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pLX304_CMV::mCherry-LgBiT-FLAG-NES

Plasmid

#199715

Purpose

Expresses LgBiT, fused to mCherry, in the cytosol of mammalian cells. Can be used in generating stable cell lines via lentiviral transduction.

Depositor

Alice Ting

Publication

Roh et al J Am Chem Soc. 2023 May 10;145(18):10220

Insert

mCherry-LgBiT-FLAG-NES

Use

Lentiviral and Luciferase

Expression

Mammalian

Promoter

CMV

Available since

May 9, 2023

Availability

Academic Institutions and Nonprofits only

Cited by

1 Article

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pBbS5a-Opto-Cre-Vvd-2

Plasmid

#160400

Purpose

Updated light-inducible Cre recombinase split with Vivid photodimers for bacterial expression.

Depositor

Mary Dunlop

Publication

Sheets et al ACS Synth Biol. 2020 Feb 21;9(2):227-235

Insert

Opto-Cre-Vvd-2

Use

Cre/Lox and Synthetic Biology

Expression

Bacterial

Promoter

lacUV5

Available since

Oct. 20, 2020

Availability

Academic Institutions and Nonprofits only

Cited by

1 Article

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pKS070 - pCAGGS-3XFLAG-(human)CTCF-eGFP

Plasmid

#156448

Purpose

For transient expression of human CTCF tagged with eGFP

Depositor

Elphege Nora

Publication

Nora et al Nat Commun. 2020 Nov 5;11(1):5612. doi:

Insert

human CTCF (CTCF Human)

Expression

Mammalian

Mutation

none

Available since

Sept. 2, 2020

Availability

Academic Institutions and Nonprofits only

Cited by

3 Articles

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pTol2-HuC(elavl3)-CaMPARI2

Plasmid

#137185

Purpose

pan-neuronal expression of CaMPARI2 in zebrafish, used to generate the CaMPARI2 zebrafish line at ZIRC (ZL13801)

Depositor

Eric Schreiter

Publication

Moeyaert et al Nat Commun. 2018 Oct 25;9(1):4440. doi:

Insert

HuC(elavl3)-CaMPARI2-FLAG-HA-myc-polyA

Use

Tol2 plasmid for zebrafish

Tags

FLAG, HA, myc

Promoter

HuC(elavl3)

Available since

March 10, 2020

Availability

Academic Institutions and Nonprofits only

Cited by

3 Articles

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CyanoGate Kit

Plasmid kit

#1000000146

Purpose

Vectors for generating multi-part assemblies in either integrative (suicide) or self-replicating plasmid vectors for working in cyanobacteria.

Depositor

Alistair McCormick

Application

Cloning and Synthetic Biology

Vector type

Bacterial Expression

Cloning type

Golden Gate (MoClo)

Available since

Jan. 14, 2020

Availability

Academic Institutions and Nonprofits only
FRESCA-syntide

Plasmid

#132415

Purpose

FRET-based sensor for CaMKII activity

Depositor

Margaret Stratton

Publication

Ardestani et al J Biol Chem. 2019 Jun 14. pii: RA119.009

Insert

FRESCA-syntide

Expression

Bacterial and Mammalian

Promoter

CMV

Available since

Oct. 29, 2019

Availability

Academic Institutions and Nonprofits only

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HR180-LGR5-GFP

Plasmid

#129092

Purpose

KI EGFP with a puromycin/mRuby selection cassette into human LGR5 locus

Depositor

Toshiro Sato

Publication

Shimokawa et al Nature. 2017 May 11;545(7653):187-192. d

Insert

EGFP and LGR5 homology arms (LGR5 Human)

Use

Homologous recombination donor plasmid

Available since

Sept. 30, 2019

Availability

Academic Institutions and Nonprofits only

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pSGAb-spe

Plasmid

#122000

Purpose

A sgRNA expression plasmid for genome editing in Acinetobacter baumannii

Depositor

Quanjiang Ji

Publication

Wang et al Cell Chem Biol. 2019 Sep 17. pii: S2451-

Insert

none

Use

CRISPR

Available since

Sept. 5, 2019

Availability

Academic Institutions and Nonprofits only

Cited by

1 Article

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pFD152

Plasmid

#125546

Purpose

derivative of pFD116 with an optimized RBS for E. coli

Depositor

David Bikard

Publication

Depardieu et al Methods. 2019 Aug 1. pii: S1046-2023(18)

Insert

optimized RBS

Use

CRISPR

Promoter

anhydrotetracycline Ptet promoter

Available since

Aug. 23, 2019

Availability

Academic Institutions and Nonprofits only

Cited by

5 Articles

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pFRT_TO_FlagHA_G3BP1

Plasmid

#127106

Depositor

Thomas Tuschl

Publication

Meyer et al Mol Cell. 2020 Mar 19;77(6):1193-1205.e5

Insert

G3BP1 (G3BP1 Human)

Expression

Mammalian

Available since

July 31, 2019

Availability

Academic Institutions and Nonprofits only

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pAAV-TRE-fDIO-GFP-IRES-tTA

Plasmid

#118026

Purpose

AAV vector for sparse and bright labeling in a cell-type specific manner

Depositor

Minmin Luo

Publication

Lin et al Nat Methods. 2018 Nov 19. pii: 10.1038/s

Insert

TRE-fDIO-GFP-IRES-tTA

Use

AAV

Mutation

See depositor comments below

Promoter

TRE

Available since

Dec. 4, 2018

Availability

Academic Institutions and Nonprofits only

Cited by

6 Articles

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pSGKP-spe

Plasmid

#117234

Purpose

A sgRNA expression plasmid for genome editing in Klebsiella Pneumoniae

Depositor

Quanjiang Ji

Publication

Wang et al Appl Environ Microbiol. 2018 Sep 14. pii

Type

Empty backbone

Use

CRISPR and Synthetic Biology

Expression

Bacterial

Available since

Nov. 7, 2018

Availability

Academic Institutions and Nonprofits only

Cited by

4 Articles

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pScI_dCas9-CDA-UL

Plasmid

#108551

Purpose

Bacterial Target-AID UGI-LVA vector

Depositor

Akihiko Kondo

Publication

Banno et al Nat Microbiol. 2018 Feb 5. pii: 10.1038/

Insert

dCas9-PmCDA1-UGI-LVA

Tags

Flag

Expression

Bacterial

Mutation

D10A and H840A for SpCas9

Promoter

lambda OR

Available since

Oct. 30, 2018

Availability

Academic Institutions and Nonprofits only

Cited by

1 Article

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p3s-Sniper-Cas9

Plasmid

#113912

Purpose

Expresses Sniper-Cas9 with CMV promoter

Depositor

Jungjoon Lee

Publication

Lee et al Nat Commun. 2018 Aug 6;9(1):3048. doi: 1

Insert

Sniper-Cas9

Use

CRISPR

Expression

Mammalian

Promoter

CMV

Available since

Aug. 8, 2018

Availability

Academic Institutions and Nonprofits only

Cited by

6 Articles

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pHR_PGK_antiHer24D5-8_synNotch_Gal4VP64

Plasmid

#85425

Purpose

Lentiviral vector for expression of anti-Her2 scFv 24D5-8 Gal4VP64 synthetic Notch

Depositor

Wendell Lim

Publication

Roybal et al Cell. 2016 Oct 6;167(2):419-432.e16. doi

Insert

anti-Her2_4D5-8_synNotch

Use

Lentiviral

Promoter

PGK

Available since

July 31, 2018

Availability

Academic Institutions and Nonprofits only

Cited by

1 Article
FKBP-mCherry

Plasmid

#108122

Purpose

Recruitable fluorophore

Depositor

Gerry Hammond

Publication

Zewe et al Elife. 2018 Feb 20;7. pii: 35588. doi: 1

Insert

FKBP1A(isoform a, 3-108):mCherry (FKBP1A Human)

Expression

Mammalian

Promoter

CMV

Available since

April 3, 2018

Availability

Academic Institutions and Nonprofits only

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pDONR223_HSP90B1_WT

Plasmid

#82130

Purpose

Gateway Donor vector containing HSP90B1 , part of the Target Accelerator Plasmid Collection.

Depositor

Jesse Boehm, William Hahn, David Root

Publication

Kim et al Cancer Discov. 2016 Jul;6(7):714-26. doi

Insert

HSP90B1 (HSP90B1 Human)

Use

Gateway Cloning

Mutation

WT

Promoter

None

Available since

April 24, 2017

Availability

Academic Institutions and Nonprofits only

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pY095

Plasmid

#84744

Purpose

Expresses huLbCpf1-T2A-GFP and crRNA guide

Depositor

Feng Zhang

Publication

Zetsche et al Nat Biotechnol. 2017 Jan;35(1):31-34. do

Inserts

huLbCpf1
GFP

Use

CRISPR

Tags

3xHA, NLS, and T2A

Expression

Mammalian

Promoter

CMV

Available since

Jan. 25, 2017

Availability

Academic Institutions and Nonprofits only

Cited by

2 Articles

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Tol2 Gateway compatible toolbox for the study of the nervous system and neurodegenerative disease

Plasmid kit

#1000000087

Purpose

A plasmid collection, based on the Tol2kit, used to streamline the production of transgenic zebrafish for studying neurodegenerative disorders.

Depositor

Nicholas Cole

Application

Cloning and Synthetic Biology

Vector type

Zebrafish Expression

Cloning type

MultiSite Gateway

Available since

Oct. 13, 2016

Availability

Academic Institutions and Nonprofits only
pME-loxP-mTagBFP2-stop-pA-loxP

Plasmid

#75158

Purpose

Floxed TagBFP2 (blue) fluorophore with stop codon for inducible lines.

Depositor

Nicholas Cole

Publication

Don et al Zebrafish. 2016 Sep 15.

Insert

loxP-mTagBFP2-stop-pA-loxP

Use

Middle entry vector for multisite gateway three f…

Promoter

n/a

Available since

Oct. 6, 2016

Availability

Academic Institutions and Nonprofits only

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