We narrowed to 6,697 results for: Lta;

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pAAVS1-NC-CRISPRi (Gen3)

Plasmid

#73499

Purpose

Constitutive CRISPR interference (CRISPRi) knock in construct into the AAVS1 locus

Depositor

Bruce Conklin

Article

Mandegar et al Cell Stem Cell. 2016 Apr 7;18(4):541-53.

Insert

dCas9-KRAB

Use

CRISPR

Tags

HA, KRAB, and NLS

Expression

Mammalian

Mutation

D10A, H840A (catalytically deactivated Cas9)

Promoter

CAG

Available Since

Dec. 7, 2016

Availability

Academic Institutions and Nonprofits only

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AAV-hSynI-rtTAV16

Plasmid

#102367

Purpose

AAV vector to drive TET-ON activator expression from human synapsin I promoter

Depositor

Tetsuo Yamamori

Article

Watakabe et al Front Neural Circuits. 2014 Sep 17;8:110

Insert

rtTAV16

Use

AAV

Expression

Mammalian

Mutation

rtTA variant reported in Gene Therapy (2006) 13, …

Promoter

human synapsin I promoter

Available Since

Jan. 9, 2018

Availability

Academic Institutions and Nonprofits only

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pLL3.7m-mTurquoise2-SLBP(18-126)-IRES-H1-mMaroon1

Plasmid

#83842

Purpose

Fluorescent probe for S/G2 cell cycle transition and G2/M transition

Depositor

Michael Lin

Article

Bajar et al Nat Methods. 2016 Oct 31. doi: 10.1038/n

Insert

mTurquoise2-SLBP(18-126)-IRES-H1-mMaroon1 (H1-0, SLBP Human, Synthetic)

Use

Lentiviral

Expression

Mammalian

Promoter

CMV

Available Since

Nov. 3, 2016

Availability

Academic Institutions and Nonprofits only

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pgRNA-CKB

Plasmid

#73501

Purpose

gRNA expression vector (with mKate2)

Depositor

Bruce Conklin

Article

Mandegar et al Cell Stem Cell. 2016 Apr 7;18(4):541-53.

Insert

mKate2-T2A-Bsd

Use

CRISPR and Lentiviral

Tags

NLS

Expression

Mammalian

Promoter

CAG

Available Since

Dec. 7, 2016

Availability

Academic Institutions and Nonprofits only

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pMEL13

Plasmid

#107919

Purpose

kan based Yeast/E.coli shuttle vector designed to clone and express Cas9 programming spacer

Depositor

Jean-Marc Daran

Article

Mans et al FEMS Yeast Res. 2015 Mar;15(2). pii: fov

Insert

gRNA-CAN1.Y

Use

CRISPR

Expression

Yeast

Available Since

April 18, 2018

Availability

Academic Institutions and Nonprofits only

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pROS10

Plasmid

#107924

Purpose

URA3 based Yeast/E.coli shuttle vector designed to clone and express Cas9 programming spacer

Depositor

Jean-Marc Daran

Article

Mans et al FEMS Yeast Res. 2015 Mar;15(2). pii: fov

Insert

gRNA-CAN1.Y, gRNA-ADE2.Y

Use

CRISPR

Expression

Yeast

Available Since

April 18, 2018

Availability

Academic Institutions and Nonprofits only

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AAV-aCamkII-mCherry-P2A-Cre-WPRE-BGH-polyA

Plasmid

#107313

Purpose

Encoding aCamkII-mCherry-Cre

Depositor

Hui Yang

Article

Zhou et al Nat Neurosci. 2018 Jan 15. pii: 10.1038/

Insert

aCamkII-mCherry-P2A-Cre-WPRE-BGH-polyA

Use

AAV

Expression

Mammalian

Available Since

March 7, 2019

Availability

Academic Institutions and Nonprofits only

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pTRIP-SFFV-mtagBFP-2A STING-342STOP

Plasmid

#102588

Purpose

Lentivector to express STING delta 342 and mTagBFP

Depositor

Nicolas Manel

Article

Cerboni et al J Exp Med. 2017 Jun 5;214(6):1769-1785.

Insert

STING (STING1 Human)

Use

Lentiviral

Expression

Mammalian

Mutation

delta 342

Promoter

SFFV

Available Since

Jan. 25, 2018

Availability

Academic Institutions and Nonprofits only

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EF1α-scFv-p65-HSF1-T2A-EGFP-WPRE-PolyA

Plasmid

#107311

Purpose

Encoding scFv-p65-HSF1 driven by EF1α promoter

Depositor

Hui Yang

Article

Zhou et al Nat Neurosci. 2018 Jan 15. pii: 10.1038/

Insert

EF1α-scFv-p65-HSF1-T2A-EGFP-WPRE-PolyA

Use

Lentiviral

Expression

Mammalian

Available Since

March 7, 2019

Availability

Academic Institutions and Nonprofits only

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pCMV-hARΔ142–337

Plasmid

#89106

Purpose

Mammalian expression of human androgen receptor with deletion of amino acids 142-337 N-terminal transactivation function 1

Depositor

Elizabeth Wilson

Article

Titus et al PLoS One. 2012;7(1):e30192. doi: 10.1371

Insert

hAR delta 142-337 (AR Human)

Expression

Mammalian

Mutation

hAR delta 142-337

Promoter

CMV

Available Since

April 12, 2017

Availability

Academic Institutions and Nonprofits only
pAAV-hsyn-flex-Voltron-ST

Plasmid

#119036

Purpose

Cre-dependant expression of soma-localized Voltron in neurons

Depositor

Eric Schreiter

Article

Abdelfattah et al Science. 2019 Aug 1. pii: science.aav641

Has Service

AAV1

Insert

Voltron-ST

Use

AAV and Cre/Lox

Expression

Mammalian

Promoter

hsyn

Available Since

Dec. 19, 2018

Availability

Academic Institutions and Nonprofits only

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pTRIP-SFFV-mtagBFP-2A STING 368STOP

Plasmid

#102590

Purpose

Lentivector to express STING delta 368 and mTagBFP

Depositor

Nicolas Manel

Article

Cerboni et al J Exp Med. 2017 Jun 5;214(6):1769-1785.

Insert

STING (STING1 Human)

Use

Lentiviral

Expression

Mammalian

Mutation

delta 368

Promoter

SFFV

Available Since

Jan. 17, 2018

Availability

Academic Institutions and Nonprofits only

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pCI-neo-RL-ZEB1 200bmutx5

Plasmid

#35537

Depositor

Greg Goodall

Article

Gregory et al Nat Cell Biol. 2008 May;10(5):593-601. E

Insert

3'UTR of ZEB1 (ZEB1 Human, Budding Yeast)

Use

Luciferase

Expression

Mammalian

Mutation

5 mutations in miR200b seed regions of 3'UTR…

Available Since

May 10, 2012

Availability

Academic Institutions and Nonprofits only

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pBKWH-p53

Plasmid

#133900

Purpose

Positive control when used in combination with pAWH-largeT. Expression of Gal4BD-p53 hybrid protein. Homology regions for recombination with pAWH

Depositor

Sebastian Maurer

Article

Yang et al Nat Commun. 2018 Sep 14;9(1):3747. doi:

Insert

p53 (Trp53 Mouse)

Expression

Yeast

Promoter

T7

Available Since

June 11, 2020

Availability

Academic Institutions and Nonprofits only

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pROS17

Plasmid

#107931

Purpose

TRP1 based Yeast/E.coli shuttle vector designed to clone and express Cas9 programming spacer

Depositor

Jean-Marc Daran

Article

Mans et al FEMS Yeast Res. 2015 Mar;15(2). pii: fov

Insert

gRNA-CAN1.Y, gRNA-ADE2.Y

Use

CRISPR

Expression

Yeast

Available Since

April 18, 2018

Availability

Academic Institutions and Nonprofits only

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pDisplay-DNER

Plasmid

#51053

Purpose

expression of HA-DNER in mammalian cells

Depositor

Mineko Kengaku

Article

Eiraku et al J Biol Chem. 2002 Jul 12;277(28):25400-7

Insert

DNER (Dner Mouse)

Tags

HA and signal peptide

Expression

Mammalian

Mutation

without endogenous signal peptide (1-221bp)

Promoter

CMV

Available Since

Feb. 19, 2014

Availability

Academic Institutions and Nonprofits only
pTRIP-SFFV-mtagBFP-2A STING-354STOP

Plasmid

#102589

Purpose

Lentivector to express STING delta 354 and mTagBFP

Depositor

Nicolas Manel

Article

Cerboni et al J Exp Med. 2017 Jun 5;214(6):1769-1785.

Insert

STING (STING1 Human)

Use

Lentiviral

Expression

Mammalian

Mutation

delta 354

Promoter

SFFV

Available Since

Jan. 17, 2018

Availability

Academic Institutions and Nonprofits only

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pROS11

Plasmid

#107925

Purpose

amdS based Yeast/E.coli shuttle vector designed to clone and express Cas9 programming spacer

Depositor

Jean-Marc Daran

Article

Mans et al FEMS Yeast Res. 2015 Mar;15(2). pii: fov

Insert

gRNA-CAN1.Y, gRNA-ADE2.Y

Use

CRISPR

Expression

Yeast

Available Since

April 18, 2018

Availability

Academic Institutions and Nonprofits only

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pROS16

Plasmid

#107930

Purpose

HIS3 based Yeast/E.coli shuttle vector designed to clone and express Cas9 programming spacer

Depositor

Jean-Marc Daran

Article

Mans et al FEMS Yeast Res. 2015 Mar;15(2). pii: fov

Insert

gRNA-CAN1.Y, gRNA-ADE2.Y

Use

CRISPR

Expression

Yeast

Available Since

April 18, 2018

Availability

Academic Institutions and Nonprofits only

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KC1D_HUMAN_D0

Plasmid

#79723

Purpose

This plasmid encodes the kinase domain of KC1D. Intended for co-expression with Lambda Phosphatase that accompanies this set to enhance bacterial kinase expression.

Depositor

John Chodera, Nicholas Levinson, Markus Seeliger

Article

Albanese et al Biochemistry. 2018 Jul 13. doi: 10.1021/

Insert

KC1D (CSNK1D Human)

Tags

His10-TEV

Expression

Bacterial

Promoter

T7

Available Since

Nov. 21, 2016

Availability

Academic Institutions and Nonprofits only