We narrowed to 13,173 results for: transfer

Showing: 5191 - 5220 of 13173 results

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pAAV.minBG-CI-SaCas9-W3SynpA

Plasmid

#231369

Purpose

minBG-driven SaCas9, with short synthetic terminator sequence.

Depositor

Viviana Gradinaru

Article

Coughlin et al Nat Biotechnol. 2025 Mar 20. doi: 10.103

Insert

SaCas9

Use

AAV

Available Since

March 20, 2025

Availability

Academic Institutions and Nonprofits only

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pAAV-TRE-DIO-mTurquoise2

Plasmid

#99115

Purpose

An AAV genome with tet-inducible, Cre-dependent expression of the fluorescent protein mTurquoise2

Depositor

Viviana Gradinaru

Article

Chan et al Nat Neurosci. 2017 Aug;20(8):1172-1179.

Insert

DIO-mTurquoise2

Use

AAV

Expression

Mammalian

Promoter

TRE

Available Since

Aug. 16, 2017

Availability

Academic Institutions and Nonprofits only

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pAc-His-hAR-2

Plasmid

#89091

Purpose

baculovirus transfer vector for histidine-tagged full-length human androgen receptor, starts Met-Ala-His6-Met-hAR

Depositor

Elizabeth Wilson

Article

Liao et al Biochemistry. 1999 Jul 27;38(30):9718-27

Insert

hAR (AR Human)

Tags

His

Expression

Mammalian

Available Since

April 19, 2017

Availability

Academic Institutions and Nonprofits only
pAAV2-SynTetOff-myrGFP

Plasmid

#190233

Purpose

AAV vector, high-level expression of EGFP with a myristoylation/palmitoylation signal derived from Fyn N-terminus (myrGFP) in neuronal cells

Depositor

Hiroyuki Hioki

Article

Sohn et al PLoS One. 2017 Jan 6;12(1):e0169611. doi

Insert

EGFP

Use

AAV

Tags

myristoylation signal sequence

Expression

Mammalian

Available Since

Jan. 10, 2023

Availability

Academic Institutions and Nonprofits only

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pAAV.hSyn-FLEX.iGABASnFR.F102G

Plasmid

#112164

Purpose

GABA Sensor

Depositor

Loren Looger

Article

Marvin et al Nat Methods. 2019 Aug;16(8):763-770. doi

Has Service

AAV1

Insert

iGABASnFR.F102G

Use

AAV

Mutation

F102G

Promoter

Synapsin

Available Since

July 16, 2018

Availability

Academic Institutions and Nonprofits only

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pAAV-CAG-Flex-mRuby2-GSG-P2A-GCaMP6s-WPRE-pA

Plasmid

#68717

Purpose

Cre-dependent bicistronic vector expressing mRuby2 and GCaMP6s from a single open reading frame.

Depositor

Tobias Bonhoeffer, Mark Huebener, Tobias Rose

Article

Rose et al Science. 2016 Jun 10;352(6291):1319-22.

Has Service

AAV1

Insert

mRuby2-P2A-GCaMP6s

Use

AAV

Mutation

Cre-dependent expression from inverted open readi…

Promoter

CAG-FLEX

Available Since

June 20, 2016

Availability

Academic Institutions and Nonprofits only

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pM125: pAAV-EFS-CasRx-VEGFA array presgRNA

Plasmid

#166873

Purpose

AAV vector for expressing CasRx and human VEGFA targeting presgRNA array for RNA-editing

Depositor

Guei-Sheung Liu

Article

Chuang et al Front Cell Dev Biol. 2021 Jun 11;9:66787

Inserts

U6-VEGFA array presgRNA
RfxCas13d

Use

AAV and CRISPR

Tags

HA and NLS

Expression

Mammalian

Promoter

EFS and U6

Available Since

July 1, 2021

Availability

Academic Institutions and Nonprofits only

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pTS100 pHAGE2 CMVtetO2 TagBFP-P2A-ALFA-22xGS-TEV-MCS

Plasmid

#199353

Purpose

Lentiviral transfer plasmid for doxycycline inducible expression of a N-terminally BFP-P2A-ALFA-TEV-tagged protein in mammalian cells.

Depositor

Rebecca Voorhees

Article

Stevens et al Nat Protoc. 2024 Jan;19(1):127-158. doi:

Type

Empty backbone

Use

Lentiviral

Tags

TagBFP-P2A-ALFA-TEV

Expression

Mammalian

Mutation

WT

Available Since

May 23, 2023

Availability

Academic Institutions and Nonprofits only

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pAAV.CAG.GCaMP6m.WPRE.SV40

Plasmid

#100840

Purpose

AAV expression of ultrasensitive protein calcium sensor from CAG promoter

Depositor

Douglas Kim, GENIE Project

Article

Chen et al Nature. 2013 Jul 18;499(7458):295-300. d

Has Service

AAV1

Insert

GCaMP6m

Use

AAV

Expression

Mammalian

Mutation

GCaMP3-T302L R303P M378G K379S D380Y T381R S383T …

Promoter

CAG

Available Since

Nov. 9, 2017

Availability

Academic Institutions and Nonprofits only

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pAC8_MF (VE5596)

Plasmid

#139768

Purpose

Transfer vector for multigene expression to generate recombinant baculoviruses by homologous recombination. Contains a p10/pH dual expression cassette.

Depositor

Arnaud Poterszman

Article

Kolesnikova et al Sci Rep. 2022 Feb 7;12(1):2030. doi: 10.

Type

Empty backbone

Promoter

PH or p10

Available Since

May 19, 2022

Availability

Academic Institutions and Nonprofits only

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pAAV-CAG-EBFP2-P2A-EGFP

Plasmid

#184938

Purpose

Expresses blue fluorescent protein EBFP2 and EGFP in mammalian cells

Depositor

Kiryl Piatkevich

Article

Papadaki et al Sci Rep. 2022 Jun 17;12(1):10190. doi: 1

Insert

EBFP2-P2A-EGFP

Use

AAV

Expression

Mammalian

Promoter

CAG

Available Since

Sept. 7, 2022

Availability

Academic Institutions and Nonprofits only

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pAAV-hSyn-eGFP-P2A-LgBiT-NLS

Plasmid

#187834

Purpose

Expression of eGFP-P2A-LgBiT-NLS from human synapsin promoter via adeno-associated virus for neuronal expression.

Depositor

William McEwan

Article

Tuck et al Cell Rep. 2022 May 3;39(5):110776. doi:

Insert

eGFP-P2A-LgBiT-NLS

Use

AAV

Promoter

hSyn

Available Since

Sept. 22, 2023

Availability

Academic Institutions and Nonprofits only

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pAAV_AR-RE_MLPmin_BC1014-luc2

Plasmid

#227108

Purpose

Androgen receptor response element for luciferase and barcode assays; barcode BC1014

Depositor

Michael Wehr

Article

Popovic et al Biomed Pharmacother. 2024 Nov;180:117523

Insert

8x clustered AR response element linked to MLP minimal promoter driving barcode 1014 and a luciferase reporter gene

Use

AAV

Expression

Mammalian

Available Since

Nov. 26, 2024

Availability

Academic Institutions and Nonprofits only

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PP_080_pAAV_CAG_DIO-LR-Voltron2-P2A-CheRiff-eGFP-ST (Kv2.1TS)

Plasmid

#230990

Purpose

Co-expressing membrane-localized Voltron2 and membrane-localized CheRiff.

Depositor

Adam Cohen

Article

Wong-Campos et al bioRxiv [Preprint]. 2023 May 26:2023.05.

Insert

Cre-dependent, membrane-localized Voltron2 and soma-localized CheRiff

Use

AAV

Expression

Mammalian

Promoter

CAG

Available Since

June 5, 2025

Availability

Academic Institutions and Nonprofits only

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pAAV-CAG-EGFP-P2A-FusionRed

Plasmid

#191108

Purpose

Mammalian cell line expression plasmids for production of EGFP and FusionRed proteins

Depositor

Kiryl Piatkevich

Article

Liang et al Front Bioeng Biotechnol. 2022 Oct 17;10:

Insert

pAAV-CAG-EGFP-P2A-FusionRed

Use

AAV

Tags

FusionRed

Expression

Mammalian

Mutation

See depositor comments below

Promoter

chicken β-actin promoter

Available Since

Nov. 6, 2024

Availability

Academic Institutions and Nonprofits only

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AAV CMV-driven AcrIIC3-scaffold (2xBsmBI sites)

Plasmid

#120301

Purpose

AAV vector for expression of AcrIIC3 (no miR binding sites, control vector)

Depositor

Dominik Niopek

Article

Hoffmann et al Nucleic Acids Res. 2019 Apr 15. pii: 545

Insert

AcrIIC3

Use

AAV and CRISPR

Expression

Mammalian

Available Since

Jan. 23, 2019

Availability

Academic Institutions and Nonprofits only

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pAAV-CaMKII-VARNAM2-Kv2.1PR

Plasmid

#195530

Purpose

Red fluorescent, negative response-polarity voltage indicator under the control of CaMKII promoter; soma-targeted

Depositor

Vincent Pieribone

Article

Kannan et al Science. 2022 Nov 4;378(6619):eabm8797.

Insert

VARNAM2-Kv2.1 proximal restriction sequence

Use

AAV

Expression

Mammalian

Mutation

VARNAM SI linker

Promoter

CaMKII

Available Since

Jan. 31, 2023

Availability

Academic Institutions and Nonprofits only

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pAAV-CAG-fDIO-VARNAM2-Kv2.1PR

Plasmid

#195536

Purpose

Flp recombinase-dependent red fluorescent, negative response-polarity voltage indicator; soma-targeted

Depositor

Vincent Pieribone

Article

Kannan et al Science. 2022 Nov 4;378(6619):eabm8797.

Insert

VARNAM2-Kv2.1 proximal restriction sequence

Use

AAV

Expression

Mammalian

Mutation

VARNAM SI linker

Promoter

CAG

Available Since

Jan. 31, 2023

Availability

Academic Institutions and Nonprofits only

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pAAV_ER-RE_MLPmin_BC1063-luc2

Plasmid

#227109

Purpose

Estrogen receptor response element for luciferase and barcode assays; barcode BC1063

Depositor

Michael Wehr

Article

Popovic et al Biomed Pharmacother. 2024 Nov;180:117523

Insert

12x clustered ER response element linked to MLP minimal promoter driving barcode 1063 and a luciferase reporter gene

Use

AAV

Expression

Mammalian

Available Since

Nov. 26, 2024

Availability

Academic Institutions and Nonprofits only

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pAAV-CaMKII-JEDI-1P-WPRE

Plasmid

#202615

Purpose

Genetically encoded voltage indicator (GEVI) JEDI-1P in AAV production vector for expression in excitatory glutamatergic neurons using the promoter CaMKII

Depositor

Francois St-Pierre

Article

Lu et al Nat Commun. 2023 Oct 12;14(1):6423. doi:

Insert

JEDI-1P

Use

AAV

Expression

Mammalian

Promoter

CaMKIIa

Available Since

June 22, 2023

Availability

Academic Institutions and Nonprofits only

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pAAV-CaMKII-STChRger2-TS-EYFP

Plasmid

#129393

Purpose

Soma Targeted, High photocurrent, low-light sensitive channelrhodopsin (ChRger2) for optogenetic activation with systemic delivery or for low-light activation. Driven by the CamKIIa promoter.

Depositor

Viviana Gradinaru

Article

Bedbrook et al Nat Methods. 2019 Oct 14. pii: 10.1038/s

Insert

soma targeted ChRger2

Use

AAV

Tags

Kv2.1-TS-EYFP

Expression

Mammalian

Promoter

CaMKIIa

Available Since

Nov. 5, 2019

Availability

Academic Institutions and Nonprofits only

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pAAV-EF1a-HA-hTet1CDmu-WPRE-PolyA

Plasmid

#39455

Depositor

Hongjun Song

Article

Guo et al Cell. 2011 Apr 29;145(3):423-34. Epub 20

Insert

HA-hTet1CDmu (TET1 Human)

Use

AAV

Tags

HA

Expression

Mammalian

Mutation

aa1418–2136 with mutations H1672 to Y, 1674D to A

Promoter

EF1a

Available Since

Aug. 22, 2012

Availability

Academic Institutions and Nonprofits only
AAV-hSyn-FlicR1

Plasmid

#74143

Purpose

Express FlicR1 preferentially in neurons

Depositor

Robert Campbell

Article

Abdelfattah et al J Neurosci. 2016 Feb 24;36(8):2458-72. d

Insert

FlicR1

Use

AAV

Expression

Mammalian

Promoter

hSyn

Available Since

April 19, 2016

Availability

Academic Institutions and Nonprofits only

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pAAV-hSyn-sbGLuc-ChR2(CS/DA)-EYFP

Plasmid

#114106

Purpose

fusion protein of Gaussia luciferase variant slow burn, Channelrhodopsin-2 variant C128S/D156A (stabilized step function opsin), and enhanced yellow fluorescent protein for bioluminescent optogenetics

Depositor

Ute Hochgeschwender

Article

Berglund et al J Neurosci Res. 2019 Apr 7. doi: 10.1002

Insert

Gaussia luciferase, slow burn; Channelrhodopsin 2(CS/DA), stable step function opsin; EYFP

Use

AAV

Expression

Mammalian

Promoter

hSyn

Available Since

Aug. 6, 2019

Availability

Academic Institutions and Nonprofits only

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pAAV2-SynTetOff-FGL

Plasmid

#190234

Purpose

AAV vector, high-level expression of myrGFP with a somatodendritic-targeting signal (FGL) in neuronal cells

Depositor

Hiroyuki Hioki

Article

Sohn et al PLoS One. 2017 Jan 6;12(1):e0169611. doi

Insert

EGFP

Use

AAV

Tags

LDLR C-terminal sequence and myristoylation signa…

Expression

Mammalian

Available Since

Nov. 10, 2022

Availability

Academic Institutions and Nonprofits only

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pAAV-Syn-pAceR-Kv2.1PR

Plasmid

#195525

Purpose

Red fluorescent, positive response-polarity voltage indicator under the control of synapsin promoter; soma-targeted

Depositor

Vincent Pieribone

Article

Kannan et al Science. 2022 Nov 4;378(6619):eabm8797.

Insert

pAceR-Kv2.1 proximal restriction sequence

Use

AAV

Expression

Mammalian

Mutation

VARNAM 78E, 81D, 92N; SI linker

Promoter

Synapsin

Available Since

Jan. 30, 2023

Availability

Academic Institutions and Nonprofits only

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pAV-CAG-oScarlet-WPRE-BC5-pA

Plasmid

#220769

Purpose

Expression of oScarlet for cell isolation and Projection-TAG BC for multiplex projection tracing

Depositor

Vijay Samineni

Article

Yang et al Nat Commun. 2025 Jul 1;16(1):5557. doi:

Insert

oScarlert-WPRE-BC5

Use

AAV

Promoter

CAG

Available Since

Jan. 13, 2025

Availability

Academic Institutions and Nonprofits only

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pAV-CAG-oScarlet-WPRE-BC4-pA

Plasmid

#220768

Purpose

Expression of oScarlet for cell isolation and Projection-TAG BC for multiplex projection tracing

Depositor

Vijay Samineni

Article

Yang et al Nat Commun. 2025 Jul 1;16(1):5557. doi:

Insert

oScarlert-WPRE-BC4

Use

AAV

Promoter

CAG

Available Since

Jan. 13, 2025

Availability

Academic Institutions and Nonprofits only

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pAV-CAG-oScarlet-WPRE-BC3-pA

Plasmid

#220767

Purpose

Expression of oScarlet for cell isolation and Projection-TAG BC for multiplex projection tracing

Depositor

Vijay Samineni

Article

Yang et al Nat Commun. 2025 Jul 1;16(1):5557. doi:

Insert

oScarlert-WPRE-BC3

Use

AAV

Promoter

CAG

Available Since

June 13, 2024

Availability

Academic Institutions and Nonprofits only

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pAAV_hSyn_SpikeyGi2_kv2.1

Plasmid

#195313

Purpose

Genetically-encoded voltage indicator SpikeyGi2, soma and AIS targeted, in AAV vector under the neuronal-specific promoter hSyn

Depositor

Vincent Pieribone

Article

Platisa et al Nat Methods. 2023 Mar 27. doi: 10.1038/s

Insert

SpikeyGi2

Use

AAV

Promoter

hSyn

Available Since

March 27, 2023

Availability

Academic Institutions and Nonprofits only

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