We narrowed to 7,945 results for: lif
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Plasmid#122151PurposeExpresses dCas9-SunTag(24X) fusion and a fluorescent indicator BFP.DepositorInsertdCas9 fused to SunTag(24x)
UseLentiviralTags2xNLS-P2A-BFP and NLSExpressionMammalianPromoterSFFVAvailable SinceMarch 6, 2019AvailabilityAcademic Institutions and Nonprofits only -
pDONR P2R-P3-SV40 polyA signal
Plasmid#48627PurposeContributes a cassette containing an SV40 polyA signal as the 3’-module during MultiSite Gateway cloning of chimeric cDNAs, when a peptide module at the C-terminal end is not required.DepositorInsertSV40 early polyadenylation signal cassette
UseGateway entry vectorPromoternoneAvailable SinceApril 9, 2014AvailabilityAcademic Institutions and Nonprofits only -
pRPN265NB-enh-MNase
Plasmid#166035PurposeExpresses a nanobody against GFP fused to micrococcal nuclease for purposes of green Cut & RunDepositorInsertGlutathione-S-Transferase/enhancer/MNase
TagsGSTExpressionBacterialPromoterlac promoter / tac promoterAvailable SinceApril 16, 2021AvailabilityAcademic Institutions and Nonprofits only -
pR26
Plasmid#127372PurposeAllows for doxycycline-inducible gene expression from the murine ROSA26 safe harbor locus upon CRISPR/Cas9-mediated genomic insertion and stable selection.DepositorTypeEmpty backboneUseCRISPR and Mouse TargetingPromotertight TRE promoterAvailable SinceFeb. 13, 2020AvailabilityAcademic Institutions and Nonprofits only -
pcDNA3-CaMPARI V398D
Plasmid#60786PurposeExpresses CaMPARI V398D in mammalian cells, a photoconvertible fluorescent protein-based calcium integratorDepositorInsertCaMPARI V398D
TagsNuclear Export SignalExpressionMammalianMutationV398DPromoterCMVAvailable SinceNov. 26, 2014AvailabilityAcademic Institutions and Nonprofits only -
pR26-CMVconst
Plasmid#127373PurposeAllows for constitutive gene expression from the murine ROSA26 safe harbor locus upon CRISPR/Cas9-mediated genomic insertion and stable selection.DepositorTypeEmpty backboneUseCRISPR and Mouse TargetingPromoterCMV enhancer/promoterAvailable SinceFeb. 13, 2020AvailabilityAcademic Institutions and Nonprofits only -
pJRoC30_Tv9nL
Plasmid#188069PurposePROSS-designed high-redox potential laccase from Trametes versicolorDepositorInsertPROSS-designed high-redox potential laccase from Trametes versicolor
TagsS. cerevisiae evolved α-factor prepro-leader sign…ExpressionYeastMutation79 PROSS mutations, described in publicationAvailable SinceOct. 24, 2024AvailabilityAcademic Institutions and Nonprofits only -
MSCV-Luciferase-IRES-NrasG12D
Plasmid#60834PurposeMouse NrasG12D was amplified and mutated by PCR from IMAGE clone 6475312 (Invitrogen) and cloned downstream from the IRES; subsequently Luciferase was cloned upstream of the IRESDepositorInsertNrasG12D (Nras Mouse)
UseRetroviralAvailable SinceNov. 13, 2014AvailabilityAcademic Institutions and Nonprofits only -
pBAD-Padron2
Plasmid#172355PurposeExpresses the reversibly switchable fluorescent protein Padron2 in bacterial cellsDepositorInsertPadron2
Tags6xHisTag (N terminal on backbone)ExpressionBacterialPromoteraraBADAvailable SinceMarch 17, 2022AvailabilityAcademic Institutions and Nonprofits only -
pAAV-TRE-fDIO-mem-seTurboID-IRES-tTA
Plasmid#237889PurposeAmplifier AAV vector of Dual-AAV sparse labeling system encoding membrane-tethered seTurboIDDepositorInsertmem-seTurboID
UseAAVTagsFLAG tag and GAP43 palmitoylation signalExpressionMammalianPromoterTREAvailable SinceAug. 25, 2025AvailabilityAcademic Institutions and Nonprofits only -
pBAD miniSOG
Plasmid#91774PurposeBacterial expression of a fluorescent protein, mini Singlet Oxygen Generator (miniSOG)DepositorInsertmini Singlet Oxygen Generator
TagsHexa-Histidine tagExpressionBacterialPromoteraraBADAvailable SinceJune 27, 2017AvailabilityAcademic Institutions and Nonprofits only -
pDONR P2R-P3-mKate2
Plasmid#48345PurposeContributes the coding sequence for the mKate2 fluorescent protein as the 3’-module during MultiSite Gateway-cloning of chimeric cDNAs encoding three-part fusion proteins.DepositorInsertmKate2
UseGateway entry vectorMutationContains a stop codon at the 3' end of the c…PromoternoneAvailable SinceApril 9, 2014AvailabilityAcademic Institutions and Nonprofits only -
pDONR P2R-P3-EYFP
Plasmid#48351PurposeContributes the coding sequence for EYFP as the 3’-module during MultiSite Gateway-cloning of chimeric cDNAs encoding three-part fusion proteins.DepositorInsertEYFP
UseGateway entry vectorMutationContains a stop codon.PromoternoneAvailable SinceApril 9, 2014AvailabilityAcademic Institutions and Nonprofits only -
pDEST14-ARL8B
Plasmid#67383PurposeExpression of native hArl8b in E. coliDepositorAvailable SinceOct. 5, 2015AvailabilityAcademic Institutions and Nonprofits only -
pDEST14-ARL3
Plasmid#67376PurposeExpression of native hArl3 in E. coliDepositorAvailable SinceOct. 5, 2015AvailabilityAcademic Institutions and Nonprofits only -
pAAV_Thy1StTA
Plasmid#97411PurposeTET driver for amplified expression in cortical neuronsDepositorInserttTA
UseAAVExpressionMammalianPromotermouse thy1PSsAvailable SinceJan. 9, 2018AvailabilityAcademic Institutions and Nonprofits only -
pMRX-IB-mGFP-FTH1
Plasmid#192800PurposeStable expression of mGFP-FTH1 in mammalian cellsDepositorAvailable SinceDec. 6, 2022AvailabilityAcademic Institutions and Nonprofits only -
pcDNA3.1(+)-MC38-TCRA-30-picky_sticky
Plasmid#138983PurposeIVT template for the alpha subunit of the mouse TCR #30 that is reactive against MC38DepositorAvailable SinceNov. 24, 2021AvailabilityAcademic Institutions and Nonprofits only -
pAAVTREGCaMP6f
Plasmid#97410PurposeAmplified expression of GCaMP6f in combination with TET driverDepositorInsertGCaMP6f
UseAAVExpressionMammalianPromoterTRE3Available SinceJan. 11, 2018AvailabilityAcademic Institutions and Nonprofits only -
pAAV-ihSyn1-DIO-tTA
Plasmid#99121PurposeAn AAV genome with Cre-dependent expression of the tTA from the hSyn1 promoter. Contains a positive feedback loop for amplifed expression of the tTADepositorHas ServiceAAV1InsertDIO-tTA
UseAAVExpressionMammalianMutation*(see below)PromoterihSyn1Available SinceSept. 15, 2017AvailabilityAcademic Institutions and Nonprofits only -
HRAS
Plasmid#55653Purposeexpression of mouse HRASDepositorAvailable SinceAug. 21, 2014AvailabilityAcademic Institutions and Nonprofits only -
pWPT-/GCCACC-mEGFP-IRES-mCherry
Plasmid#49235PurposeLentiviral expression vector. Contains an altered Kozak sequence and/or upstream open reading frames to modulate expression at the level of translation.DepositorInsertsmEGFP
mCherry
UseLentiviral and Synthetic BiologyTagsSfuI site to create C-terminal fusionsExpressionMammalianPromoterEF1-shortAvailable SinceDec. 5, 2013AvailabilityAcademic Institutions and Nonprofits only -
GFP-CYLD-STOP
Plasmid#60077PurposeN-terminal GFP tagged CYLD with stop codon, driven by CMV promoterDepositorAvailable SinceAug. 26, 2015AvailabilityAcademic Institutions and Nonprofits only -
pBAD IFP1.4
Plasmid#91775PurposeBacterial expression of a fluorescent protein, Infrared Fluorescent Protein 1.4 + Heme oxygenase-1 (IFP1.4)DepositorInsertInfrared Fluorescent Protein 1.4 + Heme oxygenase-1
TagsHexa-Histidine tagExpressionBacterialPromoteraraBADAvailable SinceJune 30, 2017AvailabilityAcademic Institutions and Nonprofits only -
pTRE-Tight-FLAG-CYLD-WT
Plasmid#60030PurposeWT CYLD under control of Tet promoter with N-terminal FLAG tagDepositorAvailable SinceNov. 25, 2014AvailabilityAcademic Institutions and Nonprofits only -
GST-Delta23C11orf83
Plasmid#65849Purposebacterial expression of GST (N-ter) - Delta 23 C11orf83/UQCC3 (UQCC3 protein depleted of its N-terminal transmembrane domain)DepositorInsertUQCC3 depleted of its N terminal transmembrane part (UQCC3 Human)
TagsGSTExpressionBacterialMutationdeletion of the 23 first aa (TM)Available SinceJuly 7, 2015AvailabilityAcademic Institutions and Nonprofits only -
pcDNA3-FLAG-VSVG
Plasmid#80606PurposeMammalian expression vector that expresses a VSV-G protein with a FLAG tag near the N-terminus of the proteinDepositorInsertVSV-G
TagsFLAGExpressionMammalianPromoterCMVAvailable SinceAug. 23, 2016AvailabilityAcademic Institutions and Nonprofits only -
Hsp70-mplum
Plasmid#45016DepositorInsertmPlum
ExpressionMammalianPromotermouse Hspa1bAvailable SinceMay 24, 2013AvailabilityAcademic Institutions and Nonprofits only -
pDest27-mBIN1+13+17
Plasmid#61097PurposeExpress N-terminal GST tagged-mouse BIN1+13+17 in mammalian cellsDepositorInsertmouse BIN1 with exon 13 and 17, excluding exon 7, 11, 14-16
TagsGSTExpressionMammalianPromoterCMVAvailable SinceJan. 23, 2015AvailabilityAcademic Institutions and Nonprofits only -
pLenti-SPA2G231V
Plasmid#42079DepositorInsertSurfactant Protein -A2 (SFTPA2 Human)
UseLentiviral; PlentiTagsV5ExpressionBacterial and MammalianMutationChanged Glycine 231 to ValinePromoterCMVAvailable SinceMarch 19, 2013AvailabilityAcademic Institutions and Nonprofits only -
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pDONR P4-P1R-ECFP
Plasmid#48352PurposeContributes the coding sequence for ECFP as the 5’-module during MultiSite Gateway-cloning of chimeric cDNAs encoding three-part fusion proteins.DepositorInsertECFP
UseGateway entry vectorMutationContains a Kozak sequence. Does not contain a sto…PromoternoneAvailable SinceApril 9, 2014AvailabilityAcademic Institutions and Nonprofits only -
Myc-CYLD-C601A
Plasmid#60027PurposeMyc-tagged CYLD catalytic inactive (C601A) expressionDepositorInsertCylindromatosis (CYLD Human)
TagsMycExpressionMammalianMutationCysteine 601 changed to AlaninePromoterCMVAvailable SinceNov. 25, 2014AvailabilityAcademic Institutions and Nonprofits only -
pME GFP-PCNA
Plasmid#105977Purposeentry clone for gateway cloning, cell cycle marker proliferative cell nuclear antigen PCNADepositorAvailable SinceFeb. 6, 2018AvailabilityAcademic Institutions and Nonprofits only -
pMAL-MBP-Cdc42-Q61L
Plasmid#214288PurposeExpress constitutively active Cdc42 fused with MBPDepositorInsertConstitutively active Cdc42 (CDC42 Human)
TagsMaltose-binding protein (MBP)ExpressionBacterialMutationCdc42-Q61LPromoterTacAvailable SinceJuly 29, 2024AvailabilityAcademic Institutions and Nonprofits only -
Cav2.2 HA pcDNA3
Plasmid#206051Purposeexpression of rabbit Cav2.2 calcium channel with an exofacial double HA tag in domain IIDepositorAvailable SinceSept. 14, 2023AvailabilityAcademic Institutions and Nonprofits only -
L-GARS-2
Plasmid#166531PurposescFv of a human scaffold targeting Glycyl-tRNA synthetase. Antigen coverage aa 55-739 of 739DepositorInsertsingle chain-Fv, scFv
UseRecombinant antibody expressionTags3xFLAG, His6 and OmpA leader sequenceExpressionBacterialPromoterLacZAvailable SinceMarch 24, 2021AvailabilityAcademic Institutions and Nonprofits only -
pJRoC30_Th3
Plasmid#188064PurposePROSS-designed high-redox potential laccase from Trametes hirsutaDepositorInsertPROSS-designed high-redox potential laccase from Trametes hirsuta
TagsS. cerevisiae evolved α-factor prepro-leader sign…ExpressionYeastMutation20 mutations, described in publicationAvailable SinceOct. 24, 2024AvailabilityAcademic Institutions and Nonprofits only -
pGS1021
Plasmid#59575PurposeVector to create a cassette for homologous recombination in S. cerevisiae; adds a C-terminal Twin-Strep tag and a TRP1 selection markerDepositorInsertTwin-Strep tag optimized for PCR amplification followed by TRP1 selection marker
UseE. coli cloning vectorTagsTwin-Strep tagPromoterNoneAvailable SinceAug. 28, 2014AvailabilityAcademic Institutions and Nonprofits only -
pGG-NES-TurboID-GFP
Plasmid#209401PurposeTransiently expressing and visualizing the subcellular localization of TurboID fused with a nuclear export signal (NES) in plantaDepositorInsertNES-3XHA-TurboID
TagsGFPExpressionPlantMutationTurboID gene sequence C249APromoterCauliflower mosaic virus (CaMV) 35S promoterAvailable SinceJan. 5, 2024AvailabilityAcademic Institutions and Nonprofits only