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pI-SceI

Plasmid

#243802

Purpose

Contains the ORF for the homing endonuclease I-SceI under the control of the strong, constitutive yeast promoter PTDH3.

Depositor

Sai Reddy

Article

Lester et al bioRxiv

Insert

I-SceI (SCEI Budding Yeast)

Expression

Yeast

Available Since

Oct. 8, 2025

Availability

Academic Institutions and Nonprofits only

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ISA179: Tagatose pathway

Plasmid

#241968

Purpose

Phagemid for the tagatose pathway. Contains p15A, CmR, pac site, AraC, C1, and pBAD.

Depositor

Nathan Crook

Article

Al'Abri et al Nucleic Acids Res. 2022 Jun 10;50(10):e5

Insert

Tagatose pathway

Use

Synthetic Biology; Phagemid

Expression

Bacterial

Available Since

Aug. 11, 2025

Availability

Academic Institutions and Nonprofits only

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lvl1-S5S6 [backbone]

Plasmid

#229198

Purpose

lvl 1 backbone

Depositor

Ron Weiss

Article

Duportet et al Nucleic Acids Res. 2014 Dec 1;42(21):134

Type

Empty backbone

Use

Synthetic Biology

Available Since

July 11, 2025

Availability

Academic Institutions and Nonprofits only

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pcDNA-mito-ApplePy1Lowest

Plasmid

#239077

Purpose

Mammalian expression of mitochondria-targeted lowest affinity red fluorescent pyruvate biosensor ApplePy1Lowest

Depositor

Robert Campbell

Article

Imai et al bioRxiv 2025.04.17.649293

Insert

ApplePy1Lowest

Expression

Mammalian

Available Since

June 25, 2025

Availability

Academic Institutions and Nonprofits only

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pcDNA-mito-ApplePy1Low

Plasmid

#239076

Purpose

Mammalian expression of mitochondria-targeted low affinity red fluorescent pyruvate biosensor ApplePy1Low

Depositor

Robert Campbell

Article

Imai et al bioRxiv 2025.04.17.649293

Insert

ApplePy1Low

Expression

Mammalian

Available Since

June 24, 2025

Availability

Academic Institutions and Nonprofits only

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pRK5_CKAR3

Plasmid

#238411

Purpose

Mammalian expression of CKAR3 under a CMV promotor.

Depositor

Haining Zhong

Article

Yahiro et al bioRxiv [Preprint]. 2024 Jul 23:2024.07.

Insert

CKAR3 (PKC sensor)

Expression

Mammalian

Available Since

June 3, 2025

Availability

Academic Institutions and Nonprofits only

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pRK5_mutCKAR3

Plasmid

#238412

Purpose

Mammalian expression of a phosphorylation deficient (T to A) mutant of CKAR3 under a CMV promotor.

Depositor

Haining Zhong

Article

Yahiro et al bioRxiv [Preprint]. 2024 Jul 23:2024.07.

Insert

phosphorylation deficient mutant of CKAR3 (T to A)

Expression

Mammalian

Available Since

June 3, 2025

Availability

Academic Institutions and Nonprofits only

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pSEVA63_sgRNA-B1_M13ps

Plasmid

#231422

Purpose

M13 phagemid that expresses sgRNA-B1 from a strong constitutive promoter. (This plasmid is a gift of the Jaramillo Lab, where it is called PAJ615.)

Depositor

Alfonso Jaramillo

Article

Kushwaha et al Zenodo (2017) Conference: SB7.0: The Sev

Insert

sgRNA-B1

Use

Synthetic Biology

Available Since

June 3, 2025

Availability

Academic Institutions and Nonprofits only

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lvl1-S2S3 [backbone]

Plasmid

#229195

Purpose

lvl 1 backbone

Depositor

Ron Weiss

Article

Duportet et al Nucleic Acids Res. 2014 Dec 1;42(21):134

Type

Empty backbone

Use

Synthetic Biology

Available Since

Feb. 21, 2025

Availability

Academic Institutions and Nonprofits only

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lvl1-S4S5 [backbone]

Plasmid

#229197

Purpose

lvl 1 backbone

Depositor

Ron Weiss

Article

Duportet et al Nucleic Acids Res. 2014 Dec 1;42(21):134

Type

Empty backbone

Use

Synthetic Biology

Available Since

Feb. 21, 2025

Availability

Academic Institutions and Nonprofits only

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lvl1-S3S4 [backbone]

Plasmid

#229196

Purpose

lvl 1 backbone

Depositor

Ron Weiss

Article

Duportet et al Nucleic Acids Res. 2014 Dec 1;42(21):134

Type

Empty backbone

Use

Synthetic Biology

Available Since

Feb. 21, 2025

Availability

Academic Institutions and Nonprofits only

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lvl2-[landingPad-backbone]

Plasmid

#229210

Purpose

lvl 1 backbone

Depositor

Ron Weiss

Article

Duportet et al Nucleic Acids Res. 2014 Dec 1;42(21):134

Type

Empty backbone

Use

Synthetic Biology

Available Since

Feb. 21, 2025

Availability

Academic Institutions and Nonprofits only

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lvl2-[piggyBac-backbone]

Plasmid

#229209

Purpose

lvl 1 backbone

Depositor

Ron Weiss

Article

Duportet et al Nucleic Acids Res. 2014 Dec 1;42(21):134

Type

Empty backbone

Use

Synthetic Biology

Available Since

Feb. 21, 2025

Availability

Academic Institutions and Nonprofits only

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pSEVA26_sgRNA-2_M13ps

Plasmid

#231412

Purpose

M13 phagemid that expresses sgRNA-2 from a strong constitutive promoter. (This plasmid is a gift of the Jaramillo Lab, where it is called PAJ171.)

Depositor

Alfonso Jaramillo

Article

Kushwaha et al Zenodo (2017) Conference: SB7.0: The Sev

Insert

sgRNA-2

Use

Synthetic Biology

Available Since

Feb. 19, 2025

Availability

Academic Institutions and Nonprofits only

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pSEVA26_sgRNA-A1_M13ps

Plasmid

#231414

Purpose

M13 phagemid that expresses sgRNA-A1 from a strong constitutive promoter. (This plasmid is a gift of the Jaramillo Lab, where it is called PAJ164.)

Depositor

Alfonso Jaramillo

Article

Kushwaha et al Zenodo (2017) Conference: SB7.0: The Sev

Insert

sgRNA-A1

Available Since

Feb. 19, 2025

Availability

Academic Institutions and Nonprofits only

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lvl1-S1S2 [backbone]

Plasmid

#229194

Purpose

lvl 1 backbone

Depositor

Ron Weiss

Article

Duportet et al Nucleic Acids Res. 2014 Dec 1;42(21):134

Type

Empty backbone

Use

Synthetic Biology

Available Since

Jan. 30, 2025

Availability

Academic Institutions and Nonprofits only

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lvl2-[lenti-backbone]

Plasmid

#229208

Purpose

lvl 1 backbone

Depositor

Ron Weiss

Article

Duportet et al Nucleic Acids Res. 2014 Dec 1;42(21):134

Type

Empty backbone

Use

Synthetic Biology

Available Since

Jan. 30, 2025

Availability

Academic Institutions and Nonprofits only

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lvl0-I-E1

Plasmid

#229161

Purpose

lvl 0 insulator

Depositor

Ron Weiss

Article

Duportet et al Nucleic Acids Res. 2014 Dec 1;42(21):134

Insert

E1

Use

Synthetic Biology

Available Since

Jan. 30, 2025

Availability

Academic Institutions and Nonprofits only

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lvl0-I-F1

Plasmid

#229162

Purpose

lvl 0 insulator

Depositor

Ron Weiss

Article

Duportet et al Nucleic Acids Res. 2014 Dec 1;42(21):134

Insert

F1

Use

Synthetic Biology

Available Since

Jan. 30, 2025

Availability

Academic Institutions and Nonprofits only

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lvl0-I [backbone]

Plasmid

#229153

Purpose

Insulator backbone

Depositor

Ron Weiss

Article

Duportet et al Nucleic Acids Res. 2014 Dec 1;42(21):134

Type

Empty backbone

Use

Synthetic Biology

Available Since

Jan. 30, 2025

Availability

Academic Institutions and Nonprofits only

Enlarge