We narrowed to 29,660 results for: REP

1. pcDNA3.1-StrepHA-SUMO2

   Plasmid

   #66867

   Purpose

   Mammalian expression of SUMO2 with a strep and an HA tag

   Depositor

   Ying Liu

   Article

   Bursomanno et al J Proteome Res. 2015 Apr 3;14(4):1687-99

   Insert

   SUMO2 (SUMO2 Human)
   

   Use

   Tags

   HA and Strep

   Expression

   Mammalian

   Mutation

   Promoter

   CMV

   Available since

   June 22, 2015

   Availability

   Academic Institutions and Nonprofits only

   

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2. mCh-KIF5B*-strep

   Plasmid

   #120164

   Purpose

   Expresses a chimera of mCherry (fluorescent tag), a KIF5B-based anterograde motor and streptavidin

   Depositor

   Juan Bonifacino

   Article

   Guardia et al PLoS Biol. 2019 May 17;17(5):e3000279. d

   Insert

   kinesin family member 5B (Kif5b Mouse)
   

   Use

   Tags

   Streptavidin and mCherry

   Expression

   Mammalian

   Mutation

   Truncated KIF5B: 1-555 aa

   Promoter

   CMV

   Available since

   July 30, 2019

   Availability

   Academic Institutions and Nonprofits only

   

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3. pcDNA3.1_ 2xFLAG-2xSTREP_FBXO22

   Plasmid

   #159127

   Purpose

   expresses FBXO22 in mammalian cells

   Depositor

   Michele Pagano

   Article

   Lignitto et al Cell. 2019 Jul 11;178(2):316-329.e18. do

   Insert

   FBXO22 (FBXO22 Human)
   

   Use

   Tags

   2X-FLAG _ 2X-STREP

   Expression

   Mammalian

   Mutation

   Promoter

   CMV

   Available since

   Sept. 18, 2020

   Availability

   Academic Institutions and Nonprofits only

   

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4. 7h sgRNA for 4-μHOM reporter

   Plasmid

   #113625

   Purpose

   sgRNA/CAS9 expression plasmid to induce a 3’ double-strand break in the 4-μHOM reporter at the edge of the microhomology.

   Depositor

   Jeremy Stark

   Article

   Bhargava et al Nat Commun. 2018 Jun 27;9(1):2484. doi:

   Insert

   7h sgRNA
   

   Use

   CRISPR

   Tags

   Expression

   Mammalian

   Mutation

   Promoter

   Available since

   Aug. 13, 2018

   Availability

   Academic Institutions and Nonprofits only

5. 7i sgRNA for 4-μHOM reporter

   Plasmid

   #113626

   Purpose

   sgRNA/CAS9 expression plasmid to induce a 3’ double-strand break in the 4-μHOM reporter 8 nts. upstream from the microhomology

   Depositor

   Jeremy Stark

   Article

   Bhargava et al Nat Commun. 2018 Jun 27;9(1):2484. doi:

   Insert

   7i sgRNA
   

   Use

   CRISPR

   Tags

   Expression

   Mammalian

   Mutation

   Promoter

   Available since

   Aug. 13, 2018

   Availability

   Academic Institutions and Nonprofits only

6. pXT131_TwinStrep-SUMO-ShTniQ

   Plasmid

   #135527

   Purpose

   Protein expression construct for ShTniQ

   Depositor

   Feng Zhang

   Article

   Strecker et al Science. 2019 Jul 5;365(6448):48-53. doi

   Insert

   ShTniQ
   

   Use

   Tags

   TwinStrep-SUMO

   Expression

   Bacterial

   Mutation

   Promoter

   Available since

   Dec. 18, 2019

   Availability

   Academic Institutions and Nonprofits only

   

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7. pXT129_TwinStrep-SUMO-ShTnsB

   Plasmid

   #135525

   Purpose

   Protein expression construct for ShTnsB

   Depositor

   Feng Zhang

   Article

   Strecker et al Science. 2019 Jul 5;365(6448):48-53. doi

   Insert

   ShTnsB
   

   Use

   Tags

   TwinStrep-SUMO

   Expression

   Bacterial

   Mutation

   Promoter

   Available since

   Dec. 18, 2019

   Availability

   Academic Institutions and Nonprofits only

   

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8. pCDNA4.TO-ORF64-2xNSTREP

   Plasmid

   #136224

   Purpose

   Expresses N-terminally strep tagged Kaposi's Sarcoma Associated Herpesvirus (KSHV) ORF64

   Depositor

   Britt Glaunsinger

   Article

   Davis et al Mol Cell. 2015 Jan 22;57(2):349-60. doi:

   Insert

   ORF64
   

   Use

   Tags

   Expression

   Mammalian

   Mutation

   Promoter

   CMV

   Available since

   Jan. 9, 2020

   Availability

   Academic Institutions and Nonprofits only

   

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9. pBM-strep-UCP1

   Plasmid

   #216207

   Purpose

   for expression of human strep-tagged UCP1 protein in BacMam system

   Depositor

   Lei Chen

   Article

   Kang et al Nature. 2023 Aug;620(7972):226-231. doi:

   Insert

   UCP1 (UCP1 Human)
   

   Use

   Tags

   strep

   Expression

   Mammalian

   Mutation

   Promoter

   Available since

   April 22, 2024

   Availability

   Academic Institutions and Nonprofits only

   

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10. SeeSaw Reporter (SSR2.0)

    Plasmid

    #50393

    Purpose

    SSR2.0 has two target sites in opposite orientation for generate only one cycle of repairing after I-SceI endonuclease expression.

    Depositor

    Pablo Huertas Sánchez

    Article

    Gomez-Cabello et al PLoS One. 2013 Oct 14;8(10):e77206. doi:

    Insert

    SSR2.0
    

    Use

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    CMV

    Available since

    Feb. 14, 2014

    Availability

    Academic Institutions and Nonprofits only

    

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11. pcDNA5FRT/TO-Strep/HA-UBE2J1

    Plasmid

    #124669

    Purpose

    Expresses UBE2J1 with N-terminal Strep-HA tag in mammalian cells

    Depositor

    Hemmo Meyer

    Article

    Koerver et al EMBO Rep. 2019 Aug 21:e48014. doi: 10.15

    Insert

    UBE2J1 (UBE2J1 Human)
    

    Use

    Tags

    2xStrep/HA

    Expression

    Mammalian

    Mutation

    Promoter

    CMV

    Available since

    Sept. 24, 2019

    Availability

    Academic Institutions and Nonprofits only

    

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12. pFsynW SYT1 reporter

    Plasmid

    #197275

    Purpose

    Encodes for viral expression of wild type Synaptotagmin 1 (SYT1) to synchronize its transport from the ER (RUSH system); visualized with HaloTag. Reporter only (no hook).

    Depositor

    Edwin Chapman

    Article

    Watson et al Elife. 2023 Feb 2;12:e82568. doi: 10.755

    Insert

    SYT1 and HaloTag (Syt1 Rat)
    

    Use

    Lentiviral

    Tags

    FLAG

    Expression

    Mutation

    N/A

    Promoter

    hSyn (human synapsin I promoter)

    Available since

    July 28, 2023

    Availability

    Academic Institutions and Nonprofits only

    

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13. pCDNA4.TO-ORF73-2xCSTREP

    Plasmid

    #136232

    Purpose

    Expresses C-terminally strep tagged Kaposi's Sarcoma Associated Herpesvirus (KSHV) ORF73

    Depositor

    Britt Glaunsinger

    Article

    Davis et al Mol Cell. 2015 Jan 22;57(2):349-60. doi:

    Insert

    ORF73
    

    Use

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    CMV

    Available since

    May 12, 2020

    Availability

    Academic Institutions and Nonprofits only

    

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14. pFsynW SYB2 reporter

    Plasmid

    #197276

    Purpose

    Encodes for viral expression of wild type Synaptobrevin 2 (SYB2) to synchronize its transport from the ER (RUSH system); visualized with HaloTag. Reporter only (no hook).

    Depositor

    Edwin Chapman

    Article

    Watson et al Elife. 2023 Feb 2;12:e82568. doi: 10.755

    Insert

    SYB2 and HaloTag (Vamp2 Rat)
    

    Use

    Lentiviral

    Tags

    FLAG

    Expression

    Mutation

    N/A

    Promoter

    hSyn (human synapsin I promoter)

    Available since

    July 28, 2023

    Availability

    Academic Institutions and Nonprofits only

    

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15. pTLR repair vector

    Plasmid

    #64322

    Purpose

    Vector for repair of the traffic light reporter (TLR), providing the venus coding region

    Depositor

    Ralf Kuehn

    Article

    Chu et al Nat Biotechnol. 2015 Mar 24. doi: 10.103

    Insert

    Venus
    

    Use

    Artificial targeting vector

    Tags

    Expression

    Mutation

    see publication for details

    Promoter

    none

    Available since

    May 19, 2015

    Availability

    Academic Institutions and Nonprofits only

    

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16. pMHG-W57* reporter

    Plasmid

    #72850

    Purpose

    GFP-based reporter construct pMHG-W57* capable of detecting dose-dependent drug-induced PTC readthrough both by fluorescence microscopy and flow cytometry.

    Depositor

    Daniel Liebler

    Article

    Halvey et al FEBS Open Bio. 2012 Dec;2:56-59. Epub 20

    Insert

    GFP
    

    Use

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    Available since

    Jan. 26, 2016

    Availability

    Academic Institutions and Nonprofits only

    

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17. pmiR-report-hVEGFA

    Plasmid

    #112553

    Purpose

    3'UTR reporter assay

    Depositor

    Shusheng Wang

    Article

    Zhou et al Mol Ther. 2016 Oct;24(10):1823-1835. doi

    Insert

    VEGFA (VEGFA Human)
    

    Use

    Luciferase

    Tags

    Expression

    Mutation

    Promoter

    Available since

    July 6, 2018

    Availability

    Academic Institutions and Nonprofits only

    

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18. pcDNA5FRT/TO-SAKS1-Strep-HA

    Plasmid

    #113487

    Purpose

    Expression of human SAKS1 with C-terminal strep-HA tag

    Depositor

    Hemmo Meyer

    Article

    Hulsmann et al Mol Cell Proteomics. 2018 Mar 29. pii: R

    Insert

    SAKS1 (UBXN1 Human)
    

    Use

    Tags

    2xstrep-HA

    Expression

    Mammalian

    Mutation

    wildtype without stop codon

    Promoter

    CMV

    Available since

    Aug. 15, 2018

    Availability

    Academic Institutions and Nonprofits only

    

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19. SFTPB Promoter reporter

    Plasmid

    #88868

    Purpose

    Surfactant Protein B Gene Promoter Reporter

    Depositor

    David Mu

    Article

    Runkle et al J Biol Chem. 2012 Aug 17;287(34):28790-8

    Insert

    Surfactant protein B gene promoter (SFTPB Human)
    

    Use

    Luciferase

    Tags

    Expression

    Mutation

    Promoter

    Available since

    May 19, 2017

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

20. pAR127_StrepI_m(KS_MAT)_mouseL_ACP_H8_pET22b

    Plasmid

    #122850

    Purpose

    Expresses a designed construct of the condensing part fused to the ACP domain of murine type I fatty acid synthase (FASN) in Escherichia coli

    Depositor

    Martin Grininger

    Article

    Rittner et al Protein Sci. 2019 Feb;28(2):414-428. doi

    Insert

    m(KS_MAT)_mouseL_ACP (Fasn Mouse)
    

    Use

    Tags

    His8 tag and Twin-Strep tag

    Expression

    Bacterial

    Mutation

    wildtype

    Promoter

    T7 promoter

    Available since

    March 19, 2019

    Availability

    Academic Institutions and Nonprofits only

    

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