We narrowed to 10,855 results for: kars;
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Plasmid#116693PurposeLentiviral expression of TBC1D3B R162*DepositorInsertTBC1D3B (TBC1D3B Human)
UseLentiviralTagsExpressionMutationR162*PromoterAvailable sinceMarch 14, 2019AvailabilityAcademic Institutions and Nonprofits only -
pHAGE-NOTCH2NL-G137L
Plasmid#116439PurposeLentiviral expression of NOTCH2NL G137LDepositorInsertNOTCH2NL (NOTCH2NLA Human)
UseLentiviralTagsExpressionMutationG137LPromoterAvailable sinceMarch 14, 2019AvailabilityAcademic Institutions and Nonprofits only -
pHAGE-NOTCH2NL-Q172_G173delinsHC
Plasmid#116440PurposeLentiviral expression of NOTCH2NL Q172_G173delinsHCDepositorInsertNOTCH2NL (NOTCH2NLA Human)
UseLentiviralTagsExpressionMutationQ172_G173delinsHCPromoterAvailable sinceMarch 14, 2019AvailabilityAcademic Institutions and Nonprofits only -
pHAGE-NOTCH2NL-R87M
Plasmid#116441PurposeLentiviral expression of NOTCH2NL R87MDepositorInsertNOTCH2NL (NOTCH2NLA Human)
UseLentiviralTagsExpressionMutationR87MPromoterAvailable sinceMarch 14, 2019AvailabilityAcademic Institutions and Nonprofits only -
pHAGE-NOTCH2NL-S165F
Plasmid#116442PurposeLentiviral expression of NOTCH2NL S165FDepositorInsertNOTCH2NL (NOTCH2NLA Human)
UseLentiviralTagsExpressionMutationS165FPromoterAvailable sinceMarch 14, 2019AvailabilityAcademic Institutions and Nonprofits only -
pHAGE-OXA1L-L57F
Plasmid#116445PurposeLentiviral expression of OXA1L L57FDepositorInsertOXA1L (OXA1L Human)
UseLentiviralTagsExpressionMutationL57FPromoterAvailable sinceMarch 14, 2019AvailabilityAcademic Institutions and Nonprofits only -
pHAGE-OXA1L-P58S
Plasmid#116446PurposeLentiviral expression of OXA1L P58SDepositorInsertOXA1L (OXA1L Human)
UseLentiviralTagsExpressionMutationP58SPromoterAvailable sinceMarch 14, 2019AvailabilityAcademic Institutions and Nonprofits only -
EF1a_MYC Glu39Ala_P2A_Hygro_Barcode
Plasmid#120512PurposeBarcoded lentiviral vector to express MYC Glu39Ala in mammalian cells under control of EF1a promoter. Barcoded for transcript detection in single cell RNA-seq.DepositorInsertMYC Glu39Ala (MYC Human)
UseLentiviralTagsExpressionMutationPoint mutation changing Glutamic Acid to Alanine …PromoterEF1aAvailable sinceFeb. 8, 2019AvailabilityAcademic Institutions and Nonprofits only -
pEHISSSO6206
Plasmid#97065PurposeExpressing Sulfolobus solfataricus orf SSO6206 proteinDepositorInsertS. solfataricus orf SSO6206
UseTagsN-terminal TEV protease cleavable 6xHis tagExpressionMutationnonePromoterT7Available sinceAug. 2, 2017AvailabilityAcademic Institutions and Nonprofits only -
pQCXIX_sh_hBcl10_#16
Plasmid#75253Purposeretroviral shRNA vector against human BCL10, expresses GFP for transduction controlDepositorInsertshRNA against hBcl10 (BCL10 Human)
UseRNAi and RetroviralTagsExpressionMammalianMutationPromotermU6Available sinceJune 28, 2016AvailabilityAcademic Institutions and Nonprofits only -
pQCXIX_sh_hBcl10_#2
Plasmid#75254Purposeretroviral shRNA vector against human BCL10, expresses GFP for transduction controlDepositorInsertshRNA against hBcl10 (BCL10 Human)
UseRNAi and RetroviralTagsExpressionMammalianMutationPromotermU6Available sinceJune 28, 2016AvailabilityAcademic Institutions and Nonprofits only -
pQCXIX_sh_hBcl10_#1
Plasmid#75248Purposeretroviral shRNA vector against human BCL10, expresses GFP for transduction controlDepositorInsertshRNA against hBcl10 (BCL10 Human)
UseRNAi and RetroviralTagsExpressionMammalianMutationPromotermU6Available sinceJune 28, 2016AvailabilityAcademic Institutions and Nonprofits only -
pTH731-2µ-RLuc/minCFLuc
Plasmid#40601DepositorInsertsFirefly Luciferase
Renilla luciferase
UseTagsExpressionYeastMutationAll codons of the original FLuc gene were exchang…PromoterADH1 and TDH3Available sinceOct. 30, 2012AvailabilityAcademic Institutions and Nonprofits only -
pCMV-PEmax-P2A-GFP
Plasmid#180020PurposeMammalian expression of SpCas9 PEmax prime editor with P2A-EGFP markerDepositorInsertPEmax-P2A-EGFP
UseTagsP2A-EGFP, SV40 bpNLS, and c-Myc NLSExpressionMammalianMutationDetailed in manuscriptPromoterCMVAvailable sinceJan. 25, 2022AvailabilityAcademic Institutions and Nonprofits only -
pCMV-PE7-P2A-BSD
Plasmid#224122PurposeMammalian expression of SpCas9 PE7 prime editor with P2A-BSD markerDepositorInsertPE7-P2A-BSD
UseTagsP2A-BSD, SV40 bpNLS, and c-Myc NLSExpressionMammalianMutationPromoterCMVAvailable sinceAug. 19, 2024AvailabilityAcademic Institutions and Nonprofits only -
pDule2-3-nitroTyrosine (A7)
Plasmid#174079PurposePlasmid for incorporating the non-canonical amino acid 3-nitroTyrosine with the third generation Mj 3NY (A7) synthetase and cognate amber suppressing tRNA in Ecoli.DepositorInsert3-nitroTyrosine tRNA synthetase and cognate amber suppressing tRNA derived from M. jannaschii Tyrosine synthetase/tRNA system
UseTagsExpressionBacterialMutationY32H H70T D158H I159A L162RPromoterGlnRS (constitutive)Available sinceAug. 25, 2021AvailabilityAcademic Institutions and Nonprofits only -
Pan-QKI scFv [N147/6]
Plasmid#211605PurposeMammalian Expression Plasmid of Pan-QKI scFv. Derived from hybridoma N147/6.DepositorInsertPan-QKI (Homo sapiens) recombinant mouse scFv. (QKI Mouse)
UseTagsExpressionMammalianMutationPromoterCMVAvailable sinceJan. 16, 2024AvailabilityAcademic Institutions and Nonprofits only -
Aldh1L1 scFv [N103/31]
Plasmid#190522PurposeMammalian Expression of Aldh1L1 scFV. Derived from hybridoma N103/31.DepositorInsertAldh1L1 (Rattus norvegicus) recombinant scFV (Aldh1l1 Mouse)
UseTagsHA, Sortase, 6xHisExpressionMammalianMutationPromoterCMVAvailable sinceNov. 1, 2022AvailabilityAcademic Institutions and Nonprofits only -
Ma-Barnase
Plasmid#197572PurposeNegative selection plasmid for selecting ncAA-encoding Methanomethylophilus alvus Pyl-RS mutants. Expresses 2xTAG-codon interrupted barnase gene and M. alvus Pyl-tRNA(6). p15a origin of replication.DepositorInsertsBarnase - 2xTAG
M. alvus Pyl-tRNA (6)
UseTagsnoneExpressionBacterialMutation3TAG and 45TAGPromoteraraC and lppAvailable sinceMarch 21, 2023AvailabilityAcademic Institutions and Nonprofits only -
Ma-pREP
Plasmid#197571PurposePositive selection plasmid for selecting ncAA-encoding Methanomethylophilus alvus Pyl-RS mutants. Expresses TAG-codon interrupted chloramphenicol resistance cassette and M. alvus Pyl-tRNA(6). p15a oriDepositorInsertsChloramphenicol resistance gene - 111TAG
T7 polymerase -TAG
GFPuv
M. alvus Pyl-tRNA(6)
UseTagsExpressionBacterialMutation111TAG and 8TAG/112TAGPromoterAraC, T7, cat promoter (constitutive), and lpp (c…Available sinceApril 10, 2023AvailabilityAcademic Institutions and Nonprofits only -
PB-GFP-Gal8
Plasmid#127191PurposePiggybac transposon plasmid with CAG promoter GFP-Galectin 8 (GFP-Gal8) fusion protein. Useful as genetically encoded endosomal escape sensor.DepositorInsertGFP-Gal8 (LGALS8 Human)
UseTagsGal8 is fused to the c-terminus of GFPExpressionMammalianMutationPromoterCMVAvailable sinceDec. 9, 2019AvailabilityAcademic Institutions and Nonprofits only -
pCMV-PE7-P2A-GFP
Plasmid#222996PurposeMammalian expression of SpCas9 PE7 prime editor with P2A-EGFP markerDepositorInsertPE7-P2A-EGFP
UseTagsP2A-EGFP, SV40 bpNLS, and c-Myc NLSExpressionMammalianMutationPromoterCMVAvailable sinceAug. 16, 2024AvailabilityAcademic Institutions and Nonprofits only -
TDP-REGv2(Gaussia Luciferase)#5 in pTwist-CMV
Plasmid#216164PurposeExpression of secreted Gaussia Luciferase specifically in cells with TDP-43 loss of function. Note: It is not recommended to produce lentiviruses containing TDP-REG sequences – see Depositor CommentsDepositorInsertGaussia princeps luciferase (with double mutation to improve stability)
UseTagsExpressionMammalianMutationPromoterAvailable sinceJuly 1, 2024AvailabilityAcademic Institutions and Nonprofits only -
Str-KDEL_CD9-SBP-mCherry
Plasmid#222329PurposeSynchronize the trafficking of CD9 from the ER.DepositorInsertStreptavidin-KDEL and CD9 fused to SBP-mCherry (CD9 Human)
UseTagsExpressionMammalianMutationPromoterCMVAvailable sinceFeb. 4, 2025AvailabilityAcademic Institutions and Nonprofits only -
24xMoonTag-kif18b-STOP-24xSunTag-linker-24xPP7 (3’UTR translation reporter)
Plasmid#128605PurposeExpresses 24xgp41 peptide array (MoonTag peptide array), followed by kif18b gene, STOP codon, 24xgcn4 peptide array (SunTag peptide array), linker and 24 repeats of PP7 hairpins.DepositorInsertsUseTagsExpressionMammalianMutationPromoterPcmvAvailable sinceJan. 21, 2020AvailabilityAcademic Institutions and Nonprofits only -
pSecTaq2-hProS-Myc-6xHis
Plasmid#226524PurposeExpresses TAM Receptor Ligand human PROS1DepositorInsertHuman PROS1 (PROS1 Human)
UseTagsExpressionMammalianMutationPromoterCMVAvailable sinceNov. 26, 2024AvailabilityAcademic Institutions and Nonprofits only -
pFUSE2ss-CLIg-mK_M18
Plasmid#82358PurposeExpression plasmid coding for kappa light chain of mouse antibody specific to antigen B of the ABO blood group system, clone M18.DepositorInsertUseTagsnoneExpressionMammalianMutationPromoterhEF1-HTLV promAvailable sinceSept. 19, 2016AvailabilityAcademic Institutions and Nonprofits only -
Str-KDEL_CD9-SBP-EGFP
Plasmid#222328PurposeSynchronize the trafficking of CD9 from the ER.DepositorInsertStreptavidin-KDEL and CD9 fused to SBP-EGFP (CD9 Human)
UseTagsExpressionMammalianMutationPromoterCMVAvailable sinceFeb. 4, 2025AvailabilityAcademic Institutions and Nonprofits only -
pAAV-flex-taCasp3-TEVp
Plasmid#45580DepositorUseAAV; Adeno associated viral vectorTagsExpressionMutationLinker replaced with a TEV protease cleavage sitePromoterEF1aAvailable sinceJuly 10, 2013AvailabilityAcademic Institutions and Nonprofits only -
PE7_AAVS1_knockin_HDR_donor
Plasmid#223001PurposeHDR donor plasmid for PE7 knockin at AAVS1 locus. Left_homology_arm-splicing_acceptor-T2A-NeoR-bGHpA-pEF1α-PE7-IRES2-EGFP-WPRE-βglobinpA-right_homology_armDepositorInsertPE7
UseHdr donorTagsSV40 bpNLS and c-Myc NLSExpressionMammalianMutationPromoterEF1αAvailable sinceAug. 19, 2024AvailabilityAcademic Institutions and Nonprofits only