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We narrowed to 6,190 results for: ARL

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Showing: 5701 - 5730 of 6190 results

  7. NanoLuc-PM-Venus

    Plasmid
    #164786
    Purpose
    Nanoluciferase (NanoLuc) targeted to the extracellular surface of the cell membrane and intracellularly labeled with monomeric Venus fluorescent protein
    Depositor
    Laszlo Hunyady
    Article
    Toth et al J Biol Chem. 2021 Feb 2:100366. doi: 10.
    Insert
    Nanoluciferase fused with the transmembrane domain of the platelet-derived growth factor receptor beta and Venus (PDGFRB Human, Oplophorus gracilirostris)
    Tags
    NanoLuc and mVenus
    Expression
    Mammalian
    Promoter
    CMV
    Available Since
    May 19, 2021
    Availability
    Academic Institutions and Nonprofits only
    164786-plasmid-map-sequence-id-326679

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  9. pLV-dCas9-KRAB-PGK-HygR

    Plasmid
    #83890
    Purpose
    Lentiviral Sp dCas9-KRAB fusion with Hygromycin B resistance cassette.
    Depositor
    Charles Gersbach
    Article
    Klann et al Nat Biotechnol. 2017 Apr 3. doi: 10.1038
    Inserts
    humanized dead Cas9 KRAB
    aminoglycoside phosphotransferase from E. coli
    Use
    CRISPR and Lentiviral
    Tags
    Flag
    Mutation
    D10A and H840A
    Promoter
    Human Ubiquitin C Promoter and mouse phosphoglyce…
    Available Since
    April 6, 2017
    Availability
    Academic Institutions and Nonprofits only
    83890-plasmid-map-sequence-id-240902

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  10. ADRA2B-Tango

    Plasmid
    #66217
    Purpose
    Expression of G protein-coupled receptors for PRESTO-Tango: parallel receptorome expression and screening via transcriptional output, with transcriptional activation following arrestin translocation
    Depositor
    Bryan Roth
    Article
    Kroeze et al Nat Struct Mol Biol. 2015 May;22(5):362-
    Insert
    ADRA2B (ADRA2B Human)
    Tags
    FLAG
    Expression
    Mammalian
    Promoter
    CMV
    Available Since
    Sept. 15, 2015
    Availability
    Academic Institutions and Nonprofits only

  16. pJW2098

    Plasmid
    #163094
    Purpose
    CRISPR repair template: add insertion site specific homology arms by PCR before insertion
    Depositor
    Jordan Ward
    Article
    Ashley et al Genetics. 2021 Mar 31;217(3). pii: 61045
    Insert
    30aa linker::mScarlet (dpi)::AID*::3xFLAG::10aa linker
    Use
    CRISPR
    Expression
    Worm
    Mutation
    Silent mutations to remove piRNA sites
    Available Since
    Jan. 5, 2021
    Availability
    Academic Institutions and Nonprofits only
    163094-plasmid-map-sequence-id-320582

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  18. ADRA2C-Tango

    Plasmid
    #66218
    Purpose
    Expression of G protein-coupled receptors for PRESTO-Tango: parallel receptorome expression and screening via transcriptional output, with transcriptional activation following arrestin translocation
    Depositor
    Bryan Roth
    Article
    Kroeze et al Nat Struct Mol Biol. 2015 May;22(5):362-
    Insert
    ADRA2C (ADRA2C Human)
    Tags
    FLAG
    Expression
    Mammalian
    Promoter
    CMV
    Available Since
    Sept. 15, 2015
    Availability
    Academic Institutions and Nonprofits only

  23. pAAV-Ef1a-DIO C1V1 (t/t)-TS-mCherry

    Plasmid
    #35498
    Purpose
    AAV expression of Ef1a-driven, cre-dependent, chimeric opsin variant C1V1 (E122T/E162T) for fast and potent optical excitation at red-shifted wavelengths.
    Depositor
    Karl Deisseroth
    Article
    Yizhar et al Nature. 2011 Jul 27;477(7363):171-8. doi
    Insert
    ChR1-VChR1 Chimera
    Use
    AAV
    Tags
    mCherry
    Expression
    Mammalian
    Mutation
    E122T and E162T
    Promoter
    Ef1a
    Available Since
    April 18, 2012
    Availability
    Academic Institutions and Nonprofits only
    35498-plasmid-map-sequence-id-199355

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  26. pAAV-CaMKIIa-C1V1 (t/t)-TS-EYFP

    Plasmid
    #35499
    Purpose
    AAV expression of CaMKII-driven chimeric opsin variant C1V1 (E122T/E162T) for fast and potent optical excitation at red-shifted wavelengths.
    Depositor
    Karl Deisseroth
    Article
    Yizhar et al Nature. 2011 Jul 27;477(7363):171-8. doi
    Has Service
    AAV9
    Insert
    ChR1-VChR1 Chimera
    Use
    AAV
    Tags
    EYFP
    Expression
    Mammalian
    Mutation
    E122T and E162T
    Promoter
    CaMKIIa
    Available Since
    April 18, 2012
    Availability
    Academic Institutions and Nonprofits only
    35499-plasmid-map-sequence-id-199323

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  27. pAAV-Ef1a-DIO C1V1 (t/t)-TS-EYFP

    Plasmid
    #35497
    Purpose
    AAV expression of Ef1a-driven, cre-dependent, chimeric opsin variant C1V1 (E122T/E162T) for fast and potent optical excitation at red-shifted wavelengths.
    Depositor
    Karl Deisseroth
    Article
    Yizhar et al Nature. 2011 Jul 27;477(7363):171-8. doi
    Has Service
    AAV9
    Insert
    ChR1-VChR1 Chimera
    Use
    AAV
    Tags
    EYFP
    Expression
    Mammalian
    Mutation
    E122T and E162T
    Promoter
    Ef1a
    Available Since
    April 18, 2012
    Availability
    Academic Institutions and Nonprofits only
    35497-plasmid-map-sequence-id-196845

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Showing: 5701 - 5730 of 6190 results