We narrowed to 31,407 results for: Eng

1. pMZ103-rbs1-m2

   Plasmid

   #204464

   Purpose

   This is a biosensor with improved specificity to 3OC12-HSL, containing LasR L128C mutation.

   Depositor

   Lauren Andrews

   Article

   Zeng et al ACS Synth Biol. 2024 Feb 16;13(2):568-58

   Inserts

   quorum sensing regulator LasR (lasR )
   eYFP
   

   Use

   Synthetic Biology

   Tags

   Expression

   Mutation

   LasR L128C mutation

   Promoter

   In an operon and PLas

   Available since

   March 5, 2025

   Availability

   Academic Institutions and Nonprofits only

   

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2. pmirGLO-mir302-8mer4x (pEZ185)

   Plasmid

   #229059

   Purpose

   Dual-luciferase reporter containing 4 copies of the 8mer site of human miR-302a

   Depositor

   S Chul Kwon

   Article

   Cheng et al EMBO Rep. 2025 Feb 26. doi: 10.1038/s443

   Insert

   Human miR-302a binding sites
   

   Use

   Tags

   Expression

   Mammalian

   Mutation

   Promoter

   Available since

   Feb. 26, 2025

   Availability

   Academic Institutions and Nonprofits only

   

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3. pCI5-CasRx-NLS-HA (pKW919)

   Plasmid

   #229047

   Purpose

   Mammalian expression of CasRx-NLS-HA

   Depositor

   S Chul Kwon

   Article

   Cheng et al EMBO Rep. 2025 Feb 26. doi: 10.1038/s443

   Insert

   CasRx (RfxCas13d)
   

   Use

   CRISPR

   Tags

   NLS and NLS-HA

   Expression

   Mammalian

   Mutation

   Promoter

   Available since

   Feb. 26, 2025

   Availability

   Academic Institutions and Nonprofits only

   

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4. pCI5-CasRx-NES-HA (pKW387)

   Plasmid

   #229048

   Purpose

   Mammalian expression of CasRx-NES-HA

   Depositor

   S Chul Kwon

   Article

   Cheng et al EMBO Rep. 2025 Feb 26. doi: 10.1038/s443

   Insert

   CasRx (RfxCas13d)
   

   Use

   CRISPR

   Tags

   NES-HA

   Expression

   Mammalian

   Mutation

   Promoter

   Available since

   Feb. 26, 2025

   Availability

   Academic Institutions and Nonprofits only

   

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5. EKAREN5-gl

   Plasmid

   #225958

   Purpose

   The YPet and mTurquoise sequence cassettes in EKAREN5(Addgene 167821) were replaced with a synonymous codon variant of YPet and mTurquoise-gl and subcloned into pCSII lentiviral backbone.

   Depositor

   Toru Hiratsuka

   Article

   Weisheng et al Cell Struct Funct. 2024 Dec 18. doi: 10.

   Insert

   EKAREN5-gl
   

   Use

   Lentiviral

   Tags

   nls localization motif

   Expression

   Mammalian

   Mutation

   Promoter

   EF-1-alpha promoter

   Available since

   Jan. 22, 2025

   Availability

   Academic Institutions and Nonprofits only

   

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6. pRS316-SSD1-L1374

   Plasmid

   #226278

   Purpose

   Plasmid expressing the SSD1 allele from L-1374, under control of its native promoter

   Depositor

   Jolanda Van Leeuwen

   Article

   Paltenghi et al G3 (Bethesda). 2025 Mar 3:jkaf047. doi:

   Insert

   SSD1 (SSD1 Budding Yeast)
   

   Use

   Tags

   Expression

   Yeast

   Mutation

   Promoter

   Available since

   Jan. 16, 2025

   Availability

   Academic Institutions and Nonprofits only

   

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7. pET29_anti-SARS-CoV-2-NM1226

   Plasmid

   #209422

   Purpose

   Express anti-SARS-CoV-2-Nanobody NM1226 in E. coli

   Depositor

   Magnus Rueping

   Article

   Peng et al ACS Synth Biol. 2025 Jan 9. doi: 10.1021

   Insert

   anti-SARS-CoV-2-NM1226
   

   Use

   Tags

   Myc-6xHis

   Expression

   Bacterial

   Mutation

   Promoter

   Available since

   Jan. 13, 2025

   Availability

   Industry, Academic Institutions, and Nonprofits

   

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8. pET29_anti-SARS-CoV-2-NM1230

   Plasmid

   #209423

   Purpose

   Express anti-SARS-CoV-2-Nanobody NM1230 in E. coli

   Depositor

   Magnus Rueping

   Article

   Peng et al ACS Synth Biol. 2025 Jan 9. doi: 10.1021

   Insert

   anti-SARS-CoV-2-NM1230
   

   Use

   Tags

   Myc-6xHis

   Expression

   Bacterial

   Mutation

   Promoter

   Available since

   Jan. 13, 2025

   Availability

   Industry, Academic Institutions, and Nonprofits

   

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9. pET29_anti-SARS-CoV-2-Nanobody-C5

   Plasmid

   #209420

   Purpose

   Express anti-SARS-CoV-2-Nanobody C5 in E. coli

   Depositor

   Magnus Rueping

   Article

   Peng et al ACS Synth Biol. 2025 Jan 9. doi: 10.1021

   Insert

   anti-SARS-CoV-2-Nanobody-C5
   

   Use

   Tags

   6xHis

   Expression

   Bacterial

   Mutation

   Promoter

   Available since

   Jan. 13, 2025

   Availability

   Industry, Academic Institutions, and Nonprofits

   

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10. pET29_anti-SARS-CoV-2-Nanobody-F2

    Plasmid

    #209421

    Purpose

    Express anti-SARS-CoV-2-Nanobody F2 in E. coli

    Depositor

    Magnus Rueping

    Article

    Peng et al ACS Synth Biol. 2025 Jan 9. doi: 10.1021

    Insert

    anti-SARS-CoV-2-Nanobody-F2
    

    Use

    Tags

    6xHis

    Expression

    Bacterial

    Mutation

    Promoter

    Available since

    Jan. 13, 2025

    Availability

    Industry, Academic Institutions, and Nonprofits

    

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11. pCambia3301-AtU6-MAR1

    Plasmid

    #210757

    Purpose

    Mutagenesis of the Arabidopsis MAR1 gene for efficient screening.

    Depositor

    Daisuke Miki

    Article

    Cheng et al Sci Rep. 2024 Dec 28;14(1):30791. doi: 1

    Insert

    AtU6-26 pro::MAR1 sgRNA
    

    Use

    CRISPR

    Tags

    Expression

    Plant

    Mutation

    Promoter

    AtU6-26

    Available since

    Nov. 26, 2024

    Availability

    Academic Institutions and Nonprofits only

    

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12. T2A GFP H2B

    Plasmid

    #227722

    Purpose

    Plasmid contains T2A GFP H2B, with some nlp-51 homology sequence before, Amp Resistant

    Depositor

    Haosheng Sun, Oliver Hobert

    Article

    Sun et al MicroPubl Biol. 2023 Nov 2;2023:10.17912

    Insert

    (nlp-51 homology) T2A 3xNLS GFP (nlp-51 Nematode)
    

    Use

    Cloning vector

    Tags

    T2A 3xNLS GFP

    Expression

    Mutation

    Promoter

    Available since

    Nov. 18, 2024

    Availability

    Academic Institutions and Nonprofits only

    

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13. T2A 3xNLS GFP

    Plasmid

    #227721

    Purpose

    Plasmid contains T2A 3xNLS GFP, with some nlp-51 homology sequence before, Amp Resistant

    Depositor

    Haosheng Sun, Oliver Hobert

    Article

    Sun et al MicroPubl Biol. 2023 Nov 2;2023:10.17912

    Insert

    T2A 3xNLS GFP with some nlp-51 homology sequence (nlp-51 Nematode)
    

    Use

    Cloning vector

    Tags

    Expression

    Mutation

    Promoter

    Available since

    Nov. 18, 2024

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

14. pRS315-SEC22-NCYC110

    Plasmid

    #226273

    Purpose

    Plasmid expressing the SEC22 allele from NCYC110, under control of its native promoter

    Depositor

    Jolanda Van Leeuwen

    Article

    Paltenghi et al G3 (Bethesda). 2025 Mar 3:jkaf047. doi:

    Insert

    SEC22 (SEC22 Budding Yeast)
    

    Use

    Tags

    Expression

    Yeast

    Mutation

    Promoter

    Available since

    Oct. 30, 2024

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

15. pRS315-SEC22-L1374

    Plasmid

    #226271

    Purpose

    Plasmid expressing the SEC22 allele from L-1374, under control of its native promoter

    Depositor

    Jolanda Van Leeuwen

    Article

    Paltenghi et al G3 (Bethesda). 2025 Mar 3:jkaf047. doi:

    Insert

    SEC22 (SEC22 Budding Yeast)
    

    Use

    Tags

    Expression

    Yeast

    Mutation

    Promoter

    Available since

    Oct. 30, 2024

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

16. pRS315-SEC22-UWOPS872421

    Plasmid

    #226272

    Purpose

    Plasmid expressing the SEC22 allele from UWOPS87-2421, under control of its native promoter

    Depositor

    Jolanda Van Leeuwen

    Article

    Paltenghi et al G3 (Bethesda). 2025 Mar 3:jkaf047. doi:

    Insert

    SEC22 (SEC22 Budding Yeast)
    

    Use

    Tags

    Expression

    Yeast

    Mutation

    Promoter

    Available since

    Oct. 30, 2024

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

17. pRS315-SEC22-S288C

    Plasmid

    #226270

    Purpose

    Plasmid expressing the SEC22 allele from S288C, under control of its native promoter

    Depositor

    Jolanda Van Leeuwen

    Article

    Paltenghi et al G3 (Bethesda). 2025 Mar 3:jkaf047. doi:

    Insert

    SEC22 (SEC22 Budding Yeast)
    

    Use

    Tags

    Expression

    Yeast

    Mutation

    Promoter

    Available since

    Oct. 30, 2024

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

18. pML107-PMR1

    Plasmid

    #226264

    Purpose

    Plasmid expressing Cas9 and gRNA ACATGACCGTATCTAAACTT which targets the PMR1 gene

    Depositor

    Jolanda Van Leeuwen

    Article

    Paltenghi et al G3 (Bethesda). 2025 Mar 3:jkaf047. doi:

    Insert

    PMR1 gRNA (PMR1 Budding Yeast)
    

    Use

    CRISPR

    Tags

    Expression

    Yeast

    Mutation

    Promoter

    Available since

    Oct. 28, 2024

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

19. pRS316-SSD1-NCYC110

    Plasmid

    #226279

    Purpose

    Plasmid expressing the SSD1 allele from NCYC110, under control of its native promoter

    Depositor

    Jolanda Van Leeuwen

    Article

    Paltenghi et al G3 (Bethesda). 2025 Mar 3:jkaf047. doi:

    Insert

    SSD1 (SSD1 Budding Yeast)
    

    Use

    Tags

    Expression

    Yeast

    Mutation

    Promoter

    Available since

    Oct. 28, 2024

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

20. pDLterm

    Plasmid

    #211903

    Purpose

    Base plasmid for dual-luciferase constructs to measure terminator strength

    Depositor

    Stan Fields

    Article

    Gorjifard et al Nat Commun. 2024 Jul 12;15(1):5868. doi:

    Type

    Empty backbone

    Use

    Luciferase

    Tags

    Expression

    Plant

    Mutation

    Promoter

    35S enhancer + 35S minimal promoter

    Available since

    Sept. 17, 2024

    Availability

    Academic Institutions and Nonprofits only

    

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21. Zhou-pLOV-GFP-Luc

    Plasmid

    #161030

    Purpose

    Lentiviral vector expressing GFP and luciferase for use as a reporter in pseudovirus production and infection.

    Depositor

    Dapeng Zhou

    Article

    Tong et al Vaccines (Basel). 2021 Mar 22;9(3). pii:

    Insert

    GFP and luciferase
    

    Use

    Lentiviral

    Tags

    Expression

    Mutation

    Promoter

    Available since

    July 26, 2024

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

22. pJH4693

    Plasmid

    #200784

    Purpose

    Ptwk-40s EGFP unc-54 3' UTR C.elegans AVA and other neurons expression of EGFP

    Depositor

    Mei Zhen

    Article

    Meng et al Sci Adv. 2024 Apr 12;10(15):eadk0002. do

    Insert

    no
    

    Use

    Tags

    GFP

    Expression

    Worm

    Mutation

    Promoter

    Ptwk-40s

    Available since

    May 7, 2024

    Availability

    Industry, Academic Institutions, and Nonprofits

    

    Enlarge

23. pJH4506

    Plasmid

    #200255

    Purpose

    Pnpr-4 TeTx mCherry unc-54 3' UTR C.elegans AVA and other neurons expression of TeTx RFP

    Depositor

    Mei Zhen

    Article

    Meng et al Sci Adv. 2024 Apr 12;10(15):eadk0002. do

    Insert

    TeTx
    

    Use

    Tags

    mCherry

    Expression

    Worm

    Mutation

    Promoter

    Pnpr-4

    Available since

    May 7, 2024

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

24. pJH1371

    Plasmid

    #199631

    Purpose

    Prgef-1 GPI YFP unc-54 3' UTR C.elegans Pan-neural expression of GPI-YFP

    Depositor

    Mei Zhen

    Article

    Meng et al Sci Adv. 2024 Apr 12;10(15):eadk0002. do

    Insert

    GPI
    

    Use

    Tags

    YFP

    Expression

    Worm

    Mutation

    Promoter

    Pregf-1

    Available since

    May 7, 2024

    Availability

    Industry, Academic Institutions, and Nonprofits

    

    Enlarge

25. pJH2673

    Plasmid

    #199691

    Purpose

    Pnmr-1 FLPe unc-54 3' UTR C.elegans AVA/E/D premotor IN and others expression of FLPe

    Depositor

    Mei Zhen

    Article

    Meng et al Sci Adv. 2024 Apr 12;10(15):eadk0002. do

    Insert

    flpE (flpE )
    

    Use

    Tags

    no

    Expression

    Worm

    Mutation

    Promoter

    Pnmr-1 long

    Available since

    May 7, 2024

    Availability

    Industry, Academic Institutions, and Nonprofits

    

    Enlarge

26. TtA_pET29b

    Plasmid

    #215432

    Purpose

    Expression construct for TtA gene, codon optimized for expression in E. coli

    Depositor

    Gregg Beckham

    Article

    Bell et al Nat Commun. 2024 Feb 9;15(1):1217. doi:

    Insert

    TtA
    

    Use

    Tags

    Expression

    Bacterial

    Mutation

    Promoter

    Available since

    April 29, 2024

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

27. pDonor_RB-TnV2_pJEx

    Plasmid

    #213908

    Purpose

    Barcoded mariner transposon with crystal violet-inducible outward promoter - barcodes can be added via FseI-SbfI restriction sites

    Depositor

    James Collins

    Article

    English et al Mol Syst Biol. 2023 Jun 12;19(6):e11398.

    Insert

    mariner transposon with kan resistance and EilR/pJEx and FseI-SbfI sites for barcode insertion
    

    Use

    Tags

    Expression

    Bacterial

    Mutation

    Promoter

    Available since

    Feb. 14, 2024

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

28. pBES1-LacOx50-mCh-bla

    Plasmid

    #210260

    Purpose

    Integrates 50xLacO repeats and an mCherry gene upstream of the BES1 promoter with a hygromycin drug selection marker downstream of the promoter.

    Depositor

    Calvin Tiengwe

    Article

    Budzak et al Mol Biochem Parasitol. 2023 Dec;256:1115

    Inserts

    50xLacO repeats derived from: PMID: 12864855.
    mCherry
    

    Use

    Tags

    Expression

    Mutation

    Promoter

    Available since

    Nov. 28, 2023

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

29. pGAL1-VP1 (LEU2)

    Plasmid

    #182480

    Purpose

    Yeast integrative plasmid for expressing deltaVP1, an NLS deletion mutant of Murine polyomavirus VP1 (GAL1 promoter). Contains leucine auxotrophic marker (K. lactis LEU2).

    Depositor

    Claudia Vickers

    Article

    Cheah et al bioRxiv 2022.11.24.517869

    Insert

    deltaVP1
    

    Use

    Synthetic Biology; Metabolic engineering

    Tags

    Expression

    Yeast

    Mutation

    Promoter

    GAL1

    Available since

    Dec. 6, 2022

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

30. pSLS.544

    Plasmid

    #184961

    Purpose

    Test effect of a1/a2 length on editing murF using retron recombineering

    Depositor

    Seth Shipman

    Article

    Lopez et al Nat Chem Biol. 2022 Feb;18(2):199-206. d

    Insert

    Eco1 RT and recombineering ncRNA, murF G1359A, a1/a2 length: 22
    

    Use

    Tags

    Expression

    Bacterial

    Mutation

    murF donor G1359A, a1/a2 length extended to 22 bp

    Promoter

    T7/lac

    Available since

    Nov. 4, 2022

    Availability

    Academic Institutions and Nonprofits only

    

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