We narrowed to 1,622 results for: nbas

1. sgRNA BRCA1 S1363L

   Plasmid

   #139329

   Purpose

   Plasmid expressing a sgRNA to introduce BRCA1 S1363L using base editing

   Depositor

   Alberto Ciccia

   Article

   Billon et al Cell Rep. 2020 Mar 10;30(10):3280-3295.e

   Insert

   sgRNA to insert BRCA1 S1363L using base editing
   

   Use

   Tags

   Expression

   Mammalian

   Mutation

   Promoter

   U6

   Available since

   May 22, 2020

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

2. sgRNA BRCA1 E1754K

   Plasmid

   #139330

   Purpose

   Plasmid expressing a sgRNA to introduce BRCA1 E1754K using base editing

   Depositor

   Alberto Ciccia

   Article

   Billon et al Cell Rep. 2020 Mar 10;30(10):3280-3295.e

   Insert

   sgRNA to insert BRCA1 E1754K using base editing
   

   Use

   Tags

   Expression

   Mammalian

   Mutation

   Promoter

   U6

   Available since

   May 22, 2020

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

3. sgRNA FANCD2 Q223Stop

   Plasmid

   #139332

   Purpose

   Plasmid expressing a sgRNA to introduce FANCD2 Q223Stop using base editing

   Depositor

   Alberto Ciccia

   Article

   Billon et al Cell Rep. 2020 Mar 10;30(10):3280-3295.e

   Insert

   sgRNA to insert FANCD2 Q223Stop using base editing
   

   Use

   Tags

   Expression

   Mammalian

   Mutation

   Promoter

   U6

   Available since

   May 22, 2020

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

4. mOC-STAMP (N162D)_3xFlag-Myc-CMV26

   Plasmid

   #73578

   Purpose

   Express mouse OC-STAMP ((N162D) in mammalian cells

   Depositor

   Paul Odgren

   Article

   Witwicka et al PLoS One. 2015 Jun 4;10(6):e0128275. doi

   Insert

   OC-STAMP (Ocstamp Mouse)
   

   Use

   Tags

   3x Flag and c-Myc tag

   Expression

   Mammalian

   Mutation

   changed asparagin (N) 162 to Aspartic Acid (D)

   Promoter

   Available since

   March 14, 2016

   Availability

   Academic Institutions and Nonprofits only

5. pUCmini-iCAP-PHP.V1

   Plasmid

   #127847

   Purpose

   non-standard AAV2 rep-AAV-PHP.V1 cap plasmid with AAV cap expression controlled by a tTA-TRE amplification system

   Depositor

   Viviana Gradinaru

   Article

   Ravindra Kumar et al Nat Methods. 2020 May;17(5):541-550. doi

   Insert

   Synthetic construct isolate AAV-PHP.V1 VP1 gene
   

   Use

   AAV

   Tags

   Expression

   Mammalian

   Mutation

   Promoter

   p41

   Available since

   April 21, 2020

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

6. pUCmini-iCAP-PHP.N

   Plasmid

   #127851

   Purpose

   non-standard AAV2 rep-AAV-PHP.N cap plasmid with AAV cap expression controlled by a tTA-TRE amplification system

   Depositor

   Viviana Gradinaru

   Article

   Ravindra Kumar et al Nat Methods. 2020 May;17(5):541-550. doi

   Insert

   Synthetic construct isolate AAV-PHP.N VP1 gene
   

   Use

   AAV

   Tags

   Expression

   Mammalian

   Mutation

   Promoter

   p41

   Available since

   April 21, 2020

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

7. pCJ136

   Plasmid

   #162665

   Purpose

   pET-21b(+) based plasmid for expression of MHETase from Ideonella sakaiensis 201-F6 (Genbank GAP38911.1), codon optimized for expression in E. coli K12, with C-terminal His tag.

   Depositor

   Gregg Beckham

   Article

   Knott et al Proc Natl Acad Sci U S A. 2020 Oct 13;11

   Insert

   MHETase from Ideonella sakaiensis 201-F6 (Genbank GAP38911.1)
   

   Use

   Tags

   His

   Expression

   Bacterial

   Mutation

   codon optimized for expression in E. coli K12

   Promoter

   T7/lac

   Available since

   Jan. 7, 2021

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

8. Rb-379-928-pGex2TK

   Plasmid

   #21749

   Depositor

   William Kaelin

   Article

   Kaelin et al Cell. 1992 Jul 24. 70(2):351-64.

   Insert

   Rb (RB1 Human)
   

   Use

   Tags

   GST

   Expression

   Bacterial

   Mutation

   deletion mutant ORF contains aa 379-928 of wt Rb …

   Promoter

   Available since

   Oct. 5, 2009

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

9. pGLS3-5xHRE-p53(K164R)

   Plasmid

   #72555

   Purpose

   Hypoxia induced mutant p53. Contains the HRE from VEGF sequence (5 repeats) upstream of p53 K164R.

   Depositor

   Ester Hammond

   Article

   Leszczynska et al J Clin Invest. 2015 Jun;125(6):2385-98.

   Insert

   p53 (TP53 Human)
   

   Use

   Tags

   5XHRE

   Expression

   Mammalian

   Mutation

   K164R

   Promoter

   E1B minimal promoter

   Available since

   March 9, 2017

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

10. pGLS3-5xHRE-p53(K120R)

    Plasmid

    #72554

    Purpose

    Hypoxia induced mutant p53. Contains the HRE from VEGF sequence (5 repeats) upstream of p53 K120R.

    Depositor

    Ester Hammond

    Article

    Leszczynska et al J Clin Invest. 2015 Jun;125(6):2385-98.

    Insert

    p53 (TP53 Human)
    

    Use

    Tags

    5XHRE

    Expression

    Mammalian

    Mutation

    K120R

    Promoter

    E1B minimal promoter

    Available since

    April 12, 2017

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

11. FUW-TetO-Gata6

    Plasmid

    #128827

    Purpose

    dox-dependent expression of mouse Gata6

    Depositor

    Yossi Buganim

    Article

    Benchetrit et al Cell Stem Cell. 2019 Jun 6;24(6):983-994

    Insert

    Gata6 (Gata6 Mouse)
    

    Use

    Lentiviral

    Tags

    Expression

    Mutation

    Promoter

    Available since

    Aug. 20, 2019

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

12. pUCmini-iCAP-PHP.V2

    Plasmid

    #127848

    Purpose

    non-standard AAV2 rep-AAV-PHP.V2 cap plasmid with AAV cap expression controlled by a tTA-TRE amplification system

    Depositor

    Viviana Gradinaru

    Article

    Ravindra Kumar et al Nat Methods. 2020 May;17(5):541-550. doi

    Insert

    Synthetic construct isolate AAV-PHP.V2 VP1 gene
    

    Use

    AAV

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    p41

    Available since

    April 21, 2020

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

13. pUCmini-iCAP-PHP.B4

    Plasmid

    #127849

    Purpose

    non-standard AAV2 rep-AAV-PHP.B4 cap plasmid with AAV cap expression controlled by a tTA-TRE amplification system

    Depositor

    Viviana Gradinaru

    Article

    Ravindra Kumar et al Nat Methods. 2020 May;17(5):541-550. doi

    Insert

    Synthetic construct isolate AAV-PHP.B4 VP1 gene
    

    Use

    AAV

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    p41

    Available since

    April 21, 2020

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

14. FCM101

    Plasmid

    #194298

    Purpose

    E. coli expression vector for monobody adaptor, FCM101, that bind to the Fc region of mouse IgG1 subclass. It contains a single Cys residue at the C-terminus for chemical conjugation, N-terminal His6

    Depositor

    Shohei Koide

    Article

    Albert et al Nat Commun. 2022 Oct 11;13(1):5998. doi:

    Insert

    FCM101
    

    Use

    Tags

    His tag, FLAG tag

    Expression

    Bacterial

    Mutation

    Promoter

    T7

    Available since

    March 31, 2023

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

15. pCJ207

    Plasmid

    #162680

    Purpose

    pET-21b(+) based plasmid for expression of the putative MHETase from Hydrogenophaga sp. PML113 (Genbank WP_083293388.1) with C-terminal His tag, codon optimized for expression in E. coli K12.

    Depositor

    Gregg Beckham

    Article

    Knott et al Proc Natl Acad Sci U S A. 2020 Oct 13;11

    Insert

    Putative MHETase from Hydrogenophaga sp. PML113 (Genbank WP_083293388.1) with signal peptide
    

    Use

    Tags

    His

    Expression

    Bacterial

    Mutation

    codon optimized for expression in E. coli K12

    Promoter

    T7/lac

    Available since

    Jan. 7, 2021

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

16. pX330-Elf5 gRNA

    Plasmid

    #128836

    Purpose

    gRNA for targeting mouse Elf5 loci using CRISPR-cas technique

    Depositor

    Yossi Buganim

    Article

    Benchetrit et al Cell Stem Cell. 2019 Jun 6;24(6):983-994

    Insert

    mElf5 gRNA (Elf5 Mouse)
    

    Use

    CRISPR

    Tags

    Expression

    Mutation

    Promoter

    Available since

    Aug. 12, 2019

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

17. FUW-TetO-Klf5

    Plasmid

    #128830

    Purpose

    dox-dependent expression of mouse Klf5

    Depositor

    Yossi Buganim

    Article

    Benchetrit et al Cell Stem Cell. 2019 Jun 6;24(6):983-994

    Insert

    Klf5 (Klf5 Mouse)
    

    Use

    Lentiviral

    Tags

    Expression

    Mutation

    Promoter

    Available since

    Aug. 9, 2019

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

18. scAAV-TTR-mFgf15

    Plasmid

    #190594

    Purpose

    AAV construct containing Fgf15 cds under control of TTR promoter

    Depositor

    Roel Nusse

    Article

    Sarkar et al Elife. 2023 Jan 31;12:e82311. doi: 10.75

    Insert

    Fgf15 (Fgf15 Mouse)
    

    Use

    AAV

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    Ttr

    Available since

    Feb. 13, 2023

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

19. pNTKV- Esrrb-KI-3'UTR-2A-TagBFP-RBGpA

    Plasmid

    #128835

    Purpose

    Knock-in of TagBFP into mouse Esrrb locus

    Depositor

    Yossi Buganim

    Article

    Benchetrit et al Cell Stem Cell. 2019 Jun 6;24(6):983-994

    Insert

    tagBFP into Esrrb locus (Esrrb Mouse)
    

    Use

    Mouse Targeting

    Tags

    Expression

    Mutation

    Promoter

    Available since

    Aug. 21, 2019

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

20. pX330-Esrrb gRNA

    Plasmid

    #128841

    Purpose

    gRNA for targeting mouse Esrrb locus using CRISPR-cas technique

    Depositor

    Yossi Buganim

    Article

    Benchetrit et al Cell Stem Cell. 2019 Jun 6;24(6):983-994

    Insert

    Esrrb gRNA (Esrrb Mouse)
    

    Use

    CRISPR

    Tags

    Expression

    Mutation

    Promoter

    Available since

    Aug. 27, 2019

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

21. pDONR221_SLC3A1

    Plasmid

    #132201

    Purpose

    Gateway entry clone with codon-optimized ORF sequence for subcloning into custom expression vectors

    Depositor

    Giulio Superti-Furga, RESOLUTE Consortium

    Article

    Superti-Furga et al Nat Rev Drug Discov. 2020 Jul;19(7):429-

    Insert

    SLC3A1 (SLC3A1 Human)
    

    Use

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    Available since

    Oct. 28, 2019

    Availability

    Industry, Academic Institutions, and Nonprofits

    

    Enlarge

22. EF1a-FXN-P2A-mRuby2-bGH

    Plasmid

    #235300

    Purpose

    ComMAND base gene regulating FXN-P2A-mRuby2 (therapeutically relevant gene)

    Depositor

    Kate Galloway

    Article

    Love et al Cell Syst. 2025 Apr 22:101269. doi: 10.1

    Insert

    FXN-P2A-mRuby2 (FXN Human)
    

    Use

    Synthetic Biology

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    EF1a (human EF1a)

    Available since

    May 30, 2025

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

23. EF1a-EGFP(miRE.FF4)-FMRP-TS.FF6x1-bGH

    Plasmid

    #235266

    Purpose

    ComMAND EGFP-FMRP open-loop circuit

    Depositor

    Kate Galloway

    Article

    Love et al Cell Syst. 2025 Apr 22:101269. doi: 10.1

    Insert

    EGFP-Fmr1 (Fmr1 Mouse)
    

    Use

    Synthetic Biology

    Tags

    FLAG

    Expression

    Mammalian

    Mutation

    Promoter

    EF1a (human EF1a)

    Available since

    May 14, 2025

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

24. EF1a-EGFP(miRE.FF4)-FMRP-TS.FF4x1-bGH

    Plasmid

    #235267

    Purpose

    ComMAND EGFP-FMRP closed-loop circuit

    Depositor

    Kate Galloway

    Article

    Love et al Cell Syst. 2025 Apr 22:101269. doi: 10.1

    Insert

    EGFP-Fmr1 (Fmr1 Mouse)
    

    Use

    Synthetic Biology

    Tags

    FLAG

    Expression

    Mammalian

    Mutation

    Promoter

    EF1a (human EF1a)

    Available since

    May 14, 2025

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

25. px335 Slc1a3 nickase 1

    Plasmid

    #129410

    Purpose

    Cas9n (nickase) with sgRNA directed to the terminal exon of mouse Slc1a3 gene

    Depositor

    Walker Jackson

    Article

    Kaczmarczyk et al Sci Rep. 2021 Mar 8;11(1):5412. doi: 10.

    Insert

    sgRNA directing Cas9n to the proximity of Slc1a3 STOP codon (Slc1a3 Mouse)
    

    Use

    CRISPR and Mouse Targeting

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    Available since

    May 2, 2022

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

26. ACA8-mYFP

    Plasmid

    #87245

    Purpose

    fluorescent labelling of ACA8

    Depositor

    Silke Robatzek

    Article

    Frei dit Frey et al Plant Physiol. 2012 Jun;159(2):798-809.

    Insert

    ACA8 (ACA8 Mustard Weed)
    

    Use

    Tags

    YFP

    Expression

    Mutation

    Promoter

    35s

    Available since

    April 25, 2017

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

27. ACA8-YFPc

    Plasmid

    #87243

    Purpose

    fluorescence complementation assay

    Depositor

    Silke Robatzek

    Article

    Frei dit Frey et al Plant Physiol. 2012 Jun;159(2):798-809.

    Insert

    ACA8 (ACA8 Mustard Weed)
    

    Use

    Tags

    YFPc

    Expression

    Mutation

    Promoter

    35s

    Available since

    April 25, 2017

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

28. ACA8-YFPn

    Plasmid

    #87241

    Purpose

    fluorescence complementation assay

    Depositor

    Silke Robatzek

    Article

    Frei dit Frey et al Plant Physiol. 2012 Jun;159(2):798-809.

    Insert

    ACA8 (ACA8 Mustard Weed)
    

    Use

    Tags

    YFPn

    Expression

    Mutation

    Promoter

    35s

    Available since

    April 25, 2017

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

29. pCJ202

    Plasmid

    #162675

    Purpose

    pET-21b(+) based plasmid for expression of PETase from Ideonella sakaiensis 201-F6 (Genbank GAP38373.1), codon optimized for expression in E. coli K12, with C-terminal His tag, incorporating W159C and S238C mutations.

    Depositor

    Gregg Beckham

    Article

    Knott et al Proc Natl Acad Sci U S A. 2020 Oct 13;11

    Insert

    PETase from Ideonella sakaiensis 201-F6 (Genbank GAP38373.1)
    

    Use

    Tags

    His

    Expression

    Bacterial

    Mutation

    W159C, S238C; codon optimized for expression in E…

    Promoter

    T7/lac

    Available since

    Feb. 3, 2021

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

30. pOT_4 - lenti-EFS-FMC6.3-BBz-2A-puro-2A-LTBR

    Plasmid

    #181973

    Purpose

    Expresses anti-CD19 CAR (with 4-1BB signaling domain) linked to puromycin resistance via P2A and to human LTBR via T2A for lentiviral delivery.

    Depositor

    Neville Sanjana

    Article

    Legut et al Nature. 2022 Mar;603(7902):728-735. doi:

    Insert

    FMC6.3-BBz CAR; LTBR (LTBR Human, Synthetic)
    

    Use

    Lentiviral

    Tags

    Expression

    Mutation

    Promoter

    Available since

    April 25, 2022

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

31. pOT_3 - lenti-EFS-FMC6.3-28z-2A-puro-2A-LTBR

    Plasmid

    #181972

    Purpose

    Expresses anti-CD19 CAR (with CD28 signaling domain) linked to puromycin resistance via P2A and to human LTBR via T2A for lentiviral delivery.

    Depositor

    Neville Sanjana

    Article

    Legut et al Nature. 2022 Mar;603(7902):728-735. doi:

    Insert

    FMC6.3-28z CAR; LTBR (LTBR Human, Synthetic)
    

    Use

    Lentiviral

    Tags

    Expression

    Mutation

    Promoter

    Available since

    April 25, 2022

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

32. pNTKV- Elf5-KI-3'UTR-2A-EYFP-NLS

    Plasmid

    #128833

    Purpose

    Knock-in of nuclear EYFP into mouse Elf5 locus

    Depositor

    Yossi Buganim

    Article

    Benchetrit et al Cell Stem Cell. 2019 Jun 6;24(6):983-994

    Insert

    Nuclear EYFP into Elf5 locus (Elf5 Mouse)
    

    Use

    Mouse Targeting

    Tags

    Expression

    Mutation

    Promoter

    Available since

    Aug. 9, 2019

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

33. pGLS3-5xHRE-p53(K120R/K164R)

    Plasmid

    #72556

    Purpose

    Hypoxia induced mutant p53. Contains the HRE from VEGF sequence (5 repeats) upstream of p53 K120R/K164R.

    Depositor

    Ester Hammond

    Article

    Leszczynska et al J Clin Invest. 2015 Jun;125(6):2385-98.

    Insert

    p53 (TP53 Human)
    

    Use

    Tags

    5XHRE

    Expression

    Mammalian

    Mutation

    K120R/K164R

    Promoter

    E1B minimal promoter

    Available since

    March 15, 2017

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

34. pCJ199

    Plasmid

    #162672

    Purpose

    pET-21b(+) based plasmid for expression of the putative MHET hydrolase from Comamonas thiooxydans (Genbank WP_080747404.1) with C-terminal His tag, codon optimized for expression in E. coli K12.

    Depositor

    Gregg Beckham

    Article

    Knott et al Proc Natl Acad Sci U S A. 2020 Oct 13;11

    Insert

    Putative MHET hydrolase from Comamonas thiooxydans (Genbank WP_080747404.1) with signal peptide
    

    Use

    Tags

    His

    Expression

    Bacterial

    Mutation

    codon optimized for expression in E. coli K12

    Promoter

    T7/lac

    Available since

    Jan. 7, 2021

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

35. pCJ197

    Plasmid

    #162670

    Purpose

    pET-21b(+) based plasmid for expression of MHETase from Ideonella sakaiensis 201-F6 (Genbank GAP38911.1), codon optimized for expression in E. coli K12, with C-terminal His tag, incorporating S131G mutation.

    Depositor

    Gregg Beckham

    Article

    Knott et al Proc Natl Acad Sci U S A. 2020 Oct 13;11

    Insert

    MHETase from Ideonella sakaiensis 201-F6 (Genbank GAP38911.1
    

    Use

    Tags

    His

    Expression

    Bacterial

    Mutation

    S131G; codon optimized for expression in E. coli …

    Promoter

    T7/lac

    Available since

    Jan. 7, 2021

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

36. pCJ203

    Plasmid

    #162678

    Purpose

    pET-21b(+) based plasmid for expression of the putative MHET hydrolase from Comomonas thiooxydans (Genbank WP_080747404.1) with deletion of the predicted signal peptide (Phe2:Ala75)

    Depositor

    Gregg Beckham

    Article

    Knott et al Proc Natl Acad Sci U S A. 2020 Oct 13;11

    Insert

    Putative MHETase from Comomonas thiooxydans (Genbank WP_080747404.1) without 75 residue signal peptide
    

    Use

    Tags

    His

    Expression

    Bacterial

    Mutation

    deltaF2-A75; codon optimized for expression in E.…

    Promoter

    T7/lac

    Available since

    Feb. 3, 2021

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

37. EF1a-FXN-P2A-mRuby2(miRE.FF4)-TS.FF6x1-bGH

    Plasmid

    #235301

    Purpose

    ComMAND open-loop circuit regulating FXN-P2A-mRuby2 (therapeutically relevant gene)

    Depositor

    Kate Galloway

    Article

    Love et al Cell Syst. 2025 Apr 22:101269. doi: 10.1

    Insert

    FXN-P2A-mRuby2 (FXN Human)
    

    Use

    Synthetic Biology

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    EF1a (human EF1a)

    Available since

    June 11, 2025

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

38. EF1a-FXN-P2A-mRuby2(miRE.FF4)-TS.FF4x1-bGH

    Plasmid

    #235302

    Purpose

    ComMAND closed-loop circuit regulating FXN-P2A-mRuby2 (therapeutically relevant gene)

    Depositor

    Kate Galloway

    Article

    Love et al Cell Syst. 2025 Apr 22:101269. doi: 10.1

    Insert

    FXN-P2A-mRuby2 (FXN Human)
    

    Use

    Synthetic Biology

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    EF1a (human EF1a)

    Available since

    May 30, 2025

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

39. pRSF_his6_sumo_ZIKV_NS5_MTase

    Plasmid

    #213529

    Purpose

    Expresses the MTase domain of NS5 of ZIKV

    Depositor

    Thomas Tuschl

    Article

    Meyer et al Nature. 2024 Dec 11. doi: 10.1038/s41586

    Insert

    ZIKA virus NS5 methyltransferase domain
    

    Use

    Tags

    His6-sumo

    Expression

    Bacterial

    Mutation

    MTase domain aa 1-265

    Promoter

    Available since

    Dec. 12, 2024

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

40. pCJ205

    Plasmid

    #162676

    Purpose

    pET-21b(+) based plasmid for expression of MHETase from Ideonella sakaiensis 201-F6 (Genbank GAP38911.1), codon optimized for expression in E. coli K12, with C-terminal His tag, incorporating C224A an

    Depositor

    Gregg Beckham

    Article

    Knott et al Proc Natl Acad Sci U S A. 2020 Oct 13;11

    Insert

    MHETase from Ideonella sakaiensis 201-F6 (Genbank GAP38911.1)
    

    Use

    Tags

    His

    Expression

    Bacterial

    Mutation

    C224A, C529A; codon optimized for expression in E…

    Promoter

    T7/lac

    Available since

    Feb. 3, 2021

    Availability

    Academic Institutions and Nonprofits only

    

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