We narrowed to 13,169 results for: Mpl;
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Plasmid#89145PurposeExpression of helicase mutant of human Dicer in Sf9 cellsDepositorInserthDicer (DICER1 Human)
UseTagsHis6-tevExpressionInsectMutationK70APromoterAvailable sinceApril 25, 2017AvailabilityAcademic Institutions and Nonprofits only -
P21-sp64 Poly A
Plasmid#59433Purposesynthetic poly A transcripts of VZVDepositorInsertVZV ORF 21
UseMakes synthetic transcripts that contains poly aTagsnoneExpressionMutationnonePromoterSP6Available sinceSept. 2, 2014AvailabilityAcademic Institutions and Nonprofits only -
HLH Bmal Pas 1-465-VenC
Plasmid#47364PurposeBmal fragment cloned with Venus tag and used for BiFCDepositorInsertBMAL (Bmal1 Mouse)
UseTagsVenus aa 156-239ExpressionMammalianMutationaa 1-465PromoterCMVAvailable sinceJan. 7, 2014AvailabilityAcademic Institutions and Nonprofits only -
FHpHG_mTent5c_LxxAA (L3m)
Plasmid#221441PurposeTransfection into the cells for the reporter or qPCR assay.DepositorInsertTent5c_LXXAA (L3m) (Fam46c Mouse)
UseTagsFLAG-2xHA-(HRV 3C site)-6xHis-EGFPExpressionMammalianMutationL332A, LL335-336AA (CTC atc tct CTC CTG to CTC at…PromoterAvailable sinceJune 12, 2025AvailabilityAcademic Institutions and Nonprofits only -
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pETM11-6xHis-TEV-hORF1p
Plasmid#202589PurposeAllows for IPTG-inducible expression of the human L1RP ORF1 protein in bacteria with an N-terminal 6xHis tag and TEV cleavage site for purification with a minimal scarDepositorInsertORF1p
UseTags6x His and TEV protease cleavage siteExpressionBacterialMutationPromoterAvailable sinceJune 4, 2025AvailabilityAcademic Institutions and Nonprofits only -
pcDNA4c/His-Xpress-HA-hBIG2(WT)
Plasmid#79432PurposeExpresses N-terminally His6, Xpress and HA-tagged BIG2(WT) in mammalian cellsDepositorInsertBIG2 (ARFGEF2 Human)
UseTagsHA, His6, and XpressExpressionMammalianMutationPromoterAvailable sinceJune 2, 2025AvailabilityAcademic Institutions and Nonprofits only -
pcDNA4c/His-Xpress-HA-hBIG1(WT)
Plasmid#79431PurposeExpresses N-terminally His6, Xpress and HA-tagged BIG1(WT) in mammalian cellsDepositorInsertBIG1 (ARFGEF1 Human)
UseTagsHA, His6, and XpressExpressionMammalianMutationC1758RPromoterAvailable sinceJune 2, 2025AvailabilityAcademic Institutions and Nonprofits only -
pUC18 3xFLAG COP1
Plasmid#237334Purposeexpression in bacterial cells for protein purificationDepositorInsertCOP1 (COP1 Human)
UseTagsExpressionBacterialMutationPromoterAvailable sinceMay 19, 2025AvailabilityAcademic Institutions and Nonprofits only -
pSMPPv2-Tau-RING (M72E/I18R)
Plasmid#233690PurposeExpression of Tau-RING I18R-M72E mutant in mammalian cellsDepositorInsertTau protein fused to TRIM21 RING domain carrying M72E/I18R mutations
UseLentiviralTagsExpressionMammalianMutationPromoterAvailable sinceApril 18, 2025AvailabilityAcademic Institutions and Nonprofits only -
pOPTG-Tau-RING (M72E/I18R)
Plasmid#233692PurposeExpression of Tau-RING I18R-M72E mutant in bacterial cellsDepositorInsertTau protein fused to TRIM21 RING domain
UseTagsExpressionBacterialMutationPromoterAvailable sinceApril 18, 2025AvailabilityAcademic Institutions and Nonprofits only -
pRDA355_sgCiPOLR2D
Plasmid#229022PurposeExpression of a CRISPRi doxycycline inducible guide targeting POLR2DDepositorInsertPOLR2D gRNA
UseCRISPR and LentiviralTagsExpressionMammalianMutationPromoterAvailable sinceApril 9, 2025AvailabilityAcademic Institutions and Nonprofits only -
OPEN-HLA-A*11:01-BirA
Plasmid#235135PurposeE. coli expression of BirA tagged HLA-A*11:01 containing a cysteine mutation for disulfide linkage to mutant beta-2 microglobulin.DepositorInsertOPEN-HLA-A*11:01 (HLA-A Human)
UseTagsBirAExpressionBacterialMutationG120CPromoterAvailable sinceApril 8, 2025AvailabilityAcademic Institutions and Nonprofits only -
pRDA355_sgCiPELO #2
Plasmid#229020PurposeExpression of a CRISPRi doxycycline inducible guide targeting PELODepositorInsertPELO gRNA
UseCRISPR and LentiviralTagsExpressionMammalianMutationPromoterAvailable sinceApril 3, 2025AvailabilityAcademic Institutions and Nonprofits only -
pRDA052H_sgCh2
Plasmid#229023PurposeExpression of a EnAsCas12a control guide that cuts an intergenic region on chromosome 2DepositorInsertsgChr2
UseCRISPR and LentiviralTagsExpressionMammalianMutationPromoterAvailable sinceApril 3, 2025AvailabilityAcademic Institutions and Nonprofits only -
pRDA_174_neoR
Plasmid#228935PurposeLentiviral expression of EnAsCas12aDepositorTypeEmpty backboneUseCRISPR and LentiviralTagsExpressionMammalianMutationPromoterAvailable sinceApril 3, 2025AvailabilityAcademic Institutions and Nonprofits only -
pRDA052H_sgFOCAD #2
Plasmid#229024PurposeExpression of a EnAsCas12a guide targeting FOCADDepositorInsertFOCAD gRNA
UseCRISPR and LentiviralTagsExpressionMammalianMutationPromoterAvailable sinceApril 3, 2025AvailabilityAcademic Institutions and Nonprofits only -
pXPR_051d_sgCiChr2-2
Plasmid#228940PurposeExpression of a CRISPRi control sgRNA that cuts an intergenic region on chromosome 2DepositorInsertsgChr2-2
UseCRISPR and LentiviralTagsExpressionMammalianMutationPromoterAvailable sinceApril 3, 2025AvailabilityAcademic Institutions and Nonprofits only -
pTMiniTrex-mCherry-Shark3
Plasmid#233145PurposeServes as a template plasmid for amplifying Shark3 (Theo-OFF) hammerhead aptazyme-3xTy-tagging cassetteDepositorInsertsmCherry
Shark3
UseProkaryotic expressionTags3x Ty tagExpressionMutationCodon optimized for Trypanosoma cruzi and changed…PromoterAvailable sinceMarch 20, 2025AvailabilityAcademic Institutions and Nonprofits only -
pcDNA3.1-myc:FRB:gs10:Cul1(dC501)
Plasmid#228533PurposeA fragment of humanCul1 protein in fusion with FRB domain through a flexible glycine-serine linker.DepositorInsertmyc:FRB:gs10:Cul1(dC501) (CUL1 Synthetic, Human)
UseTagsmycExpressionMammalianMutationPromoterCMVAvailable sinceJan. 3, 2025AvailabilityAcademic Institutions and Nonprofits only -
VPS13B^Halo
Plasmid#224584Purposecodon optimized Halo tagged VPS13B for expression in mammalian cellsDepositorInsertVPS13B^Halo (VPS13B Human)
UseTagsHaloExpressionMammalianMutationPromoterCMVAvailable sinceDec. 20, 2024AvailabilityAcademic Institutions and Nonprofits only -
pCA528 IST1 (1-366)
Plasmid#193039PurposeBacterial expression plasmid for full-length IST1 (1-366).DepositorInsertIST1 (IST1 Human)
UseTags6xHis-SumoExpressionBacterialMutationPromoterT7Available sinceDec. 9, 2024AvailabilityAcademic Institutions and Nonprofits only -
pCA528 CAPN7 (1-75)
Plasmid#193031PurposeBacterial expression plasmid for CAPN7 (1-75) N-terminal MIT domainDepositorInsertCAPN7 (CAPN7 Human)
UseTags6xHis-SumoExpressionBacterialMutationResidues 1-75PromoterT7Available sinceDec. 9, 2024AvailabilityAcademic Institutions and Nonprofits only -
pCA528 CAPN7 (81-165)
Plasmid#193032PurposeBacterial expression plasmid for CAPN7 (81-165) C-terminal MIT domainDepositorInsertCAPN7 (CAPN7 Human)
UseTags6xHis-SumoExpressionBacterialMutationResidues 81-165PromoterT7Available sinceDec. 9, 2024AvailabilityAcademic Institutions and Nonprofits only -
qTAG-C-mStayGold-Puro-H2BC11-MMEJ
Plasmid#227333PurposeMMEJ Donor template for mStayGold-2A-Puro insertion into the C-terminus of the H2BC11 locus. For nuclei visualization. To be co-transfected with sgRNA plasmid px330-PITCh-H2BC11 (Addgene #207755)DepositorInsertH2BC11 Short Homology Arms flanking a mStayGold-2A-Puro Cassette (H2BC11 Human)
UseCRISPR; Donor templateTagsExpressionMammalianMutationPromoterPromoterlessAvailable sinceNov. 12, 2024AvailabilityAcademic Institutions and Nonprofits only -
qTAG-C-Solo-miRFP670nano3-H2BC11
Plasmid#227331PurposeDonor template for miRFP670nano3 insertion into the C-terminus of the H2BC11 locus. For nuclei visualization. To be co-transfected with sgRNA plasmid px330-PITCh-H2BC11 (Addgene #207755)DepositorInsertH2BC11 Homology Arms flanking a miRFP670nano3 Tag (H2BC11 Human)
UseCRISPR; Donor templateTagsExpressionMammalianMutationPromoterPromoterlessAvailable sinceNov. 12, 2024AvailabilityAcademic Institutions and Nonprofits only -
qTAG-N-Solo-mStayGold-TUBA1B
Plasmid#227327PurposeDonor template for mStayGold insertion into the N-terminus of the TUBA1B locus. For tubulin visualization. To be co-transfected with sgRNA plasmid px330-PITCh-TUBA1B (Addgene #207763)DepositorInsertTUBA1B Homology Arms flanking a mStayGold Tag (TUBA1B Human)
UseCRISPR; Donor templateTagsExpressionMammalianMutationPromoterPromoterlessAvailable sinceNov. 12, 2024AvailabilityAcademic Institutions and Nonprofits only -
qTAG-C-Solo-mStayGold-H2BC11
Plasmid#227330PurposeDonor template for mStayGold insertion into the C-terminus of the H2BC11 locus. For nuclei visualization. To be co-transfected with sgRNA plasmid px330-PITCh-H2BC11 (Addgene #207755)DepositorInsertH2BC11 Homology Arms flanking a mStayGold Tag (H2BC11 Human)
UseCRISPR; Donor templateTagsExpressionMammalianMutationPromoterPromoterlessAvailable sinceNov. 12, 2024AvailabilityAcademic Institutions and Nonprofits only -
qTAG-C-mStayGold-EFS-Puro-PLK4
Plasmid#227311PurposeDonor template for mStayGold-EFS-Puro insertion into the C-terminus of the PLK4 locus. For centriole visualization. To be co-transfected with sgRNA plasmid px458-PLK4 (Addgene #227310)DepositorInsertPLK4 Homology Arms flanking a mStayGold-EFS-Puro Cassette (PLK4 Human)
UseCRISPR; Donor templateTagsExpressionMammalianMutationPromoterPromoterlessAvailable sinceNov. 12, 2024AvailabilityAcademic Institutions and Nonprofits only -
qTAG-N-Solo-mStayGold-PCNT
Plasmid#227285PurposeDonor template for mStayGold insertion into the N-terminus of the PCNT locus. For pericentriolar material visualization. To be co-transfected with sgRNA plasmid px330-PCNT (Addgene #227284)DepositorInsertPCNT Homology Arms flanking a mStayGold Tag (PCNT Human)
UseCRISPR; Donor templateTagsExpressionMammalianMutationPromoterPromoterlessAvailable sinceNov. 12, 2024AvailabilityAcademic Institutions and Nonprofits only -
pDEST17-DXO
Plasmid#177993PurposeFor purification of N-terminally His-tagged DXO from bacteriaDepositorInsertDXO (DXO Human)
UseTags6xHisExpressionBacterialMutationPromoterT7Available sinceOct. 23, 2024AvailabilityAcademic Institutions and Nonprofits only -
pMx/ATP9A(E195Q)-HA
Plasmid#209256PurposeMammalian expression of ATP9A mutant E195QDepositorInsertATP9A (ATP9A Human)
UseRetroviralTagsHAExpressionMutationE195QPromoterAvailable sinceSept. 3, 2024AvailabilityAcademic Institutions and Nonprofits only -
pMx/ATP9B(E272Q)-HA
Plasmid#209257PurposeMammalian expression of ATP9B mutant E272QDepositorInsertATP9B (ATP9B Human)
UseRetroviralTagsHAExpressionMutationE272QPromoterAvailable sinceSept. 3, 2024AvailabilityAcademic Institutions and Nonprofits only -
TLNRD1(4H)-2T_pET47b
Plasmid#224069PurposeBacterial expression of the 4-helix bundle of TLNRD1 (residues 143-273) containing the mutations L191T/A225T, fused to an N-terminal hexa-histidine tag. HRV-3C cleavage siteDepositorInserttlnrd1 (TLNRD1 Human)
UseTags6xHISExpressionBacterialMutationL191T/A225T double mutant; reduces affinity of TL…PromoterT7Available sinceAug. 26, 2024AvailabilityIndustry, Academic Institutions, and Nonprofits -
TLNRD1(4H)-2E_pET151
Plasmid#224068PurposeBacterial expression of the 4-helix bundle of TLNRD1 (residues 143-273) containing the mutations K192E/R233E, fused to an N-terminal hexa-histidine tag. TEV cleavage siteDepositorInserttlnrd1 (TLNRD1 Human)
UseTags6xHIS; V5ExpressionBacterialMutationK192E/R233E double mutant; prevents actin binding…PromoterT7Available sinceAug. 26, 2024AvailabilityIndustry, Academic Institutions, and Nonprofits -
pCMV4-HA-p31(comet)
Plasmid#221697PurposeMammalian expression of HA-tagged human p31(comet)DepositorInsertMAD2BP1
UseTagsExpressionMammalianMutationPromoterAvailable sinceAug. 19, 2024AvailabilityAcademic Institutions and Nonprofits only -
pCMV4-HA-MAD2
Plasmid#221696PurposeMammalian expression of HA-tagged human MAD2DepositorInsertMAD2
UseTagsExpressionMammalianMutationPromoterAvailable sinceAug. 19, 2024AvailabilityAcademic Institutions and Nonprofits only -
pAc5.1-EGFP-Vasa 463-661 MUT (F504E) (HK196)
Plasmid#206434PurposeVasa 463-661 expression in Schneider cells for imagingDepositorInsertVasa 463-661 (vas Fly)
UseTagsEGFPExpressionInsectMutationF504EPromoterAvailable sinceJuly 30, 2024AvailabilityAcademic Institutions and Nonprofits only -
pGGI_Myr-YFP-MiniTurboID
Plasmid#222432PurposePotential plasma membrane control.DepositorInsertMiniTurboID (BirA mutant)
UseGolden gate / green gate compatible cloning vectorTagsLinker, Myr, and YFPExpressionMutationaa1-63 deleted; Q65P, I87V, R118S, E140K, Q141R, …PromoterAvailable sinceJuly 29, 2024AvailabilityAcademic Institutions and Nonprofits only -
pAc5.1-PH-mCherry-Oskar 139-240 MUT (A162E/L228E) (HK202)
Plasmid#206464PurposeOskar 139-240 mut expression in Schneider cells for imagingDepositorInsertOskar 139-240 mut (osk Fly)
UseTagsPH mcherryExpressionInsectMutationA162E L228E)PromoterAvailable sinceJuly 24, 2024AvailabilityAcademic Institutions and Nonprofits only