We narrowed to 11,196 results for: ENA

1. pJG370

   Plasmid

   #91187

   Purpose

   BeYDV replicon T-DNA for gene targeting in tobacco leaves, D10A single nickase no gRNA control (nAtCas9_D10A+gRNActrl+donor)

   Depositor

   Daniel Voytas

   Article

   Cermak et al Plant Cell. 2017 May 18. pii: tpc.00922.

   Insert

   nAtCas9_D10A+gRNActrl+donor
   

   Use

   CRISPR

   Tags

   Expression

   Plant

   Mutation

   Promoter

   Available since

   Sept. 29, 2017

   Availability

   Academic Institutions and Nonprofits only

   

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2. pQCi-hTRAC-sgRNA

   Plasmid

   #154093

   Purpose

   pQCi backbone with sgRNA targeting human TCR-alpha common chain

   Depositor

   Scott McComb

   Article

   Bloemberg et al CRISPR J. 2021 Feb;4(1):104-119. doi: 10

   Insert

   sgRNA targeting human TRAC locus
   

   Use

   CRISPR

   Tags

   Expression

   Mammalian

   Mutation

   Promoter

   Available since

   July 28, 2020

   Availability

   Academic Institutions and Nonprofits only

   

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3. G1397 DddAtox-C–dSpCas9

   Plasmid

   #157836

   Purpose

   expresses split DddAtox-Cas9 construct in mammalian cells

   Depositor

   David Liu

   Article

   Mok et al Nature. 2020 Jul 8. pii: 10.1038/s41586-

   Insert

   bpNLS–G1397 DddAtox-C–dSpCas9–UGI–UGI–bpNLS
   

   Use

   CRISPR

   Tags

   Expression

   Mutation

   Promoter

   Available since

   Aug. 4, 2020

   Availability

   Academic Institutions and Nonprofits only

   

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4. AA015

   Plasmid

   #216058

   Purpose

   Fragmid fragment: (2A Selection) Puromycin resistance, T2A, EGFP

   Depositor

   John Doench

   Article

   McGee et al Cell Genom. 2024 Mar 13;4(3):100519. doi

   Has Service

   Cloning Grade DNA

   Insert

   P2A_v1.1; PuroR_v1.1; T2A_v1.2; EGFP_v1.1; Stop
   

   Use

   Fragment

   Tags

   Expression

   Mutation

   Promoter

   Available since

   April 29, 2024

   Availability

   Academic Institutions and Nonprofits only

   

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5. AA176

   Plasmid

   #216063

   Purpose

   Fragmid fragment: (2A Selection) mCherry

   Depositor

   John Doench

   Article

   McGee et al Cell Genom. 2024 Mar 13;4(3):100519. doi

   Has Service

   Cloning Grade DNA

   Insert

   P2A_v1.1; mCherry_v1.1; Stop
   

   Use

   Fragment

   Tags

   Expression

   Mutation

   Promoter

   Available since

   April 29, 2024

   Availability

   Academic Institutions and Nonprofits only

   

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6. p2CT-His-MBP-Lbu_C2c2_R1048A_H1053A

   Plasmid

   #83484

   Purpose

   Bacterial expression of L. buccalis C2c2 CRISPR effector (HEPN nuclease 2 inactive mutant).

   Depositor

   Jennifer Doudna

   Article

   East-Seletsky et al Nature. 2016 Sep 26. doi: 10.1038/nature

   Insert

   Lbu_C2c2_R1048A_H1053A
   

   Use

   CRISPR

   Tags

   His6-MBP

   Expression

   Bacterial

   Mutation

   R1048A, H1053A

   Promoter

   Available since

   Oct. 31, 2016

   Availability

   Academic Institutions and Nonprofits only

   

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7. pMXS-IRES-Blast SHMT1

   Plasmid

   #106304

   Purpose

   Express SHMT1

   Depositor

   Richard Possemato

   Article

   Minton et al Mol Cell. 2018 Feb 15;69(4):610-621.e5.

   Insert

   serine hydroxymethyltransferase 1 (SHMT1 Human)
   

   Use

   Retroviral

   Tags

   Expression

   Mammalian

   Mutation

   Promoter

   Available since

   Dec. 6, 2018

   Availability

   Academic Institutions and Nonprofits only

   

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8. pRVdG-4RFP

   Plasmid

   #52489

   Purpose

   Expresses TagRFP-T

   Depositor

   Sebastian Seung

   Article

   Nakashiba et al Cell. 2012 Mar 30;149(1):188-201. doi: 1

   Insert

   TagRFP-T
   

   Use

   Rabies virus

   Tags

   Expression

   Mutation

   Promoter

   CMV

   Available since

   Aug. 22, 2014

   Availability

   Academic Institutions and Nonprofits only

   

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9. pBEAST-pBen-sfGFP

   Plasmid

   #114598

   Purpose

   "Output expression of sfGFP under the activation of BenR transcription factor for cell-free expression"

   Depositor

   Jerome Bonnet

   Article

   Voyvodic et al Nat Commun. 2019 Apr 12;10(1):1697. doi:

   Insert

   sfGFP
   

   Use

   Synthetic Biology; Cell-free protein synthesis

   Tags

   Expression

   Mutation

   Promoter

   PBen

   Available since

   April 16, 2019

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

10. Delta DED FLIP-L

    Plasmid

    #13790

    Depositor

    Guy Salvesen

    Article

    Boatright et al Biochem J. 2004 Sep 1. 382(Pt 2):651-7.

    Insert

    DELTA DED FLIP LONG (CFLAR Human)
    

    Use

    Tags

    8 x His tag

    Expression

    Bacterial

    Mutation

    Delta DED removes first 186 residues

    Promoter

    Available since

    Jan. 25, 2007

    Availability

    Academic Institutions and Nonprofits only

    

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11. pBEAST-BenR

    Plasmid

    #114597

    Purpose

    "Strong constitutive expression of transcription factor, BenR, for cell-free expression"

    Depositor

    Jerome Bonnet

    Article

    Voyvodic et al Nat Commun. 2019 Apr 12;10(1):1697. doi:

    Insert

    BenR
    

    Use

    Synthetic Biology; Cell-free protein synthesis

    Tags

    Expression

    Mutation

    Promoter

    OR2-OR1-Pr

    Available since

    April 16, 2019

    Availability

    Academic Institutions and Nonprofits only

    

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12. pPKm-202

    Plasmid

    #90492

    Purpose

    Expresses pcDNA3 - CMVmin - 5X UAS - pFR - CMVmin Luciferase

    Depositor

    Todd Coleman

    Article

    Kyriakakis et al ACS Synth Biol. 2018 Feb 16;7(2):706-717

    Insert

    CMVmin Luciferase Fluc
    

    Use

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    Available since

    March 29, 2018

    Availability

    Academic Institutions and Nonprofits only

    

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13. pSilencer2.1-U6-3JB1F+2

    Plasmid

    #190833

    Purpose

    For mammalian expression of 3JB1F+2, a dual-specificity aptamer that binds OGT and GFP. Expression is driven by a U6 promoter. This dual-specificity aptamer adopts a folded linker.

    Depositor

    Gerald Hart

    Article

    Zhu et al Cell. 2022 Dec 30:S0092-8674(22)01529-X.

    Insert

    3JB1F_T1+2bp (A Dual-Specificity aptamer targeting OGT and GFP)
    

    Use

    Affinity Reagent/ Antibody and Synthetic Biology

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    U6

    Available since

    Jan. 9, 2023

    Availability

    Industry, Academic Institutions, and Nonprofits

    

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14. pPW3783 : CMVd3-ERmembrane-GST-2xHaloTag-KKMP

    Plasmid

    #185687

    Purpose

    Low-level transient expression of two tandem HaloTag proteins targeted to the ER membrane, fused with a GST for constitutive dimerization (making a HaloTag tetramer).

    Depositor

    Peter Walter

    Article

    Belyy et al Elife. 2022 Jun 22;11:e74342. doi: 10.75

    Insert

    ERmembrane-GST-2xHaloTag-KKMP
    

    Use

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    Available since

    March 14, 2023

    Availability

    Academic Institutions and Nonprofits only

    

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15. sgTrack-Gateway

    Plasmid

    #114011

    Purpose

    3rd generation lentiviral empty sgRNA expression vector with a Gateway cassette for co-expression of a reporter cDNA

    Depositor

    Charles Rudin

    Article

    Hulton et al Nat Cancer. 2020 Mar;1(3):359-369. doi:

    Type

    Empty backbone

    Use

    CRISPR and Lentiviral

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    Available since

    March 10, 2020

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

16. REEP5-2Strep

    Plasmid

    #210350

    Purpose

    Expresses REEP5 fused with 2Strep in mammalian cells

    Depositor

    Michele Pagano

    Article

    Li et al J Virol. 2023 Sep 28:e0050723. doi: 10.1

    Insert

    REEP5 (REEP5 Human)
    

    Use

    Tags

    2Strep

    Expression

    Mammalian

    Mutation

    Promoter

    Available since

    Dec. 19, 2023

    Availability

    Academic Institutions and Nonprofits only

    

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17. pJMP1217

    Plasmid

    #119258

    Purpose

    rfp "test" strain

    Depositor

    Carol Gross, Oren Rosenberg, Jason Peters

    Article

    Peters et al Nat Microbiol. 2019 Jan 7. pii: 10.1038/

    Insert

    sgRNA NT1/RR1 (rfp)
    

    Use

    Tags

    Expression

    Bacterial

    Mutation

    D10A and H840A

    Promoter

    Available since

    March 5, 2019

    Availability

    Academic Institutions and Nonprofits only

    

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18. pJH4260

    Plasmid

    #191678

    Purpose

    Ptwk-40s-GCaMP::wScarlet unc-54 3' UTR C.elegans AVA/other neurons expression GCaMP6s wScarlet

    Depositor

    Mei Zhen

    Article

    Lu et al Curr Biol. 2022 Nov 7;32(21):4631-4644.e

    Insert

    GCaMP6s
    

    Use

    Tags

    wScarlet

    Expression

    Worm

    Mutation

    Promoter

    Ptwk-40

    Available since

    Nov. 30, 2022

    Availability

    Industry, Academic Institutions, and Nonprofits

    

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19. pLX208-gLuc sp-NanoLuc-HA-oβ2AR-TS-eLOV-TEVcs-Flag-Gal4

    Plasmid

    #125230

    Purpose

    For generating SPARK NanoLuc-oβ2AR transmembrane component lentivirus

    Depositor

    Alice Ting

    Article

    Kim et al eLife. 2019 Apr 3;8. pii: 43826. doi: 10

    Insert

    gLuc sp-NanoLuc-HA-oβ2AR-TS-eLOV-TEVcs-Flag-Gal4
    

    Use

    Lentiviral

    Tags

    HA,Flag

    Expression

    Mammalian

    Mutation

    Promoter

    CMV

    Available since

    Sept. 6, 2019

    Availability

    Academic Institutions and Nonprofits only

    

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20. S12.1_Empty_Bridge

    Plasmid

    #127670

    Purpose

    Ampicillin resistant multiplexing backbone without CRY2HA and mCherry

    Depositor

    Jennifer Phillips-Cremins

    Article

    Kim et al Nat Methods. 2019 Jun 24. pii: 10.1038/s

    Type

    Empty backbone

    Use

    CRISPR

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    Available since

    June 27, 2019

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge