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Showing: 3481 - 3500 of 25179 results

  4. pET24a-VHH-APEX2

    Plasmid
    #109420
    Purpose
    Bacterial expression of a functionalized anti-GFP (VHH) nanobody fused to ascorbate peroxidase 2. VHH-APEX2 also contains a T7, HA, BAP and His6 epitope
    Depositor
    Martin Spiess
    Publication
    Buser et al Proc Natl Acad Sci U S A. 2018 Jul 3;115
    Insert
    anti-GFP nanobody fused to a T7, APEX2, HA, BAP and His6 epitope
    Tags
    BAP, HA, His6, and T7
    Expression
    Bacterial
    Promoter
    T7
    Available since
    June 19, 2018
    Availability
    Academic Institutions and Nonprofits only
    109420-plasmid-map-sequence-id-211586

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  6. pTriEx-PA-Rac1-I539E

    Plasmid
    #22026
    Purpose
    Expression of photoactivatable Rac1 ('lit' state mutant)
    Depositor
    Klaus Hahn
    Publication
    Wu et al Nature. 2009 Sep 3. 461(7260):104-8.
    Insert
    PA-Rac1-I539E (RAC1 Human, Avena sativa (oat))
    Tags
    6X His
    Expression
    Bacterial, Insect, and Mamm…
    Mutation
    LOV domain contains 'lit state' mutatio…
    Promoter
    CMV, p10
    Available since
    Sept. 29, 2009
    Availability
    Academic Institutions and Nonprofits only
    Cited by
    2 Articles

  11. pCVL.SFFV.Kozak.HA.NLS.SceOpt.IRES.BFP

    Plasmid
    #45574
    Purpose
    Expresses I-Sce I nuclease in mammalian cells with BFP tracker in lentiviral backbone
    Depositor
    Andrew Scharenberg
    Publication
    Kuhar et al Nucleic Acids Res. 2013 Oct 10.
    Insert
    co I-Sce I
    Use
    Lentiviral
    Tags
    HA, IRES BFP, and NLS
    Expression
    Mammalian
    Mutation
    co indicates codon optimization for mammalian exp…
    Promoter
    SFFV
    Available since
    Nov. 4, 2013
    Availability
    Academic Institutions and Nonprofits only
    Cited by
    4 Articles
    45574-plasmid-map-sequence-id-67191

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  15. Dead Streptavidin-SpyCatcher

    Plasmid
    #59547
    Purpose
    pET21-DCatch encodes Dead streptavidin (negligible biotin binding) bearing a C-terminal SpyCatcher, for generation of chimeric SpyAvidin tetramers
    Depositor
    Mark Howarth
    Publication
    Fairhead et al J Am Chem Soc. 2014 Sep 3;136(35):12355-
    Insert
    Dead-dloop-Streptavidin-SpyCatcher
    Tags
    N-terminally shortened SpyCatcher
    Expression
    Bacterial
    Mutation
    PolyAsp in L3/4 loop for ion-exchange
    Available since
    Oct. 3, 2014
    Availability
    Academic Institutions and Nonprofits only
    Cited by
    4 Articles
Showing: 3481 - 3500 of 25179 results