We narrowed to 11,255 results for: NSI

1. pET28a_6HT-TBP6.7(R47A)

   Plasmid

   #112722

   Purpose

   Arg47 within TBP6.7 is the most critical residue in terms of forming interactions with WT TAR. Mutating this amino acid to Ala results in ~600-fold reduction in Kd.

   Depositor

   Joseph Wedekind

   Article

   Belashov et al Nucleic Acids Res. 2018 Jul 27;46(13):64

   Insert

   6His-TEV-TBP6.7(R47A)
   

   Use

   Tags

   6His-TEV

   Expression

   Bacterial

   Mutation

   R47A

   Promoter

   T7

   Available since

   Aug. 8, 2018

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

2. pET28a_6HT-TBP6.7(R47K)

   Plasmid

   #112723

   Purpose

   Arg47 of TBP6.7 interacts with Hoogsteen edge of TAR's Gua26. Mutating this amino acid to Lys reduces binding to the WT TAR RNA ~330-fold.

   Depositor

   Joseph Wedekind

   Article

   Belashov et al Nucleic Acids Res. 2018 Jul 27;46(13):64

   Insert

   6His-TEV-TBP6.7(R47K)
   

   Use

   Tags

   6His-TEV

   Expression

   Bacterial

   Mutation

   R47K

   Promoter

   T7

   Available since

   Aug. 8, 2018

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

3. pET28a_6HT-TBP6.7(Q48A)

   Plasmid

   #112724

   Purpose

   Gln48 contacts the backbone of TAR RNA as well as mediates intramoecular interaction to stabilize the conformation of beta2-beta3 loop within TBP6.7.

   Depositor

   Joseph Wedekind

   Article

   Belashov et al Nucleic Acids Res. 2018 Jul 27;46(13):64

   Insert

   6His-TEV-TBP6.7(Q48A)
   

   Use

   Tags

   6His-TEV

   Expression

   Bacterial

   Mutation

   Q48A

   Promoter

   T7

   Available since

   Aug. 8, 2018

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

4. pET28a_6HT-TBP6.7(T50A)

   Plasmid

   #112727

   Purpose

   Lav-evolved amino acid T50 within TBPs is important in engaging intramolecularly to steer also lab-evolved R49 into conformation compatible binding to RNA.

   Depositor

   Joseph Wedekind

   Article

   Belashov et al Nucleic Acids Res. 2018 Jul 27;46(13):64

   Insert

   6His-TEV-TBP6.7(T50A)
   

   Use

   Tags

   6His-TEV

   Expression

   Bacterial

   Mutation

   T50A

   Promoter

   T7

   Available since

   Aug. 8, 2018

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

5. pET28a_6HT-TBP6.7(R52A)

   Plasmid

   #112729

   Purpose

   52nd position is Arg in both wild-type U1A and TBPs, but the RNA recognition by this residue in both types of proteins is fundamentally different.

   Depositor

   Joseph Wedekind

   Article

   Belashov et al Nucleic Acids Res. 2018 Jul 27;46(13):64

   Insert

   6His-TEV-TBP6.7(R52A)
   

   Use

   Tags

   6His-TEV

   Expression

   Bacterial

   Mutation

   R52A

   Promoter

   T7

   Available since

   Aug. 8, 2018

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

6. pET28a_6HT-TBP6.7(delta C-term)

   Plasmid

   #112731

   Purpose

   This mutant of TBP6.7 is lacking residues 91-98 at C-terminus, and was used to test the involvement of those residues in TAR recognition.

   Depositor

   Joseph Wedekind

   Article

   Belashov et al Nucleic Acids Res. 2018 Jul 27;46(13):64

   Insert

   6His-TEV-TBP6.7(delta C-term)
   

   Use

   Tags

   6His-TEV

   Expression

   Bacterial

   Mutation

   delta C-term

   Promoter

   T7

   Available since

   Aug. 8, 2018

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

7. pGEX6P-1 PUM FL

   Plasmid

   #112997

   Purpose

   For protein expression and purification of Drosophila pumilio (amino acids 566-1090)

   Depositor

   Terry Orr-Weaver

   Article

   Hara et al Elife. 2018 Feb 26;7. pii: 33150. doi: 1

   Insert

   pumilio (pum Fly)
   

   Use

   Tags

   Expression

   Bacterial

   Mutation

   Promoter

   Available since

   Aug. 3, 2018

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

8. pET-30a(+)-N-SH2+IA

   Plasmid

   #111273

   Purpose

   express murine Syk (N)SH2 domain plus interdomain A (Ser 8 to His 162), with an N-terminal (His)6 tag and TEV cleavage site, in E.coli strain Rosetta 2 (DE3)

   Depositor

   Carol Post

   Article

   Feng et al Phys Chem Chem Phys. 2016 Feb 17;18(8):5

   Insert

   murine Syk (N)SH2 domain plus interdomain A (Ser 8 to His 162), with an N-terminal (His)6 tag and TEV cleavage site (Syk Mouse)
   

   Use

   Tags

   HHHHHHENLYFQG

   Expression

   Bacterial

   Mutation

   Promoter

   T7 promoter

   Available since

   July 17, 2018

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

9. pEGFP-vsv-Mis12-dCEN-Incenp-EGFP

   Plasmid

   #108478

   Purpose

   expression of vsv-Mis12-dCEN-INCENP-EGFP

   Depositor

   Susanne Lens

   Article

   Liu et al Science. 2009 Mar 6;323(5919):1350-3. do

   Insert

   Mis12-dCEN-INCENP (INCENP Human)
   

   Use

   Tags

   EGFP and VSV

   Expression

   Mammalian

   Mutation

   CEN box replaced by Mis12

   Promoter

   CMV

   Available since

   May 8, 2018

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

10. pHH0103_FNBP1-1/1

    Plasmid

    #91552

    Purpose

    Protein expression and purification of human SH3 domain construct FNBP1-1/1

    Depositor

    Sachdev Sidhu

    Article

    Teyra et al Structure. 2017 Oct 3;25(10):1598-1610.e

    Insert

    FNBP1-1/1 (FNBP1 Human)
    

    Use

    Tags

    Expression

    Bacterial

    Mutation

    Promoter

    Available since

    March 19, 2018

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

11. pHH0103_ABL2-1/1

    Plasmid

    #91533

    Purpose

    Protein expression and purification of human SH3 domain construct ABL2-1/1

    Depositor

    Sachdev Sidhu

    Article

    Teyra et al Structure. 2017 Oct 3;25(10):1598-1610.e

    Insert

    ABL2-1/1 (ABL2 Human)
    

    Use

    Tags

    Expression

    Bacterial

    Mutation

    Promoter

    Available since

    March 19, 2018

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

12. pHH0103_CACNB2-2/2

    Plasmid

    #91507

    Purpose

    Protein expression and purification of human SH3 domain construct CACNB2-2/2

    Depositor

    Sachdev Sidhu

    Article

    Teyra et al Structure. 2017 Oct 3;25(10):1598-1610.e

    Insert

    CACNB2-2/2 (CACNB2 Human)
    

    Use

    Tags

    Expression

    Bacterial

    Mutation

    Promoter

    Available since

    March 19, 2018

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

13. pHH0103_CD2AP-3/3

    Plasmid

    #91508

    Purpose

    Protein expression and purification of human SH3 domain construct CD2AP-3/3

    Depositor

    Sachdev Sidhu

    Article

    Teyra et al Structure. 2017 Oct 3;25(10):1598-1610.e

    Insert

    CD2AP-3/3 (CD2AP Human)
    

    Use

    Tags

    Expression

    Bacterial

    Mutation

    Promoter

    Available since

    March 19, 2018

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

14. pHH0103_DLG5-1/1

    Plasmid

    #91522

    Purpose

    Protein expression and purification of human SH3 domain construct DLG5-1/1

    Depositor

    Sachdev Sidhu

    Article

    Teyra et al Structure. 2017 Oct 3;25(10):1598-1610.e

    Insert

    DLG5-1/1 (DLG5 Human)
    

    Use

    Tags

    Expression

    Bacterial

    Mutation

    Promoter

    Available since

    March 19, 2018

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

15. pHH0103_CASKIN1-1/1

    Plasmid

    #91523

    Purpose

    Protein expression and purification of human SH3 domain construct CASKIN1-1/1

    Depositor

    Sachdev Sidhu

    Article

    Teyra et al Structure. 2017 Oct 3;25(10):1598-1610.e

    Insert

    CASKIN1-1/1 (CASKIN1 Human)
    

    Use

    Tags

    Expression

    Bacterial

    Mutation

    Promoter

    Available since

    March 19, 2018

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

16. pHH0103_ARHGAP26-1/1

    Plasmid

    #91484

    Purpose

    Protein expression and purification of human SH3 domain construct ARHGAP26-1/1

    Depositor

    Sachdev Sidhu

    Article

    Teyra et al Structure. 2017 Oct 3;25(10):1598-1610.e

    Insert

    ARHGAP26-1/1 (ARHGAP26 Human)
    

    Use

    Tags

    Expression

    Bacterial

    Mutation

    Promoter

    Available since

    March 19, 2018

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

17. pHH0103_ITSN2-4/5

    Plasmid

    #91496

    Purpose

    Protein expression and purification of human SH3 domain construct ITSN2-4/5

    Depositor

    Sachdev Sidhu

    Article

    Teyra et al Structure. 2017 Oct 3;25(10):1598-1610.e

    Insert

    ITSN2-4/5 (ITSN2 Human)
    

    Use

    Tags

    Expression

    Bacterial

    Mutation

    Promoter

    Available since

    March 19, 2018

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

18. pHH0103_ITSN2-1/5

    Plasmid

    #91497

    Purpose

    Protein expression and purification of human SH3 domain construct ITSN2-1/5

    Depositor

    Sachdev Sidhu

    Article

    Teyra et al Structure. 2017 Oct 3;25(10):1598-1610.e

    Insert

    ITSN2-1/5 (ITSN2 Human)
    

    Use

    Tags

    Expression

    Bacterial

    Mutation

    Promoter

    Available since

    March 19, 2018

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

19. pHH0103_EPS8L1-1/1-IS1/2

    Plasmid

    #91465

    Purpose

    Protein expression and purification of human SH3 domain construct EPS8L1-1/1-IS1/2

    Depositor

    Sachdev Sidhu

    Article

    Teyra et al Structure. 2017 Oct 3;25(10):1598-1610.e

    Insert

    EPS8L1-1/1-IS1/2 (EPS8L1 Human)
    

    Use

    Tags

    Expression

    Bacterial

    Mutation

    Promoter

    Available since

    March 19, 2018

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

20. pHH0103_ITSN2-3/5

    Plasmid

    #91470

    Purpose

    Protein expression and purification of human SH3 domain construct ITSN2-3/5

    Depositor

    Sachdev Sidhu

    Article

    Teyra et al Structure. 2017 Oct 3;25(10):1598-1610.e

    Insert

    ITSN2-3/5 (ITSN2 Human)
    

    Use

    Tags

    Expression

    Bacterial

    Mutation

    Promoter

    Available since

    March 19, 2018

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

21. pHH0103_FCHSD2-1/2

    Plasmid

    #91444

    Purpose

    Protein expression and purification of human SH3 domain construct FCHSD2-1/2

    Depositor

    Sachdev Sidhu

    Article

    Teyra et al Structure. 2017 Oct 3;25(10):1598-1610.e

    Insert

    FCHSD2-1/2 (FCHSD2 Human)
    

    Use

    Tags

    Expression

    Bacterial

    Mutation

    Promoter

    Available since

    March 19, 2018

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

22. pHH0103_DLG1-1/1

    Plasmid

    #91448

    Purpose

    Protein expression and purification of human SH3 domain construct DLG1-1/1

    Depositor

    Sachdev Sidhu

    Article

    Teyra et al Structure. 2017 Oct 3;25(10):1598-1610.e

    Insert

    DLG1-1/1 (DLG1 Human)
    

    Use

    Tags

    Expression

    Bacterial

    Mutation

    Promoter

    Available since

    March 19, 2018

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

23. pHH0103_FCHSD1-1/2

    Plasmid

    #91449

    Purpose

    Protein expression and purification of human SH3 domain construct FCHSD1-1/2

    Depositor

    Sachdev Sidhu

    Article

    Teyra et al Structure. 2017 Oct 3;25(10):1598-1610.e

    Insert

    FCHSD1-1/2 (FCHSD1 Human)
    

    Use

    Tags

    Expression

    Bacterial

    Mutation

    Promoter

    Available since

    March 19, 2018

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

24. pHH0103_ARHGEF5-1/1

    Plasmid

    #91452

    Purpose

    Protein expression and purification of human SH3 domain construct ARHGEF5-1/1

    Depositor

    Sachdev Sidhu

    Article

    Teyra et al Structure. 2017 Oct 3;25(10):1598-1610.e

    Insert

    ARHGEF5-1/1 (ARHGEF5 Human)
    

    Use

    Tags

    Expression

    Bacterial

    Mutation

    Promoter

    Available since

    March 19, 2018

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

25. pHH0103_ITSN2-5/5

    Plasmid

    #91454

    Purpose

    Protein expression and purification of human SH3 domain construct ITSN2-5/5

    Depositor

    Sachdev Sidhu

    Article

    Teyra et al Structure. 2017 Oct 3;25(10):1598-1610.e

    Insert

    ITSN2-5/5 (ITSN2 Human)
    

    Use

    Tags

    Expression

    Bacterial

    Mutation

    Promoter

    Available since

    March 19, 2018

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

26. pHH0103_EFS-1/1

    Plasmid

    #91456

    Purpose

    Protein expression and purification of human SH3 domain construct EFS-1/1

    Depositor

    Sachdev Sidhu

    Article

    Teyra et al Structure. 2017 Oct 3;25(10):1598-1610.e

    Insert

    EFS-1/1 (EFS Human)
    

    Use

    Tags

    Expression

    Bacterial

    Mutation

    Promoter

    Available since

    March 19, 2018

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

27. pHH0103_FCHSD2-2/2

    Plasmid

    #91460

    Purpose

    Protein expression and purification of human SH3 domain construct FCHSD2-2/2

    Depositor

    Sachdev Sidhu

    Article

    Teyra et al Structure. 2017 Oct 3;25(10):1598-1610.e

    Insert

    FCHSD2-2/2 (FCHSD2 Human)
    

    Use

    Tags

    Expression

    Bacterial

    Mutation

    Promoter

    Available since

    March 19, 2018

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

28. pHH0103_ARHGEF19-1/1

    Plasmid

    #91432

    Purpose

    Protein expression and purification of human SH3 domain construct ARHGEF19-1/1

    Depositor

    Sachdev Sidhu

    Article

    Teyra et al Structure. 2017 Oct 3;25(10):1598-1610.e

    Insert

    ARHGEF19-1/1 (ARHGEF19 Human)
    

    Use

    Tags

    Expression

    Bacterial

    Mutation

    Promoter

    Available since

    March 19, 2018

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

29. pHH0103_FNBP1L-1/1

    Plasmid

    #91435

    Purpose

    Protein expression and purification of human SH3 domain construct FNBP1L-1/1

    Depositor

    Sachdev Sidhu

    Article

    Teyra et al Structure. 2017 Oct 3;25(10):1598-1610.e

    Insert

    FNBP1L-1/1 (FNBP1L Human)
    

    Use

    Tags

    Expression

    Bacterial

    Mutation

    Promoter

    Available since

    March 19, 2018

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

30. pHH0103_EPS8L3-1/1

    Plasmid

    #91395

    Purpose

    Protein expression and purification of human SH3 domain construct EPS8L3-1/1

    Depositor

    Sachdev Sidhu

    Article

    Teyra et al Structure. 2017 Oct 3;25(10):1598-1610.e

    Insert

    EPS8L3-1/1 (EPS8L3 Human)
    

    Use

    Tags

    Expression

    Bacterial

    Mutation

    Promoter

    Available since

    March 19, 2018

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

31. pHH0103_MACC1-1/1

    Plasmid

    #91360

    Purpose

    Protein expression and purification of human SH3 domain construct MACC1-1/1

    Depositor

    Sachdev Sidhu

    Article

    Teyra et al Structure. 2017 Oct 3;25(10):1598-1610.e

    Insert

    MACC1-1/1 (MACC1 Human)
    

    Use

    Tags

    Expression

    Bacterial

    Mutation

    Promoter

    Available since

    March 19, 2018

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

32. pHH0103_ARHGEF38-1/1

    Plasmid

    #91373

    Purpose

    Protein expression and purification of human SH3 domain construct ARHGEF38-1/1

    Depositor

    Sachdev Sidhu

    Article

    Teyra et al Structure. 2017 Oct 3;25(10):1598-1610.e

    Insert

    ARHGEF38-1/1 (ARHGEF38 Human)
    

    Use

    Tags

    Expression

    Bacterial

    Mutation

    Promoter

    Available since

    March 19, 2018

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

33. pHH0103_MACC1-1/1

    Plasmid

    #91333

    Purpose

    Protein expression and purification of human SH3 domain construct MACC1-1/1

    Depositor

    Sachdev Sidhu

    Article

    Teyra et al Structure. 2017 Oct 3;25(10):1598-1610.e

    Insert

    MACC1-1/1 (MACC1 Human)
    

    Use

    Tags

    Expression

    Bacterial

    Mutation

    Promoter

    Available since

    March 19, 2018

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

34. pHH0103_ARHGAP33-1/1

    Plasmid

    #91341

    Purpose

    Protein expression and purification of human SH3 domain construct ARHGAP33-1/1

    Depositor

    Sachdev Sidhu

    Article

    Teyra et al Structure. 2017 Oct 3;25(10):1598-1610.e

    Insert

    ARHGAP33-1/1 (ARHGAP33 Human)
    

    Use

    Tags

    Expression

    Bacterial

    Mutation

    Promoter

    Available since

    March 19, 2018

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

35. pHH0103_MAP3K10-1/1

    Plasmid

    #91329

    Purpose

    Protein expression and purification of human SH3 domain construct MAP3K10-1/1

    Depositor

    Sachdev Sidhu

    Article

    Teyra et al Structure. 2017 Oct 3;25(10):1598-1610.e

    Insert

    MAP3K10-1/1 (MAP3K10 Human)
    

    Use

    Tags

    Expression

    Bacterial

    Mutation

    Promoter

    Available since

    March 19, 2018

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

36. pHH0103_ARHGEF37-1/2

    Plasmid

    #91310

    Purpose

    Protein expression and purification of human SH3 domain construct ARHGEF37-1/2

    Depositor

    Sachdev Sidhu

    Article

    Teyra et al Structure. 2017 Oct 3;25(10):1598-1610.e

    Insert

    ARHGEF37-1/2 (ARHGEF37 Human)
    

    Use

    Tags

    Expression

    Bacterial

    Mutation

    Promoter

    Available since

    March 19, 2018

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

37. pHH0103_ARHGEF37-2/2

    Plasmid

    #91311

    Purpose

    Protein expression and purification of human SH3 domain construct ARHGEF37-2/2

    Depositor

    Sachdev Sidhu

    Article

    Teyra et al Structure. 2017 Oct 3;25(10):1598-1610.e

    Insert

    ARHGEF37-2/2 (ARHGEF37 Human)
    

    Use

    Tags

    Expression

    Bacterial

    Mutation

    Promoter

    Available since

    March 19, 2018

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

38. pHH0103_CACNB2-1/2

    Plasmid

    #91314

    Purpose

    Protein expression and purification of human SH3 domain construct CACNB2-1/2

    Depositor

    Sachdev Sidhu

    Article

    Teyra et al Structure. 2017 Oct 3;25(10):1598-1610.e

    Insert

    CACNB2-1/2 (CACNB2 Human)
    

    Use

    Tags

    Expression

    Bacterial

    Mutation

    Promoter

    Available since

    March 19, 2018

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

39. pHH0103_EPS8L1-1/1-IS2/2

    Plasmid

    #91315

    Purpose

    Protein expression and purification of human SH3 domain construct EPS8L1-1/1-IS2/2

    Depositor

    Sachdev Sidhu

    Article

    Teyra et al Structure. 2017 Oct 3;25(10):1598-1610.e

    Insert

    EPS8L1-1/1-IS2/2 (EPS8L1 Human)
    

    Use

    Tags

    Expression

    Bacterial

    Mutation

    Promoter

    Available since

    March 19, 2018

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

40. pHH0103_FCHSD1-2/2

    Plasmid

    #91258

    Purpose

    Protein expression and purification of human SH3 domain construct FCHSD1-2/2

    Depositor

    Sachdev Sidhu

    Article

    Teyra et al Structure. 2017 Oct 3;25(10):1598-1610.e

    Insert

    FCHSD1-2/2 (FCHSD1 Human)
    

    Use

    Tags

    Expression

    Bacterial

    Mutation

    Promoter

    Available since

    March 19, 2018

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge