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Showing: 901 - 950 of 11158 results
  1. pLKO_HSV.V5.HA_NGFR

    Plasmid
    #158290
    Purpose
    Protein Barcode (Pro-Code) for vector/cell tracking. Contains U6 trRNA cassette for sgRNA cloning. Enables phenotypic CRISPR screens at a single cell resolution (using cytometry).
    Depositor
    Insert
    Pro-Code Tagged human dNGFR (NGFR Human)
    Use
    CRISPR and Lentiviral
    Tags
    HSV.V5.HA
    Expression
    Mutation
    Truncation of the signaling domain
    Promoter
    EF1a
    Available since
    Sept. 3, 2021
    Availability
    Academic Institutions and Nonprofits only
  2. pLKO_HSV.AU1.V5_NGFR

    Plasmid
    #158291
    Purpose
    Protein Barcode (Pro-Code) for vector/cell tracking. Contains U6 trRNA cassette for sgRNA cloning. Enables phenotypic CRISPR screens at a single cell resolution (using cytometry).
    Depositor
    Insert
    Pro-Code Tagged human dNGFR (NGFR Human)
    Use
    CRISPR and Lentiviral
    Tags
    HSV.AU1.V5
    Expression
    Mutation
    Truncation of the signaling domain
    Promoter
    EF1a
    Available since
    Sept. 3, 2021
    Availability
    Academic Institutions and Nonprofits only
  3. pLKO_HSV.V5.VSVg_NGFR

    Plasmid
    #158292
    Purpose
    Protein Barcode (Pro-Code) for vector/cell tracking. Contains U6 trRNA cassette for sgRNA cloning. Enables phenotypic CRISPR screens at a single cell resolution (using cytometry).
    Depositor
    Insert
    Pro-Code Tagged human dNGFR (NGFR Human)
    Use
    CRISPR and Lentiviral
    Tags
    HSV.V5.VSVg
    Expression
    Mutation
    Truncation of the signaling domain
    Promoter
    EF1a
    Available since
    Sept. 3, 2021
    Availability
    Academic Institutions and Nonprofits only
  4. pLKO_HSV.AU1.FLAG_NGFR

    Plasmid
    #158295
    Purpose
    Protein Barcode (Pro-Code) for vector/cell tracking. Contains U6 trRNA cassette for sgRNA cloning. Enables phenotypic CRISPR screens at a single cell resolution (using cytometry).
    Depositor
    Insert
    Pro-Code Tagged human dNGFR (NGFR Human)
    Use
    CRISPR and Lentiviral
    Tags
    HSV.AU1.FLAG
    Expression
    Mutation
    Truncation of the signaling domain
    Promoter
    EF1a
    Available since
    Sept. 3, 2021
    Availability
    Academic Institutions and Nonprofits only
  5. pLKO_HSV.AU1.NWS_NGFR

    Plasmid
    #158298
    Purpose
    Protein Barcode (Pro-Code) for vector/cell tracking. Contains U6 trRNA cassette for sgRNA cloning. Enables phenotypic CRISPR screens at a single cell resolution (using cytometry).
    Depositor
    Insert
    Pro-Code Tagged human dNGFR (NGFR Human)
    Use
    CRISPR and Lentiviral
    Tags
    HSV.AU1.NWS
    Expression
    Mutation
    Truncation of the signaling domain
    Promoter
    EF1a
    Available since
    Sept. 3, 2021
    Availability
    Academic Institutions and Nonprofits only
  6. pLKO_HSV.AU1.HA_NGFR

    Plasmid
    #158300
    Purpose
    Protein Barcode (Pro-Code) for vector/cell tracking. Contains U6 trRNA cassette for sgRNA cloning. Enables phenotypic CRISPR screens at a single cell resolution (using cytometry).
    Depositor
    Insert
    Pro-Code Tagged human dNGFR (NGFR Human)
    Use
    CRISPR and Lentiviral
    Tags
    HSV.AU1.HA
    Expression
    Mutation
    Truncation of the signaling domain
    Promoter
    EF1a
    Available since
    Sept. 3, 2021
    Availability
    Academic Institutions and Nonprofits only
  7. pLKO_HSV.AU1.VSVg_NGFR

    Plasmid
    #158302
    Purpose
    Protein Barcode (Pro-Code) for vector/cell tracking. Contains U6 trRNA cassette for sgRNA cloning. Enables phenotypic CRISPR screens at a single cell resolution (using cytometry).
    Depositor
    Insert
    Pro-Code Tagged human dNGFR (NGFR Human)
    Use
    CRISPR and Lentiviral
    Tags
    HSV.AU1.VSVg
    Expression
    Mutation
    Truncation of the signaling domain
    Promoter
    EF1a
    Available since
    Sept. 3, 2021
    Availability
    Academic Institutions and Nonprofits only
  8. pLKO_Tag.V5.S.Ollas_NGFR

    Plasmid
    #158304
    Purpose
    Protein Barcode (Pro-Code) for vector/cell tracking. Contains U6 trRNA cassette for sgRNA cloning. Enables phenotypic CRISPR screens at a single cell resolution (using cytometry).
    Depositor
    Insert
    Pro-Code Tagged human dNGFR (NGFR Human)
    Use
    CRISPR and Lentiviral
    Tags
    Tag.V5.S.Ollas
    Expression
    Mutation
    Truncation of the signaling domain
    Promoter
    EF1a
    Available since
    Sept. 3, 2021
    Availability
    Academic Institutions and Nonprofits only
  9. pLKO_Tag.AU1.S.Ollas_NGFR

    Plasmid
    #158308
    Purpose
    Protein Barcode (Pro-Code) for vector/cell tracking. Contains U6 trRNA cassette for sgRNA cloning. Enables phenotypic CRISPR screens at a single cell resolution (using cytometry).
    Depositor
    Insert
    Pro-Code Tagged human dNGFR (NGFR Human)
    Use
    CRISPR and Lentiviral
    Tags
    Tag.AU1.S.Ollas
    Expression
    Mutation
    Truncation of the signaling domain
    Promoter
    EF1a
    Available since
    Sept. 3, 2021
    Availability
    Academic Institutions and Nonprofits only
  10. pLKO_HA.V5.C_NGFR

    Plasmid
    #158262
    Purpose
    Protein Barcode (Pro-Code) for vector/cell tracking. Contains U6 trRNA cassette for sgRNA cloning. Enables phenotypic CRISPR screens at a single cell resolution (using cytometry).
    Depositor
    Insert
    Pro-Code Tagged human dNGFR (NGFR Human)
    Use
    CRISPR and Lentiviral
    Tags
    HA.V5.C
    Expression
    Mutation
    Truncation of the signaling domain
    Promoter
    EF1a
    Available since
    Sept. 3, 2021
    Availability
    Academic Institutions and Nonprofits only
  11. pLKO_NWS.V5.C_NGFR

    Plasmid
    #158264
    Purpose
    Protein Barcode (Pro-Code) for vector/cell tracking. Contains U6 trRNA cassette for sgRNA cloning. Enables phenotypic CRISPR screens at a single cell resolution (using cytometry).
    Depositor
    Insert
    Pro-Code Tagged human dNGFR (NGFR Human)
    Use
    CRISPR and Lentiviral
    Tags
    NWS.V5.C
    Expression
    Mutation
    Truncation of the signaling domain
    Promoter
    EF1a
    Available since
    Sept. 3, 2021
    Availability
    Academic Institutions and Nonprofits only
  12. pLKO_V5.VSVg.C_NGFR

    Plasmid
    #158266
    Purpose
    Protein Barcode (Pro-Code) for vector/cell tracking. Contains U6 trRNA cassette for sgRNA cloning. Enables phenotypic CRISPR screens at a single cell resolution (using cytometry).
    Depositor
    Insert
    Pro-Code Tagged human dNGFR (NGFR Human)
    Use
    CRISPR and Lentiviral
    Tags
    V5.VSVg.C
    Expression
    Mutation
    Truncation of the signaling domain
    Promoter
    EF1a
    Available since
    Sept. 3, 2021
    Availability
    Academic Institutions and Nonprofits only
  13. pLKO_HSV.AU1.Ollas_NGFR

    Plasmid
    #158273
    Purpose
    Protein Barcode (Pro-Code) for vector/cell tracking. Contains U6 trRNA cassette for sgRNA cloning. Enables phenotypic CRISPR screens at a single cell resolution (using cytometry).
    Depositor
    Insert
    Pro-Code Tagged human dNGFR (NGFR Human)
    Use
    CRISPR and Lentiviral
    Tags
    HSV.AU1.Ollas
    Expression
    Mutation
    Truncation of the signaling domain
    Promoter
    EF1a
    Available since
    Sept. 3, 2021
    Availability
    Academic Institutions and Nonprofits only
  14. pLKO_NWS.AU1.FLAG_NGFR

    Plasmid
    #158238
    Purpose
    Protein Barcode (Pro-Code) for vector/cell tracking. Contains U6 trRNA cassette for sgRNA cloning. Enables phenotypic CRISPR screens at a single cell resolution (using cytometry).
    Depositor
    Insert
    Pro-Code Tagged human dNGFR (NGFR Human)
    Use
    CRISPR and Lentiviral
    Tags
    NWS.AU1.FLAG
    Expression
    Mutation
    Truncation of the signaling domain
    Promoter
    EF1a
    Available since
    Sept. 3, 2021
    Availability
    Academic Institutions and Nonprofits only
  15. pLKO_AU1.FLAG.VSVg_NGFR

    Plasmid
    #158242
    Purpose
    Protein Barcode (Pro-Code) for vector/cell tracking. Contains U6 trRNA cassette for sgRNA cloning. Enables phenotypic CRISPR screens at a single cell resolution (using cytometry).
    Depositor
    Insert
    Pro-Code Tagged human dNGFR (NGFR Human)
    Use
    CRISPR and Lentiviral
    Tags
    AU1.FLAG.VSVg
    Expression
    Mutation
    Truncation of the signaling domain
    Promoter
    EF1a
    Available since
    Sept. 3, 2021
    Availability
    Academic Institutions and Nonprofits only
  16. pLKO_FLAG.AU1.C_NGFR

    Plasmid
    #158252
    Purpose
    Protein Barcode (Pro-Code) for vector/cell tracking. Contains U6 trRNA cassette for sgRNA cloning. Enables phenotypic CRISPR screens at a single cell resolution (using cytometry).
    Depositor
    Insert
    Pro-Code Tagged human dNGFR (NGFR Human)
    Use
    CRISPR and Lentiviral
    Tags
    FLAG.AU1.C
    Expression
    Mutation
    Truncation of the signaling domain
    Promoter
    EF1a
    Available since
    Sept. 3, 2021
    Availability
    Academic Institutions and Nonprofits only
  17. pLKO_HA.AU1.C_NGFR

    Plasmid
    #158253
    Purpose
    Protein Barcode (Pro-Code) for vector/cell tracking. Contains U6 trRNA cassette for sgRNA cloning. Enables phenotypic CRISPR screens at a single cell resolution (using cytometry).
    Depositor
    Insert
    Pro-Code Tagged human dNGFR (NGFR Human)
    Use
    CRISPR and Lentiviral
    Tags
    HA.AU1.C
    Expression
    Mutation
    Truncation of the signaling domain
    Promoter
    EF1a
    Available since
    Sept. 3, 2021
    Availability
    Academic Institutions and Nonprofits only
  18. pLKO_NWS.AU1.C_NGFR

    Plasmid
    #158254
    Purpose
    Protein Barcode (Pro-Code) for vector/cell tracking. Contains U6 trRNA cassette for sgRNA cloning. Enables phenotypic CRISPR screens at a single cell resolution (using cytometry).
    Depositor
    Insert
    Pro-Code Tagged human dNGFR (NGFR Human)
    Use
    CRISPR and Lentiviral
    Tags
    NWS.AU1.C
    Expression
    Mutation
    Truncation of the signaling domain
    Promoter
    EF1a
    Available since
    Sept. 3, 2021
    Availability
    Academic Institutions and Nonprofits only
  19. pLKO_V5.AU1.C_NGFR

    Plasmid
    #158255
    Purpose
    Protein Barcode (Pro-Code) for vector/cell tracking. Contains U6 trRNA cassette for sgRNA cloning. Enables phenotypic CRISPR screens at a single cell resolution (using cytometry).
    Depositor
    Insert
    Pro-Code Tagged human dNGFR (NGFR Human)
    Use
    CRISPR and Lentiviral
    Tags
    V5.AU1.C
    Expression
    Mutation
    Truncation of the signaling domain
    Promoter
    EF1a
    Available since
    Sept. 3, 2021
    Availability
    Academic Institutions and Nonprofits only
  20. pLKO_VSVg.AU1.C_NGFR

    Plasmid
    #158256
    Purpose
    Protein Barcode (Pro-Code) for vector/cell tracking. Contains U6 trRNA cassette for sgRNA cloning. Enables phenotypic CRISPR screens at a single cell resolution (using cytometry).
    Depositor
    Insert
    Pro-Code Tagged human dNGFR (NGFR Human)
    Use
    CRISPR and Lentiviral
    Tags
    VSVg.AU1.C
    Expression
    Mutation
    Truncation of the signaling domain
    Promoter
    EF1a
    Available since
    Sept. 3, 2021
    Availability
    Academic Institutions and Nonprofits only
  21. pLKO_FLAG.V5.C_NGFR

    Plasmid
    #158259
    Purpose
    Protein Barcode (Pro-Code) for vector/cell tracking. Contains U6 trRNA cassette for sgRNA cloning. Enables phenotypic CRISPR screens at a single cell resolution (using cytometry).
    Depositor
    Insert
    Pro-Code Tagged human dNGFR (NGFR Human)
    Use
    CRISPR and Lentiviral
    Tags
    FLAG.V5.C
    Expression
    Mutation
    Truncation of the signaling domain
    Promoter
    EF1a
    Available since
    Sept. 3, 2021
    Availability
    Academic Institutions and Nonprofits only
  22. pLKO_HA.AU1.NWS_NGFR

    Plasmid
    #158234
    Purpose
    Protein Barcode (Pro-Code) for vector/cell tracking. Contains U6 trRNA cassette for sgRNA cloning. Enables phenotypic CRISPR screens at a single cell resolution (using cytometry).
    Depositor
    Insert
    Pro-Code Tagged human dNGFR (NGFR Human)
    Use
    CRISPR and Lentiviral
    Tags
    HA.AU1.NWS
    Expression
    Mutation
    Truncation of the signaling domain
    Promoter
    EF1a
    Available since
    Sept. 3, 2021
    Availability
    Academic Institutions and Nonprofits only
  23. pLKO_HA.AU1.FLAG_NGFR

    Plasmid
    #158236
    Purpose
    Protein Barcode (Pro-Code) for vector/cell tracking. Contains U6 trRNA cassette for sgRNA cloning. Enables phenotypic CRISPR screens at a single cell resolution (using cytometry).
    Depositor
    Insert
    Pro-Code Tagged human dNGFR (NGFR Human)
    Use
    CRISPR and Lentiviral
    Tags
    HA.AU1.FLAG
    Expression
    Mutation
    Truncation of the signaling domain
    Promoter
    EF1a
    Available since
    Sept. 3, 2021
    Availability
    Academic Institutions and Nonprofits only
  24. pLKO_NWS.AU1.V5_NGFR

    Plasmid
    #158237
    Purpose
    Protein Barcode (Pro-Code) for vector/cell tracking. Contains U6 trRNA cassette for sgRNA cloning. Enables phenotypic CRISPR screens at a single cell resolution (using cytometry).
    Depositor
    Insert
    Pro-Code Tagged human dNGFR (NGFR Human)
    Use
    CRISPR and Lentiviral
    Tags
    NWS.AU1.V5
    Expression
    Mutation
    Truncation of the signaling domain
    Promoter
    EF1a
    Available since
    Sept. 3, 2021
    Availability
    Academic Institutions and Nonprofits only
  25. pcDNA3.1-nAChr-Alpha6-CFP

    Plasmid
    #50482
    Purpose
    this report is the first to directly measure nAChr subunit stoichiometry using FRET and plasma membrane localization of Alpha6 and Beta3 containing receptors using TIRF
    Depositor
    Insert
    nAChr-alpha6 (Chrna6 Mouse)
    Use
    Tags
    CFP fusion in M3-M4 loop (after residue A405)
    Expression
    Mammalian
    Mutation
    Gly-Ala-Gly flexible linker flanking the CFP open…
    Promoter
    CMV
    Available since
    Jan. 13, 2014
    Availability
    Academic Institutions and Nonprofits only
  26. pET28 His-MBP-TEV-BAP-Human BCKDK

    Plasmid
    #232116
    Purpose
    For bacterial expression of Human BCKDK (AA31–412, missing precursor peptide) with an N-terminal biotin acceptor peptide and a cleavable His-MBP purification tag.
    Depositor
    Insert
    BCKDK (BCKDK Human)
    Use
    Tags
    6 x His, Biotin acceptor peptide (BAP), Maltose-B…
    Expression
    Bacterial
    Mutation
    Promoter
    T7 Promoter
    Available since
    March 11, 2025
    Availability
    Academic Institutions and Nonprofits only
  27. pRP[CRISPR]-EGFP/Puro-hCas9-U6>(long left)-U6>(long right)

    Plasmid
    #216871
    Purpose
    This plasmid contains hCas9 and two gRNAs that can excise the genomic area around the mouse mdx mutation in dystrophin's exon 23
    Depositor
    Insert
    Cas9, gRNA Long.L, gRNA Long.R (Dmd Mouse)
    Use
    CRISPR
    Tags
    Expression
    Mutation
    mdx mutation in mouse dystrophin
    Promoter
    Available since
    June 3, 2024
    Availability
    Academic Institutions and Nonprofits only
  28. pET28 E2 LBD-Tev-E1a-His

    Plasmid
    #232117
    Purpose
    For bacterial expression of a fusion protein containing from N to C-terminus: the lipoyl-binding domain of Human E2, a TEV protease site, a peptide from Human E1a, and a His tag.
    Depositor
    Insert
    DBT (DBT Human)
    Use
    Tags
    6xHis, RIGHHSTSDDSSAY (AA331 to 345 (preprocessin…
    Expression
    Bacterial
    Mutation
    Promoter
    T7 Promoter
    Available since
    March 20, 2025
    Availability
    Academic Institutions and Nonprofits only
  29. pKT-IDH1(R132H)-IRES-Katushka

    Plasmid
    #124257
    Purpose
    Expresses IDH1 with a R132H mutation and katushka fluorescent reporter. Construct has inverted repeats to be used in Sleeping Beauty transposon system.
    Depositor
    Insert
    Isocitrate dehydrogenase 1 (R132H) (IDH1 Human)
    Use
    Tags
    Expression
    Mammalian
    Mutation
    Arginine 132 is mutated to Histidine. This mutati…
    Promoter
    Available since
    Sept. 16, 2019
    Availability
    Academic Institutions and Nonprofits only
  30. pGLU_111

    Plasmid
    #225792
    Purpose
    RS421 library variant 13463
    Depositor
    Insert
    13463_[Tn5]
    Use
    Synthetic Biology
    Tags
    Expression
    Mutation
    N/A
    Promoter
    Available since
    Sept. 27, 2024
    Availability
    Academic Institutions and Nonprofits only
  31. pGLU_75

    Plasmid
    #225777
    Purpose
    RS421 library variant 13463
    Depositor
    Insert
    13463_[abr]
    Use
    Synthetic Biology
    Tags
    Expression
    Mutation
    N/A
    Promoter
    Available since
    Sept. 27, 2024
    Availability
    Academic Institutions and Nonprofits only
  32. pGLU_93

    Plasmid
    #225807
    Purpose
    RS421 library variant 13463
    Depositor
    Insert
    13463_[Himar]
    Use
    Synthetic Biology
    Tags
    Expression
    Mutation
    N/A
    Promoter
    Available since
    Sept. 27, 2024
    Availability
    Academic Institutions and Nonprofits only
  33. BTV

    Plasmid
    #131589
    Purpose
    Plasmid for the targeting of BAC clone
    Depositor
    Insert
    Firefly Luciferase
    Use
    Tags
    Expression
    Mammalian
    Mutation
    Promoter
    Available since
    Oct. 22, 2019
    Availability
    Academic Institutions and Nonprofits only
  34. 1162BX-CMV-EGFP-bGHpolyA

    Plasmid
    #235171
    Purpose
    Expresses EGFP
    Depositor
    Insert
    EGFP
    Use
    Tags
    Expression
    Mammalian
    Mutation
    Promoter
    Available since
    May 9, 2025
    Availability
    Academic Institutions and Nonprofits only
  35. pSB4A3-PBAD-phzO

    Plasmid
    #165619
    Purpose
    Arabinose-inducible expression of phzO for conversion of phenazine-1-carboxylic acid to 2-hydroxyphenazine
    Depositor
    Insert
    phzO
    Use
    Synthetic Biology
    Tags
    Expression
    Bacterial
    Mutation
    Promoter
    PBAD
    Available since
    Oct. 6, 2022
    Availability
    Academic Institutions and Nonprofits only
  36. pJK275

    Plasmid
    #73363
    Purpose
    Encodes Crepis alpina delta-8 sphingolipid desaturase (SLD1)
    Depositor
    Insert
    delta-8 sphingolipid desaturase
    Use
    Tags
    Expression
    Yeast
    Mutation
    missing residues 1-6
    Promoter
    GAL1
    Available since
    March 10, 2016
    Availability
    Academic Institutions and Nonprofits only
  37. ecALDH

    Plasmid
    #67921
    Purpose
    Expresses aldehyde dehydrogenase from Escherichia coli, codon optimized for expression in E. coli
    Depositor
    Insert
    Escherichia coli aldehyde dehydrogenase (puuC Escherichia coli)
    Use
    Tags
    6x-His
    Expression
    Bacterial
    Mutation
    Promoter
    T7
    Available since
    Sept. 17, 2015
    Availability
    Academic Institutions and Nonprofits only
  38. pJK138

    Plasmid
    #73314
    Purpose
    Pas2p peroxisomal membrane targeting sequence
    Depositor
    Insert
    Pas2p (peroxisomal membrane targeting region)
    Use
    Tags
    His6 and c-myc
    Expression
    Yeast
    Mutation
    encodes residues 1-42
    Promoter
    AOX1
    Available since
    Feb. 26, 2016
    Availability
    Academic Institutions and Nonprofits only
  39. pJK126

    Plasmid
    #73337
    Purpose
    Encodes Mycobacterium tuberculosis FdxB (C-terminal flavoprotein reductase domain)
    Depositor
    Insert
    electron transfer flavoprotein
    Use
    Tags
    His6
    Expression
    Bacterial
    Mutation
    encodes residues 328-685
    Promoter
    T7
    Available since
    Feb. 26, 2016
    Availability
    Academic Institutions and Nonprofits only
  40. pJK125

    Plasmid
    #73336
    Purpose
    Encodes Mycobacterium tuberculosis FdxB (N-terminal fatty acid desaturase domain)
    Depositor
    Insert
    electron transfer flavoprotein
    Use
    Tags
    His6
    Expression
    Bacterial
    Mutation
    encodes residues 1-327
    Promoter
    T7
    Available since
    Feb. 23, 2016
    Availability
    Academic Institutions and Nonprofits only
  41. IDG_SCNN1D_OE_1

    Plasmid
    #161666
    Purpose
    Overexpresses SCNN1D
    Depositor
    Insert
    SCNN1D (SCNN1D Human)
    Use
    Tags
    V5
    Expression
    Mammalian
    Mutation
    Promoter
    Available since
    Availability
    Academic Institutions and Nonprofits only
Showing: 901 - 950 of 11158 results