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Enter a sequence to perform a BLAST-based search of Addgene plasmid sequence databases.

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We narrowed to 23,297 results for: NSI

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Showing: 7641 - 7680 of 23297 results

  3. pIBA3-SpyCatcherEQ-sfGFP

    Plasmid
    #107421
    Purpose
    For production of inactive SpyCatcherEQ protein fused N-terminally to sfGFP. Includes N-terminal His tag for purification and C-terminal StrepII tag for detection.
    Depositor
    Jack Leo
    Article
    Chauhan et al Mol Microbiol. 2019 Jan 1. doi: 10.1111/
    Insert
    SpyCatcher
    Tags
    His tag, StrepII, and superfolder GFP
    Expression
    Bacterial
    Mutation
    mutation of glutamate 77 to glutamine
    Promoter
    Tet
    Available Since
    Jan. 8, 2019
    Availability
    Academic Institutions and Nonprofits only
    107421-plasmid-map-sequence-id-207622

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  6. pEPQD1CB0770

    Plasmid
    #177060
    Purpose
    MoClo Level 1, position 5, transcriptional unit for transient expression of loganic acid O-methyltransferase (CrLAMT) from Catharanthus roseus driven by 35S promoter; contains a chloroplast transit peptide
    Depositor
    Nicola Patron
    Article
    Dudley et al Commun Biol. 2022 Sep 10;5(1):949. doi:
    Insert
    loganic acid O-methyltransferase (CrLAMT) from Catharanthus roseus
    Use
    Synthetic Biology
    Available Since
    Dec. 6, 2021
    Availability
    Academic Institutions and Nonprofits only
    177060-plasmid-map-sequence-id-349517

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  9. pIBA2-SpyTag-MBP

    Plasmid
    #107422
    Purpose
    Control construct for SpyCatcher experiments. Encodes MBP with N-terminal SpyTag, including OmpA signal peptide for periplasmic targeting.
    Depositor
    Jack Leo
    Article
    Chauhan et al Mol Microbiol. 2019 Jan 1. doi: 10.1111/
    Insert
    Maltose-binding protein (MalE)
    Tags
    OmpA signal peptide, SpyTag, and StrepII
    Expression
    Bacterial
    Promoter
    Tet
    Available Since
    Jan. 8, 2019
    Availability
    Academic Institutions and Nonprofits only
    107422-plasmid-map-sequence-id-212955

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  11. pEPQD1CB0109

    Plasmid
    #177042
    Purpose
    MoClo Level 1, position 2, transcriptional unit for transient expression of geranyl pyrophosphate synthase (PaGPPS1) from Picea abies driven by 35S promoter; contains a chloroplast transit peptide
    Depositor
    Nicola Patron
    Article
    Dudley et al Commun Biol. 2022 Sep 10;5(1):949. doi:
    Insert
    geranyl pyrophosphate synthase (PaGPPS1) from Picea abies
    Use
    Synthetic Biology
    Available Since
    Nov. 30, 2021
    Availability
    Academic Institutions and Nonprofits only
    177042-plasmid-map-sequence-id-349018

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  28. pXW109Hg(RS-RinA)

    Plasmid
    #123150
    Purpose
    Mercury sensor with two-layered amplifier cascade. pSB4A3 carrying J109-32merR-t-PmerT-30hrpR-30hrpS-t-PhrpLE-30RinA-t-PrinA-30gfp-t
    Depositor
    Baojun Wang
    Article
    Wan et al Nat Chem Biol. 2019 May;15(5):540-548. d
    Insert
    J109-32merR-t-PmerT-30hrpR-30hrpS-t-PhrpLE-30RinA-t-PrinA-30gfp-t
    Use
    Synthetic Biology
    Expression
    Bacterial
    Promoter
    J109, PmerT, PhrpLE, PrinA
    Available Since
    May 9, 2019
    Availability
    Academic Institutions and Nonprofits only
    123150-plasmid-map-sequence-id-243991

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  30. GFP-CmTMC1truncated

    Plasmid
    #154908
    Purpose
    For the expression and purification of the truncated TMC1 protein from Chelonia mydas. The protease cleavage site was replaced with the HRV 3C site.
    Depositor
    Motoyuki Hattori
    Article
    Jia et al Neuron. 2020 Jan 22;105(2):310-321.e3. d
    Insert
    GFP-CmTMC1truncated
    Tags
    8histidine-EGFP
    Expression
    Insect
    Mutation
    Truncations of the residues 1-243 and 843-969
    Available Since
    Sept. 10, 2020
    Availability
    Academic Institutions and Nonprofits only
    154908-plasmid-map-sequence-id-310626

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  31. GFP-MuTMC2truncated

    Plasmid
    #154909
    Purpose
    For the expression and purification of the truncated TMC2 protein from Melopsittacus undulates. The protease cleavage site was replaced with the HRV 3C site.
    Depositor
    Motoyuki Hattori
    Article
    Jia et al Neuron. 2020 Jan 22;105(2):310-321.e3. d
    Insert
    GFP-MuTMC2truncated
    Tags
    8histidine-EGFP
    Expression
    Insect
    Mutation
    Truncations of the residues 1-179 and 691-716
    Available Since
    July 27, 2020
    Availability
    Academic Institutions and Nonprofits only
    154909-plasmid-map-sequence-id-306870

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  37. p158

    Plasmid
    #62617
    Purpose
    ePathBrick pETM6 vector encoding CsF3H, CsDFR, and DuLAR
    Depositor
    Mattheos Koffas
    Article
    Zhao et al Metab Eng. 2014 Dec 17;28C:43-53. doi: 1
    Inserts
    flavaone 3B-hydroxylase
    dihydroflavonol 4-reductase
    leucoanthocyanidin reductase
    Use
    Synthetic Biology
    Tags
    GBD, PDZ, and SH3
    Expression
    Bacterial
    Promoter
    T7
    Available Since
    July 9, 2015
    Availability
    Academic Institutions and Nonprofits only
    62617-plasmid-map-sequence-id-102961

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Showing: 7641 - 7680 of 23297 results