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Plasmid#223418PurposeT-DNA vector for Mb2Cas12a-RVRR based mutagenesis for monocot plants; more relaxed PAM requirements; Mb2Cas12a-RVRR and the crRNA were driven by separate ZmUbi1; BASTA for plants selection.DepositorInsertZmUbi-Mb2Cas12a-RVRR-ZmUbi-crRNA scaffold
UseCRISPRTagsNLSExpressionPlantAvailable SinceApril 10, 2025AvailabilityAcademic Institutions and Nonprofits only -
GFP-nArgBP2
Plasmid#74514PurposeExpress GFP-nArgBP2 fusion protein in mammalian cellsDepositorAvailable SinceJuly 20, 2016AvailabilityAcademic Institutions and Nonprofits only -
pYPQAT37
Plasmid#223409PurposeT-DNA vector for SpRY-D10A based A-to-G base editing for dicot plants; NA or NG PAM preference; SpRYD10A-ABE was driven by AtUBQ10 and the sgRNA was driven by AtU3; Hygromycin for plants selection.DepositorInsertAtUBQ10-ecTadA8e-SpRY-D10A-AtU3-gRNA scaffold
UseCRISPRTags3x FLAG and NLSExpressionPlantAvailable SinceApril 10, 2025AvailabilityAcademic Institutions and Nonprofits only -
pYPQAT40
Plasmid#223412PurposeT-DNA vector for SpRY-D10A based A-to-G base editing for monocot plants; NA or NG PAM preference; SpRYD10A-ABE was driven by ZmUbi1 and the sgRNA was driven by OsU3; Hygromycin for plants selection.DepositorInsertZmUbi-ecTadA8e-SpRY-D10A-OsU3-gRNA scaffold
UseCRISPRTags3x FLAG and NLSExpressionPlantAvailable SinceApril 10, 2025AvailabilityAcademic Institutions and Nonprofits only -
pSCARS1
Plasmid#174868PurposeContains TEF1p-TEF1in-hGFP-CYCt cassette and wildtype ARS1DepositorInsertARS1
UseSynthetic BiologyExpressionBacterial and YeastAvailable SinceOct. 29, 2021AvailabilityAcademic Institutions and Nonprofits only -
pJH1371
Plasmid#199631PurposePrgef-1 GPI YFP unc-54 3' UTR C.elegans Pan-neural expression of GPI-YFPDepositorInsertGPI
TagsYFPExpressionWormPromoterPregf-1Available SinceMay 7, 2024AvailabilityIndustry, Academic Institutions, and Nonprofits -
pTH1-SaSrtA
Plasmid#64973PurposeExpresses the wild type of Staphylococcus aureus sortase A in E. coliDepositorInsertsortase A
TagsHis6 tag, Mxe GyrA intein, and chitin-binding pro…ExpressionBacterialMutationdeleted amino acids 1-59PromoterT7 promoterAvailable SinceJuly 7, 2015AvailabilityAcademic Institutions and Nonprofits only -
GFPZFN1.4-B2H
Plasmid#26791DepositorInsertGFP-ZFN1
ExpressionMammalianAvailable SinceNov. 29, 2010AvailabilityAcademic Institutions and Nonprofits only -
GFPZFN2-B2H
Plasmid#26792DepositorInsertGFP-ZFN2
ExpressionMammalianAvailable SinceNov. 29, 2010AvailabilityAcademic Institutions and Nonprofits only -
pre_II_CS3_7653_v2
Plasmid#126855Purpose7653 b scaffold from compatibility group 3, made by method II, containing 1 Zn-dependent self-cleaving DNAzyme cassette for linearisation into 7560 b scaffoldDepositorInsertII_CS3_7653_v2
UseSynthetic BiologyAvailable SinceSept. 29, 2020AvailabilityAcademic Institutions and Nonprofits only -
pcDNA3.1 HA-rnSOX9
Plasmid#62972PurposeExpresses HA-tagged rat SOX9DepositorAvailable SinceMay 28, 2015AvailabilityAcademic Institutions and Nonprofits only -
pZY102
Plasmid#73933PurposeThis plasmid was derived from pZY101 by inserting a GUS-intron reporter gene cassette into multiple cloning sites. This plasmid is recommended for use as empty control for service event request.DepositorInsertsbar
GUS
ExpressionPlantMutationintron is inserted into the GUS coding regionPromoter35SAvailable SinceJuly 24, 2018AvailabilityAcademic Institutions and Nonprofits only -
pc3xTC20-alphaTubulin
Plasmid#36327DepositorInsertalpha tubulin 1b with optimized tetracysteine peptides for ReAsH labeling (TUBA1B Synthetic, Human)
Tags3xTC20ExpressionMammalianPromoterCMVAvailable SinceMay 24, 2012AvailabilityAcademic Institutions and Nonprofits only -
PclpB riboswitch GFP pZa
Plasmid#170078PurposeGFP expression under the control of E. coli clpB promoter engineered with riboregulatorsDepositorInsertGreen fluorescent protein
UseSynthetic BiologyExpressionBacterialPromoterPclpBAvailable SinceSept. 10, 2021AvailabilityAcademic Institutions and Nonprofits only -
pFW5000
Plasmid#224214PurposeE. coli-Bacteroides shuttle plasmid; pB8-51 (Bacteroides replication origin), p15A (E. coli replication origin), tetQDepositorTypeEmpty backboneUseSynthetic BiologyExpressionBacterialAvailable SinceSept. 11, 2024AvailabilityAcademic Institutions and Nonprofits only -
2a-copGFP(+HA)
Plasmid#83806PurposeExpress copGFP reporter for HDR-induced knock-inDepositorInsertcopGFP
UseHomologous recombination for gapdh lociAvailable SinceNov. 21, 2016AvailabilityAcademic Institutions and Nonprofits only -
pcDNA3.1-subtilase A
Plasmid#35948DepositorAvailable SinceNov. 14, 2012AvailabilityAcademic Institutions and Nonprofits only -
pMZ3012
Plasmid#204400PurposeA C4-HSL sensor for B. subtilis on a shuttle vector for B. subtilis and E. coli.DepositorInsertsUseSynthetic BiologyPromoterPftsH and Synthetic promoter PRhl0L0L12Available SinceDec. 12, 2023AvailabilityAcademic Institutions and Nonprofits only -
pTNSQCas
Plasmid#219434PurposeCRISPR-associated transposases from V. cholerae and used for RNA guided cargo insertion in Vibrio natriegensDepositorInsertTnsA, TnsB, TnsC, TniQ, Cas8, Cas7, Cas6
ExpressionBacterialAvailable SinceJune 12, 2024AvailabilityAcademic Institutions and Nonprofits only -
pDonor-LoxP
Plasmid#219436PurposeCRISPR-associated transposases from V. cholerae and used for RNA guided cargo insertion in Vibrio natriegensDepositorInserttwo transposon ends, LoxP-terminator-LoxP gene cassette
ExpressionBacterialAvailable SinceJune 4, 2024AvailabilityAcademic Institutions and Nonprofits only -
pGEX-6p-1 Sesn2
Plasmid#61873Purposebacterial expression of a GST-Sesn2 (mouse)DepositorAvailable SinceJan. 20, 2015AvailabilityAcademic Institutions and Nonprofits only -
Ef1a_NANOG_Ires_Puro
Plasmid#18920DepositorAvailable SinceSept. 4, 2008AvailabilityAcademic Institutions and Nonprofits only -
PECAM-H2B-GFP
Plasmid#14689DepositorAvailable SinceMay 15, 2007AvailabilityAcademic Institutions and Nonprofits only -
EF.mHES1.Ubc.GFP
Plasmid#17625DepositorAvailable SinceApril 3, 2008AvailabilityAcademic Institutions and Nonprofits only -
pYPQ-NF-L3-NF-L1-dMb2Cas12a
Plasmid#177686PurposeGateway entry clone (attL1 & attR5) for a single chain FokI-dMb2Cas12a genome editingDepositorInsert2xFokI-dMb2Cas12a
UseCRISPR; Gateway compatible 2xfoki-dmb2cas12a entr…TagsNLSExpressionPlantAvailable SinceApril 19, 2022AvailabilityAcademic Institutions and Nonprofits only -
p3E-U6a-U6c
Plasmid#107599PurposeAn entry vector with U6a and U6c promoter driving guide RNAs expressionDepositorInsertsU6a promoter
U6c promoter
UseCRISPRAvailable SinceJuly 25, 2018AvailabilityAcademic Institutions and Nonprofits only -
pRS406 ATG9 stopGFP
Plasmid#114669PurposeExpresses yeast ATG9 under the endogenous promoter with a stop codon introduced before GFP C-terminal tagDepositorInsertATG9
TagsGFPExpressionYeastMutationChanged the three linker base pairs after the las…PromoterATG9Available SinceSept. 14, 2018AvailabilityAcademic Institutions and Nonprofits only -
pTrex-b-NLS-hSpCas9
Plasmid#62543PurposeExpression of Cas9 in T. cruziDepositorInsertNLS-hSpCas9
UseCRISPR; Trypanosoma cruzi expressionTags3xFLAG and NLSPromoterRibo-HX1Available SinceApril 16, 2015AvailabilityAcademic Institutions and Nonprofits only -
Oct4-ires-EGFP(lox neo)
Plasmid#21547DepositorInsertEGFP, Oct4
UseMouse TargetingAvailable SinceAug. 12, 2009AvailabilityAcademic Institutions and Nonprofits only -
L1-P941L
Plasmid#15498DepositorAvailable SinceOct. 12, 2007AvailabilityAcademic Institutions and Nonprofits only