We narrowed to 7,306 results for: GFP expression plasmids

1. pCMV-SpCas9-VRQR(+G366R)-P2A-EGFP (KAC1026)

   Plasmid

   #242669

   Purpose

   CMV promoter expression plasmid for human codon optimized SpCas9-VRQR(G366R)-BPNLS(SV40)-3xFLAG-P2A-EGFP

   Depositor

   Benjamin Kleinstiver

   Article

   Alves et al Nat Biomed Eng. 2025 Sep 11:10.1038/s415

   Insert

   pCMV-SpCas9-VRQR(G366R)-BPNLS(SV40)-3xFLAG-P2A-EGFP
   

   Use

   CRISPR

   Tags

   BPNLS

   Expression

   Mammalian

   Mutation

   VRQR + G366R mutations in SpCas9(G366R/D1135V/G12…

   Promoter

   CMV and T7

   Available Since

   Oct. 9, 2025

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

2. pCMV-SpCas9-VRQR(+N1286Q)-P2A-EGFP (LRF247)

   Plasmid

   #242677

   Purpose

   CMV promoter expression plasmid for human codon optimized SpCas9-VRQR(N1286Q)-BPNLS(SV40)-3xFLAG-P2A-EGFP

   Depositor

   Benjamin Kleinstiver

   Article

   Alves et al Nat Biomed Eng. 2025 Sep 11:10.1038/s415

   Insert

   pCMV-SpCas9-VRQR(N1286Q)-BPNLS(SV40)-3xFLAG-P2A-EGFP
   

   Use

   CRISPR

   Tags

   BPNLS

   Expression

   Mammalian

   Mutation

   VRQR + N1286Q mutations in SpCas9(D1135V/G1218R/N…

   Promoter

   CMV and T7

   Available Since

   Oct. 9, 2025

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

3. pCMV-SpCas9-VRQR(+G1104K)-P2A-EGFP (LRF54)

   Plasmid

   #242671

   Purpose

   CMV promoter expression plasmid for human codon optimized SpCas9-VRQR(G1140K)-BPNLS(SV40)-3xFLAG-P2A-EGFP

   Depositor

   Benjamin Kleinstiver

   Article

   Alves et al Nat Biomed Eng. 2025 Sep 11:10.1038/s415

   Insert

   pCMV-SpCas9-VRQR(G1140K)-BPNLS(SV40)-3xFLAG-P2A-EGFP
   

   Use

   CRISPR

   Tags

   BPNLS

   Expression

   Mammalian

   Mutation

   VRQR + G1104K mutations in SpCas9(G1104K/D1135V/G…

   Promoter

   CMV and T7

   Available Since

   Oct. 9, 2025

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

4. pCMV-SpCas9-VRQR(+A61R)-P2A-EGFP (KAC1030)

   Plasmid

   #242665

   Purpose

   CMV promoter expression plasmid for human codon optimized SpCas9-VRQR(A61R)-BPNLS(SV40)-3xFLAG-P2A-EGFP

   Depositor

   Benjamin Kleinstiver

   Article

   Alves et al Nat Biomed Eng. 2025 Sep 11:10.1038/s415

   Insert

   pCMV-SpCas9-VRQR(A61R)-BPNLS(SV40)-3xFLAG-P2A-EGFP
   

   Use

   CRISPR

   Tags

   BPNLS

   Expression

   Mammalian

   Mutation

   VRQR + A61R mutations in SpCas9(A61R/D1135V/G1218…

   Promoter

   CMV and T7

   Available Since

   Oct. 9, 2025

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

5. pCMV-SpCas9-VRQR(+L244Q)-P2A-EGFP (LRF96)

   Plasmid

   #242667

   Purpose

   CMV promoter expression plasmid for human codon optimized SpCas9-VRQR(L244Q)-BPNLS(SV40)-3xFLAG-P2A-EGFP

   Depositor

   Benjamin Kleinstiver

   Article

   Alves et al Nat Biomed Eng. 2025 Sep 11:10.1038/s415

   Insert

   pCMV-SpCas9-VRQR(L244Q)-BPNLS(SV40)-3xFLAG-P2A-EGFP
   

   Use

   CRISPR

   Tags

   BPNLS

   Expression

   Mammalian

   Mutation

   VRQR + L244Q mutations in SpCas9(L244Q/D1135V/G12…

   Promoter

   CMV and T7

   Available Since

   Oct. 9, 2025

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

6. pCMV-SpCas9-VRQR(+N1317R)-P2A-EGFP (LRF53)

   Plasmid

   #242678

   Purpose

   CMV promoter expression plasmid for human codon optimized SpCas9-VRQR(N1317R)-BPNLS(SV40)-3xFLAG-P2A-EGFP

   Depositor

   Benjamin Kleinstiver

   Article

   Alves et al Nat Biomed Eng. 2025 Sep 11:10.1038/s415

   Insert

   pCMV-SpCas9-VRQR(N1317R)-BPNLS(SV40)-3xFLAG-P2A-EGFP
   

   Use

   CRISPR

   Tags

   BPNLS

   Expression

   Mammalian

   Mutation

   VRQR + N1317R mutations in SpCas9(D1135V/G1218R/N…

   Promoter

   CMV and T7

   Available Since

   Oct. 9, 2025

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

7. pCMV-SpCas9-VRQR(+A1285K)-P2A-EGFP (KAC1134)

   Plasmid

   #242676

   Purpose

   CMV promoter expression plasmid for human codon optimized SpCas9-VRQR(A1285K)-BPNLS(SV40)-3xFLAG-P2A-EGFP

   Depositor

   Benjamin Kleinstiver

   Article

   Alves et al Nat Biomed Eng. 2025 Sep 11:10.1038/s415

   Insert

   pCMV-SpCas9-VRQR(A1285K)-BPNLS(SV40)-3xFLAG-P2A-EGFP
   

   Use

   CRISPR

   Tags

   BPNLS

   Expression

   Mammalian

   Mutation

   VRQR + A1285K mutations in SpCas9(D1135V/G1218R/A…

   Promoter

   CMV and T7

   Available Since

   Oct. 9, 2025

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

8. pCMV-SpCas9-VRQR(+L1245V)-P2A-EGFP (LRF49)

   Plasmid

   #242675

   Purpose

   CMV promoter expression plasmid for human codon optimized SpCas9-VRQR(L1245V)-BPNLS(SV40)-3xFLAG-P2A-EGFP

   Depositor

   Benjamin Kleinstiver

   Article

   Alves et al Nat Biomed Eng. 2025 Sep 11:10.1038/s415

   Insert

   pCMV-SpCas9-VRQR(L1245V)-BPNLS(SV40)-3xFLAG-P2A-EGFP
   

   Use

   CRISPR

   Tags

   BPNLS

   Expression

   Mammalian

   Mutation

   VRQR + L1245V mutations in SpCas9(D1135V/G1218R/L…

   Promoter

   CMV and T7

   Available Since

   Oct. 9, 2025

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

9. pCMV-SpCas9-VRQR(+D1332R)-P2A-EGFP (LRF45)

   Plasmid

   #242681

   Purpose

   CMV promoter expression plasmid for human codon optimized SpCas9-VRQR(D1332R)-BPNLS(SV40)-3xFLAG-P2A-EGFP

   Depositor

   Benjamin Kleinstiver

   Article

   Alves et al Nat Biomed Eng. 2025 Sep 11:10.1038/s415

   Insert

   pCMV-SpCas9-VRQR(D1332R)-BPNLS(SV40)-3xFLAG-P2A-EGFP
   

   Use

   CRISPR

   Tags

   BPNLS

   Expression

   Mammalian

   Mutation

   VRQR + D1332R mutations in SpCas9(D1135V/G1218R/D…

   Promoter

   CMV and T7

   Available Since

   Oct. 9, 2025

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

10. pCMV-SpCas9-VRQR(+L1111H)-P2A-EGFP (LRF269)

    Plasmid

    #242672

    Purpose

    CMV promoter expression plasmid for human codon optimized SpCas9-VRQR(L1111H)-BPNLS(SV40)-3xFLAG-P2A-EGFP

    Depositor

    Benjamin Kleinstiver

    Article

    Alves et al Nat Biomed Eng. 2025 Sep 11:10.1038/s415

    Insert

    pCMV-SpCas9-VRQR(L1111H)-BPNLS(SV40)-3xFLAG-P2A-EGFP
    

    Use

    CRISPR

    Tags

    BPNLS

    Expression

    Mammalian

    Mutation

    VRQR + L1111H mutations in SpCas9(L1111H/D1135V/G…

    Promoter

    CMV and T7

    Available Since

    Oct. 9, 2025

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

11. pCMV-SpCas9-VRQR(+T1138K)-P2A-EGFP (LRF243)

    Plasmid

    #242674

    Purpose

    CMV promoter expression plasmid for human codon optimized SpCas9-VRQR(T1138K)-BPNLS(SV40)-3xFLAG-P2A-EGFP

    Depositor

    Benjamin Kleinstiver

    Article

    Alves et al Nat Biomed Eng. 2025 Sep 11:10.1038/s415

    Insert

    pCMV-SpCas9-VRQR(T1138K)-BPNLS(SV40)-3xFLAG-P2A-EGFP
    

    Use

    CRISPR

    Tags

    BPNLS

    Expression

    Mammalian

    Mutation

    VRQR + T1138K mutations in SpCas9(D1135V/T1138K/G…

    Promoter

    CMV and T7

    Available Since

    Oct. 9, 2025

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

12. pCMV-SpCas9-VRQR(+D1332K)-P2A-EGFP (KAC966)

    Plasmid

    #242680

    Purpose

    CMV promoter expression plasmid for human codon optimized SpCas9-VRQR(D1332K)-BPNLS(SV40)-3xFLAG-P2A-EGFP

    Depositor

    Benjamin Kleinstiver

    Article

    Alves et al Nat Biomed Eng. 2025 Sep 11:10.1038/s415

    Insert

    pCMV-SpCas9-VRQR(D1332K)-BPNLS(SV40)-3xFLAG-P2A-EGFP
    

    Use

    CRISPR

    Tags

    BPNLS

    Expression

    Mammalian

    Mutation

    VRQR + D1332K mutations in SpCas9(D1135V/G1218R/D…

    Promoter

    CMV and T7

    Available Since

    Oct. 9, 2025

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

13. pAAV-SynI-CreOn-FlpOff-mNaChBac-P2A-EGFP

    Plasmid

    #176280

    Purpose

    Viral vector for co-expression of NaChBac and EGFP in cells expressing Cre AND NOT Flp driven by the human Synapsin promoter.

    Depositor

    Martin Riccomagno

    Article

    Ubina et al J Cell Sci. 2021 Oct 15;134(20). pii: 27

    Insert

    mNaChBac-P2A-EGFP
    

    Use

    AAV and Cre/Lox

    Expression

    Mammalian

    Promoter

    human Synapsin I

    Available Since

    Jan. 11, 2022

    Availability

    Academic Institutions and Nonprofits only

    

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14. pAAV-SynI-CreOn-FlpOff-mNaChBacMUT-P2A-EGFP

    Plasmid

    #176281

    Purpose

    Viral vector for co-expression of non-functional NaChBac and EGFP in cells expressing Cre AND NOT Flp driven by the human Synapsin promoter.

    Depositor

    Martin Riccomagno

    Article

    Ubina et al J Cell Sci. 2021 Oct 15;134(20). pii: 27

    Insert

    mNaChBacMUT-P2A-EGFP
    

    Use

    AAV and Cre/Lox

    Expression

    Mammalian

    Mutation

    E191K

    Promoter

    human Synapsin I

    Available Since

    Jan. 11, 2022

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

15. pAAV-SynI-CreOn-FlpOff-MCS-P2A-EGFP

    Plasmid

    #176276

    Purpose

    Viral vector for co-expression of a CDS of interest and EGFP in cells expressing Cre AND NOT Flp driven by a Synapsin promoter. Contains an in-frame P2A sequence and an MCS for cloning of the CDS.

    Depositor

    Martin Riccomagno

    Article

    Ubina et al J Cell Sci. 2021 Oct 15;134(20). pii: 27

    Insert

    MCS-P2A-EGFP
    

    Use

    AAV and Cre/Lox

    Expression

    Mammalian

    Promoter

    human Synapsin I

    Available Since

    Dec. 17, 2021

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

16. G718A_BattB(GA)_TKterm_AttB(GT)_sGFP-PTEN-IRES-mCherry-P2A-HygroR_noTerm_attP(GA)_IRES-UnaG-2A-Puro-Term

    Plasmid

    #200634

    Purpose

    Plasmid self-excising recombination plasmid for Matreyek Bxb1(GT) landing pad

    Depositor

    Kenneth Matreyek

    Article

    Roelle et al ACS Synth Biol. 2023 Nov 3. doi: 10.1021

    Insert

    PTEN (PTEN Human)
    

    Expression

    Mammalian

    Available Since

    Nov. 6, 2023

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

17. pCMV-T7-SpRY-P2A-EGFP (RTW4830)

    Plasmid

    #139989

    Purpose

    CMV and T7 promoter expression plasmid for human codon optimized SpRY(A61R/L1111R/D1135L/S1136W/G1218K/E1219Q/N1317R/A1322R/R1333P/R1335Q/T1337R) with a c-terminal bi-partite NLS, 3x flag tag, and P2A-EGFP

    Depositor

    Benjamin Kleinstiver

    Article

    Walton et al Science. 2020 Apr 17;368(6488):290-296.

    Insert

    human codon optimized SpCas9 variant named SpRY with BPNLS-3xFLAG-P2A-EGFP
    

    Use

    CRISPR; In vitro transcription; t7 promoter

    Tags

    BPNLS-3xFLAG-P2A-EGFP

    Expression

    Mammalian

    Mutation

    SpRY=A61R/L1111R/D1135L/S1136W/G1218K/E1219Q/N131…

    Promoter

    CMV and T7

    Available Since

    March 26, 2020

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

18. GC52 pEGFPN1 anti-Gephyrin nanobody

    Plasmid

    #135224

    Purpose

    Mammalian expression of anti-Gephyrin (Human) recombinant llama nanobody for use as an intrabody

    Depositor

    James Trimmer

    Article

    Dong et al Elife. 2019 Sep 30;8. pii: 48750. doi: 1

    Insert

    anti-Gephyrin (Human) recombinant llama nanobody GC52 (GPHN L. glama (llama))
    

    Tags

    6xHis-HA-EGFP

    Expression

    Mammalian

    Available Since

    Dec. 18, 2019

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

19. pDRM210 LGP (Luciferase GFP Puro)

    Plasmid

    #174722

    Purpose

    Expresses the firefly luciferase gene, E2A, eGFP, F2A, and the puromycin resistance gene

    Depositor

    Wayne Tilley

    Article

    Mustafa et al Oncogene. 2023 Nov 24. doi: 10.1038/s413

    Inserts

    Firefly Luciferase
    eGFP
    pac
    

    Use

    Lentiviral and Luciferase

    Tags

    E2A linker and F2A linker

    Expression

    Mammalian

    Promoter

    MND, MND (off Luc-E2A), and MND (off Luc-E2A-eGFP…

    Available Since

    Nov. 30, 2023

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

20. TRE-UBC-hCD59-T2A-GFP

    Plasmid

    #205460

    Purpose

    lentiviral plasmid for expression of human CD59

    Depositor

    Deepta Bhattacharya

    Article

    Pizzato et al Stem Cell Reports. 2023 Dec 22:S2213-671

    Insert

    hCD59 (CD59 Human)
    

    Expression

    Mammalian

    Promoter

    UBC

    Available Since

    Sept. 27, 2023

    Availability

    Academic Institutions and Nonprofits only

    

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