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Enter a sequence to perform a BLAST-based search of Addgene plasmid sequence databases.

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We narrowed to 108,253 results for: nin

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Showing: 341 - 360 of 108253 results

  1. CROPseq-Guide-Puro

    Plasmid
    #86708
    Purpose
    Makes CRISPR gRNAs detectable by single-cell RNA-seq. The gRNA is expressed as part of the puromycin resistance mRNA transcribed by RNA Pol II and in its functional form from the hU6 promoter.
    Depositor
    Christoph Bock
    Publication
    Datlinger et al Nat Methods. 2017 Jan 18. doi: 10.1038/n
    Insert
    EF1a-Puro-WPRE-hU6-gRNA
    Use
    CRISPR and Lentiviral
    Expression
    Mammalian
    Promoter
    hU6
    Available since
    Feb. 9, 2017
    Availability
    Academic Institutions and Nonprofits only
    Cited by
    68 Articles
    86708-plasmid-map-sequence-id-317051

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  2. pC0043-PspCas13b crRNA backbone

    Plasmid
    #103854
    Purpose
    For cloning of guide RNAs compatible with PspCas13b. Contains a 3' direct repeat. Clone using BbsI (BpiI). F overhang cacc. R overhang caac
    Depositor
    Feng Zhang
    Publication
    Cox et al Science. 2017 Oct 25. pii: eaaq0180. doi
    Has service
    Cloning Grade DNA
    Insert
    U6-BbsI-BbsI-PspCas13b DR-polyT
    Use
    CRISPR
    Expression
    Mammalian
    Available since
    Nov. 28, 2017
    Availability
    Industry, Academic Institutions, and Nonprofits
    Cited by
    42 Articles
    103854-plasmid-map-sequence-id-350782

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  3. lentiMPH v2

    Plasmid
    #89308
    Purpose
    lenti vector encoding the MS2-P65-HSF1 activator helper complex with a 2A Hygro resistance marker (EF1a-MS2-p65-HSF1-2A-Hygro-WPRE). This version has a higher virus titer.
    Depositor
    Feng Zhang
    Publication
    Joung et al Nat Protoc. 2017 Apr;12(4):828-863. doi:
    Insert
    MS2-P65-HSF1_2A_Hygro (RELA Human, Synthetic)
    Use
    Lentiviral
    Expression
    Mammalian
    Mutation
    N55K in MS2
    Promoter
    EF1a
    Available since
    March 30, 2017
    Availability
    Academic Institutions and Nonprofits only
    Cited by
    84 Articles
    89308-plasmid-map-sequence-id-425605

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  4. pCW-Cas9-Blast

    Plasmid
    #83481
    Purpose
    Lentiviral vector for mammalian expression of doxycycline-inducible Cas9 and constitutive expression of Blasticidin S deaminase -T2A -rtTA
    Depositor
    Mohan Babu
    Inserts
    SpCas9
    Blasticidin S deaminase -T2A - reverse tetracycline-controlled transactivator
    Use
    CRISPR and Lentiviral
    Expression
    Mammalian
    Mutation
    Replaced puromycin N-acetyltransferase on the ori…
    Promoter
    Tight Tre and human phosphoglycerate kinase 1 pro…
    Available since
    Nov. 10, 2016
    Availability
    Academic Institutions and Nonprofits only
    Cited by
    34 Articles
    83481-plasmid-map-sequence-id-335118

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  5. pcDNA3-CFP

    Plasmid
    #13030
    Purpose
    Mammalian expression vector for cloning your gene fused to CFP
    Depositor
    Doug Golenbock
    Has service
    Cloning Grade DNA
    Type
    Empty backbone
    Expression
    Mammalian
    Available since
    Nov. 3, 2006
    Availability
    Academic Institutions and Nonprofits only
    Cited by
    18 Articles
    13030-plasmid-map-sequence-id-212096

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  7. pBABE GFP

    Plasmid
    #10668
    Purpose
    Empty backbone for retroviral gene expression. Select recipient cells using GFP.
    Depositor
    William Hahn
    Has service
    Cloning Grade DNA
    Type
    Empty backbone
    Use
    Retroviral
    Expression
    Mammalian
    Available since
    Oct. 11, 2005
    Availability
    Academic Institutions and Nonprofits only
    Cited by
    27 Articles
    10668-plasmid-map-sequence-id-106108

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  9. LentiCRISPRv2-mCherry

    Plasmid
    #99154
    Purpose
    Lentiviral vector encoding sgRNA cloning site + hSpCAS9-P2A-mCherry.
    Depositor
    Agata Smogorzewska
    Inserts
    Cas9
    mCherry
    Use
    CRISPR and Lentiviral
    Tags
    FLAG
    Expression
    Mammalian
    Promoter
    EFS-NS
    Available since
    Aug. 10, 2017
    Availability
    Academic Institutions and Nonprofits only
    Cited by
    58 Articles
    99154-plasmid-map-sequence-id-352449

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  10. pcDNA3-mRFP

    Plasmid
    #13032
    Purpose
    Mammalian expression vector for cloning your gene fused to mRFP
    Depositor
    Doug Golenbock
    Has service
    Cloning Grade DNA
    Insert
    Monomer Red Fluorescent Protein
    Expression
    Mammalian
    Available since
    Nov. 7, 2006
    Availability
    Academic Institutions and Nonprofits only
    Cited by
    48 Articles
    13032-plasmid-map-sequence-id-211651

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  13. mCherry2-N1

    Plasmid
    #54517
    Purpose
    Localization: N1 Cloning Vector, Excitation: 587, Emission: 610
    Depositor
    Michael Davidson
    Has service
    Cloning Grade DNA
    Type
    Empty backbone
    Tags
    mCherry2
    Expression
    Mammalian
    Promoter
    CMV
    Available since
    June 12, 2014
    Availability
    Academic Institutions and Nonprofits only
    Cited by
    47 Articles
    54517-plasmid-map-sequence-id-352457

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  14. pRRLSIN.cPPT.PGK-GFP.WPRE

    Plasmid
    #12252
    Purpose
    3rd generation lentiviral backbone
    Depositor
    Didier Trono
    Has service
    Cloning Grade DNA
    Type
    Empty backbone
    Use
    Lentiviral
    Expression
    Mammalian
    Available since
    March 27, 2007
    Availability
    Academic Institutions and Nonprofits only
    Cited by
    190 Articles
    12252-plasmid-map-sequence-id-444115

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  18. pEVOL-pAzF

    Plasmid
    #31186
    Purpose
    tRNA synthetase/tRNA pair for the in vivo incorporation of a photocrosslinker, p-azido-l-phenylalanine, into proteins in E coli response to the amber codon, TAG
    Depositor
    Peter Schultz
    Publication
    Chin et al J Am Chem Soc. 2002 Aug 7;124(31):9026-7
    Insert
    M.j. p-azidohenylalanine RS (2 copies +tRNA)
    Expression
    Bacterial
    Mutation
    Y32T, E107N, D158P, I159L, L162Q, D286R
    Available since
    Dec. 9, 2011
    Availability
    Academic Institutions and Nonprofits only
    Cited by
    52 Articles
    31186-plasmid-map-sequence-id-334781

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Showing: 341 - 360 of 108253 results