We narrowed to 50,385 results for: Son

1. tdTomato-H2B-6

   Plasmid

   #58101

   Purpose

   Localization: Nucleus/Histones, Excitation: 554, Emission: 581

   Depositor

   Michael Davidson

   Article

   Planchon et al Nat Methods. 2011 May;8(5):417-23. doi:

   Insert

   H2B (H2BC11 Human)
   

   Use

   Tags

   tdTomato

   Expression

   Mammalian

   Mutation

   D26G and V119I in H2B

   Promoter

   CMV

   Available since

   Dec. 23, 2014

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

2. pLKO.1-puro_CMV_NES-Caprola_06-mEGFP

   Plasmid

   #194693

   Purpose

   CMV driven expression of the calcium recorder Caprola_06 fused to mEGFP for neuronal expression through through lentivirus transduction

   Depositor

   Kai Johnsson

   Article

   Huppertz et al Science. 2024 Feb 23;383(6685):890-897.

   Insert

   Caprola_06-mEGFP
   

   Use

   Lentiviral

   Tags

   mEGFP

   Expression

   Mutation

   Promoter

   CMV

   Available since

   March 13, 2024

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

3. mTagBFP2-Farnesyl-5

   Plasmid

   #55295

   Purpose

   Localization: Plasma Membrane, Excitation: 399, Emission: 456

   Depositor

   Michael Davidson

   Article

   Subach et al PLoS One. 2011;6(12):e28674. Epub 2011 D

   Insert

   Farnesyl (HRAS Human)
   

   Use

   Tags

   mTagBFP2

   Expression

   Mammalian

   Mutation

   encodes final 20 aa of NM_001130442.1

   Promoter

   CMV

   Available since

   Oct. 10, 2014

   Availability

   Industry, Academic Institutions, and Nonprofits

   

   Enlarge

4. pLKO.1-puro_CMV_NES-Caprola_off-mEGFP

   Plasmid

   #194695

   Purpose

   CMV driven expression of the inactive calcium recorder negative control Caprola_off fused to mEGFP for neuronal expression through lentivirus transduction

   Depositor

   Kai Johnsson

   Article

   Huppertz et al Science. 2024 Feb 23;383(6685):890-897.

   Insert

   Caprola_off-mEGFP
   

   Use

   Lentiviral

   Tags

   mEGFP

   Expression

   Mutation

   Promoter

   CMV

   Available since

   Feb. 23, 2024

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

5. pCAGGS_3E5E_eGFP

   Plasmid

   #103054

   Purpose

   Encodes Ebola virus minigenome with EGFP reporter gene. Minigenome is under control of the CAG promoter

   Depositor

   Elke Mühlberger

   Article

   Nelson et al Antiviral Res. 2017 Aug 12;146:21-27. do

   Insert

   Ebola virus (ebolavirus Zaire, Mayinga) minigenome
   

   Use

   Tags

   3E5E eGFP cloned into pCAGGS vector from pCDNA3.1…

   Expression

   Mammalian

   Mutation

   Promoter

   CAG

   Available since

   Dec. 21, 2017

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

6. EGFP-Actin-7

   Plasmid

   #56421

   Purpose

   Localization: Actin, Excitation: 488, Emission: 507

   Depositor

   Michael Davidson

   Article

   Rizzo et al Cold Spring Harb Protoc. 2009 Dec;2009(1

   Insert

   Actin (ACTB Human)
   

   Use

   Tags

   EGFP

   Expression

   Mammalian

   Mutation

   Promoter

   CMV

   Available since

   Dec. 23, 2014

   Availability

   Industry, Academic Institutions, and Nonprofits

   

   Enlarge

7. pcDNA5/FRT/TO_TOMM20-SNAP-tag2-T2A-EGFP

   Plasmid

   #226516

   Purpose

   Mamalian cell expression of SNAP-tag2 on mitochondrial surface and cytosolic EGFP

   Depositor

   Kai Johnsson

   Article

   Kühn et al Nat Chem Biol. 2025 Jul 3. doi: 10.1038/

   Insert

   TOMM20-SNAP-tag2-T2A-EGFP
   

   Use

   Tags

   Expression

   Mammalian

   Mutation

   Promoter

   CMV

   Available since

   Oct. 15, 2024

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

8. pCDNA5/FRT_CMV_NES-Caprola_05-mEGFP

   Plasmid

   #194683

   Purpose

   CMV driven expression of the calcium recorder Caprola_05 in mammalian cell lines fused to mEGFP

   Depositor

   Kai Johnsson

   Article

   Huppertz et al Science. 2024 Feb 23;383(6685):890-897.

   Insert

   Caprola_05-mEGFP
   

   Use

   Tags

   mEGFP

   Expression

   Mammalian

   Mutation

   Promoter

   CMV

   Available since

   Feb. 23, 2024

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

9. pAAV_CAG_Kinprola_PKA-mEGFP-NLS3__WPRE-SV40

   Plasmid

   #233365

   Purpose

   CAG driven expression of the PKA activity recorder Kinprola_PKA fused to mEGFP for mammalian cell expression through AAV transduction

   Depositor

   Kai Johnsson

   Article

   Sun et al Nat Chem Biol. 2025 Jul 10. doi: 10.1038

   Insert

   Kinprola_PKA-mEGFP-NLS3X
   

   Use

   AAV

   Tags

   mEGFP-NLS3X

   Expression

   Mutation

   Promoter

   CAG

   Available since

   April 14, 2025

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

10. pAAV_CAG_NES-Kinprola_PKA-mTagBFP2_WPRE-SV40

    Plasmid

    #233364

    Purpose

    CAG driven expression of the PKA activity recorder Kinprola_PKA fused to mTagBFP2 for mammalian cell expression through AAV transduction

    Depositor

    Kai Johnsson

    Article

    Sun et al Nat Chem Biol. 2025 Jul 10. doi: 10.1038

    Insert

    NES-Kinprola_PKA-mTagBFP2
    

    Use

    AAV

    Tags

    NES and mTagBFP2

    Expression

    Mutation

    Promoter

    CAG

    Available since

    March 26, 2025

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

11. pCDNA5/FRT/TO_TOMM20_HaloTag9_T2A_EGFP

    Plasmid

    #169333

    Purpose

    Expression of HaloTag9 at the mitochondrial surface of mammalian cells

    Depositor

    Kai Johnsson

    Article

    Frei et al Nat Methods. 2021 Dec 16. pii: 10.1038/s

    Insert

    TOMM20-HaloTag9 (TOMM20 Human)
    

    Use

    Tags

    HaloTag9

    Expression

    Mammalian

    Mutation

    HaloTag9 = HaloTag7-Q165H-P174R

    Promoter

    Available since

    Nov. 9, 2021

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

12. pcDNA5/FRT/TO_Cox8a(4x)-SNAP-tag2-T2A-EGFP

    Plasmid

    #226515

    Purpose

    Mamalian cell expression of SNAP-tag2 in the mitochondrial matrix and cytosolic EGFP

    Depositor

    Kai Johnsson

    Article

    Kühn et al Nat Chem Biol. 2025 Jul 3. doi: 10.1038/

    Insert

    Cox8a(4x)-SNAP-tag2-T2A-EGFP
    

    Use

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    CMV

    Available since

    Oct. 15, 2024

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

13. AAV-Cre-GFP

    Plasmid

    #68544

    Purpose

    AAV expression of Cre-GFP fusion

    Depositor

    Eric Nestler

    Article

    Kennedy et al Nat Neurosci. 2013 Apr;16(4):434-40. doi

    Insert

    Cre
    

    Use

    AAV and Cre/Lox

    Tags

    EGFP

    Expression

    Mammalian

    Mutation

    Promoter

    CMV

    Available since

    Sept. 3, 2015

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

14. pCDNA5/FRT_CMV_Kinprola_PKA-mEGFP-MTBD

    Plasmid

    #233363

    Purpose

    CMV driven expression of the PKA activity recorder Kinprola_PKA in mammalian cell lines fused to mEGFP, targeting to the microtubule

    Depositor

    Kai Johnsson

    Article

    Sun et al Nat Chem Biol. 2025 Jul 10. doi: 10.1038

    Insert

    Kinprola_PKA-mEGFP-MTBD
    

    Use

    Tags

    mEGFP-MTBD

    Expression

    Mammalian

    Mutation

    Promoter

    CMV

    Available since

    March 26, 2025

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

15. pCI-Neo-PTPRT (WT)

    Plasmid

    #16630

    Depositor

    Bert Vogelstein

    Article

    Wang et al Science. 2004 May 21. 304(5674):1164-6.

    Insert

    PTPRT (PTPRT Human)
    

    Use

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    Available since

    March 12, 2008

    Availability

    Academic Institutions and Nonprofits only

16. pEBB HA RelA

    Plasmid

    #74892

    Purpose

    Express RelA in mammalian cells

    Depositor

    Colin Duckett, Ezra Burstein

    Article

    Burstein et al J Biol Chem. 2005 Jun 10;280(23):22222-3

    Insert

    RelA (RELA Human)
    

    Use

    Tags

    HA

    Expression

    Mammalian

    Mutation

    Promoter

    EF-1alpha promoter

    Available since

    June 23, 2016

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

17. EF-hGPC3

    Plasmid

    #24670

    Depositor

    Jorge Filmus

    Article

    Gonzalez et al J Cell Biol. 1998 Jun 15. 141(6):1407-14

    Insert

    GPC3 (GPC3 Human)
    

    Use

    Tags

    HA

    Expression

    Mammalian

    Mutation

    Promoter

    Available since

    May 6, 2010

    Availability

    Academic Institutions and Nonprofits only

18. pET51b-His-TEV-SNAP-tag_fast

    Plasmid

    #167271

    Purpose

    Overexpression of the fast variant (E30R) of SNAP-tag in E. coli

    Depositor

    Kai Johnsson

    Article

    Wilhelm et al Biochemistry. 2021 Aug 24;60(33):2560-25

    Insert

    SNAPf-tag
    

    Use

    Tags

    His x10

    Expression

    Bacterial

    Mutation

    E30R

    Promoter

    T7

    Available since

    April 14, 2021

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

19. 2XMyc-LRRK2-3XKD

    Plasmid

    #25366

    Depositor

    Mark Cookson

    Article

    J Biol Chem. 2008 Jun 13;283(24):16906-1

    Insert

    LRRK2 (LRRK2 Human)
    

    Use

    Tags

    2XMyc

    Expression

    Mammalian

    Mutation

    "Kinase Dead" Lysine 1906 mutated to A…

    Promoter

    Available since

    June 29, 2010

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

20. pPB-cT3G-cERP2-PRDM1

    Plasmid

    #222924

    Purpose

    PiggyBac transposon plasmid for doxycycline inducible expression of PRDM1

    Depositor

    George Church

    Article

    Pierson Smela et al bioRxiv 2022.07.11.499564

    Insert

    PRDM1 (PRDM1 Human)
    

    Use

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    Available since

    July 22, 2024

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

21. pLenti-V5-KDM3A

    Plasmid

    #82331

    Purpose

    lentiviral expression of KDM3A

    Depositor

    Luke Gaughan

    Article

    Wade et al Nucleic Acids Res. 2015 Jan;43(1):196-20

    Insert

    KDM3A (KDM3A Human)
    

    Use

    Lentiviral

    Tags

    V5

    Expression

    Mammalian

    Mutation

    two silent mutations to make it siRNA resistant

    Promoter

    Available since

    March 14, 2017

    Availability

    Academic Institutions and Nonprofits only

22. pCDNA5/FRT/TO_CEP41_HaloTag9_T2A_EGFP

    Plasmid

    #169328

    Purpose

    Expression of HaloTag9 located at the microtubules of mammalian cells

    Depositor

    Kai Johnsson

    Article

    Frei et al Nat Methods. 2021 Dec 16. pii: 10.1038/s

    Insert

    CEP41-HaloTag9 (CEP41 Human)
    

    Use

    Tags

    HaloTag9

    Expression

    Mammalian

    Mutation

    HaloTag9 = HaloTag7-Q165H-P174R

    Promoter

    Available since

    Nov. 9, 2021

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

23. pLPT234

    Plasmid

    #127855

    Purpose

    Repressilator 2.0, without degradation tags, integrated triple reporter

    Depositor

    Johan Paulsson

    Article

    Riglar et al Nat Commun. 2019 Oct 11;10(1):4665. doi:

    Inserts

    bla
    mSCFP3
    mKate2
    mVenus
    TetR
    LacI
    cI
    

    Use

    Synthetic Biology

    Tags

    Expression

    Bacterial

    Mutation

    First 11aa derived from mCherry as fusion protein

    Promoter

    pAmpR, pL LacO1, pL tetO1, and pR

    Available since

    July 9, 2020

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

24. pEF1a-hMLH1NTD-NLS

    Plasmid

    #174826

    Purpose

    Mammalian expression of human MLH1NTD-NLS (codon optimized)

    Depositor

    David Liu

    Article

    Chen et al Cell. 2021 Oct 28;184(22):5635-5652.e29.

    Insert

    human MLH1_NTD-NLS (codon optimized)
    

    Use

    Tags

    SV40 bpNLS

    Expression

    Mammalian

    Mutation

    truncation of residues 1-335

    Promoter

    EF1a

    Available since

    Oct. 14, 2021

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

25. pDONR223-EGFP

    Plasmid

    #209057

    Purpose

    Gateway-compatible entry vector, with insert of the EGFP gene

    Depositor

    Thomas Gonatopoulos-Pournatzis

    Article

    Xiao et al Mol Cell. 2024 Jul 11;84(13):2553-2572.e

    Insert

    EGFP
    

    Use

    Gateway entry vector

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    Available since

    June 20, 2024

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

26. tdTomato-Gamma-Tubulin-17

    Plasmid

    #58099

    Purpose

    Localization: Centrosomes, Excitation: 554, Emission: 581

    Depositor

    Michael Davidson

    Article

    Shaner et al Nat Methods. 2008 Jun;5(6):545-51. doi:

    Insert

    Gamma-Tubulin (TUBG1 Human)
    

    Use

    Tags

    tdTomato

    Expression

    Mammalian

    Mutation

    Promoter

    CMV

    Available since

    Dec. 19, 2014

    Availability

    Academic Institutions and Nonprofits only

27. pEJS1084_pTetR-P2A-BFP/BspMI flexible sgRNA

    Plasmid

    #117687

    Purpose

    U6 driven Spy sgRNA cloning vector where guide sequences are inserted between BspMI sites. This plasmid works with Addgene #108570 for subnuclear proteomic profiling via C-BERST method.

    Depositor

    Erik Sontheimer

    Article

    Gao et al Methods Enzymol. 2019;616:365-383. doi:

    Inserts

    Kanamycin cassette
    TetR-P2A-BFP
    

    Use

    CRISPR and Lentiviral

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    U6 and hPGK

    Available since

    Dec. 17, 2018

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

28. pcDNA3.1-CHRM3-cpHalo∆-mEGFP

    Plasmid

    #194678

    Purpose

    CMV driven expression of the m3 GPCR recorder in mammalian cell lines fused to mEGFP

    Depositor

    Kai Johnsson

    Article

    Huppertz et al Science. 2024 Feb 23;383(6685):890-897.

    Insert

    CHRM3_cpHalo∆
    

    Use

    Tags

    mEGFP

    Expression

    Mammalian

    Mutation

    Promoter

    CMV

    Available since

    Feb. 23, 2024

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

29. pRDA_355

    Plasmid

    #187159

    Purpose

    Lentiviral expression of doxycycline inducible customizable Sp Cas9 sgRNA

    Depositor

    David Root, John Doench

    Article

    Aquilanti et al Neuro Oncol. 2023 Jul 6;25(7):1275-1285.

    Type

    Empty backbone

    Use

    CRISPR and Lentiviral

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    Available since

    July 8, 2022

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

30. retro-gRNA-mRFP1

    Plasmid

    #112914

    Purpose

    Retrovirus expressing mRFP1 with BBSI cloning sites for sgRNA

    Depositor

    Christophe Benoist, Diane Mathis

    Article

    Magnuson et al Proc Natl Acad Sci U S A. 2018 Nov 6;115

    Insert

    mRFP1
    

    Use

    Retroviral

    Tags

    Expression

    Mutation

    Promoter

    EF1a

    Available since

    Oct. 23, 2018

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

31. TPST2-EGFP

    Plasmid

    #66618

    Purpose

    Encodes GFP fusion of TPST2 trans Golgi marker (Tyrosyl protein sulfotransferase 2)

    Depositor

    David Stephens

    Article

    Spooner et al Biochem J. 2008 Sep 15;414(3):471-84. do

    Insert

    TPST2 (TPST2 Human)
    

    Use

    Tags

    Expression

    Mammalian

    Mutation

    Insert contains T370A relative to NM_001008566.1

    Promoter

    Available since

    July 28, 2015

    Availability

    Academic Institutions and Nonprofits only

32. pETSUMO2_Vitiosangium-bGSDM-thrombin

    Plasmid

    #222099

    Purpose

    For expression of a mutant bacterial gasdermin (bGSDM) encoded by a Vitiosangium species with a thrombin protease cleavage site for controlled activation.

    Depositor

    Philip Kranzusch

    Article

    Johnson et al Nature. 2024 Apr;628(8008):657-663. doi:

    Insert

    Vitiosangium-bGSDM-thrombin ORF
    

    Use

    Tags

    6xHis-SUMO2

    Expression

    Bacterial

    Mutation

    N-terminal methionine deleted and replaced by sin…

    Promoter

    T7

    Available since

    July 12, 2024

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

33. pETSUMO2_Nostoc-bGSDM-WELQ

    Plasmid

    #222100

    Purpose

    For expression of a mutant bacterial gasdermin (bGSDM) encoded by a Nostoc species with a WELQut protease cleavage site for controlled activation.

    Depositor

    Philip Kranzusch

    Article

    Johnson et al Nature. 2024 Apr;628(8008):657-663. doi:

    Insert

    Nostoc-bGSDM-WELQ ORF
    

    Use

    Tags

    6xHis-SUMO2

    Expression

    Bacterial

    Mutation

    N-terminal methionine deleted and replaced by sin…

    Promoter

    T7

    Available since

    July 12, 2024

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

34. pcDNA5-Flag-Adrm1

    Plasmid

    #19417

    Depositor

    Joan Conaway, Ronald Conaway

    Article

    Yao et al Nat Cell Biol. 2006 Sep . 8(9):994-1002.

    Insert

    adhesion regulating molecule 1 (ADRM1 Human)
    

    Use

    Tags

    FLAG

    Expression

    Mammalian

    Mutation

    Promoter

    Available since

    Nov. 21, 2008

    Availability

    Academic Institutions and Nonprofits only

35. pZCS36 (Phsp16.41::Cas9(dpiRNA)::tbb-2 ‘3UTR)

    Plasmid

    #193048

    Purpose

    Heatshock expressed Cas9

    Depositor

    Patrick Phillips

    Article

    Stevenson et al Elife. 2023 Jul 4;12:RP84831. doi: 10.75

    Insert

    hsp16.41p::Cas9(dpiRNA)::tbb-2 ‘3UTR
    

    Use

    CRISPR

    Tags

    Expression

    Worm

    Mutation

    Promoter

    hsp16.41p

    Available since

    Sept. 28, 2023

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

36. mTagRFP-T-LaminB1-10

    Plasmid

    #58020

    Purpose

    Localization: Nuclear Envelope, Excitation: 555, Emission: 584

    Depositor

    Michael Davidson

    Article

    Shaner et al Nat Methods. 2008 Jun;5(6):545-51. doi:

    Insert

    LaminB1 (LMNB1 Human)
    

    Use

    Tags

    mTagRFP

    Expression

    Mammalian

    Mutation

    Promoter

    CMV

    Available since

    Nov. 4, 2014

    Availability

    Industry, Academic Institutions, and Nonprofits

    

    Enlarge

37. pYO1273

    Plasmid

    #235746

    Purpose

    Expression of FL human EGFP-ATG14L in mammalian cells

    Depositor

    Roger Williams

    Article

    Ohashi et al Elife. 2020 Jun 30;9:e58281. doi: 10.755

    Insert

    ATG14L (ATG14 Human)
    

    Use

    Tags

    EGFP

    Expression

    Mammalian

    Mutation

    Promoter

    Available since

    May 13, 2025

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

38. pCDNA5/FRT_CMV_NES-Caprola_09-mEGFP

    Plasmid

    #194685

    Purpose

    CMV driven expression of the calcium recorder Caprola_09 in mammalian cell lines fused to mEGFP

    Depositor

    Kai Johnsson

    Article

    Huppertz et al Science. 2024 Feb 23;383(6685):890-897.

    Insert

    Caprola_09-mEGFP
    

    Use

    Tags

    mEGFP

    Expression

    Mammalian

    Mutation

    Promoter

    CMV

    Available since

    Feb. 23, 2024

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

39. pNUT N6His hTFNG

    Plasmid

    #67240

    Purpose

    Expresses N-His tagged nonglycosylated human serum transferrin in mammalian cells

    Depositor

    Ross MacGillivray, Anne Mason

    Article

    Mason et al Biochemistry. 2002 Jul 30;41(30):9448-54

    Insert

    human serum transferrin (TF Human)
    

    Use

    Tags

    N-terminal signal peptide, 4 aa link, 6 His, Fact…

    Expression

    Mammalian

    Mutation

    Asn413 Asp, Asn611Asp

    Promoter

    SV40

    Available since

    Sept. 18, 2015

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

40. pCDNA5/FRT_CMV_NES-Caprola_off-mEGFP

    Plasmid

    #194687

    Purpose

    CMV driven expression of the inactive calcium recorder negative control Caprola_off in mammalian cell lines fused to mEGFP

    Depositor

    Kai Johnsson

    Article

    Huppertz et al Science. 2024 Feb 23;383(6685):890-897.

    Insert

    Caprola_off-mEGFP
    

    Use

    Tags

    mEGFP

    Expression

    Mammalian

    Mutation

    Promoter

    CMV

    Available since

    Feb. 23, 2024

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge