Skip to main content
Addgene
Open Menu

Enter a sequence to perform a BLAST-based search of Addgene plasmid sequence databases.

Information

In Category

Filter By

We narrowed to 1,138 results for: Tef

Clear keywords
Showing: 321 - 340 of 1138 results

  12. pCas9_sgRNA_0

    Plasmid
    #70763
    Purpose
    expresses Ustilago maydis codon-optimized Cas9, contains U. maydis U6 promoter, is self-replicating
    Depositor
    Regine Kahmann
    Article
    Schuster et al Fungal Genet Biol. 2015 Sep 11. pii: S10
    Inserts
    U6 promoter
    cas9
    tnos terminator
    Use
    Self-replicating in ustilago maydis, conferring c…
    Tags
    N-terminal NLS, C-terminal HA-tag +NLS
    Mutation
    Streptococcus pyogenes gene codon-optimized for U…
    Promoter
    U6 from Ustilago maydis and synthetic otef promot…
    Available Since
    Nov. 12, 2015
    Availability
    Academic Institutions and Nonprofits only
    70763-plasmid-map-sequence-id-240922

    Enlarge

  13. Cultivarium POSSUM Toolkit

    Plasmid Kit
    #1000000234
    Purpose
    Golden Gate genetic toolkit developed to help researchers identify functional plasmids and establish genetic tractability in non-model bacteria.
    Depositor
    Nili Ostrov
    Application
    Cloning and Synthetic Biology
    Vector Type
    Bacterial Expression
    Cloning Type
    Golden Gate (Loop)
    Available Since
    Feb. 23, 2024
    Availability
    Academic Institutions and Nonprofits only

  14. yeast_DualReporter

    Plasmid
    #127546
    Purpose
    A dual reporter vector modified from Sharon et al (Nature Biotech, 2012) containing Ura3 and Nat1 selectable markers, a constant background RFP (TEF2::mCherry), and a variable YFP (pTpA+MCS::yEVenus).
    Depositor
    Aviv Regev
    Article
    de Boer et al Nat Biotechnol. 2020 Jan;38(1):56-65. do
    Inserts
    bla
    mCherry
    URA3 (URA3 Budding Yeast)
    yEVenus
    Nat1
    Use
    Synthetic Biology
    Expression
    Yeast
    Promoter
    TEF, TEF2, URA3, bla (E. coli), and pTpA+MCS
    Available Since
    July 29, 2019
    Availability
    Academic Institutions and Nonprofits only
    127546-plasmid-map-sequence-id-251803

    Enlarge

  15. pRMH120

    Plasmid
    #40593
    Purpose
    Expresses Gal4BD-CRY2PHR and Gal4AD-CIB1 for light-inducible protein expression
    Depositor
    Chandra Tucker
    Article
    Hughes et al Methods. 2012 Aug 15.
    Inserts
    CRY2 (CRY2 Mustard Weed)
    CIB1 (CIB1 Mustard Weed)
    Expression
    Yeast
    Promoter
    ADH1 and TEF
    Available Since
    Oct. 29, 2012
    Availability
    Academic Institutions and Nonprofits only
    40593-plasmid-map-sequence-id-52556

    Enlarge

  18. pHS2

    Plasmid
    #81037
    Purpose
    HO endonuclease expression
    Depositor
    John McCusker
    Insert
    HYGMX
    Expression
    Yeast
    Promoter
    TEF
    Available Since
    Nov. 8, 2016
    Availability
    Academic Institutions and Nonprofits only
    81037-plasmid-map-sequence-id-274379

    Enlarge

  20. pAAH5

    Plasmid
    #41829
    Purpose
    Used as a PCR template for tagging yeast proteins with the SNAP tag by homologous recombination and selection for hygromycin resistance.
    Depositor
    Melissa Moore
    Article
    Hoskins et al Science. 2011 Mar 11;331(6022):1289-95.
    Type
    Empty backbone
    Use
    Pcr template
    Expression
    Bacterial and Yeast
    Promoter
    T7,TEF
    Available Since
    Oct. 17, 2013
    Availability
    Academic Institutions and Nonprofits only
    41829-plasmid-map-sequence-id-69202

    Enlarge

Showing: 321 - 340 of 1138 results