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Enter a sequence to perform a BLAST-based search of the Addgene plasmid sequence database.

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We narrowed to 10,856 results for: kars;

Showing: 5281 - 5310 of 10856 results

  3. pFastBac1-OSF-TRIM5alpha(African green monkey pygerythrus)(1-293)

    Plasmid
    #79030
    Purpose
    Baculoviral entry vector to produce Bac-to-bac baculoviruses
    Depositor
    Wesley Sundquist
    Article
    Li et al Elife. 2016 Jun 2;5. pii: e16269. doi: 1
    Insert
    OSF-TRIM5alpha(African green monkey pygerythrus)(1-293)
    Use
    Tags
    OneSTrEP-FLAG
    Expression
    Insect
    Mutation
    deleted amino acid 294-515 from frican green monk…
    Promoter
    polyhedrin
    Available since
    Jan. 30, 2017
    Availability
    Academic Institutions and Nonprofits only
    79030-plasmid-map-sequence-id-147998

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  15. CXCR3-DuET

    Plasmid
    #213220
    Purpose
    The DuET construct encodes the named human GPCR engineered with a cleaved hemagglutinin signal peptide, a 5' FLAG and a 3' 1D4 epitope tag optimized for expression in mammalian cells and proteomics studies.
    Depositor
    Thomas Sakmar
    Article
    Dahl et al Sci Adv. 2023 May 3;9(18):eadf9297. doi:
    Insert
    CXCR3 (CXCR3 Human)
    Use
    Tags
    1D4 and FLAG
    Expression
    Mammalian
    Mutation
    Promoter
    CMV, T7
    Available since
    April 23, 2024
    Availability
    Academic Institutions and Nonprofits only
    213220-plasmid-map-sequence-id-430782

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  22. yeast_DualReporter

    Plasmid
    #127546
    Purpose
    A dual reporter vector modified from Sharon et al (Nature Biotech, 2012) containing Ura3 and Nat1 selectable markers, a constant background RFP (TEF2::mCherry), and a variable YFP (pTpA+MCS::yEVenus).
    Depositor
    Aviv Regev
    Article
    de Boer et al Nat Biotechnol. 2020 Jan;38(1):56-65. do
    Inserts
    bla
    mCherry
    URA3 (URA3 Budding Yeast)
    yEVenus
    Nat1
    Use
    Synthetic Biology
    Tags
    Expression
    Yeast
    Mutation
    Promoter
    TEF, TEF2, URA3, bla (E. coli), and pTpA+MCS
    Available since
    July 29, 2019
    Availability
    Academic Institutions and Nonprofits only
    127546-plasmid-map-sequence-id-251803

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  24. Lenti-EFS-BirAopt-GSQ-RBXN-P2A-BLAST

    Plasmid
    #208048
    Purpose
    Enables constitutive expression of BirAopt alone; RBXN represents a EcoR1-BsiW1-Xba1-Not1 polylinker; to perform bioE3; selection with blasticidin
    Depositor
    James Sutherland, Rosa Barrio
    Article
    Barroso-Gomila et al Nat Commun. 2023 Nov 23;14(1):7656. doi:
    Insert
    BirAopt
    Use
    Lentiviral
    Tags
    Expression
    Mutation
    BirAopt is human-codon-optimized BirA (PMID 15707…
    Promoter
    EFS
    Available since
    Nov. 29, 2023
    Availability
    Academic Institutions and Nonprofits only
    208048-plasmid-map-sequence-id-410202

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  26. 12xMoonTag-12xSunTag-kif18b-24xPP7

    Plasmid
    #128606
    Purpose
    Expresses 12xgp41 peptide array (MoonTag peptide array), followed by 12xgcn4 peptide array (SunTag peptide array), kif18b gene and 24 repeats of PP7 hairpins in 3’ UTR.
    Depositor
    Marvin Tanenbaum
    Article
    Boersma et al Cell. 2019 Jun 5. pii: S0092-8674(19)304
    Inserts
    12xMoonTag-12xSunTag
    kinesin family member 18b (KIF18B Human)
    Use
    Tags
    Expression
    Mammalian
    Mutation
    Promoter
    Pcmv
    Available since
    Aug. 14, 2019
    Availability
    Academic Institutions and Nonprofits only
    128606-plasmid-map-sequence-id-254156

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  28. TDP-REGv2(mScarlet-FLAG) matched positive control in pTwist-CMV

    Plasmid
    #216156
    Purpose
    Expresses mScarlet regardless of TDP-43 knockdown. Note: It is not recommended to produce lentiviruses containing TDP-REG sequences – see Depositor Comments
    Depositor
    Pietro Fratta
    Article
    Wilkins et al Science. 2024 Oct 4;386(6717):61-69. doi
    Insert
    mScarlet with C-terminal FLAG tag
    Use
    Tags
    Expression
    Mammalian
    Mutation
    Promoter
    Available since
    July 1, 2024
    Availability
    Academic Institutions and Nonprofits only
    216156-plasmid-map-sequence-id-437768

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  29. EF1a_MYC deltaCTD_P2A_Hygro_Barcode

    Plasmid
    #120511
    Purpose
    Barcoded lentiviral vector to express MYC deltaCTD in mammalian cells under control of EF1a promoter. Barcoded for transcript detection in single cell RNA-seq.
    Depositor
    Prashant Mali
    Article
    Parekh et al Cell Syst. 2018 Nov 12. pii: S2405-4712(
    Insert
    MYC deltaCTD (MYC Human)
    Use
    Lentiviral
    Tags
    Expression
    Mutation
    Deletion of carboxy-terminal domain
    Promoter
    EF1a
    Available since
    Feb. 7, 2019
    Availability
    Academic Institutions and Nonprofits only
    120511-plasmid-map-sequence-id-234934

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  30. EF1a_MYC deltaNTD_P2A_Hygro_Barcode

    Plasmid
    #120510
    Purpose
    Barcoded lentiviral vector to express MYC deltaNTD in mammalian cells under control of EF1a promoter. Barcoded for transcript detection in single cell RNA-seq.
    Depositor
    Prashant Mali
    Article
    Parekh et al Cell Syst. 2018 Nov 12. pii: S2405-4712(
    Insert
    MYC deltaNTD (MYC Human)
    Use
    Lentiviral
    Tags
    Expression
    Mutation
    Deletion of amino-terminal domain
    Promoter
    EF1a
    Available since
    Feb. 7, 2019
    Availability
    Academic Institutions and Nonprofits only
    120510-plasmid-map-sequence-id-234933

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Showing: 5281 - 5310 of 10856 results