    Insert

    mCitrine_2A_SOX2 (SOX2 Human, synthetic fluorescent protein)
    

    Use

    Lentiviral

    Tags

    Expression

    Mutation

    Promoter

    Available since

    Feb. 5, 2010

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

12. Tol2-lyzC-mcherry-2a-CDK2

    Plasmid

    #121131

    Purpose

    neutrophil specific expression

    Depositor

    Qing Deng

    Article

    Hsu et al Proc Natl Acad Sci U S A. 2019 Aug 26. p

    Insert

    dre-WT CDK2 (cdk2 Zebrafish)
    

    Use

    Tags

    mcherry-2a

    Expression

    Bacterial

    Mutation

    Promoter

    neutrophil specific lyzC

    Available since

    Sept. 9, 2019

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

13. pCMV4Neo-murine IL-17RC(FL)

    Plasmid

    #46864

    Purpose

    expresses mouse IL-17RC (all exons)

    Depositor

    Sarah Gaffen

    Article

    Maitra et al Proc Natl Acad Sci U S A. 2007 May 1;104

    Insert

    IL-17RC (Il17rc Mouse)
    

    Use

    Tags

    Myc

    Expression

    Mammalian

    Mutation

    Promoter

    CMV

    Available since

    Sept. 11, 2013

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

14. pAPM-miR30-MAVS-ts3

    Plasmid

    #174276

    Purpose

    MAVS knockdown

    Depositor

    Jeremy Luban

    Article

    Guney et al Proc Natl Acad Sci U S A. 2024 Jul 16;12

    Insert

    MAVS shRNA (MAVS Human)
    

    Use

    Lentiviral and RNAi

    Tags

    Expression

    Mutation

    Promoter

    SFFV

    Available since

    Aug. 27, 2024

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

15. pTMiniTrex-mCherry-Shark2

    Plasmid

    #233144

    Purpose

    Serves as a template plasmid for amplifying Shark2 (Tet-ON) hammerhead aptazyme-3xTy-tagging cassette

    Depositor

    Ronald Etheridge

    Article

    Wiedeman et al Proc Natl Acad Sci U S A. 2025 Feb 25;12

    Inserts

    mCherry
    Shark2
    

    Use

    Prokaryotic expression

    Tags

    3x Ty tag

    Expression

    Mutation

    Codon optimized for Trypanosoma cruzi

    Promoter

    Available since

    March 20, 2025

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

16. pAdTrack-CMV HA ALAS-1

    Plasmid

    #22746

    Depositor

    Bruce Spiegelman

    Article

    Estall et al Proc Natl Acad Sci U S A. 2009 Dec 29. 1

    Insert

    ALAS-1 (Alas1 Mouse)
    

    Use

    Adenoviral

    Tags

    HA

    Expression

    Mammalian

    Mutation

    Promoter

    Available since

    June 2, 2010

    Availability

    Academic Institutions and Nonprofits only

17. pCMV-FLAG-LOV*-MVP

    Plasmid

    #202206

    Purpose

    Expresses the photoproximity labeling catalyst LOV* fused to MVP in mammalian cells

    Depositor

    Thomas Muir

    Article

    Hananya et al Proc Natl Acad Sci U S A. 2023 Apr 18;12

    Insert

    Major vault protein (MVP Human)
    

    Use

    Tags

    FLAG and LOV*

    Expression

    Mammalian

    Mutation

    Promoter

    CMV

    Available since

    July 26, 2023

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

18. pLINK cyclin T1 (1-280) myc (P#586)

    Plasmid

    #14612

    Depositor

    Matija Peterlin

    Article

    Fujinaga et al Proc Natl Acad Sci U S A. 1999 Feb 16. 9

    Insert

    cyclin T1 (1-280) (CCNT1 Human)
    

    Use

    Tags

    myc

    Expression

    Mammalian

    Mutation

    Promoter

    Available since

    June 30, 2008

    Availability

    Academic Institutions and Nonprofits only

19. pFRLuc-CoV2-delStem1

    Plasmid

    #177616

    Purpose

    Dual luciferase reporter for SARS-CoV-2 programmed -1 ribosomal frameshifting. 3-nucleotide deletion in Stem 1 (negative control).

    Depositor

    Junjie Guo

    Article

    Sun et al Proc Natl Acad Sci U S A. 2021 Jun 29;11

    Insert

    SARS-CoV-2 FSE
    

    Use

    Luciferase

    Tags

    Expression

    Mammalian

    Mutation

    3-nucleotide (UAC) deletion in Stem 1

    Promoter

    Available since

    Dec. 10, 2021

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

20. Chr7_Centromere-Targeting_gRNA

    Plasmid

    #195129

    Purpose

    dual gRNA vector targeting centromere-proximal locations on Chromosome 7p and 7q in a third generation Cas9 backbone with GFP

    Depositor

    Jason Sheltzer

    Article

    Girish et al Science. 2023 Jul 6:eadg4521. doi: 10.11

    Insert

    Chr7 gRNA
    

    Use

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    Available since

    Feb. 6, 2023

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

21. Cav2.2 BBS pcDNA3

    Plasmid

    #206057

    Purpose

    expression of rabbit Cav2.2 calcium channel with an exofacial double Bungarotoxin Binding Site motif in domain II

    Depositor

    Annette Dolphin

    Article

    Cassidy et al Proc Natl Acad Sci U S A. 2014 Jun 17;11

    Insert

    cacna1b (CACNA1B Rabbit)
    

    Use

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    CMV

    Available since

    Sept. 14, 2023

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

22. pTSlb-N748D

    Plasmid

    #60732

    Purpose

    Expresses both fragments of T7 RNAP split at position 179. The N-terminal fragment is driven by PLac while the C-terminal fragment is driven by PBAD. C-terminal fragment has the point mutations N748D.

    Depositor

    Matthew Bennett

    Article

    Shis et al Proc Natl Acad Sci U S A. 2013 Mar 26;11

    Inserts

    Residues 1-179 of split T7 RNAP
    Residues 180-880 of split T7 RNAP
    

    Use

    Synthetic Biology

    Tags

    Expression

    Bacterial

    Mutation

    Residues 1-180 of split T7 RNAP and Residues 180-…

    Promoter

    Available since

    Nov. 5, 2014

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

23. pBSFI-myr-akt-T308A/S473A

    Plasmid

    #49191

    Purpose

    cDNA of Akt1 with myristyolation signal and point mutation from T to A at position 308 and S to A at position 473 in cloning vector

    Depositor

    Peter Vogt

    Article

    Aoki et al Proc Natl Acad Sci U S A. 1998 Dec 8;95(

    Insert

    Akt1 (Akt1 Mouse)
    

    Use

    Adaptor plasmid

    Tags

    HA tag and myristolation signal

    Expression

    Mutation

    T308A; S473A

    Promoter

    T7

    Available since

    Dec. 13, 2013

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

24. pMXs-hLIN28A

    Plasmid

    #47902

    Purpose

    Expresses human LIN28A in mammalian cells using retrovirus system.

    Depositor

    Shinya Yamanaka

    Article

    Tanabe et al Proc Natl Acad Sci U S A. 2013 Jul 23;11

    Insert

    human LIN28A (LIN28A Human)
    

    Use

    Retroviral

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    LTR

    Available since

    Oct. 2, 2013

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

25. pAPM-miR30-MAVS-ts1

    Plasmid

    #174274

    Purpose

    MAVS knockdown

    Depositor

    Jeremy Luban

    Article

    Guney et al Proc Natl Acad Sci U S A. 2024 Jul 16;12

    Insert

    MAVS shRNA (MAVS Human)
    

    Use

    Lentiviral and RNAi

    Tags

    Expression

    Mutation

    Promoter

    SFFV

    Available since

    Aug. 27, 2024

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

26. 904 pLNCX HA Akt1 K179M

    Plasmid

    #9007

    Depositor

    William Sellers

    Article

    Ramaswamy et al Proc Natl Acad Sci U S A. 1999 Mar 2. 96

    Insert

    Akt1 K179M (AKT1 Human)
    

    Use

    Retroviral

    Tags

    HA

    Expression

    Mammalian

    Mutation

    K179M. Kinase dead.

    Promoter

    Available since

    Nov. 14, 2005

    Availability

    Academic Institutions and Nonprofits only

27. canxTM-mEmerald (cytosolic)

    Plasmid

    #186962

    Purpose

    Expresses mEmerald connected with the C-terminus of Calnexin transmembrane domain.

    Depositor

    Ke Xu

    Article

    Wang et al Proc Natl Acad Sci U S A. 2022 May 3;119

    Insert

    CANX (CANX Human)
    

    Use

    Tags

    mEmerald

    Expression

    Mammalian

    Mutation

    Only express calnexin (482-end) and add ER signal…

    Promoter

    CMV Promoter

    Available since

    Sept. 23, 2022

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

28. pENTR221-FGFR4

    Plasmid

    #60531

    Purpose

    Entry vector for human FGFR4

    Depositor

    John Brognard

    Article

    Fawdar et al Proc Natl Acad Sci U S A. 2013 Jul 23;11

    Insert

    FGFR4 (FGFR4 Human)
    

    Use

    Entry vector

    Tags

    Expression

    Mutation

    c2134a P712T

    Promoter

    NA

    Available since

    Feb. 18, 2015

    Availability

    Academic Institutions and Nonprofits only

29. pFastBac1-LRRK2 WD40

    Plasmid

    #139211

    Purpose

    Human LRRK2 WD40 domain (residues 2142–2527) was expressed in Sf9 insect cells via baculovirus infection and purified to homogeneity.

    Depositor

    Hao Wu

    Article

    Zhang et al Proc Natl Acad Sci U S A. 2019 Jan 29;11

    Insert

    LRRK2 (LRRK2 Human)
    

    Use

    Tags

    N-His and MBP

    Expression

    Insect

    Mutation

    Promoter

    Available since

    March 11, 2020

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

30. pMKO shRNA Bim

    Plasmid

    #17235

    Depositor

    Joan Brugge

    Article

    Schmelzle et al Proc Natl Acad Sci U S A. 2007 Mar 6. 10

    Insert

    Bim shRNA (BCL2L11 Human)
    

    Use

    RNAi and Retroviral

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    Available since

    Jan. 18, 2008

    Availability

    Academic Institutions and Nonprofits only