We narrowed to 14,086 results for: cas9

1. pFREE_Cm

   Plasmid

   #92052

   Purpose

   Allows inducible expression of gRNAs and Cas9 for plasmid curing.

   Depositor

   Morten Norholm

   Article

   Lauritsen et al Microb Cell Fact. 2017 Aug 2;16(1):135.

   Inserts

   Cas9
   gRNA array
   

   Use

   CRISPR

   Promoter

   pRhamBAD and ptet

   Available Since

   Aug. 3, 2017

   Availability

   Academic Institutions and Nonprofits only

   

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2. vHB8

   Plasmid

   #193454

   Purpose

   Expression of GFP-Cas9-NLS and gRNA in A. castellanii

   Depositor

   Chantal Abergel

   Article

   Bisio et al Nat Commun. 2023 Jan 26;14(1):428. doi:

   Insert

   GFP-Cas9-NLS
   

   Use

   CRISPR

   Tags

   GFP

   Promoter

   GAPDH

   Available Since

   Jan. 17, 2023

   Availability

   Academic Institutions and Nonprofits only

   

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3. pFREE_Zeo

   Plasmid

   #92053

   Purpose

   Allows inducible expression of gRNAs and Cas9 for plasmid curing.

   Depositor

   Morten Norholm

   Article

   Lauritsen et al Microb Cell Fact. 2017 Aug 2;16(1):135.

   Inserts

   Cas9
   gRNA array
   

   Use

   CRISPR

   Promoter

   pRhamBAD and ptet

   Available Since

   June 12, 2017

   Availability

   Academic Institutions and Nonprofits only

   

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4. pX330A-1x6

   Plasmid

   #58770

   Purpose

   Expresses Cas9 nuclease and gRNA

   Depositor

   Takashi Yamamoto

   Article

   Sakuma et al Sci Rep. 2014 Jun 23;4:5400. doi: 10.103

   Insert

   humanized S. pyogenes Cas9 nuclease
   

   Use

   CRISPR

   Tags

   3xFLAG

   Expression

   Mammalian

   Promoter

   CBh

   Available Since

   Nov. 7, 2014

   Availability

   Academic Institutions and Nonprofits only

   

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5. pMLS485

   Plasmid

   #188890

   Purpose

   Worm Cas9 expression vector, Peft-3 promoter

   Depositor

   Erik Jorgensen

   Article

   Schwartz et al PLoS Genet. 2021 Nov 8;17(11):e1009755.

   Insert

   Peft-3::Cas9
   

   Expression

   Worm

   Mutation

   C. elegans codom optimization and intron addition

   Available Since

   Sept. 6, 2022

   Availability

   Academic Institutions and Nonprofits only

   

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6. pS448∙CsR

   Plasmid

   #122096

   Purpose

   CRISPR/Cas9 counterselection in Gram-negative bacteria

   Depositor

   Pablo Ivan Nikel

   Article

   Wirth et al Microb Biotechnol. 2019 Mar 12. doi: 10.

   Inserts

   Cas9
   sgRNA
   

   Use

   Synthetic Biology

   Expression

   Bacterial

   Promoter

   Pem7 and Pm

   Available Since

   April 10, 2019

   Availability

   Academic Institutions and Nonprofits only

   

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7. pBBK28 pHomL-TDH3p-GFPdropout-Link-Neon(gfp)-SSA1t-HomR (AmpR)

   Plasmid

   #179012

   Purpose

   Parent vector for markerless yeast integration cassettes with C-terminal Neon(gfp)-tagged genes

   Depositor

   Vincent Martin

   Article

   Bean et al G3 (Bethesda). 2022 Jun 16. pii: 6609175

   Type

   Empty backbone

   Expression

   Yeast

   Available Since

   Aug. 5, 2022

   Availability

   Academic Institutions and Nonprofits only

   

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8. pJB06

   Plasmid

   #190480

   Purpose

   Base Cas9 expression plasmid for 2-plasmid C. difficile mutagenesis system

   Depositor

   Joseph Sorg

   Article

   Brehm et al Microbiol Resour Announc. 2022 Dec 15;11

   Inserts

   Cas9
   XylR
   

   Use

   E. coli / b. subtilis - c. difficile shuttle vect…

   Promoter

   xylR-driven promoter

   Available Since

   Oct. 18, 2022

   Availability

   Academic Institutions and Nonprofits only

   

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9. pDHt/sk-PC

   Plasmid

   #92125

   Purpose

   Expression of codon-optimized Cas9 with Ppdc promoter in Trichoderma reesei

   Depositor

   Zhihua Zhou

   Article

   Liu et al Cell Discov. 2015 May 12;1:15007. doi: 1

   Insert

   Cas9
   

   Use

   CRISPR; Trichoderma reesei expression

   Promoter

   Ppdc

   Available Since

   July 17, 2018

   Availability

   Academic Institutions and Nonprofits only

   

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10. DS-SPcas

    Plasmid

    #48645

    Purpose

    Bacterial S. pyogenes Cas9 (SP) + tracrRNA expression, cloDF13/spectinomycin

    Depositor

    George Church

    Article

    Esvelt et al Nat Methods. 2013 Sep 29. doi: 10.1038/n

    Inserts

    Cas9
    tracrRNA precursor
    

    Use

    CRISPR

    Promoter

    proC and tracrRNA promoter

    Available Since

    Dec. 2, 2013

    Availability

    Academic Institutions and Nonprofits only

    

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11. pX330S-7

    Plasmid

    #58783

    Purpose

    Expresses Cas9 nuclease and gRNA

    Depositor

    Takashi Yamamoto

    Article

    Sakuma et al Sci Rep. 2014 Jun 23;4:5400. doi: 10.103

    Insert

    humanized S. pyogenes Cas9 nuclease
    

    Use

    CRISPR

    Tags

    3xFLAG

    Expression

    Mammalian

    Promoter

    CBh

    Available Since

    Nov. 7, 2014

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

12. pX330A-1x3

    Plasmid

    #58767

    Purpose

    Expresses Cas9 nuclease and gRNA

    Depositor

    Takashi Yamamoto

    Article

    Sakuma et al Sci Rep. 2014 Jun 23;4:5400. doi: 10.103

    Insert

    humanized S. pyogenes Cas9 nuclease
    

    Use

    CRISPR

    Tags

    3xFLAG

    Expression

    Mammalian

    Promoter

    CBh

    Available Since

    Nov. 7, 2014

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

13. pDHt/sk-CC

    Plasmid

    #92127

    Purpose

    Expression of codon-optimized Cas9 with Pcbh1 promoter in Trichoderma reesei

    Depositor

    Zhihua Zhou

    Article

    Liu et al Cell Discov. 2015 May 12;1:15007. doi: 1

    Insert

    Cas9
    

    Use

    CRISPR; Trichoderma reesei expression

    Promoter

    Pcbh1

    Available Since

    Nov. 2, 2017

    Availability

    Academic Institutions and Nonprofits only

    

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14. pX330S-5

    Plasmid

    #58781

    Purpose

    Expresses Cas9 nuclease and gRNA

    Depositor

    Takashi Yamamoto

    Article

    Sakuma et al Sci Rep. 2014 Jun 23;4:5400. doi: 10.103

    Insert

    humanized S. pyogenes Cas9 nuclease
    

    Use

    CRISPR

    Tags

    3xFLAG

    Expression

    Mammalian

    Promoter

    CBh

    Available Since

    Nov. 7, 2014

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

15. pX330S-6

    Plasmid

    #58782

    Purpose

    Expresses Cas9 nuclease and gRNA

    Depositor

    Takashi Yamamoto

    Article

    Sakuma et al Sci Rep. 2014 Jun 23;4:5400. doi: 10.103

    Insert

    humanized S. pyogenes Cas9 nuclease
    

    Use

    CRISPR

    Tags

    3xFLAG

    Expression

    Mammalian

    Promoter

    CBh

    Available Since

    Nov. 7, 2014

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

16. M-ST1cas

    Plasmid

    #48669

    Purpose

    Mammalian S. thermophilus #1 Cas9 expression, human optimized

    Depositor

    George Church

    Article

    Esvelt et al Nat Methods. 2013 Sep 29. doi: 10.1038/n

    Insert

    Cas9
    

    Use

    CRISPR

    Tags

    NLS

    Promoter

    CMV

    Available Since

    Oct. 16, 2013

    Availability

    Academic Institutions and Nonprofits only

    

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17. pX330A-1x4

    Plasmid

    #58768

    Purpose

    Expresses Cas9 nuclease and gRNA

    Depositor

    Takashi Yamamoto

    Article

    Sakuma et al Sci Rep. 2014 Jun 23;4:5400. doi: 10.103

    Insert

    humanized S. pyogenes Cas9 nuclease
    

    Use

    CRISPR

    Tags

    3xFLAG

    Expression

    Mammalian

    Promoter

    CBh

    Available Since

    Nov. 7, 2014

    Availability

    Academic Institutions and Nonprofits only

    

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18. M-NMcas

    Plasmid

    #48670

    Purpose

    Mammalian N. meningitidis Cas9 expression, human optimized

    Depositor

    George Church

    Article

    Esvelt et al Nat Methods. 2013 Sep 29. doi: 10.1038/n

    Insert

    Cas9
    

    Use

    CRISPR

    Tags

    NLS

    Promoter

    CMV

    Available Since

    Jan. 9, 2014

    Availability

    Academic Institutions and Nonprofits only

19. pDB4280

    Plasmid

    #98699

    Purpose

    A Cas9-encoding plasmid containing the 5' portion of the ura4 marker and the rrk1 promoter/leader. When linearized by NotI, it serves as the gapped vector in the split-ura4 system

    Depositor

    Li-Lin Du

    Article

    Zhang et al G3 (Bethesda). 2018 Apr 27. pii: g3.118.

    Insert

    Cas9
    

    Use

    CRISPR

    Expression

    Yeast

    Available Since

    Jan. 2, 2018

    Availability

    Academic Institutions and Nonprofits only

    

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20. pX330A-1x5

    Plasmid

    #58769

    Purpose

    Expresses Cas9 nuclease and gRNA

    Depositor

    Takashi Yamamoto

    Article

    Sakuma et al Sci Rep. 2014 Jun 23;4:5400. doi: 10.103

    Insert

    humanized S. pyogenes Cas9 nuclease
    

    Use

    CRISPR

    Tags

    3xFLAG

    Expression

    Mammalian

    Promoter

    CBh

    Available Since

    Nov. 7, 2014

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge