We narrowed to 35,837 results for: CaS

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Intron-Tagging-pX330-Cas9-mCherry

Plasmid

#159742

Purpose

Expresses Cas9-2A-mCherry and a sgRNA excising EGFP flanked by a splice acceptor and a splice donor from the Intron-Tagging-EGFP-Donor plasmid.

Depositor

Stefan Kubicek

Article

Reicher et al Genome Res. 2020 Dec;30(12):1846-1855. d

Insert

donor plasmid targeting sgRNA

Use

CRISPR

Expression

Mammalian

Promoter

U6

Available Since

Nov. 18, 2020

Availability

Academic Institutions and Nonprofits only

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pShHELIX(Cas12k-TniQ)-sgRNA_entry (CJT111)

Plasmid

#181790

Purpose

Expresses 3-component ShHELIX containing a Cas12k-TniQ fusion. New sgRNA spacer sequences can be added using Golden Gate assembly (via SapI sites).

Depositor

Benjamin Kleinstiver

Article

Tou et al Nat Biotechnol. 2023 Jan 2. doi: 10.1038

Insert

nAniI-ShTnsB, ShTnsC, ShCas12k-ShTniQ

Expression

Bacterial

Mutation

nAniI = K227M, F80K, L232K

Promoter

Lac and J23119

Available Since

Feb. 18, 2022

Availability

Academic Institutions and Nonprofits only

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pCAG_NLS-Cas9-XTEN-Ec73RT-NLS_pA_pCMV_BFP_pA

Plasmid

#176458

Purpose

Vector for encoding a human codon-optimized SpCas9-XTEN-Ec73RT fusion driven by CAG promoter and tagBFP driven by CMV promoter

Depositor

Hui Yang

Article

Kong et al Protein Cell. 2021 Aug 17. pii: 10.1007/

Insert

humanized S. pyogenes Cas9-XTEN-Ec73RT fusion

Use

CRISPR

Expression

Mammalian

Promoter

CAG

Available Since

Oct. 26, 2021

Availability

Academic Institutions and Nonprofits only

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MiniCoopR 2xU6:gRNA pten, mitfa:Cas9

Plasmid

#118845

Purpose

Targets ptena and ptenb and expresses zebrafish mitfa specifically in zebrafish melanocytes

Depositor

Leonard Zon

Article

Ablain et al Science. 2018 Nov 1. pii: science.aau650

Insert

ptena and ptenb (ptena, ptenb Zebrafish)

Use

CRISPR; Tol2

Available Since

March 21, 2019

Availability

Academic Institutions and Nonprofits only

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pAAV-pMecp2-dSaCas9-pU6-sgRNA

Plasmid

#159174

Purpose

Vector B encodes pAAV-pMecp2-dSaCas9-spA-pU6-sgRNA (BsaI) transgenes for AAV packaging and expression of dSaCas9-sgRNA in neurons

Depositor

Chung Tin

Article

Lau et al Mol Ther Nucleic Acids. 2019 Jun 7;16:63

Insert

dSaCas9

Use

AAV and CRISPR

Expression

Mammalian

Promoter

pMecp2

Available Since

Nov. 24, 2020

Availability

Academic Institutions and Nonprofits only

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pBIFC-flag-hCaspase 2 prodomain-VN173

Plasmid

#49262

Purpose

Expresses split fluorescence protein to measure caspase-2 dimerization in mammalian cells.

Depositor

Douglas Green

Article

Bouchier-Hayes et al Mol Cell. 2009 Sep 24;35(6):830-40. doi:

Insert

caspase-2 prodomain (CASP2 Human)

Tags

flag and split Venus N

Expression

Mammalian

Mutation

delete aa 148-435

Promoter

CMV

Available Since

Dec. 9, 2013

Availability

Academic Institutions and Nonprofits only
AAVS1 KanR foundation lox casette

Plasmid

#132388

Purpose

Foundation cassette containing lox sites for cre-mediated exchange of inducible vectors, confers mammalian G418 resistance, targets AAVS1 locus

Depositor

Gerold Schmitt-Ulms

Article

Wang et al Bio Protoc. 2020 May 5;10(9):e3615. doi:

Insert

G418 resistance

Use

CRISPR and Cre/Lox

Expression

Mammalian

Promoter

promoterless

Available Since

May 6, 2020

Availability

Academic Institutions and Nonprofits only

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pLV.U6.gDNMT1.Cas9-T2A-GFP

Plasmid

#170362

Purpose

Plasmid used for Crispr/cas9 based disruption of human DNMT1.

Depositor

Johan Jakobsson

Article

Jonsson et al Nat Commun. 2019 Jul 18;10(1):3182. doi:

Insert

Cas9-T2A-GFP

Use

Lentiviral

Available Since

June 27, 2021

Availability

Academic Institutions and Nonprofits only

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pspCas9-3'UTR-Tetra-com-vector

Plasmid

#132553

Purpose

Expresses spCas9 and sgRNA scaffold with com aptamer replacing the Tetraloop

Depositor

Baisong Lu

Article

Lu et al CRISPR J. 2021 Mar 16. doi: 10.1089/cris

Inserts

Cas9
sgRNA scaffold with com replacing the Tetraloop

Expression

Mammalian

Available Since

March 22, 2021

Availability

Academic Institutions and Nonprofits only

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pJEP305-pAAV-EFS-dSaCas9-KRAB-pA

Plasmid

#113681

Purpose

A EFS driven de-catalyzed SaCas9 fused to KRAB domain for inhibition of transcription in targeted region.

Depositor

Jonathan Ploski

Article

Kumar et al Front Mol Neurosci. 2018 Nov 13;11:413.

Insert

de-catalyzed SaCas9

Use

AAV and CRISPR

Tags

KRAB and NLS

Available Since

Jan. 22, 2019

Availability

Academic Institutions and Nonprofits only

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pCCL-PGK-SPdCas9-BFP-DNMT3A

Plasmid

#66819

Purpose

dCas9 fused to BFP and the human DNMT3A catalytic domain

Depositor

Yonglun Luo

Article

Lin et al Gigascience. 2018 Mar 1;7(3):1-19. doi:

Insert

dSpCas9-BFP-DNMT3A, catalytic domain of DNMT3A

Tags

dSpCas9-BFP-DNMT3A

Expression

Mammalian

Available Since

June 20, 2018

Availability

Academic Institutions and Nonprofits only

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pAAV-TRE-DIO-proCaspase-3-TEVp

Plasmid

#166607

Purpose

Encodes Cre-dependent procaspase 3 and TEVp under control of the TRE

Depositor

Martin Myers

Article

Sabatini et al eLife. 2021 Mar 11;10. pii: 66835. doi:

Insert

procaspase3- TEVp

Use

AAV

Promoter

tetracycline response element

Available Since

June 2, 2021

Availability

Academic Institutions and Nonprofits only

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XClone-Cas9-Cronus-T2A-BFP

Plasmid

#141209

Purpose

Tunable and temporal expression control of Cas9

Depositor

Rory Johnson

Insert

Cas9

Expression

Mammalian

Available Since

July 2, 2020

Availability

Academic Institutions and Nonprofits only

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PX458 pSp Cas9 Rhino1 tagA

Plasmid

#211627

Purpose

Cas9 Rhino1 tagA

Depositor

Agnel Sfeir

Article

Brambati et al Science. 2023 Aug 11;381(6658):653-660.

Insert

RHNO tagA

Expression

Mammalian

Promoter

U6

Available Since

Jan. 22, 2024

Availability

Academic Institutions and Nonprofits only

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pZac2.1 CMV173-SaCas9-U6-SaDMDR7-U6-SaDMDL2

Plasmid

#78606

Purpose

pZac2.1 CMV173-SaCas9-U6-SaDMDR7-U6-SaDMDL2

Depositor

Amy Wagers

Article

Tabebordbar et al Science. 2016 Jan 22;351(6271):407-11. d

Insert

CMV173-SaCas9-U6-SaDMDR7-U6-SaDMDL2

Use

AAV and CRISPR

Tags

HA tag

Expression

Mammalian

Promoter

173CMV, U6

Available Since

Dec. 16, 2017

Availability

Academic Institutions and Nonprofits only
pLenti-EF1a-SPdCas9-DNMT3B-2A-Blast

Plasmid

#71217

Purpose

dCas9 fused to human DNMT3B catalytic domain

Depositor

Yonglun Luo

Article

Lin et al Gigascience. 2018 Mar 1;7(3):1-19. doi:

Insert

dSpCas9-DNMT3B catalytic domain

Use

CRISPR and Lentiviral

Expression

Mammalian

Available Since

June 20, 2018

Availability

Academic Institutions and Nonprofits only

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pcDNA3.1-dCas9-MQ1(S317A)-EGFP

Plasmid

#89636

Purpose

Targeted CpG methylation

Depositor

Margaret Goodell

Article

Lei et al Nat Commun. 2017 Jul 11;8:16026. doi: 10

Insert

site-specific DNA-methyltransferase SssI

Use

CRISPR

Tags

3*FLAG and 6*His

Expression

Mammalian

Mutation

S317A, AGC-->GCC

Promoter

CMV

Available Since

July 12, 2017

Availability

Academic Institutions and Nonprofits only

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pU6-TREX1 g2 Cas9-T2A-mCherry

Plasmid

#164251

Purpose

Transcription of TREX1 guide RNA and Cas9 expression for CRISPR/Cas9 in mammalian cells

Depositor

John Maciejowski

Article

Mohr et al Mol Cell. 2021 Jan 12. pii: S1097-2765(2

Insert

sgTREX1

Use

CRISPR

Expression

Mammalian

Promoter

U6

Available Since

March 31, 2021

Availability

Academic Institutions and Nonprofits only

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pBiFC-flag-hCaspase 2 FL (C303S)-VN173

Plasmid

#49258

Purpose

Expresses split fluorescence protein to measure caspase-2 dimerization in mammalian cells.

Depositor

Douglas Green

Article

Bouchier-Hayes et al Mol Cell. 2009 Sep 24;35(6):830-40. doi:

Insert

caspase-2 (CASP2 Human)

Tags

flag and split Venus N

Expression

Mammalian

Mutation

C303S

Promoter

CMV

Available Since

May 6, 2014

Availability

Academic Institutions and Nonprofits only
pDGB3 alpha2 P35S:dCas9:VPR:Tnos (GB1826)

Plasmid

#160623

Purpose

TU for the constitutive expression of dCas9 fused to VPR (VP64-p65-Rta) activation domains

Depositor

Diego Orzaez

Article

Selma et al Plant Biotechnol J. 2019 Sep;17(9):1703-

Insert

P35S:dCas9:VPR-Tnos

Expression

Plant

Mutation

BsaI and BsmBI sites removed

Promoter

P35S

Available Since

March 25, 2021

Availability

Academic Institutions and Nonprofits only

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