We narrowed to 31,602 results for: REP

Showing: 3541 - 3560 of 31602 results

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AAV-GFAP(long)-FLEX-GCaMP7f

Plasmid

#189612

Purpose

Cre-dependent GCaMP7f under the GFAP promoter

Depositor

Martin Riccomagno

Article

Agnew-Svoboda et al Cell Rep Methods. 2022 Aug 22;2(8):10027

Insert

GCaMP7f

Use

AAV and Cre/Lox

Tags

GCaMP7f

Expression

Mammalian

Available Since

Sept. 16, 2022

Availability

Academic Institutions and Nonprofits only

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pX330-UGCG-KO

Plasmid

#80010

Purpose

gRNA to knock out expression of UGCG gene. The product of this gene catalyzes the first step in synthesis of glycosphingolipids.

Depositor

Sriram Neelamegham

Article

Stolfa et al Sci Rep. 2016 Jul 26;6:30392. doi: 10.10

Insert

UGCG (UGCG Human)

Use

CRISPR

Available Since

July 25, 2016

Availability

Academic Institutions and Nonprofits only

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MBD_R44Q-IFP2.0C

Plasmid

#215749

Purpose

Expresses binding-deficient R44Q mutant of MBD1 MBD domain fused to C-terminal part of IFP2.0 as negative control for 5mC detection in BiAD sensors

Depositor

Albert Jeltsch

Article

Kohler et al Cell Rep Methods. 2024 Mar 26:100739. do

Insert

IFP2.0C

Expression

Mammalian

Promoter

CMV

Available Since

April 16, 2024

Availability

Academic Institutions and Nonprofits only

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2xHP1bCD-IFP2.0C

Plasmid

#215750

Purpose

Expresses double domain of HP1beta chromo domain fused to C-terminal part of IFP2.0 for H3K9me2/3 detection in BiAD sensors

Depositor

Albert Jeltsch

Article

Kohler et al Cell Rep Methods. 2024 Mar 26:100739. do

Insert

2xHP1bCD-IFP2.0C

Expression

Mammalian

Promoter

CMV

Available Since

April 16, 2024

Availability

Academic Institutions and Nonprofits only

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MacroH2A.2-c002

Plasmid

#173089

Purpose

Expression of recombinant fusion protein in E. coli

Depositor

Lari Lehtiö

Article

Sowa et al Cell Rep Methods. 2021 Dec 20;1(8):10012

Insert

MACROH2A2 (MACROH2A2 Human)

Tags

mCerulean

Expression

Bacterial

Mutation

K167-K372

Available Since

Jan. 13, 2022

Availability

Academic Institutions and Nonprofits only

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pUC Ad VAI

Plasmid

#175518

Purpose

For mammalian expression of adenovirus VAI RNA

Depositor

Britt Glaunsinger

Article

Mendez et al Cell Rep. 2021 Oct 19;37(3):109841. doi:

Insert

Ad VAI RNA

Use

Adenoviral

Promoter

lac promoter

Available Since

Oct. 8, 2021

Availability

Academic Institutions and Nonprofits only

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ALC1-c005

Plasmid

#173092

Purpose

Expression of recombinant fusion protein in E. coli

Depositor

Lari Lehtiö

Article

Sowa et al Cell Rep Methods. 2021 Dec 20;1(8):10012

Insert

CHD1L (CHD1L Human)

Tags

mCerulean

Expression

Bacterial

Mutation

L613-K879, codon optimized

Available Since

Jan. 13, 2022

Availability

Academic Institutions and Nonprofits only

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LAMP1-ABKAR

Plasmid

#65068

Purpose

Expression of ABKAR biosensor targeted to the lysozome

Depositor

Takanari Inoue, Jin Zhang

Article

Miyamoto et al Cell Rep. 2015 Apr 28;11(4):657-70. doi:

Insert

ABKAR biosensor

Tags

Lamp1

Expression

Mammalian

Promoter

CMV

Available Since

June 11, 2015

Availability

Academic Institutions and Nonprofits only
Nuc-ABKAR

Plasmid

#61511

Purpose

Expression of nuclear AMPK biosensor

Depositor

Takanari Inoue, Jin Zhang

Article

Miyamoto et al Cell Rep. 2015 Apr 28;11(4):657-70. doi:

Insert

AMPK Biosensor

Tags

NLS (see comments)

Expression

Mammalian

Promoter

CMV

Available Since

April 17, 2015

Availability

Academic Institutions and Nonprofits only
pCSIIbsr-6013x

Plasmid

#108654

Purpose

An HRas biosensor based on fluorescence resonance energy transfer (FRET) and those based on bioluminescence energy transfer (BRET)

Depositor

Michiyuki Matsuda

Article

Komatsu et al Sci Rep. 2018 Jun 12;8(1):8984. doi: 10.

Insert

Raichu-EV

Use

Lentiviral

Tags

XFP

Expression

Mammalian

Available Since

Aug. 20, 2018

Availability

Academic Institutions and Nonprofits only

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CCR5-donor-MCS

Plasmid

#192269

Purpose

Donor template for CRISPR Genome Editing

Depositor

Shantanu Chowdhury

Article

Sharma et al Cell Rep. 2021 May 18;35(7):109154. doi:

Insert

MCS within donor template

Use

CRISPR

Available Since

Dec. 20, 2022

Availability

Academic Institutions and Nonprofits only

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Golgi-ABKAR

Plasmid

#61507

Purpose

Expression of AMPK biosensor targeted to the Golgi

Depositor

Takanari Inoue, Jin Zhang

Article

Miyamoto et al Cell Rep. 2015 Apr 28;11(4):657-70. doi:

Insert

AMPK Biosensor

Tags

Golgi-targeting signal (see comments)

Expression

Mammalian

Promoter

CMV

Available Since

April 17, 2015

Availability

Academic Institutions and Nonprofits only
Tensin-2 WT

Plasmid

#201797

Purpose

Expresses wild-type Tensin-2 protein in MAC-Tag-C backbone

Depositor

Vesa Hytönen

Article

Turkki et al Sci Rep. 2024 Aug 27;14(1):19862. doi: 1

Insert

Tensin-2 (Tns2 Mouse)

Tags

BirA, HA, strepIII

Expression

Bacterial and Mammalian

Available Since

Dec. 11, 2024

Availability

Academic Institutions and Nonprofits only

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pBS-KLF7-200-GFPfr1

Plasmid

#169799

Purpose

THe donor vector for the KLF7 gene locus.

Depositor

Natsuko Chiba

Article

Yoshino et al Sci Rep. 2019 Feb 7;9(1):1644. doi: 10.1

Insert

Donor sequence to KLF7 neighbouring region for HR

Use

Hr donor vector for human actb gene.

Mutation

Partial sequence of GFP is inserted the sequence …

Available Since

Aug. 4, 2021

Availability

Academic Institutions and Nonprofits only

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LentiCRISPRv2-KLF7-1

Plasmid

#169797

Purpose

Expresses Cas9 and guide RNA for the site neighbouring KLF7 gene.

Depositor

Natsuko Chiba

Article

Yoshino et al Sci Rep. 2019 Feb 7;9(1):1644. doi: 10.1

Insert

guide RNA for KLF7 neighbouring region

Use

CRISPR and Lentiviral

Expression

Mammalian

Promoter

U6

Available Since

June 11, 2021

Availability

Academic Institutions and Nonprofits only

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pMSCV-puro-TTF-1-wt

Plasmid

#74990

Purpose

Retroviral vector holding the human wild-type full-length TTF-1 (NKX2-1) cDNA

Depositor

David Mu

Article

Wood et al Sci Rep. 2016 Feb 25;6:19857. doi: 10.10

Insert

TTF-1 (TTF1 Human)

Use

Retroviral

Expression

Mammalian

Mutation

wild-type, full-length, without 3' UTR

Available Since

May 26, 2016

Availability

Academic Institutions and Nonprofits only

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pMK285 (Stag-3FLAG-Bsr)

Plasmid

#72801

Purpose

C-terminal tagging with Stag-3FLAG using CRIPSR/Cas9

Depositor

Masato Kanemaki

Article

Natsume et al Cell Rep. 2016 Apr 5;15(1):210-8. doi: 1

Insert

Stag-3FLAG-Bsr

Use

CRISPR

Tags

Stag-3FLAG

Expression

Mammalian

Available Since

March 24, 2016

Availability

Academic Institutions and Nonprofits only

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ARH3-c002

Plasmid

#173095

Purpose

Expression of recombinant fusion protein in E. coli

Depositor

Lari Lehtiö

Article

Sowa et al Cell Rep Methods. 2021 Dec 20;1(8):10012

Insert

ADPRS (ADPRS Human)

Tags

mCerulean

Expression

Bacterial

Mutation

A14-S363

Available Since

Dec. 9, 2021

Availability

Academic Institutions and Nonprofits only

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pJRH056

Plasmid

#171637

Purpose

Plasmid containing a U6 promoter expressing a spyCas9 tdTom sgRNA298 and CAG promoter expressing mTagBFP2

Depositor

Jennifer Doudna

Article

Hamilton et al Cell Rep. 2021 Jun 1;35(9):109207. doi:

Inserts

spyCas9 sgRNA-tdTomato
mTagBFP2

Use

CRISPR and Lentiviral

Expression

Mammalian

Promoter

CAG and U6

Available Since

June 30, 2021

Availability

Academic Institutions and Nonprofits only

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pMito-Electra1

Plasmid

#179481

Purpose

Mitochondrial labeling with blue fluorescent protein Electra1 fused to partial COX8A

Depositor

Kiryl Piatkevich

Article

Papadaki et al Sci Rep. 2022 Jun 17;12(1):10190. doi: 1

Insert

COX8A-Electra1

Tags

Electra1

Expression

Mammalian

Promoter

CMV

Available Since

Aug. 1, 2022

Availability

Academic Institutions and Nonprofits only

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