We narrowed to 28,682 results for: Met;
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Plasmid#231927PurposeCre-off/Flp-on EYFP under the Ef1a promoterDepositorInserteYFP with introns
UseAAVTagsExpressionMutationPromoterEf1aAvailable sinceJan. 22, 2025AvailabilityAcademic Institutions and Nonprofits only -
pSCrhaB2_ApyOAHID
Plasmid#226245PurposeProduction of ApyA peptide modified by the B12-rSAM enzyme ApyD (optional, refer to publication), the cytochrome P450 enzyme ApyO, and the MNIO enzyme ApyHIDepositorInsertsHis6_ApyA
ApyD
ApyO
ApyH
ApyI
UseTagsHexa-HistidineExpressionBacterialMutationPromoterAvailable sinceOct. 23, 2024AvailabilityAcademic Institutions and Nonprofits only -
pLV-EF1a-NLS-msfGFP-T2A-mCherry-NES> mPGK-PuroR
Plasmid#218916PurposeLentiviral construct expressing msfGFP and nuclear mCherry proteins.DepositorInsertEF1a-msfGFP-T2A-mCherry-NLS > mPGK-PuroR
UseLentiviralTagsExpressionMutationPromoterAvailable sinceJune 4, 2024AvailabilityAcademic Institutions and Nonprofits only -
CCM2_I432D_eGFP
Plasmid#208879PurposeExpress eGFP-CCM2 with the I432D mutation in mammalian cells.DepositorInsertCCM2 (CCM2 Human)
UseTagseGFPExpressionMammalianMutationmutation I432DPromoterAvailable sinceDec. 22, 2023AvailabilityAcademic Institutions and Nonprofits only -
CCM2_W421AD422A_eGFP
Plasmid#208880PurposeExpress eGFP-CCM2 with the W421A and D422A mutations in mammalian cells.DepositorInsertCCM2 (CCM2 Human)
UseTagseGFPExpressionMammalianMutationmutations W421A and D422APromoterAvailable sinceDec. 22, 2023AvailabilityAcademic Institutions and Nonprofits only -
CCM2_I428S_eGFP
Plasmid#208878PurposeExpress eGFP-CCM2 with the I428S mutation in mammalian cells.DepositorInsertCCM2 (CCM2 Human)
UseTagseGFPExpressionMammalianMutationmutation I428SPromoterAvailable sinceDec. 22, 2023AvailabilityAcademic Institutions and Nonprofits only -
pAS_4xKBstTyrT(CUA)_EF1_sfGFP 102TAG 150TAA
Plasmid#174895Purposeamber and ochre dual suppression reporter sfGFP 102TAG 150 TAA expression, with BstTyr(CUA) amber suppressor tRNA cassetteDepositorInsertsfGFP
UseTagsExpressionMammalianMutation102TAG 150TAA in sfGFPPromoterEF1Available sinceNov. 7, 2023AvailabilityAcademic Institutions and Nonprofits only -
pDEST40-CES2C-ΔC
Plasmid#200555PurposeMammalian expression of secreted mouse CES2CDepositorInsertMouse Carboxylesterase 2C delta C-terminal (Ces2c Mouse)
UseTags6xHis, Flag, and V5ExpressionMammalianMutationPromoterCMV promoterAvailable sinceMay 8, 2023AvailabilityAcademic Institutions and Nonprofits only -
pAAV-CAGGS-Flex/3'USS(103)-GFP
Plasmid#197890PurposeCan be used to generate AAV virus that will express GFP in the presence of Cre including the shortage of a unilateral spacer sequence(USS)DepositorInsertGFP
UseAAVTagsExpressionMutationN/APromoterCAGAvailable sinceMarch 15, 2023AvailabilityAcademic Institutions and Nonprofits only -
pAAV-CAGGS-Flex/3'USS(553)-GFP
Plasmid#197888PurposeCan be used to generate AAV virus that will express GFP in the presence of Cre including the shortage of a unilateral spacer sequence(USS)DepositorInsertGFP
UseAAVTagsExpressionMutationN/APromoterCAGAvailable sinceMarch 15, 2023AvailabilityAcademic Institutions and Nonprofits only -
pAAV-CAGGS-Flex/3'USS(285)-GFP
Plasmid#197889PurposeCan be used to generate AAV virus that will express GFP in the presence of Cre including the shortage of a unilateral spacer sequence(USS)DepositorInsertGFP
UseAAVTagsExpressionMutationN/APromoterCAGAvailable sinceMarch 15, 2023AvailabilityAcademic Institutions and Nonprofits only -
pRSFDuet-1_mNeongreen-Erythropoietin
Plasmid#182422PurposeBacterial expression of mNeongreen-Erythropoietin fusionDepositorInsertmNeongreen-eythropoietin (EPO Human)
UseTagsmNeongreenExpressionBacterialMutationPromoterT7Available sinceFeb. 3, 2023AvailabilityAcademic Institutions and Nonprofits only -
deltaVP1d63C + VP2C-GFPdeg
Plasmid#182481PurposeYIp for expressing Murine polyomavirus deltaVP1 with the 63 C-ter residues truncated (GAL1 promoter) and destabilised GFP tagged with VP2C (GAL10 promoter). Contains uracil marker (K. lactis URA3).DepositorInsertsVP2C-GFPdeg
deltaVP1d63C
UseSynthetic Biology; Metabolic engineeringTagsExpressionYeastMutationPromoterGAL1 and GAL10Available sinceDec. 6, 2022AvailabilityAcademic Institutions and Nonprofits only -
Linked VLP NES
Plasmid#182432PurposeYeast integrative plasmid for expressing Murine polyomavirus deltaVP1 (GAL1 promoter) and fusion protein VP2C-ERG20-AcNES1 (GAL10 promoter). Contains uracil auxotrophic marker (K. lactis URA3).DepositorInsertsVP2C-FPPS-NES
deltaVP1
UseSynthetic Biology; Metabolic engineeringTagsExpressionYeastMutationPromoterGAL1 and GAL10Available sinceDec. 6, 2022AvailabilityAcademic Institutions and Nonprofits only -
Linked VLP NES (M)
Plasmid#182434PurposeYIp for expressing Murine polyomavirus deltaVP1 (GAL1 promoter) and fusion protein VP2C-ERG20(F96W-N127W)-AcNES1 (GAL10 promoter). Contains uracil marker (K. lactis URA3).DepositorInsertsVP2C-FPPS(M)-NES
deltaVP1
UseSynthetic Biology; Metabolic engineeringTagsExpressionYeastMutationPromoterGAL1 and GAL10Available sinceDec. 6, 2022AvailabilityAcademic Institutions and Nonprofits only -
Linked VLP NES (G)
Plasmid#182476PurposeYIp for expressing Murine polyomavirus deltaVP1 (GAL1 promoter) and fusion protein VP2C-ERG20(F96C)-AcNES1 (GAL10 promoter). Contains uracil marker (K. lactis URA3).DepositorInsertsVP2C-FPPS(G)-NES
deltaVP1
UseSynthetic Biology; Metabolic engineeringTagsExpressionYeastMutationPromoterGAL1 and GAL10Available sinceDec. 6, 2022AvailabilityAcademic Institutions and Nonprofits only -
pNHT7::UMSBP::sNluc2_ORF2
Plasmid#186766PurposeAn mRNA production vector encoding planarian codon-optimized sNluc2 with UMSBP 5' and 3' UTRs beginning at a start codon further downstream of the endogenous gene's start codon..DepositorInsertNanoluciferase
UseTagsExpressionMutationPromoterT7Available sinceOct. 24, 2022AvailabilityAcademic Institutions and Nonprofits only -
pCEP4_HLA-cmyc-GRM3 C127A T174A-VC
Plasmid#140457PurposeMammalian expression plasmid for c-myc-tagged mGluR3 C127A and T174A mutant fused to the C-terminal half of split fluorescent Venus (VC)DepositorInsertGRM3 (GRM3 Human)
UseTagsSignal/leader sequence from HLA class I histocomp…ExpressionMammalianMutationCysteine 127 to alanine, Threonine 174 to alaninePromoterAvailable sinceJuly 11, 2022AvailabilityAcademic Institutions and Nonprofits only -
pPodA11
Plasmid#162843PurposeExpression protein designed PodA10 from a TEV cleavable IPTG inducible plasmid with ampicillin resistanceDepositorInsertProtein Designed Pyocyanin demethylase
UseTagsTEV-cleavable 6x-His tagExpressionBacterialMutationA53N, I73T, A87V, M99V, A129T from WT PodAPromoterT7Available sinceSept. 30, 2021AvailabilityAcademic Institutions and Nonprofits only -
pCSDest2-2XNLS-SpCas9-MT3-NLS-3XHA-NLS-TALentry
Plasmid#69233PurposeR1335K mutant SpCas9 expression plasmid to clone TALEs through BbsI site (generates compatible overhangs with Acc65I and BamHI sites)DepositorInsertSpCas9
UseCRISPRTags2X NLS, BbsI TALE entry cassette, and NLS-3XHA-NL…ExpressionMammalianMutationArginine 1335 is changed to LysinePromoterAvailable sinceNov. 17, 2015AvailabilityAcademic Institutions and Nonprofits only -
pLKO1-puro-U6-sgRNA-KANK3
Plasmid#69238PurposeU6 driven SpCas9 sgRNA expression for KANK3 siteDepositorInsertKANK3 sgRNA (KANK3 Human)
UseCRISPR and LentiviralTagsExpressionMammalianMutationPromoterU6Available sinceOct. 20, 2015AvailabilityAcademic Institutions and Nonprofits only -
SL542
Plasmid#49951PurposeExpresses a TALE-TET1CD (catalytic domain of TET1) fusion protein engineered to bind a site in the human RHOXF2/2B homeobox (RHOXF2) gene and demethylate CpGs adjacent to the binding siteDepositorInsertTet1CD RH-8 (RHOXF2 Human)
UseTALENTagsExpressionMutationPromoterEF1aAvailable sinceJan. 22, 2014AvailabilityAcademic Institutions and Nonprofits only -
SL544
Plasmid#49970PurposeExpresses a TALE-TET1CD (catalytic domain of TET1) fusion protein engineered to bind a site in the human RHOXF2/2B homeobox (RHOXF2) gene and demethylate CpGs adjacent to the binding siteDepositorInsertTet1CD RH-10 (RHOXF2 Human)
UseTALENTagsExpressionMutationPromoterEF1aAvailable sinceDec. 10, 2013AvailabilityAcademic Institutions and Nonprofits only -
SL545
Plasmid#49953PurposeExpresses a TALE-TET1CD (catalytic domain of TET1) fusion protein engineered to bind a site in the human RHOXF2/2B homeobox (RHOXF2) gene and demethylate CpGs adjacent to the binding siteDepositorInsertTet1CD RH-11 (RHOXF2 Human)
UseTALENTagsExpressionMutationPromoterEF1aAvailable sinceDec. 10, 2013AvailabilityAcademic Institutions and Nonprofits only -
SL543
Plasmid#49952PurposeExpresses a TALE-TET1CD (catalytic domain of TET1) fusion protein engineered to bind a site in the human RHOXF2/2B homeobox (RHOXF2) gene and demethylate CpGs adjacent to the binding siteDepositorInsertTet1CD RH-9 (RHOXF2 Human)
UseTALENTagsExpressionMutationPromoterEF1aAvailable sinceDec. 10, 2013AvailabilityAcademic Institutions and Nonprofits only -
SL541
Plasmid#49950PurposeExpresses a TALE-TET1CD (catalytic domain of TET1) fusion protein engineered to bind a site in the human RHOXF2/2B homeobox (RHOXF2) gene and demethylate CpGs adjacent to the binding siteDepositorInsertTet1CD RH-7 (RHOXF2 Human)
UseTALENTagsExpressionMutationPromoterEF1aAvailable sinceDec. 10, 2013AvailabilityAcademic Institutions and Nonprofits only -
SL540
Plasmid#49949PurposeExpresses a TALE-TET1CD (catalytic domain of TET1) fusion protein engineered to bind a site in the human RHOXF2/2B homeobox (RHOXF2) gene and demethylate CpGs adjacent to the binding siteDepositorInsertTet1CD RH-6 (RHOXF2 Human)
UseTALENTagsExpressionMutationPromoterEF1aAvailable sinceDec. 10, 2013AvailabilityAcademic Institutions and Nonprofits only -
AB.pCCL.sin.cPPT.U6.miR-874-Decoy.hPGK.GFP.WPRE
Plasmid#46637DepositorUseLentiviralTagsExpressionMutationPromoterhU6Available sinceAug. 29, 2013AvailabilityAcademic Institutions and Nonprofits only -
AB.pCCL.sin.cPPT.U6.miR-330-Decoy.hPGK.GFP.WPRE
Plasmid#46611DepositorUseLentiviralTagsExpressionMutationPromoterhU6Available sinceAug. 29, 2013AvailabilityAcademic Institutions and Nonprofits only -
AB.pCCL.sin.cPPT.U6.miR-744-Decoy.hPGK.GFP.WPRE
Plasmid#46633DepositorUseLentiviralTagsExpressionMutationPromoterhU6Available sinceAug. 29, 2013AvailabilityAcademic Institutions and Nonprofits only -
AB.pCCL.sin.cPPT.U6.miR-195-Decoy.hPGK.GFP.WPRE
Plasmid#46598DepositorUseLentiviralTagsExpressionMutationPromoterhU6Available sinceAug. 29, 2013AvailabilityAcademic Institutions and Nonprofits only -
MT1G
Plasmid#105704PurposeBacterial expression plasmids for production of recombinant metallothionein 1gDepositorInsertMetallothionein 1g (MT1G Human)
UseTagsChiting Binding Domanin-InteinExpressionBacterialMutationPromoterAvailable sinceApril 27, 2020AvailabilityAcademic Institutions and Nonprofits only -
MT1X
Plasmid#105709PurposeBacterial expression plasmids for production of recombinant metallothionein 1xDepositorInsertMetallothionein 1x (MT1X Human)
UseTagsChiting Binding Domanin-InteinExpressionBacterialMutationPromoterAvailable sinceApril 27, 2020AvailabilityAcademic Institutions and Nonprofits only -
MT1F
Plasmid#105703PurposeBacterial expression plasmids for production of recombinant metallothionein 1fDepositorInsertMetallothionein 1f (MT1F Human)
UseTagsChiting Binding Domanin-InteinExpressionBacterialMutationPromoterAvailable sinceApril 27, 2020AvailabilityAcademic Institutions and Nonprofits only -
pUC119
Plasmid#50010PurposepUC cloning vectorDepositorTypeEmpty backboneUseCloning vectorTagsExpressionMutationPromoterAvailable sinceJuly 18, 2014AvailabilityAcademic Institutions and Nonprofits only -
pUC118
Plasmid#50009PurposepUC cloning vectorDepositorTypeEmpty backboneUseCloning vectorTagsExpressionMutationPromoterAvailable sinceJuly 18, 2014AvailabilityAcademic Institutions and Nonprofits only -
pT3TS
Plasmid#31830DepositorTypeEmpty backboneUseTagsExpressionMutationPromoterLacAvailable sinceAug. 29, 2011AvailabilityAcademic Institutions and Nonprofits only -
pGAL4Kan
Plasmid#32320DepositorInsertGAL4 Kan
UseTagsExpressionMutationPromoterAvailable sinceSept. 15, 2011AvailabilityAcademic Institutions and Nonprofits only -
pPD301 ZF43x16-Compact_EYFP
Plasmid#138911PurposeEYFP reporter vector for the ZF43x16-Compact promoterDepositorInsertZF43x16-Compact
UseSynthetic BiologyTagsExpressionMammalianMutationPromoterZF43x16-CompactAvailable sinceFeb. 9, 2021AvailabilityAcademic Institutions and Nonprofits only -
pPD299 ZF43x14-Compact_EYFP
Plasmid#138909PurposeEYFP reporter vector for the ZF43x14-Compact promoterDepositorInsertZF43x14-Compact
UseSynthetic BiologyTagsExpressionMammalianMutationPromoterZF43x14-CompactAvailable sinceFeb. 9, 2021AvailabilityAcademic Institutions and Nonprofits only