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pMAL-c5X-BRT17

Plasmid

#67000

Purpose

Expresses malE-BRT17 in E. coli

Depositor

Ashutosh Chilkoti

Article

Tang et al Nat Mater. 2016 Jan 4. doi: 10.1038/nmat

Insert

malE-BRT17

Tags

His6 and malE-Factor Xa

Expression

Bacterial

Promoter

tac

Available Since

Jan. 5, 2016

Availability

Academic Institutions and Nonprofits only

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pKB255

Plasmid

#170038

Purpose

Encodes LacI-controlled expression of surface-displayed melittin and PvirK-mNeonGreen reporter

Depositor

Jeffrey Tabor

Article

Brink et al bioRxiv

Inserts

mNeonGreen
Melittin

Use

Synthetic Biology

Tags

Lpp, OmpA, and Tether

Expression

Bacterial

Promoter

Ptac and PvirK

Available Since

Jan. 19, 2022

Availability

Academic Institutions and Nonprofits only

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pMAL-c5X-Dros16

Plasmid

#67004

Purpose

Expresses malE-Dros16 in E. coli

Depositor

Ashutosh Chilkoti

Article

Tang et al Nat Mater. 2016 Jan 4. doi: 10.1038/nmat

Insert

malE-Dros16

Tags

His6 and malE-Factor Xa

Expression

Bacterial

Promoter

tac

Available Since

Jan. 5, 2016

Availability

Academic Institutions and Nonprofits only

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p142

Plasmid

#8385

Depositor

Jeffrey Green

Article

Kaczmarczyk et al Nucleic Acids Res 2001 Jun 15;29(12):E56

Insert

LOX I/LOX II

Use

Cre/Lox

Tags

EGFP

Expression

Mammalian

Available Since

April 28, 2006

Availability

Academic Institutions and Nonprofits only
pMAL-c5X-CLP3.7

Plasmid

#67011

Purpose

Expresses malE-CLP3.7 in E. coli

Depositor

Ashutosh Chilkoti

Article

Tang et al Nat Mater. 2016 Jan 4. doi: 10.1038/nmat

Insert

malE-CLP3.7

Tags

His6 and malE-Factor Xa

Expression

Bacterial

Promoter

tac

Available Since

Jan. 5, 2016

Availability

Academic Institutions and Nonprofits only

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p138

Plasmid

#8384

Depositor

Jeffrey Green

Article

Kaczmarczyk et al Nucleic Acids Res 2001 Jun 15;29(12):E56

Insert

LOX I

Use

Cre/Lox

Tags

EGFP

Expression

Mammalian

Available Since

April 28, 2006

Availability

Academic Institutions and Nonprofits only
pMAL-c5X-SPR16

Plasmid

#67001

Purpose

Expresses malE-SPR16 in E. coli

Depositor

Ashutosh Chilkoti

Article

Tang et al Nat Mater. 2016 Jan 4. doi: 10.1038/nmat

Insert

malE-SPR16

Tags

His6 and malE-Factor Xa

Expression

Bacterial

Promoter

tac

Available Since

Jan. 5, 2016

Availability

Academic Institutions and Nonprofits only

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pMAL-c5X-AB12

Plasmid

#67005

Purpose

Expresses malE-AB12 in E. coli

Depositor

Ashutosh Chilkoti

Article

Tang et al Nat Mater. 2016 Jan 4. doi: 10.1038/nmat

Insert

malE-AB12

Tags

His6 and malE-Factor Xa

Expression

Bacterial

Promoter

tac

Available Since

Jan. 5, 2016

Availability

Academic Institutions and Nonprofits only

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pMAL-c5X-BQ_6

Plasmid

#67006

Purpose

Expresses malE-BQ_6 in E. coli

Depositor

Ashutosh Chilkoti

Article

Tang et al Nat Mater. 2016 Jan 4. doi: 10.1038/nmat

Insert

malE-BQ_6

Tags

His6 and malE-Factor Xa

Expression

Bacterial

Promoter

tac

Available Since

Jan. 5, 2016

Availability

Academic Institutions and Nonprofits only

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XNOR-gate-V2.0-ASV

Plasmid

#44455

Depositor

Drew Endy

Article

Bonnet et al Science. 2013 May 3;340(6132):599-603. d

Insert

XNOR-gate

Use

Synthetic Biology

Expression

Bacterial

Available Since

May 13, 2013

Availability

Academic Institutions and Nonprofits only

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35S:RUBY

Plasmid

#160908

Purpose

RUBY under the control of CaMV 35S promoter/marker for transformation

Depositor

Yunde Zhao

Article

He et al Hortic Res. 2020 Sep 19;7:152. doi: 10.1

Insert

RUBY and CaMV 35S promoter

Expression

Plant

Promoter

CaMV 35S

Available Since

Nov. 5, 2020

Availability

Academic Institutions and Nonprofits only

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pCAG-Cre-IRES2-GFP

Plasmid

#26646

Purpose

Mammalian coexpression of nls-Cre and EGFP from the CAG promoter

Depositor

Anjen Chenn

Article

Woodhead et al J Neurosci. 2006 Nov 29;26(48):12620-30.

Insert

Cre

Use

Cre/Lox

Tags

GFP and IRES2

Expression

Mammalian

Promoter

CAG

Available Since

Dec. 2, 2010

Availability

Academic Institutions and Nonprofits only

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RpHLuorin2

Plasmid

#180227

Purpose

for bacterial expression of recombinant ratiometric pHluorin2

Depositor

Geert van den Bogaart

Article

Linders et al ACS Chem Biol. 2022 Jan 21;17(1):240-251

Insert

Ratiometric pHLuorin2

Tags

His-tag followed by thrombin cleavage site

Expression

Bacterial

Promoter

T7

Available Since

March 4, 2022

Availability

Academic Institutions and Nonprofits only

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pHAGE-EFS-MCP-HALOnls

Plasmid

#121937

Purpose

CRISPR-Sirius plasmid

Depositor

Thoru Pederson

Article

Ma et al Nat Methods. 2018 Nov;15(11):928-931. do

Insert

MCP-HALOnls

Use

Lentiviral

Tags

HaloTag

Expression

Mammalian

Promoter

EFS

Available Since

Feb. 8, 2019

Availability

Academic Institutions and Nonprofits only

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pHR-scFv-GCN4-sfGFP-GB1-dWPRE

Plasmid

#60907

Purpose

The plasmid encodes an antibody that binds to the GCN4 peptide from the SunTag system, and is fused to sfGFP for imaging

Depositor

Ron Vale

Article

Tanenbaum et al Cell. 2014 Oct 8. pii: S0092-8674(14)012

Insert

scFv-GCN4

Use

Lentiviral

Expression

Mammalian

Available Since

Jan. 15, 2015

Availability

Academic Institutions and Nonprofits only

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pCDNA3.1-YY1

Plasmid

#169018

Purpose

Expression of YY1 driven by CMV promoter

Depositor

Claes Wadelius

Article

Pan et al Life Sci Alliance. 2021 Jun 7;4(7). pii:

Insert

human YY1 (YY1 Human)

Expression

Mammalian

Promoter

CMV

Available Since

June 14, 2021

Availability

Academic Institutions and Nonprofits only

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pET28-MBP-TEV

Plasmid

#69929

Purpose

bacterial expression of MBP-TEV n-terminal fusions

Depositor

Zita Balklava, Thomas Wassmer

Article

Currinn et al Cell Mol Life Sci. 2015 Jul 28.

Has Service

Cloning Grade DNA

Type

Empty backbone

Tags

MBP and TEV cleavage site

Expression

Bacterial

Promoter

T7-lacO (lactose/IPTG inducible)

Available Since

Jan. 8, 2016

Availability

Academic Institutions and Nonprofits only

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pET263-pUC57 24xMS2V7

Plasmid

#140705

Purpose

MS2V7 cloning

Depositor

Robert Singer, Evelina Tutucci

Article

Tutucci et al Nat Methods. 2017 Nov 13. doi: 10.1038/n

Insert

24xMS2V7

Use

Cloning vector

Mutation

24 MS2 loops V7, high-affinity C-variant, inter-s…

Promoter

None

Available Since

April 29, 2020

Availability

Academic Institutions and Nonprofits only

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pHR-scFv-GCN4-sfGFP-GB1-NLS-dWPRE

Plasmid

#60906

Purpose

The plasmid encodes an antibody that binds to the GCN4 peptide from the SunTag system, and is fused to sfGFP and a NLS for imaging

Depositor

Ron Vale

Article

Tanenbaum et al Cell. 2014 Oct 8. pii: S0092-8674(14)012

Insert

scFv-GCN4

Use

Lentiviral

Expression

Mammalian

Available Since

Nov. 17, 2014

Availability

Academic Institutions and Nonprofits only

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pET246-pUC57 12xMS2V6

Plasmid

#104390

Purpose

MS2V6 cloning

Depositor

Robert Singer, Evelina Tutucci

Article

Tutucci et al Nat Methods. 2017 Nov 13. doi: 10.1038/n

Insert

12xMS2V6

Expression

Bacterial

Mutation

All Loops are U-variants, interspaced by 50 nucle…

Available Since

Dec. 21, 2017

Availability

Academic Institutions and Nonprofits only

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pcDNA5/FRT/TO_[Cox8a]x2_HaloTag9

Plasmid

#175539

Purpose

Expression of HaloTag9 at the inner membrane of mitochondria in mammalian cells

Depositor

Kai Johnsson

Article

Frei et al Nat Methods. 2021 Dec 16. pii: 10.1038/s

Insert

Cox8a-HaloTag9

Expression

Mammalian

Mutation

HaloTag9 = HaloTag7-Q165H-P174R

Available Since

Dec. 13, 2021

Availability

Academic Institutions and Nonprofits only

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pET28a-SUPREM

Plasmid

#205023

Purpose

Expressing SUPer Rna Ecogii Methyltransferase (SUPREM)

Depositor

Paola Laurino

Article

Ochiai et al Nucleic Acids Res. 2024 Oct 17:gkae887.

Insert

SUPer Rna Ecogii Methyltransferase

Tags

His6x tag

Expression

Bacterial

Promoter

T7

Available Since

Oct. 18, 2024

Availability

Academic Institutions and Nonprofits only

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pET28a-AncMEcoGII291

Plasmid

#205024

Purpose

Expressing engineered M.EcoGII with higher thermostability and DNA methylation activity

Depositor

Paola Laurino

Article

Ochiai et al Nucleic Acids Res. 2024 Oct 17:gkae887.

Insert

Ancestral M.EcoGII 291

Tags

His6x tag

Expression

Bacterial

Promoter

T7

Available Since

Oct. 18, 2024

Availability

Academic Institutions and Nonprofits only

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pCDH-TMEM106b-mApple

Plasmid

#179385

Purpose

Lentiviral vector for expression of TMEM106b fused mApple

Depositor

Angus Johnston

Article

Rennick et al Nat Commun. 2022 Oct 12;13(1):6023. doi:

Insert

TMEM106b - mApple

Use

Lentiviral

Tags

mApple

Expression

Mammalian

Promoter

EF1

Available Since

Oct. 11, 2022

Availability

Academic Institutions and Nonprofits only

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pCDNA3.1_3XFlag

Plasmid

#182494

Purpose

Empty plasmid containing Flag tag for C-terminal tagging

Depositor

Paolo Comoglio

Article

Cerqua et al Life Sci Alliance. 2022 May 30;5(10). pi

Insert

3xFlag with 5 Glycolic spacer

Available Since

June 16, 2022

Availability

Academic Institutions and Nonprofits only

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L4440_BioBrick-sgRNA

Plasmid

#177783

Purpose

Overexpression of sgRNAs in E. coli HT115, sgRNA overexpression scaffold; for ingestion by C. elegans

Depositor

Michael Ristow

Article

Fischer et al J Biol Chem. 2022 May 27:102085. doi: 10

Type

Empty backbone

Expression

Bacterial

Available Since

June 1, 2022

Availability

Academic Institutions and Nonprofits only

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TRMPVIR

Plasmid

#27994

Purpose

Retroviral transfer plasmid for Tet-regulated shRNA expression with a minimal CMV promoter. Contains DsRed2 and Venus fluorescent reporters.

Depositor

Scott Lowe

Article

Zuber et al Nat Biotechnol. 2011 Jan . 29(1):79-83.

Insert

TRMPVIR (shRen)

Use

RNAi and Retroviral; Fluorescent reporters

Expression

Mammalian

Available Since

March 18, 2011

Availability

Academic Institutions and Nonprofits only

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pLX-TRV1

Plasmid

#180515

Purpose

Agrobacterium tumefaciens-based expression of TRV1

Depositor

Jose-Antonio Daros

Article

Aragones et al Biotechnol J. 2022 Mar 24:e2100504. doi:

Insert

Tobacco rattle virus RNA1

Expression

Plant

Promoter

35S CaMV

Available Since

April 12, 2022

Availability

Academic Institutions and Nonprofits only

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pcDNA5/FRT/TO_Lyn11-HaloTag9

Plasmid

#175543

Purpose

Expression of HaloTag9 at the inner leaflet of the plasma membrane of mammalian cells

Depositor

Kai Johnsson

Article

Frei et al Nat Methods. 2021 Dec 16. pii: 10.1038/s

Insert

Lyn11-HaloTag9

Expression

Mammalian

Mutation

HaloTag9 = HaloTag7-Q165H-P174R

Available Since

Dec. 13, 2021

Availability

Academic Institutions and Nonprofits only

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pcDNA3.1(-)-qmTQ2-cAMP

Plasmid

#225250

Purpose

mammalian expression of qmTQ2-cAMP, a cyan fluorescent FLIM biosensor for detecting cAMP

Depositor

Yasushi Okada

Article

Zhong et al Cell Rep Methods. 2024 Nov 18;4(11):1009

Insert

qmTQ2-cAMP

Expression

Mammalian

Promoter

CMV promoter

Available Since

Sept. 20, 2024

Availability

Academic Institutions and Nonprofits only

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pSico-shNKCC1

Plasmid

#83027

Purpose

expresses an shRNA against NKCC1

Depositor

Angelique Bordey

Article

Young et al J Neurosci. 2012 Sep 26;32(39):13630-8.

Insert

shNKCC1

Use

Cre/Lox, Lentiviral, and RNAi

Expression

Mammalian

Promoter

U6

Available Since

Oct. 25, 2016

Availability

Academic Institutions and Nonprofits only

Enlarge
pcDNA5/FRT/TO_[Cox8a]x2_HaloTag7

Plasmid

#175529

Purpose

Expression of HaloTag7 at the inner membrane of mitochondria in mammalian cells

Depositor

Kai Johnsson

Article

Frei et al Nat Methods. 2021 Dec 16. pii: 10.1038/s

Insert

Cox8a-HaloTag7

Expression

Mammalian

Available Since

Dec. 13, 2021

Availability

Academic Institutions and Nonprofits only

Enlarge
pET259-pUC57 24xMS2V6

Plasmid

#104391

Purpose

MS2V6 cloning

Depositor

Robert Singer, Evelina Tutucci

Article

Tutucci et al Nat Methods. 2017 Nov 13. doi: 10.1038/n

Insert

24xMS2V6

Expression

Bacterial

Mutation

All Loops are U-variants, interspaced by 50 nucle…

Available Since

Jan. 2, 2018

Availability

Academic Institutions and Nonprofits only

Enlarge
pLV-hCDC6p-Venus-puro

Plasmid

#212666

Purpose

Express Venus under CDC6 promoter

Depositor

Yumi Konagaya, Tobias Meyer

Article

Konagaya et al Nature. 2024 Jul;631(8020):424-431. doi:

Insert

hCDC6p-Venus

Use

Lentiviral

Promoter

hCDC6p

Available Since

Aug. 5, 2024

Availability

Academic Institutions and Nonprofits only

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pBAD/HisB (TIREVOL)-mEos3.2

Plasmid

#189720

Purpose

titratable expression of mEos3.2 in bacteria

Depositor

Daniel Daley

Article

Shilling et al Synth Biol (Oxf). 2022 Jul 5;7(1):ysac00

Insert

mEos3.2

Tags

His-T7-EK

Expression

Bacterial

Promoter

pBAD

Available Since

Oct. 4, 2022

Availability

Academic Institutions and Nonprofits only

Enlarge
AeU6 3p3 GFP LgRNA

Plasmid

#182208

Purpose

For generation of plasmids for CRISPR-mediated 3p3 GFP knockin in Aedes aegypti

Depositor

Craig Montell

Article

Chen et al Genetics. 2021 Nov 5;219(3). pii: 636806

Insert

3xP3-GFP

Use

CRISPR

Expression

Insect

Promoter

U6

Available Since

April 7, 2022

Availability

Academic Institutions and Nonprofits only

Enlarge
pCAG-GFP1 POD-nAb-WPRE

Plasmid

#244973

Purpose

Expresses a fusion protein of a nanobody against GFP and peroxidase (POD) in mammalian cells

Depositor

Hiroyuki Hioki

Article

Yamauchi et al Commun Biol. 2025 Jun 18;8(1):903. doi:

Insert

GFP1 POD-nAb

Available Since

Oct. 20, 2025

Availability

Academic Institutions and Nonprofits only

Enlarge
pCAG-RFP6 POD-nAb-WPRE

Plasmid

#244974

Purpose

Expresses a fusion protein of a nanobody against RFP and peroxidase (POD) in mammalian cells

Depositor

Hiroyuki Hioki

Article

Yamauchi et al Commun Biol. 2025 Jun 18;8(1):903. doi:

Insert

RFP6 POD-nAb

Available Since

Oct. 20, 2025

Availability

Academic Institutions and Nonprofits only

Enlarge
pcDNA5/FRT/TO_CalR_HaloTag7_KDEL

Plasmid

#175530

Purpose

Expression of HaloTag7 in the endoplasmic reticulum of mammalian cells

Depositor

Kai Johnsson

Article

Frei et al Nat Methods. 2021 Dec 16. pii: 10.1038/s

Insert

CalR-HaloTag7-KDEL

Expression

Mammalian

Available Since

Dec. 13, 2021

Availability

Academic Institutions and Nonprofits only

Enlarge
pcDNA5/FRT/TO_CalR_HaloTag9_KDEL

Plasmid

#175540

Purpose

Expression of HaloTag9 in the endoplasmic reticulum of mammalian cells

Depositor

Kai Johnsson

Article

Frei et al Nat Methods. 2021 Dec 16. pii: 10.1038/s

Insert

CalR-HaloTag9-KDEL

Expression

Mammalian

Mutation

HaloTag9 = HaloTag7-Q165H-P174R

Available Since

Dec. 13, 2021

Availability

Academic Institutions and Nonprofits only

Enlarge

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