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Plasmid#191147PurposeExpression of the sequence matching the 54 extra bases in EK0362 in mammalian cellsDepositorInsertBdfn.t+54
UseTagsExpressionMammalianMutationWTPromoterCMV-TOAvailable sinceOct. 26, 2022AvailabilityAcademic Institutions and Nonprofits only -
EK0341 SFFV-mCherry-SG(Bdnf.s1L153)SG-EGFP (FLP-IN)
Plasmid#191150PurposeExpression of a 153 bp long sensor for murine Bdnf 3' UTR in mammalian cellsDepositorInsertmCherry:Bdnf.s1L153:EGFP
UseTagsExpressionMammalianMutationWTPromoterSFFVAvailable sinceOct. 26, 2022AvailabilityAcademic Institutions and Nonprofits only -
EK0358 SFFV-mCherry-SG(Bdnf.s1L36)SG-EGFP (FLP-IN)
Plasmid#191152PurposeExpression of a 36 bp long sensor for murine Bdnf 3' UTR in mammalian cellsDepositorInsertmCherry:Bdnf.s1L36:EGFP
UseTagsExpressionMammalianMutationWTPromoterSFFVAvailable sinceOct. 26, 2022AvailabilityAcademic Institutions and Nonprofits only -
pIALD2E
Plasmid#185866PurposeKnocking out ALD6DepositorInsertALD(-125, 40)- ALD6(1054, 1749)
UseTagsExpressionYeastMutationNonePromoterAvailable sinceSept. 6, 2022AvailabilityAcademic Institutions and Nonprofits only -
EGFP-Myo1b motor
Plasmid#187364PurposeExpresses rat myosin 1b isoform b motor domain labelled with EGFPDepositorInsertmyosin 1b isoform b motor domain (Myo1b Rat)
UseTagsEGFPExpressionMammalianMutationPromoterCMVAvailable sinceAug. 22, 2022AvailabilityAcademic Institutions and Nonprofits only -
pX601‐mhCMV‐miniABEmax‐N3‐ E53ogRNA
Plasmid#187064PurposeNpu Split N-terminal half of ABEmaxNGA with gRNA targeting mdx4cv nonsense mutationDepositorInsertminiABEmax-Npu
UseAAV and CRISPRTagsExpressionMammalianMutationPromotermhCMVAvailable sinceAug. 8, 2022AvailabilityAcademic Institutions and Nonprofits only -
EGFP-Myo1b-Tail
Plasmid#187365PurposeExpresses rat myosin 1b isoform b Tail domain labelled with EGFPDepositorInsertmyosin 1b isoform b Tail domain (Myo1b Rat)
UseTagsEGFPExpressionMammalianMutationPromoterCMVAvailable sinceAug. 5, 2022AvailabilityAcademic Institutions and Nonprofits only -
pLenti_CMV:Flag-CeNL_SV40:PuroR
Plasmid#183039PurposeExpresses the FLAG-tagged CeNL (Blue Nanolantern) in mammalian cells.DepositorInsertCeNL Nanolantern
UseLentiviralTagsFLAGExpressionMammalianMutationPromoterCMVAvailable sinceJuly 11, 2022AvailabilityAcademic Institutions and Nonprofits only -
pLenti_CMV:Flag-YeNL_SV40:PuroR
Plasmid#183041PurposeExpresses the FLAG-tagged YeNL (Yellow Nanolantern) in mammalian cells.DepositorInsertYeNL Nanolantern
UseLentiviralTagsFLAGExpressionMammalianMutationPromoterCMVAvailable sinceJuly 11, 2022AvailabilityAcademic Institutions and Nonprofits only -
pLenti_CMV:Flag-OeNL_SV40:PuroR
Plasmid#183042PurposeExpresses the FLAG-tagged OeNL (Orange Nanolantern) in mammalian cells.DepositorInsertOeNL Nanolantern
UseLentiviralTagsFLAGExpressionMammalianMutationPromoterCMVAvailable sinceJuly 11, 2022AvailabilityAcademic Institutions and Nonprofits only -
1056D
Plasmid#183132PurposePlasmid supports expression of 2 gRNAs targeting D.suzukii bTub, Opie-mVenus tagged, and can be integrated with pBac.DepositorInsertU6.3-gRNAs[bTub]
UseCRISPRTagsExpressionInsectMutationPromoterAvailable sinceJune 10, 2022AvailabilityAcademic Institutions and Nonprofits only -
1056E
Plasmid#183133PurposePlasmid supports expression of 2 gRNAs targeting D.suzukii dsx, Opie-mVenus tagged, and can be integrated with pBac.DepositorInsertU6.3-gRNAs[dsx]
UseCRISPRTagsExpressionInsectMutationPromoterAvailable sinceJune 10, 2022AvailabilityAcademic Institutions and Nonprofits only -
1056F
Plasmid#183134PurposePlasmid supports expression of 2 gRNAs targeting D.suzukii tra, Opie-mVenus tagged, and can be integrated with pBac.DepositorInsertU6.3-gRNAs[tra]
UseCRISPRTagsExpressionInsectMutationPromoterAvailable sinceJune 10, 2022AvailabilityAcademic Institutions and Nonprofits only -
pYPQ-NF1-L1-dSpRY
Plasmid#177681PurposeGateway entry clone (attL1 & attR5) for dimeric FokI-dCas9-SpRY genome editingDepositorInsertFokI-dCas9-SpRY
UseCRISPR; Gateway compatible foki-dcas9-spry entry …TagsNLSExpressionPlantMutationPromoterAvailable sinceApril 19, 2022AvailabilityAcademic Institutions and Nonprofits only -
pYPQ-NF1-L2-dSpRY
Plasmid#177682PurposeGateway entry clone (attL1 & attR5) for dimeric FokI-dCas9-SpRY genome editingDepositorInsertFokI-dCas9-SpRY
UseCRISPR; Gateway compatible foki-dcas9-spry entry …TagsNLSExpressionPlantMutationPromoterAvailable sinceApril 19, 2022AvailabilityAcademic Institutions and Nonprofits only -
pYPQ-NF-L2-dMb2Cas12a
Plasmid#177684PurposeGateway entry clone (attL1 & attR5) for dimeric FokI-dMb2Cas12a genome editingDepositorInsertFokI-dMb2Cas12a
UseCRISPR; Gateway compatible foki-dmb2cas12a entry …TagsNLSExpressionPlantMutationPromoterAvailable sinceApril 19, 2022AvailabilityAcademic Institutions and Nonprofits only -
pBM13g-sd4U
Plasmid#179689PurposeBacterial expression of base editorDepositorInsert[sd4U] UGI-TALE3-DddA-G1397-N (S1330I + A1341V + N1342S + E1370K + T1380I)
UseTagsExpressionBacterialMutationS1330I A1341V N1342S E1370K T1380IPromoterAvailable sinceApril 4, 2022AvailabilityAcademic Institutions and Nonprofits only -
PB-ReNL
Plasmid#123138PurposePiggybac transposon plasmid for live animal optical imaging of Red-enhanced nanolanternDepositorInsertRed-enhanced Nanolantern
UseTagsExpressionMammalianMutationPromoterCAGAvailable sinceFeb. 14, 2022AvailabilityAcademic Institutions and Nonprofits only -
Lentiguide-Blast-eNMU710
Plasmid#172658PurposeExpressing paired pegRNAs from human U6 and H1 promoters to make 710-bp deletion on e-NMU locusDepositorInsertpegRNA-eNMUA/pegRNA-eNMUB
UseCRISPRTagsExpressionMutationPromoterAvailable sinceDec. 7, 2021AvailabilityAcademic Institutions and Nonprofits only -
PB-HD252
Plasmid#172656PurposeExpressing paired pegRNAs from human U6 and H1 promoters to make 252-bp deletion on HPRT1 geneDepositorInsertpegRNA-HD252A/pegRNA-HD252B
UseCRISPRTagsExpressionMutationPromoterAvailable sinceOct. 22, 2021AvailabilityAcademic Institutions and Nonprofits only -
pPRIChp-hOtub1
Plasmid#174743PurposeGene overexpressionDepositorInsertOTUB1 (OTUB1 Human)
UseRetroviralTagsHAExpressionMutationPromoterAvailable sinceSept. 27, 2021AvailabilityAcademic Institutions and Nonprofits only -
pJIT163-AFID-2
Plasmid#167358PurposeFor inducing predictable deletions in protoplasts and wheat plantsDepositorInsertA3A-Cas9-UDG
UseTagsExpressionPlantMutationWTPromoterAvailable sinceMarch 31, 2021AvailabilityAcademic Institutions and Nonprofits only -
IBMc120
Plasmid#161950PurposeContains elements for building reporter plasmid for IBM. Built upon pSB4A3. ECF20 reporter plasmidDepositorInsertpSB4A3-PECF20-B30-mScarlet-I-B15
UseTagsExpressionBacterialMutationPromoterPECF20Available sinceMarch 23, 2021AvailabilityAcademic Institutions and Nonprofits only -
IBMc393
Plasmid#161953PurposeDemonstrates chemically induced dimmerization of acVHH. T7 RNAP polymerase reporter plasmidDepositorInsertpSB4A3-T-PT7-B30-mScarlet-I-3T
UseTagsExpressionBacterialMutationPromoterPT7Available sinceMarch 16, 2021AvailabilityAcademic Institutions and Nonprofits only -
IBMc330
Plasmid#161952PurposeContains elements for building reporter plasmid for IBM. SrpR reporter plasmidDepositorInsertpSEVA171-PSrpR-B30-mScarlet-I-Ter
UseTagsExpressionBacterialMutationPromoterPSrpRAvailable sinceMarch 14, 2021AvailabilityAcademic Institutions and Nonprofits only -
IBMc050
Plasmid#161938PurposeCarries Golden Gate substitution insert for IBM with M86 intein. Plux2 as C-lobe promoterDepositorInsertpSB1A3-BbsI-M86N-cat-Plux2-B32-M86C-SapI
UseTagsExpressionBacterialMutationPromoterPlux2Available sinceMarch 14, 2021AvailabilityAcademic Institutions and Nonprofits only -
IBMc259
Plasmid#161951PurposeContains elements for building reporter plasmid for IBM.TetR reporter plasmidDepositorInsertpSEVA471-PTet*-RiboJ10-B64-mScarlet-I-Ter
UseTagsExpressionBacterialMutationPromoterPTet*Available sinceMarch 14, 2021AvailabilityAcademic Institutions and Nonprofits only -
VanR - F165V
Plasmid#158965PurposeWild type VanR where the residue in position 165 was mutated from Phenylalanine to Valine. This mutation abolished the response to vanillic acid. The gene is under the control of the TEF1 promoter.DepositorInsertVanR - F165V
UseTagsExpressionYeastMutationWild type VanR from Caulobacter crescentus with m…PromoterTEF1Available sinceJan. 8, 2021AvailabilityAcademic Institutions and Nonprofits only -
VanR - F165R
Plasmid#158966PurposeWild type VanR where the residue in position 165 was mutated from Phenylalanine to Arginine. This mutation abolished the response to vanillic acid. The gene is under the control of the TEF1 promoter.DepositorInsertVanR - F165R
UseTagsExpressionYeastMutationWild type VanR from Caulobacter crescentus with m…PromoterTEF1Available sinceJan. 8, 2021AvailabilityAcademic Institutions and Nonprofits only -
pMIII-Neo-AM
Plasmid#153427PurposeRetroviral (MSCV) expression of ametrine fluorescent proteinDepositorInsertametrine
UseRetroviralTagsExpressionMammalianMutationPromoterAvailable sinceAug. 11, 2020AvailabilityAcademic Institutions and Nonprofits only